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Protein

Citrate synthase, mitochondrial

Gene

CIT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specific citrate synthase with catalytic activity only with acetyl-CoA.1 Publication

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.1 Publication

Kineticsi

  1. KM=76 µM for acetyl-CoA1 Publication

    Pathwayi: tricarboxylic acid cycle

    This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.Curated
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Citrate synthase 3, mitochondrial (CIT3), Citrate synthase, mitochondrial (CIT1), Citrate synthase, peroxisomal (CIT2)
    2. Aconitate hydratase, mitochondrial (ACO1)
    This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei312 – 3121PROSITE-ProRule annotation
    Active sitei358 – 3581PROSITE-ProRule annotation
    Active sitei413 – 4131PROSITE-ProRule annotation

    GO - Molecular functioni

    • citrate (Si)-synthase activity Source: SGD

    GO - Biological processi

    • acetyl-CoA catabolic process Source: SGD
    • citrate metabolic process Source: SGD
    • glutamate biosynthetic process Source: SGD
    • tricarboxylic acid cycle Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Enzyme and pathway databases

    BioCyciYEAST:YNR001C-MONOMER.
    BRENDAi2.3.3.16. 984.
    ReactomeiR-SCE-71403. Citric acid cycle (TCA cycle).
    UniPathwayiUPA00223; UER00717.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Citrate synthase, mitochondrial1 Publication (EC:2.3.3.11 Publication)
    Gene namesi
    Name:CIT11 Publication
    Synonyms:GLU3, LYS6
    Ordered Locus Names:YNR001C
    ORF Names:N2019
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XIV

    Organism-specific databases

    EuPathDBiFungiDB:YNR001C.
    SGDiS000005284. CIT1.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: Reactome
    • mitochondrial intermembrane space Source: Reactome
    • mitochondrial matrix Source: UniProtKB-SubCell
    • mitochondrion Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3737MitochondrionAdd
    BLAST
    Chaini38 – 479442Citrate synthase, mitochondrialPRO_0000005479Add
    BLAST

    Proteomic databases

    MaxQBiP00890.
    PeptideAtlasiP00890.
    PRIDEiP00890.
    TopDownProteomicsiP00890.

    Interactioni

    Protein-protein interaction databases

    BioGridi35825. 51 interactions.
    DIPiDIP-4597N.
    IntActiP00890. 10 interactions.
    MINTiMINT-567581.

    Structurei

    3D structure databases

    ProteinModelPortaliP00890.
    SMRiP00890. Positions 40-478.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the citrate synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    GeneTreeiENSGT00830000129649.
    HOGENOMiHOG000130831.
    InParanoidiP00890.
    KOiK01647.
    OMAiINRTKYW.
    OrthoDBiEOG7WQ82G.

    Family and domain databases

    Gene3Di1.10.580.10. 1 hit.
    InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
    IPR002020. Citrate_synthase.
    IPR019810. Citrate_synthase_AS.
    IPR010109. Citrate_synthase_euk.
    [Graphical view]
    PANTHERiPTHR11739. PTHR11739. 1 hit.
    PfamiPF00285. Citrate_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00143. CITRTSNTHASE.
    SUPFAMiSSF48256. SSF48256. 1 hit.
    TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
    PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00890-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSAILSTTSK SFLSRGSTRQ CQNMQKALFA LLNARHYSSA SEQTLKERFA
    60 70 80 90 100
    EIIPAKAEEI KKFKKEHGKT VIGEVLLEQA YGGMRGIKGL VWEGSVLDPE
    110 120 130 140 150
    EGIRFRGRTI PEIQRELPKA EGSTEPLPEA LFWLLLTGEI PTDAQVKALS
    160 170 180 190 200
    ADLAARSEIP EHVIQLLDSL PKDLHPMAQF SIAVTALESE SKFAKAYAQG
    210 220 230 240 250
    VSKKEYWSYT FEDSLDLLGK LPVIASKIYR NVFKDGKITS TDPNADYGKN
    260 270 280 290 300
    LAQLLGYENK DFIDLMRLYL TIHSDHEGGN VSAHTTHLVG SALSSPYLSL
    310 320 330 340 350
    AAGLNGLAGP LHGRANQEVL EWLFKLREEV KGDYSKETIE KYLWDTLNAG
    360 370 380 390 400
    RVVPGYGHAV LRKTDPRYTA QREFALKHFP DYELFKLVST IYEVAPGVLT
    410 420 430 440 450
    KHGKTKNPWP NVDSHSGVLL QYYGLTEASF YTVLFGVARA IGVLPQLIID
    460 470
    RAVGAPIERP KSFSTEKYKE LVKKIESKN
    Length:479
    Mass (Da):53,360
    Last modified:February 1, 1994 - v2
    Checksum:i280661B1CB248F14
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti58 – 581E → Q in CAA25359 (PubMed:6090126).Curated
    Sequence conflicti78 – 781E → EE in CAA25359 (PubMed:6090126).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X00782 Genomic DNA. Translation: CAA25359.1.
    Z23259 Genomic DNA. Translation: CAA80781.1.
    X77395 Genomic DNA. Translation: CAA54569.1.
    Z71616 Genomic DNA. Translation: CAA96277.1.
    BK006947 Genomic DNA. Translation: DAA10543.1.
    PIRiS35390. YKBY.
    RefSeqiNP_014398.1. NM_001183178.1.

    Genome annotation databases

    EnsemblFungiiYNR001C; YNR001C; YNR001C.
    GeneIDi855732.
    KEGGisce:YNR001C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X00782 Genomic DNA. Translation: CAA25359.1.
    Z23259 Genomic DNA. Translation: CAA80781.1.
    X77395 Genomic DNA. Translation: CAA54569.1.
    Z71616 Genomic DNA. Translation: CAA96277.1.
    BK006947 Genomic DNA. Translation: DAA10543.1.
    PIRiS35390. YKBY.
    RefSeqiNP_014398.1. NM_001183178.1.

    3D structure databases

    ProteinModelPortaliP00890.
    SMRiP00890. Positions 40-478.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi35825. 51 interactions.
    DIPiDIP-4597N.
    IntActiP00890. 10 interactions.
    MINTiMINT-567581.

    Proteomic databases

    MaxQBiP00890.
    PeptideAtlasiP00890.
    PRIDEiP00890.
    TopDownProteomicsiP00890.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYNR001C; YNR001C; YNR001C.
    GeneIDi855732.
    KEGGisce:YNR001C.

    Organism-specific databases

    EuPathDBiFungiDB:YNR001C.
    SGDiS000005284. CIT1.

    Phylogenomic databases

    GeneTreeiENSGT00830000129649.
    HOGENOMiHOG000130831.
    InParanoidiP00890.
    KOiK01647.
    OMAiINRTKYW.
    OrthoDBiEOG7WQ82G.

    Enzyme and pathway databases

    UniPathwayiUPA00223; UER00717.
    BioCyciYEAST:YNR001C-MONOMER.
    BRENDAi2.3.3.16. 984.
    ReactomeiR-SCE-71403. Citric acid cycle (TCA cycle).

    Miscellaneous databases

    NextBioi980117.
    PROiP00890.

    Family and domain databases

    Gene3Di1.10.580.10. 1 hit.
    InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
    IPR002020. Citrate_synthase.
    IPR019810. Citrate_synthase_AS.
    IPR010109. Citrate_synthase_euk.
    [Graphical view]
    PANTHERiPTHR11739. PTHR11739. 1 hit.
    PfamiPF00285. Citrate_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00143. CITRTSNTHASE.
    SUPFAMiSSF48256. SSF48256. 1 hit.
    TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
    PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method."
      Suissa M., Suda K., Schatz G.
      EMBO J. 3:1773-1781(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 24657 / D273-10B.
    2. "The effect of point mutations in the hinge of yeast citrate synthase."
      Lindner P., Plueckthun A.
      Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: ATCC 24657 / D273-10B.
    3. "Organization of the centromeric region of chromosome XIV in Saccharomyces cerevisiae."
      Lalo D., Stettler S., Mariotte S., Gendreau E., Thuriaux P.
      Yeast 10:523-533(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / GRF88.
    4. "Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm."
      Verhasselt P., Aert R., Voet M., Volckaert G.
      Yeast 10:1355-1361(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    7. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
      Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
      Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "Functional comparison of citrate synthase isoforms from S. cerevisiae."
      Graybill E.R., Rouhier M.F., Kirby C.E., Hawes J.W.
      Arch. Biochem. Biophys. 465:26-37(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCISY1_YEAST
    AccessioniPrimary (citable) accession number: P00890
    Secondary accession number(s): D6W1H7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: February 1, 1994
    Last modified: April 13, 2016
    This is version 157 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Citrate synthase is found in nearly all cells capable of oxidative metabolism.Curated

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.