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Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive

Gene

aroH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Catalytic activityi

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Pathwayi: chorismate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (aroG)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Quinate/shikimate dehydrogenase (ydiB), Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

  • 3-deoxy-7-phosphoheptulonate synthase activity Source: EcoliWiki
  • identical protein binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:AROH-MONOMER.
ECOL316407:JW1694-MONOMER.
MetaCyc:AROH-MONOMER.
UniPathwayiUPA00053; UER00084.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (EC:2.5.1.54)
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene namesi
Name:aroH
Ordered Locus Names:b1704, JW1694
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10080. aroH.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitivePRO_0000140840Add
BLAST

Proteomic databases

PaxDbiP00887.
PRIDEiP00887.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F53EBI-1125143,EBI-543750

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4260296. 11 interactions.
DIPiDIP-6846N.
IntActiP00887. 5 interactions.
STRINGi511145.b1704.

Structurei

3D structure databases

ProteinModelPortaliP00887.
SMRiP00887. Positions 6-347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I DAHP synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105E99. Bacteria.
COG0722. LUCA.
HOGENOMiHOG000220501.
InParanoidiP00887.
KOiK01626.
OMAiPCLSWED.
OrthoDBiEOG63JR9W.
PhylomeDBiP00887.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006219. DHAP_synth_1.
[Graphical view]
PANTHERiPTHR21225. PTHR21225. 1 hit.
PfamiPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMsiTIGR00034. aroFGH. 1 hit.

Sequencei

Sequence statusi: Complete.

P00887-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRTDELRTA RIESLVTPAE LALRYPVTPG VATHVTDSRR RIEKILNGED
60 70 80 90 100
KRLLVIIGPC SIHDLTAAME YATRLQSLRN QYQSRLEIVM RTYFEKPRTV
110 120 130 140 150
VGWKGLISDP DLNGSYRVNH GLELARKLLL QVNELGVPTA TEFLDMVTGQ
160 170 180 190 200
FIADLISWGA IGARTTESQI HREMASALSC PVGFKNGTDG NTRIAVDAIR
210 220 230 240 250
AARASHMFLS PDKNGQMTIY QTSGNPYGHI IMRGGKKPNY HADDIAAACD
260 270 280 290 300
TLHEFDLPEH LVVDFSHGNC QKQHRRQLEV CEDICQQIRN GSTAIAGIMA
310 320 330 340
ESFLREGTQK IVGSQPLTYG QSITDPCLGW EDTERLVEKL ASAVDTRF
Length:348
Mass (Da):38,735
Last modified:June 20, 2003 - v4
Checksum:i52C3C482447226E0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti203 – 2053RAS → AQ in AAA23493 (PubMed:2903857).Curated
Sequence conflicti333 – 3342TE → RQ in AAA23493 (PubMed:2903857).Curated
Sequence conflicti333 – 3342TE → RQ in CAA27956 (PubMed:6167722).Curated
Sequence conflicti333 – 3331T → S in AAA23497 (PubMed:1677907).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38266 Genomic DNA. Translation: AAA23497.1.
J04221 Genomic DNA. Translation: AAA23493.1.
U00096 Genomic DNA. Translation: AAC74774.1.
AP009048 Genomic DNA. Translation: BAA15473.1.
V00261 Genomic DNA. Translation: CAA23510.1.
X04373 Genomic DNA. Translation: CAA27956.1.
PIRiH64928. ADECH.
RefSeqiNP_416219.1. NC_000913.3.
WP_001082229.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74774; AAC74774; b1704.
BAA15473; BAA15473; BAA15473.
GeneIDi946229.
KEGGiecj:JW1694.
eco:b1704.
PATRICi32118714. VBIEscCol129921_1775.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38266 Genomic DNA. Translation: AAA23497.1.
J04221 Genomic DNA. Translation: AAA23493.1.
U00096 Genomic DNA. Translation: AAC74774.1.
AP009048 Genomic DNA. Translation: BAA15473.1.
V00261 Genomic DNA. Translation: CAA23510.1.
X04373 Genomic DNA. Translation: CAA27956.1.
PIRiH64928. ADECH.
RefSeqiNP_416219.1. NC_000913.3.
WP_001082229.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP00887.
SMRiP00887. Positions 6-347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260296. 11 interactions.
DIPiDIP-6846N.
IntActiP00887. 5 interactions.
STRINGi511145.b1704.

Proteomic databases

PaxDbiP00887.
PRIDEiP00887.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74774; AAC74774; b1704.
BAA15473; BAA15473; BAA15473.
GeneIDi946229.
KEGGiecj:JW1694.
eco:b1704.
PATRICi32118714. VBIEscCol129921_1775.

Organism-specific databases

EchoBASEiEB0078.
EcoGeneiEG10080. aroH.

Phylogenomic databases

eggNOGiENOG4105E99. Bacteria.
COG0722. LUCA.
HOGENOMiHOG000220501.
InParanoidiP00887.
KOiK01626.
OMAiPCLSWED.
OrthoDBiEOG63JR9W.
PhylomeDBiP00887.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00084.
BioCyciEcoCyc:AROH-MONOMER.
ECOL316407:JW1694-MONOMER.
MetaCyc:AROH-MONOMER.

Miscellaneous databases

PROiP00887.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006219. DHAP_synth_1.
[Graphical view]
PANTHERiPTHR21225. PTHR21225. 1 hit.
PfamiPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMsiTIGR00034. aroFGH. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two promoters control the aroH gene of Escherichia coli."
    Hudson G.S., Rellos P., Davidson B.E.
    Gene 102:87-91(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Mutational analysis of the catalytic and feedback sites of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli."
    Ray J.M., Yanofsky C., Bauerle R.
    J. Bacteriol. 170:5500-5506(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Structure and regulation of aroH, the structural gene for the tryptophan-repressible 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthetase of Escherichia coli."
    Zurawski G., Gunsalus R.P., Brown K.D., Yanofsky C.
    J. Mol. Biol. 145:47-73(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-36 AND 232-348.

Entry informationi

Entry nameiAROH_ECOLI
AccessioniPrimary (citable) accession number: P00887
Secondary accession number(s): P78301
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 20, 2003
Last modified: January 20, 2016
This is version 132 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 3 DAHP synthases, AroH is feedback-inhibited by Trp. The other 2 DAHP synthases are Tyr- and Phe-sensitive, respectively.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.