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Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive

Gene

aroF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Catalytic activityi

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Cofactori

Enzyme regulationi

Specifically feedback inhibited by tyrosine with 50% inhibition observed at 9 microM tyrosine, pH 7.0.

Pathwayi: chorismate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (aroG)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Quinate/shikimate dehydrogenase (ydiB), Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

  • 3-deoxy-7-phosphoheptulonate synthase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc

GO - Biological processi

  • aromatic amino acid family biosynthetic process Source: EcoCyc
  • chorismate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:AROF-MONOMER.
ECOL316407:JW2582-MONOMER.
MetaCyc:AROF-MONOMER.
UniPathwayiUPA00053; UER00084.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (EC:2.5.1.54)
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene namesi
Name:aroF
Ordered Locus Names:b2601, JW2582
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10078. aroF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitivePRO_0000140831Add
BLAST

Proteomic databases

PaxDbiP00888.
PRIDEiP00888.

2D gel databases

SWISS-2DPAGEP00888.

Interactioni

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4263470. 18 interactions.
DIPiDIP-9154N.
IntActiP00888. 3 interactions.
STRINGi511145.b2601.

Structurei

3D structure databases

ProteinModelPortaliP00888.
SMRiP00888. Positions 9-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I DAHP synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105E99. Bacteria.
COG0722. LUCA.
HOGENOMiHOG000220501.
InParanoidiP00888.
KOiK01626.
OMAiSWETTDA.
PhylomeDBiP00888.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006219. DHAP_synth_1.
[Graphical view]
PANTHERiPTHR21225. PTHR21225. 1 hit.
PfamiPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMsiTIGR00034. aroFGH. 1 hit.

Sequencei

Sequence statusi: Complete.

P00888-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKDALNNVH ITDEQVLMTP EQLKAAFPLS LQQEAQIADS RKSISDIIAG
60 70 80 90 100
RDPRLLVVCG PCSIHDPETA LEYARRFKAL AAEVSDSLYL VMRVYFEKPR
110 120 130 140 150
TTVGWKGLIN DPHMDGSFDV EAGLQIARKL LLELVNMGLP LATEALDPNS
160 170 180 190 200
PQYLGDLFSW SAIGARTTES QTHREMASGL SMPVGFKNGT DGSLATAINA
210 220 230 240 250
MRAAAQPHRF VGINQAGQVA LLQTQGNPDG HVILRGGKAP NYSPADVAQC
260 270 280 290 300
EKEMEQAGLR PSLMVDCSHG NSNKDYRRQP AVAESVVAQI KDGNRSIIGL
310 320 330 340 350
MIESNIHEGN QSSEQPRSEM KYGVSVTDAC ISWEMTDALL REIHQDLNGQ

LTARVA
Length:356
Mass (Da):38,804
Last modified:August 13, 1987 - v1
Checksum:iA675537531FBC37D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01989 Genomic DNA. Translation: AAA23489.1.
M10431 Genomic DNA. Translation: AAA24332.1.
U00096 Genomic DNA. Translation: AAC75650.1.
AP009048 Genomic DNA. Translation: BAA16487.1.
M60890 Genomic DNA. Translation: AAA23491.1.
K03453 Genomic DNA. Translation: AAA23490.1.
PIRiI41141. ADECHY.
RefSeqiNP_417092.1. NC_000913.3.
WP_001168037.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75650; AAC75650; b2601.
BAA16487; BAA16487; BAA16487.
GeneIDi947084.
KEGGiecj:JW2582.
eco:b2601.
PATRICi32120601. VBIEscCol129921_2699.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01989 Genomic DNA. Translation: AAA23489.1.
M10431 Genomic DNA. Translation: AAA24332.1.
U00096 Genomic DNA. Translation: AAC75650.1.
AP009048 Genomic DNA. Translation: BAA16487.1.
M60890 Genomic DNA. Translation: AAA23491.1.
K03453 Genomic DNA. Translation: AAA23490.1.
PIRiI41141. ADECHY.
RefSeqiNP_417092.1. NC_000913.3.
WP_001168037.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP00888.
SMRiP00888. Positions 9-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263470. 18 interactions.
DIPiDIP-9154N.
IntActiP00888. 3 interactions.
STRINGi511145.b2601.

2D gel databases

SWISS-2DPAGEP00888.

Proteomic databases

PaxDbiP00888.
PRIDEiP00888.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75650; AAC75650; b2601.
BAA16487; BAA16487; BAA16487.
GeneIDi947084.
KEGGiecj:JW2582.
eco:b2601.
PATRICi32120601. VBIEscCol129921_2699.

Organism-specific databases

EchoBASEiEB0076.
EcoGeneiEG10078. aroF.

Phylogenomic databases

eggNOGiENOG4105E99. Bacteria.
COG0722. LUCA.
HOGENOMiHOG000220501.
InParanoidiP00888.
KOiK01626.
OMAiSWETTDA.
PhylomeDBiP00888.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00084.
BioCyciEcoCyc:AROF-MONOMER.
ECOL316407:JW2582-MONOMER.
MetaCyc:AROF-MONOMER.

Miscellaneous databases

PROiP00888.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006219. DHAP_synth_1.
[Graphical view]
PANTHERiPTHR21225. PTHR21225. 1 hit.
PfamiPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMsiTIGR00034. aroFGH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROF_ECOLI
AccessioniPrimary (citable) accession number: P00888
Secondary accession number(s): Q47061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: September 7, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 3 DAHP synthases, AroF is feedback-inhibited by Tyr. The other 2 DAHP synthases are Phe- and Trp-sensitive, respectively.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.