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P00885

- ALKD_PSEPU

UniProt

P00885 - ALKD_PSEPU

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Protein

2-dehydro-3-deoxy-phosphogluconate aldolase

Gene

eda

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei61 – 611
Active sitei145 – 1451Schiff-base intermediate with substrate1 PublicationPROSITE-ProRule annotation

GO - Molecular functioni

  1. 2-dehydro-3-deoxy-phosphogluconate aldolase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Schiff base

Names & Taxonomyi

Protein namesi
Recommended name:
2-dehydro-3-deoxy-phosphogluconate aldolase (EC:4.1.2.14)
Alternative name(s):
KDPG-aldolase
Phospho-2-dehydro-3-deoxygluconate aldolase
Phospho-2-keto-3-deoxygluconate aldolase
Gene namesi
Name:eda
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 2262252-dehydro-3-deoxy-phosphogluconate aldolasePRO_0000201035Add
BLAST

Interactioni

Subunit structurei

Homotrimer.

Structurei

Secondary structure

1
226
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 2715
Beta strandi28 – 336
Helixi38 – 403
Helixi41 – 5010
Beta strandi55 – 628
Helixi65 – 7511
Beta strandi79 – 846
Helixi89 – 9810
Beta strandi101 – 1044
Helixi110 – 1189
Helixi130 – 1378
Turni138 – 1403
Beta strandi143 – 1464
Helixi149 – 1524
Helixi154 – 1629
Turni163 – 1675
Beta strandi169 – 1757
Turni178 – 1803
Helixi181 – 1866
Beta strandi193 – 1953
Helixi201 – 2055
Helixi209 – 22113

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MXSX-ray2.20A2-226[»]
ProteinModelPortaliP00885.
SMRiP00885. Positions 11-226.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00885.

Family & Domainsi

Sequence similaritiesi

Belongs to the KHG/KDPG aldolase family.Curated

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR000887. Aldlse_KDPG_KHG.
IPR013785. Aldolase_TIM.
[Graphical view]
PfamiPF01081. Aldolase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01182. eda. 1 hit.
PROSITEiPS00159. ALDOLASE_KDPG_KHG_1. 1 hit.
PS00160. ALDOLASE_KDPG_KHG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00885-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTLERPQPK LSMADKAARI DAICEKARIL PVITIAREED ILPLADALAA
60 70 80 90 100
GGIRTLEVTL RSQHGLKAIQ VLREQRPELC VGAGTVLDRS MFAAVEAAGA
110 120 130 140 150
QFVVTPGITE DILEAGVDSE IPLLPGISTP SEIMMGYALG YRRFKLFPAE
160 170 180 190 200
ISGGVAAIKA FGGPFGDIRF CPTGGVNPAN VRNYMALPNV MCVGTGWMLD
210 220
SSWIKNGDWA RIEACSAEAI ALLDAN
Length:226
Mass (Da):24,069
Last modified:January 23, 2007 - v2
Checksum:i081FE3492F7A1452
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101E → Q in CAC14910. (PubMed:12393206)Curated
Sequence conflicti195 – 1962TG → GT in CAC14910. (PubMed:12393206)Curated
Sequence conflicti220 – 2201I → M in CAC14910. (PubMed:12393206)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ279003 Genomic DNA. Translation: CAC14910.1.
PIRiA01105. ADPSGP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ279003 Genomic DNA. Translation: CAC14910.1 .
PIRi A01105. ADPSGP.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MXS X-ray 2.20 A 2-226 [» ]
ProteinModelPortali P00885.
SMRi P00885. Positions 11-226.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P00885.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR000887. Aldlse_KDPG_KHG.
IPR013785. Aldolase_TIM.
[Graphical view ]
Pfami PF01081. Aldolase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01182. eda. 1 hit.
PROSITEi PS00159. ALDOLASE_KDPG_KHG_1. 1 hit.
PS00160. ALDOLASE_KDPG_KHG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of the zwf-pgl-eda-operon in Pseudomonas putida strains H and KT2440."
    Petruschka L., Adolf K., Burchhardt G., Dernedde J., Jurgensen J., Herrmann H.
    FEMS Microbiol. Lett. 215:89-95(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: H.
  2. "Complete primary structure of 2-keto-3-deoxy-6-phosphogluconate aldolase."
    Suzuki N., Wood W.A.
    J. Biol. Chem. 255:3427-3435(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-226.
  3. "Structure of 2-keto-3-deoxy-6-phosphogluconate aldolase at 2.8-A resolution."
    Mavridis I.M., Hatada M.H., Tulinsky A., Lebioda L.
    J. Mol. Biol. 162:419-444(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
  4. "The folding and quaternary structure of trimeric 2-keto-3-deoxy-6-phosphogluconic aldolase at 3.5-A resolution."
    Mavridis I.M., Tulinsky A.
    Biochemistry 15:4410-4417(1976) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS).

Entry informationi

Entry nameiALKD_PSEPU
AccessioniPrimary (citable) accession number: P00885
Secondary accession number(s): Q9EV78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3