P00888 (AROF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive
EC=2.5.1.54

Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase

Gene names

Name:	aroF
Ordered Locus Names:	b2601, JW2582

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).
Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Cofactor	Divalent metal ions.
Enzyme regulation	Specifically feedback inhibited by tyrosine with 50% inhibition observed at 9 microM tyrosine, pH 7.0.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Miscellaneous	There are 3 DAHP synthases, AroF is feedback-inhibited by Tyr. The other 2 DAHP synthases are Phe- and Trp-sensitive, respectively.
Sequence similarities	Belongs to the class-I DAHP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive		PRO_0000140831

Sequences

	Sequence		Length	Mass (Da)
	P00888 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: A675537531FBC37D	FASTA	356	38,804
	10 20 30 40 50 60 MQKDALNNVH ITDEQVLMTP EQLKAAFPLS LQQEAQIADS RKSISDIIAG RDPRLLVVCG 70 80 90 100 110 120 PCSIHDPETA LEYARRFKAL AAEVSDSLYL VMRVYFEKPR TTVGWKGLIN DPHMDGSFDV 130 140 150 160 170 180 EAGLQIARKL LLELVNMGLP LATEALDPNS PQYLGDLFSW SAIGARTTES QTHREMASGL 190 200 210 220 230 240 SMPVGFKNGT DGSLATAINA MRAAAQPHRF VGINQAGQVA LLQTQGNPDG HVILRGGKAP 250 260 270 280 290 300 NYSPADVAQC EKEMEQAGLR PSLMVDCSHG NSNKDYRRQP AVAESVVAQI KDGNRSIIGL 310 320 330 340 350 MIESNIHEGN QSSEQPRSEM KYGVSVTDAC ISWEMTDALL REIHQDLNGQ LTARVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of the aroF gene of Escherichia coli and the amino acid sequence of the encoded protein, the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase." Shultz J., Hermodson M.A., Garner C.C., Herrmann K.M. J. Biol. Chem. 259:9655-9661(1984) [PubMed: 6146618] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K12." Hudson G.S., Davidson B.E. J. Mol. Biol. 180:1023-1051(1984) [PubMed: 6396419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Cloning of an aroF allele encoding a tyrosine-insensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase." Weaver L.M., Herrmann K.M. J. Bacteriol. 172:6581-6584(1990) [PubMed: 1977738] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 124-187.
[7]	"Sequence homology between the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Escherichia coli and hemerythrin from Sipunculida." Herrmann K.M., Shultz J., Hermodson M.A. J. Biol. Chem. 255:7079-7081(1980) [PubMed: 6104668] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-30.
[8]	"Operator mutations of the Escherichia coli aroF gene." Garner C.C., Herrmann K.M. J. Biol. Chem. 260:3820-3825(1985) [PubMed: 2857723] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
[9]	"Overexpression, purification, and characterization of tyrosine-sensitive 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase from Escherichia coli." Ramilo C.A., Evans J.N. Protein Expr. Purif. 9:253-261(1997) [PubMed: 9056491] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K01989 Genomic DNA. Translation: AAA23489.1. M10431 Genomic DNA. Translation: AAA24332.1. U00096 Genomic DNA. Translation: AAC75650.1. AP009048 Genomic DNA. Translation: BAA16487.1. M60890 Genomic DNA. Translation: AAA23491.1. K03453 Genomic DNA. Translation: AAA23490.1.
PIR	ADECHY. I41141.
RefSeq	NP_417092.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P00888.
SMR	P00888. Positions 1-352.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9154N.
IntAct	P00888. 1 interaction.
2D gel databases
SWISS-2DPAGE	P00888.
ECO2DBASE	F038.1. 6TH EDITION.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000474; EBESCP00000000474; EBESCG00000000394. EBESCT00000017355; EBESCP00000016646; EBESCG00000016411.
GeneID	947084.
GenomeReviews	Gene locus JW2582 in contig AP009048_GR. Gene locus b2601 in contig U00096_GR.
KEGG	ecj:JW2582. eco:b2601.
PATRIC	32120601. VBIEscCol129921_2699.
Organism-specific databases
EchoBASE	EB0076.
EcoGene	EG10078. aroF.
Phylogenomic databases
eggNOG	COG0722.
GeneTree	EBGT00050000008977.
HOGENOM	HBG670536.
OMA	SINRIAI.
PhylomeDB	P00888.
ProtClustDB	PRK12755.
Enzyme and pathway databases
BioCyc	EcoCyc:AROF-MONOMER. MetaCyc:AROF-MONOMER.
Gene expression databases
Genevestigator	P00888.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006219. DHAP_synth_1. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01626.
PANTHER	PTHR21225. AroFGH. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
PIRSF	PIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMs	TIGR00034. AroFGH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROF_ECOLI

Accession

Primary (citable) accession number: P00888
Secondary accession number(s): Q47061

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 13, 1987
Last modified:	January 25, 2012
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program