P00887 (AROH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive
EC=2.5.1.54

Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase

Gene names

Name:	aroH
Ordered Locus Names:	b1704, JW1694

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).
Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Miscellaneous	There are 3 DAHP synthases, AroH is feedback-inhibited by Trp. The other 2 DAHP synthases are Tyr- and Phe-sensitive, respectively.
Sequence similarities	Belongs to the class-I DAHP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive		PRO_0000140840
Experimental info
	Sequence conflict	203 – 205	3	RAS → AQ in AAA23493. Ref.2
	Sequence conflict	333 – 334	2	TE → RQ in AAA23493. Ref.2
	Sequence conflict	333 – 334	2	TE → RQ in CAA27956. Ref.6
	Sequence conflict	333	1	T → S in AAA23497. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P00887 [UniParc]. Last modified June 20, 2003. Version 4. Checksum: 52C3C482447226E0	FASTA	348	38,735
	10 20 30 40 50 60 MNRTDELRTA RIESLVTPAE LALRYPVTPG VATHVTDSRR RIEKILNGED KRLLVIIGPC 70 80 90 100 110 120 SIHDLTAAME YATRLQSLRN QYQSRLEIVM RTYFEKPRTV VGWKGLISDP DLNGSYRVNH 130 140 150 160 170 180 GLELARKLLL QVNELGVPTA TEFLDMVTGQ FIADLISWGA IGARTTESQI HREMASALSC 190 200 210 220 230 240 PVGFKNGTDG NTRIAVDAIR AARASHMFLS PDKNGQMTIY QTSGNPYGHI IMRGGKKPNY 250 260 270 280 290 300 HADDIAAACD TLHEFDLPEH LVVDFSHGNC QKQHRRQLEV CEDICQQIRN GSTAIAGIMA 310 320 330 340 ESFLREGTQK IVGSQPLTYG QSITDPCLGW EDTERLVEKL ASAVDTRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two promoters control the aroH gene of Escherichia coli." Hudson G.S., Rellos P., Davidson B.E. Gene 102:87-91(1991) [PubMed: 1677907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Mutational analysis of the catalytic and feedback sites of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli." Ray J.M., Yanofsky C., Bauerle R. J. Bacteriol. 170:5500-5506(1988) [PubMed: 2903857] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Structure and regulation of aroH, the structural gene for the tryptophan-repressible 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthetase of Escherichia coli." Zurawski G., Gunsalus R.P., Brown K.D., Yanofsky C. J. Mol. Biol. 145:47-73(1981) [PubMed: 6167722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-36 AND 232-348.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M38266 Genomic DNA. Translation: AAA23497.1. J04221 Genomic DNA. Translation: AAA23493.1. U00096 Genomic DNA. Translation: AAC74774.1. AP009048 Genomic DNA. Translation: BAA15473.1. V00261 Genomic DNA. Translation: CAA23510.1. X04373 Genomic DNA. Translation: CAA27956.1.
PIR	ADECH. H64928.
RefSeq	NP_416219.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P00887.
SMR	P00887. Positions 1-347.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6846N.
IntAct	P00887. 5 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001274; EBESCP00000001274; EBESCG00000001056. EBESCT00000001275; EBESCP00000001275; EBESCG00000001056. EBESCT00000017429; EBESCP00000016720; EBESCG00000016485.
GeneID	946229.
GenomeReviews	Gene locus JW1694 in contig AP009048_GR. Gene locus b1704 in contig U00096_GR.
KEGG	ecj:JW1694. eco:b1704.
PATRIC	32118714. VBIEscCol129921_1775.
Organism-specific databases
EchoBASE	EB0078.
EcoGene	EG10080. aroH.
Phylogenomic databases
eggNOG	COG0722.
GeneTree	EBGT00050000008977.
HOGENOM	HBG670536.
OMA	ELFIVMR.
PhylomeDB	P00887.
ProtClustDB	PRK12756.
Enzyme and pathway databases
BioCyc	EcoCyc:AROH-MONOMER. MetaCyc:AROH-MONOMER.
Gene expression databases
Genevestigator	P00887.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006219. DHAP_synth_1. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01626.
PANTHER	PTHR21225. AroFGH. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
PIRSF	PIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMs	TIGR00034. AroFGH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROH_ECOLI

Accession

Primary (citable) accession number: P00887
Secondary accession number(s): P78301

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 20, 2003
Last modified:	January 25, 2012
This is version 103 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program