SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P00890

- CISY1_YEAST

UniProt

P00890 - CISY1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Citrate synthase, mitochondrial

Gene
CIT1, GLU3, LYS6, YNR001C, N2019
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei312 – 3121 By similarity
Active sitei358 – 3581 By similarity
Active sitei413 – 4131 By similarity

GO - Molecular functioni

  1. citrate (Si)-synthase activity Source: SGD

GO - Biological processi

  1. acetyl-CoA catabolic process Source: SGD
  2. cellular carbohydrate metabolic process Source: InterPro
  3. citrate metabolic process Source: SGD
  4. glutamate biosynthetic process Source: SGD
  5. tricarboxylic acid cycle Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciYEAST:YNR001C-MONOMER.
ReactomeiREACT_189012. Mitochondrial protein import.
UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase, mitochondrial (EC:2.3.3.16)
Gene namesi
Name:CIT1
Synonyms:GLU3, LYS6
Ordered Locus Names:YNR001C
ORF Names:N2019
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNR001c.
SGDiS000005284. CIT1.

Subcellular locationi

Mitochondrion matrix 1 Publication

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. mitochondrial intermembrane space Source: Reactome
  3. mitochondrial matrix Source: UniProtKB-SubCell
  4. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3737MitochondrionAdd
BLAST
Chaini38 – 479442Citrate synthase, mitochondrialPRO_0000005479Add
BLAST

Proteomic databases

MaxQBiP00890.
PaxDbiP00890.
PeptideAtlasiP00890.
PRIDEiP00890.

Expressioni

Gene expression databases

GenevestigatoriP00890.

Interactioni

Protein-protein interaction databases

BioGridi35825. 49 interactions.
DIPiDIP-4597N.
IntActiP00890. 5 interactions.
MINTiMINT-567581.
STRINGi4932.YNR001C.

Structurei

3D structure databases

ProteinModelPortaliP00890.
SMRiP00890. Positions 40-478.

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0372.
GeneTreeiENSGT00750000118689.
HOGENOMiHOG000130831.
KOiK01647.
OMAiELIYEDC.
OrthoDBiEOG7WQ82G.

Family and domain databases

Gene3Di1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00890-1 [UniParc]FASTAAdd to Basket

« Hide

MSAILSTTSK SFLSRGSTRQ CQNMQKALFA LLNARHYSSA SEQTLKERFA    50
EIIPAKAEEI KKFKKEHGKT VIGEVLLEQA YGGMRGIKGL VWEGSVLDPE 100
EGIRFRGRTI PEIQRELPKA EGSTEPLPEA LFWLLLTGEI PTDAQVKALS 150
ADLAARSEIP EHVIQLLDSL PKDLHPMAQF SIAVTALESE SKFAKAYAQG 200
VSKKEYWSYT FEDSLDLLGK LPVIASKIYR NVFKDGKITS TDPNADYGKN 250
LAQLLGYENK DFIDLMRLYL TIHSDHEGGN VSAHTTHLVG SALSSPYLSL 300
AAGLNGLAGP LHGRANQEVL EWLFKLREEV KGDYSKETIE KYLWDTLNAG 350
RVVPGYGHAV LRKTDPRYTA QREFALKHFP DYELFKLVST IYEVAPGVLT 400
KHGKTKNPWP NVDSHSGVLL QYYGLTEASF YTVLFGVARA IGVLPQLIID 450
RAVGAPIERP KSFSTEKYKE LVKKIESKN 479
Length:479
Mass (Da):53,360
Last modified:February 1, 1994 - v2
Checksum:i280661B1CB248F14
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581E → Q in CAA25359. 1 Publication
Sequence conflicti78 – 781E → EE in CAA25359. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00782 Genomic DNA. Translation: CAA25359.1.
Z23259 Genomic DNA. Translation: CAA80781.1.
X77395 Genomic DNA. Translation: CAA54569.1.
Z71616 Genomic DNA. Translation: CAA96277.1.
BK006947 Genomic DNA. Translation: DAA10543.1.
PIRiS35390. YKBY.
RefSeqiNP_014398.1. NM_001183178.1.

Genome annotation databases

EnsemblFungiiYNR001C; YNR001C; YNR001C.
GeneIDi855732.
KEGGisce:YNR001C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00782 Genomic DNA. Translation: CAA25359.1 .
Z23259 Genomic DNA. Translation: CAA80781.1 .
X77395 Genomic DNA. Translation: CAA54569.1 .
Z71616 Genomic DNA. Translation: CAA96277.1 .
BK006947 Genomic DNA. Translation: DAA10543.1 .
PIRi S35390. YKBY.
RefSeqi NP_014398.1. NM_001183178.1.

3D structure databases

ProteinModelPortali P00890.
SMRi P00890. Positions 40-478.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35825. 49 interactions.
DIPi DIP-4597N.
IntActi P00890. 5 interactions.
MINTi MINT-567581.
STRINGi 4932.YNR001C.

Proteomic databases

MaxQBi P00890.
PaxDbi P00890.
PeptideAtlasi P00890.
PRIDEi P00890.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNR001C ; YNR001C ; YNR001C .
GeneIDi 855732.
KEGGi sce:YNR001C.

Organism-specific databases

CYGDi YNR001c.
SGDi S000005284. CIT1.

Phylogenomic databases

eggNOGi COG0372.
GeneTreei ENSGT00750000118689.
HOGENOMi HOG000130831.
KOi K01647.
OMAi ELIYEDC.
OrthoDBi EOG7WQ82G.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER00717 .
BioCyci YEAST:YNR001C-MONOMER.
Reactomei REACT_189012. Mitochondrial protein import.

Miscellaneous databases

NextBioi 980117.

Gene expression databases

Genevestigatori P00890.

Family and domain databases

Gene3Di 1.10.580.10. 1 hit.
InterProi IPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view ]
PANTHERi PTHR11739. PTHR11739. 1 hit.
Pfami PF00285. Citrate_synt. 1 hit.
[Graphical view ]
PRINTSi PR00143. CITRTSNTHASE.
SUPFAMi SSF48256. SSF48256. 1 hit.
TIGRFAMsi TIGR01793. cit_synth_euk. 1 hit.
PROSITEi PS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method."
    Suissa M., Suda K., Schatz G.
    EMBO J. 3:1773-1781(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 24657 / D273-10B.
  2. "The effect of point mutations in the hinge of yeast citrate synthase."
    Lindner P., Plueckthun A.
    Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 24657 / D273-10B.
  3. "Organization of the centromeric region of chromosome XIV in Saccharomyces cerevisiae."
    Lalo D., Stettler S., Mariotte S., Gendreau E., Thuriaux P.
    Yeast 10:523-533(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  4. "Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm."
    Verhasselt P., Aert R., Voet M., Volckaert G.
    Yeast 10:1355-1361(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
    Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
    Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCISY1_YEAST
AccessioniPrimary (citable) accession number: P00890
Secondary accession number(s): D6W1H7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1994
Last modified: September 3, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi