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P00893

- ILVI_ECOLI

UniProt

P00893 - ILVI_ECOLI

Protein

Acetolactate synthase isozyme 3 large subunit

Gene

ilvI

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2 pyruvate = 2-acetolactate + CO2.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 thiamine pyrophosphate per subunit.By similarity

    Enzyme regulationi

    Sensitive to valine inhibition.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei51 – 511Thiamine pyrophosphateBy similarity
    Binding sitei153 – 1531FADBy similarity
    Metal bindingi448 – 4481MagnesiumBy similarity
    Metal bindingi475 – 4751MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi261 – 28222FADBy similarityAdd
    BLAST
    Nucleotide bindingi304 – 32320FADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. acetolactate synthase activity Source: EcoCyc
    2. flavin adenine dinucleotide binding Source: InterPro
    3. magnesium ion binding Source: EcoCyc
    4. thiamine pyrophosphate binding Source: EcoCyc

    GO - Biological processi

    1. isoleucine biosynthetic process Source: EcoCyc
    2. valine biosynthetic process Source: EcoCyc

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein, Magnesium, Metal-binding, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciEcoCyc:ACETOLACTSYNIII-ICHAIN-MONOMER.
    ECOL316407:JW0076-MONOMER.
    MetaCyc:ACETOLACTSYNIII-ICHAIN-MONOMER.
    UniPathwayiUPA00047; UER00055.
    UPA00049; UER00059.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetolactate synthase isozyme 3 large subunit (EC:2.2.1.6)
    Alternative name(s):
    AHAS-III
    ALS-III
    Acetohydroxy-acid synthase III large subunit
    Gene namesi
    Name:ilvI
    Ordered Locus Names:b0077, JW0076
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10500. ilvI.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 574574Acetolactate synthase isozyme 3 large subunitPRO_0000090789Add
    BLAST

    Proteomic databases

    PaxDbiP00893.
    PRIDEiP00893.

    Expressioni

    Gene expression databases

    GenevestigatoriP00893.

    Interactioni

    Subunit structurei

    Dimer of large and small chains.

    Protein-protein interaction databases

    DIPiDIP-6850N.
    IntActiP00893. 7 interactions.
    STRINGi511145.b0077.

    Structurei

    3D structure databases

    ProteinModelPortaliP00893.
    SMRiP00893. Positions 4-565.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni397 – 47781Thiamine pyrophosphate bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TPP enzyme family.Curated

    Phylogenomic databases

    eggNOGiCOG0028.
    HOGENOMiHOG000258448.
    KOiK01652.
    OMAiKFDKPNR.
    OrthoDBiEOG6KT2NW.
    PhylomeDBiP00893.

    Family and domain databases

    Gene3Di3.40.50.1220. 1 hit.
    3.40.50.970. 2 hits.
    InterProiIPR012846. Acetolactate_synth_lsu.
    IPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR029061. THDP-binding.
    IPR012000. Thiamin_PyroP_enz_cen_dom.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR000399. TPP-bd_CS.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view]
    PfamiPF02775. TPP_enzyme_C. 1 hit.
    PF00205. TPP_enzyme_M. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52467. SSF52467. 1 hit.
    SSF52518. SSF52518. 2 hits.
    TIGRFAMsiTIGR00118. acolac_lg. 1 hit.
    PROSITEiPS00187. TPP_ENZYMES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00893-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEMLSGAEMV VRSLIDQGVK QVFGYPGGAV LDIYDALHTV GGIDHVLVRH    50
    EQAAVHMADG LARATGEVGV VLVTSGPGAT NAITGIATAY MDSIPLVVLS 100
    GQVATSLIGY DAFQECDMVG ISRPVVKHSF LVKQTEDIPQ VLKKAFWLAA 150
    SGRPGPVVVD LPKDILNPAN KLPYVWPESV SMRSYNPTTT GHKGQIKRAL 200
    QTLVAAKKPV VYVGGGAITA GCHQQLKETV EALNLPVVCS LMGLGAFPAT 250
    HRQALGMLGM HGTYEANMTM HNADVIFAVG VRFDDRTTNN LAKYCPNATV 300
    LHIDIDPTSI SKTVTADIPI VGDARQVLEQ MLELLSQESA HQPLDEIRDW 350
    WQQIEQWRAR QCLKYDTHSE KIKPQAVIET LWRLTKGDAY VTSDVGQHQM 400
    FAALYYPFDK PRRWINSGGL GTMGFGLPAA LGVKMALPEE TVVCVTGDGS 450
    IQMNIQELST ALQYELPVLV VNLNNRYLGM VKQWQDMIYS GRHSQSYMQS 500
    LPDFVRLAEA YGHVGIQISH PHELESKLSE ALEQVRNNRL VFVDVTVDGS 550
    EHVYPMQIRG GGMDEMWLSK TERT 574
    Length:574
    Mass (Da):62,984
    Last modified:November 1, 1997 - v2
    Checksum:iB62DEF64338CBF8C
    GO

    Sequence cautioni

    The sequence CAA25755.1 differs from that shown. Reason: Frameshift at position 523.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti202 – 2032TL → SV in CAA25755. (PubMed:6308579)Curated
    Sequence conflicti202 – 2032TL → SV in CAA38854. 1 PublicationCurated
    Sequence conflicti206 – 2061A → V in CAA25755. (PubMed:6308579)Curated
    Sequence conflicti206 – 2061A → V in CAA38854. 1 PublicationCurated
    Sequence conflicti254 – 2541A → V in CAA25755. (PubMed:6308579)Curated
    Sequence conflicti254 – 2541A → V in CAA38854. 1 PublicationCurated
    Sequence conflicti422 – 4221T → S in CAA25755. (PubMed:6308579)Curated
    Sequence conflicti422 – 4221T → S in CAA38854. 1 PublicationCurated
    Sequence conflicti437 – 4382LP → FA in CAA38854. 1 PublicationCurated
    Sequence conflicti507 – 5093LAE → RG in CAA25755. (PubMed:6308579)Curated
    Sequence conflicti507 – 5093LAE → RG in CAA38854. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01609 Genomic DNA. Translation: CAA25755.1. Frameshift.
    U00096 Genomic DNA. Translation: AAC73188.2.
    AP009048 Genomic DNA. Translation: BAB96646.2.
    X55034 Genomic DNA. Translation: CAA38854.1.
    M10738 Genomic DNA. Translation: AAA24026.1.
    PIRiE64729. YCEC3I.
    RefSeqiYP_025294.2. NC_000913.3.
    YP_488383.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73188; AAC73188; b0077.
    BAB96646; BAB96646; BAB96646.
    GeneIDi12930386.
    948793.
    KEGGiecj:Y75_p0077.
    eco:b0077.
    PATRICi32115257. VBIEscCol129921_0080.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01609 Genomic DNA. Translation: CAA25755.1 . Frameshift.
    U00096 Genomic DNA. Translation: AAC73188.2 .
    AP009048 Genomic DNA. Translation: BAB96646.2 .
    X55034 Genomic DNA. Translation: CAA38854.1 .
    M10738 Genomic DNA. Translation: AAA24026.1 .
    PIRi E64729. YCEC3I.
    RefSeqi YP_025294.2. NC_000913.3.
    YP_488383.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P00893.
    SMRi P00893. Positions 4-565.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6850N.
    IntActi P00893. 7 interactions.
    STRINGi 511145.b0077.

    Proteomic databases

    PaxDbi P00893.
    PRIDEi P00893.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73188 ; AAC73188 ; b0077 .
    BAB96646 ; BAB96646 ; BAB96646 .
    GeneIDi 12930386.
    948793.
    KEGGi ecj:Y75_p0077.
    eco:b0077.
    PATRICi 32115257. VBIEscCol129921_0080.

    Organism-specific databases

    EchoBASEi EB0495.
    EcoGenei EG10500. ilvI.

    Phylogenomic databases

    eggNOGi COG0028.
    HOGENOMi HOG000258448.
    KOi K01652.
    OMAi KFDKPNR.
    OrthoDBi EOG6KT2NW.
    PhylomeDBi P00893.

    Enzyme and pathway databases

    UniPathwayi UPA00047 ; UER00055 .
    UPA00049 ; UER00059 .
    BioCyci EcoCyc:ACETOLACTSYNIII-ICHAIN-MONOMER.
    ECOL316407:JW0076-MONOMER.
    MetaCyc:ACETOLACTSYNIII-ICHAIN-MONOMER.

    Miscellaneous databases

    PROi P00893.

    Gene expression databases

    Genevestigatori P00893.

    Family and domain databases

    Gene3Di 3.40.50.1220. 1 hit.
    3.40.50.970. 2 hits.
    InterProi IPR012846. Acetolactate_synth_lsu.
    IPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR029061. THDP-binding.
    IPR012000. Thiamin_PyroP_enz_cen_dom.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR000399. TPP-bd_CS.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view ]
    Pfami PF02775. TPP_enzyme_C. 1 hit.
    PF00205. TPP_enzyme_M. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52467. SSF52467. 1 hit.
    SSF52518. SSF52518. 2 hits.
    TIGRFAMsi TIGR00118. acolac_lg. 1 hit.
    PROSITEi PS00187. TPP_ENZYMES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular structure of ilvIH and its evolutionary relationship to ilvG in Escherichia coli K12."
      Squires C.H., Defelice M., Devereux J., Calvo J.M.
      Nucleic Acids Res. 11:5299-5313(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
      Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
      Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Regulation of transcription at the 2-minute region of the genetic map of Escherichia coli."
      Ayala J.A.
      Submitted (JAN-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "Unusual organization of the ilvIH promoter of Escherichia coli."
      Haughn G.W., Squires C.H., Defelice M., Largo C.T., Calvo J.M.
      J. Bacteriol. 163:186-198(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8.
    7. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
      Link A.J., Robison K., Church G.M.
      Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-12.
      Strain: K12 / EMG2.

    Entry informationi

    Entry nameiILVI_ECOLI
    AccessioniPrimary (citable) accession number: P00893
    Secondary accession number(s): P78045
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 139 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    E.coli contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.
    Contains 1 molecule of FAD per monomer. The role of this cofactor is not clear considering that the reaction does not involve redox chemistry By similarity.By similarity

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3