Citrate synthase, mitochondrial precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P00890 (CISY1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 130. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Citrate synthase, mitochondrial
EC=2.3.3.1

Gene names

Name:	CIT1
Synonyms:	GLU3, LYS6
Ordered Locus Names:	YNR001C
ORF Names:	N2019

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H₂O + oxaloacetate = citrate + CoA.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.
Subcellular location	Mitochondrion matrix Ref.7.
Miscellaneous	Citrate synthase is found in nearly all cells capable of oxidative metabolism.
Sequence similarities	Belongs to the citrate synthase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	acetyl-CoA catabolic process Inferred from mutant phenotype PubMed 17570335. Source: SGD cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro citrate metabolic process Traceable author statement PubMed 9175438. Source: SGD glutamate biosynthetic process Traceable author statement PubMed 9175438. Source: SGD tricarboxylic acid cycle Traceable author statement PubMed 9175438. Source: SGD
Cellular_component	cytosol Traceable author statement. Source: Reactome mitochondrial intermembrane space Traceable author statement. Source: Reactome mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	citrate (Si)-synthase activity Inferred from mutant phenotype PubMed 17570335. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 37

Mitochondrion

Chain

38 – 479

442

Citrate synthase, mitochondrial

PRO_0000005479

Sites

Active site

312

By similarity

Active site

358

By similarity

Active site

413

By similarity

Experimental info

Sequence conflict

E → Q in CAA25359. Ref.1

Sequence conflict

E → EE in CAA25359. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P00890 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: 280661B1CB248F14
Show »

FASTA

479

53,360

        10         20         30         40         50         60 
MSAILSTTSK SFLSRGSTRQ CQNMQKALFA LLNARHYSSA SEQTLKERFA EIIPAKAEEI 

        70         80         90        100        110        120 
KKFKKEHGKT VIGEVLLEQA YGGMRGIKGL VWEGSVLDPE EGIRFRGRTI PEIQRELPKA 

       130        140        150        160        170        180 
EGSTEPLPEA LFWLLLTGEI PTDAQVKALS ADLAARSEIP EHVIQLLDSL PKDLHPMAQF 

       190        200        210        220        230        240 
SIAVTALESE SKFAKAYAQG VSKKEYWSYT FEDSLDLLGK LPVIASKIYR NVFKDGKITS 

       250        260        270        280        290        300 
TDPNADYGKN LAQLLGYENK DFIDLMRLYL TIHSDHEGGN VSAHTTHLVG SALSSPYLSL 

       310        320        330        340        350        360 
AAGLNGLAGP LHGRANQEVL EWLFKLREEV KGDYSKETIE KYLWDTLNAG RVVPGYGHAV 

       370        380        390        400        410        420 
LRKTDPRYTA QREFALKHFP DYELFKLVST IYEVAPGVLT KHGKTKNPWP NVDSHSGVLL 

       430        440        450        460        470 
QYYGLTEASF YTVLFGVARA IGVLPQLIID RAVGAPIERP KSFSTEKYKE LVKKIESKN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method." Suissa M., Suda K., Schatz G. EMBO J. 3:1773-1781(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24657 / D273-10B.
[2]	"The effect of point mutations in the hinge of yeast citrate synthase." Lindner P., Plueckthun A. Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 24657 / D273-10B.
[3]	"Organization of the centromeric region of chromosome XIV in Saccharomyces cerevisiae." Lalo D., Stettler S., Mariotte S., Gendreau E., Thuriaux P. Yeast 10:523-533(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[4]	"Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm." Verhasselt P., Aert R., Voet M., Volckaert G. Yeast 10:1355-1361(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M. Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X00782 Genomic DNA. Translation: CAA25359.1. Z23259 Genomic DNA. Translation: CAA80781.1. X77395 Genomic DNA. Translation: CAA54569.1. Z71616 Genomic DNA. Translation: CAA96277.1. BK006947 Genomic DNA. Translation: DAA10543.1.
PIR	YKBY. S35390.
RefSeq	NP_014398.1. NM_001183178.1.
3D structure databases
ProteinModelPortal	P00890.
SMR	P00890. Positions 40-478.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4597N.
IntAct	P00890. 5 interactions.
MINT	MINT-567581.
STRING	4932.YNR001C.
Proteomic databases
PaxDb	P00890.
PeptideAtlas	P00890.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR001C; YNR001C; YNR001C.
GeneID	855732.
KEGG	sce:YNR001C.
Organism-specific databases
CYGD	YNR001c.
SGD	S000005284. CIT1.
Phylogenomic databases
eggNOG	COG0372.
GeneTree	ENSGT00390000006813.
HOGENOM	HOG000130831.
KO	K01647.
OMA	ELIYEDC.
OrthoDB	EOG4HHS9S.
Enzyme and pathway databases
Reactome	REACT_118590. Mitochondrial Protein Import (yeast). REACT_85873. Metabolism of proteins.
UniPathway	UPA00223; UER00717.
Gene expression databases
Genevestigator	P00890.
GermOnline	YNR001C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.580.10. 1 hit.
InterPro	IPR016142. Citrate_synth-like_lrg_a-sub. IPR002020. Citrate_synthase-like. IPR016141. Citrate_synthase-like_core. IPR019810. Citrate_synthase_AS. IPR010109. Citrate_synthase_euk. [Graphical view]
PANTHER	PTHR11739. PTHR11739. 1 hit.
Pfam	PF00285. Citrate_synt. 1 hit. [Graphical view]
PRINTS	PR00143. CITRTSNTHASE.
SUPFAM	SSF48256. Citrate_synthase_core. 1 hit.
TIGRFAMs	TIGR01793. cit_synth_euk. 1 hit.
PROSITE	PS00480. CITRATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980117.

Entry information

Entry name

CISY1_YEAST

Accession

Primary (citable) accession number: P00890
Secondary accession number(s): D6W1H7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 130 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents