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P00885

- ALKD_PSEPU

UniProt

P00885 - ALKD_PSEPU

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Protein

2-dehydro-3-deoxy-phosphogluconate aldolase

Gene
eda
Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei61 – 611
Active sitei145 – 1451Schiff-base intermediate with substrate1 Publication

GO - Molecular functioni

  1. 2-dehydro-3-deoxy-phosphogluconate aldolase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Schiff base

Names & Taxonomyi

Protein namesi
Recommended name:
2-dehydro-3-deoxy-phosphogluconate aldolase (EC:4.1.2.14)
Alternative name(s):
KDPG-aldolase
Phospho-2-dehydro-3-deoxygluconate aldolase
Phospho-2-keto-3-deoxygluconate aldolase
Gene namesi
Name:eda
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 2262252-dehydro-3-deoxy-phosphogluconate aldolasePRO_0000201035Add
BLAST

Interactioni

Subunit structurei

Homotrimer.

Structurei

Secondary structure

1
226
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 2715
Beta strandi28 – 336
Helixi38 – 403
Helixi41 – 5010
Beta strandi55 – 628
Helixi65 – 7511
Beta strandi79 – 846
Helixi89 – 9810
Beta strandi101 – 1044
Helixi110 – 1189
Helixi130 – 1378
Turni138 – 1403
Beta strandi143 – 1464
Helixi149 – 1524
Helixi154 – 1629
Turni163 – 1675
Beta strandi169 – 1757
Turni178 – 1803
Helixi181 – 1866
Beta strandi193 – 1953
Helixi201 – 2055
Helixi209 – 22113

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MXSX-ray2.20A2-226[»]
ProteinModelPortaliP00885.
SMRiP00885. Positions 11-226.

Miscellaneous databases

EvolutionaryTraceiP00885.

Family & Domainsi

Sequence similaritiesi

Belongs to the KHG/KDPG aldolase family.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR000887. Aldlse_KDPG_KHG.
IPR013785. Aldolase_TIM.
[Graphical view]
PfamiPF01081. Aldolase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01182. eda. 1 hit.
PROSITEiPS00159. ALDOLASE_KDPG_KHG_1. 1 hit.
PS00160. ALDOLASE_KDPG_KHG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00885-1 [UniParc]FASTAAdd to Basket

« Hide

MTTLERPQPK LSMADKAARI DAICEKARIL PVITIAREED ILPLADALAA    50
GGIRTLEVTL RSQHGLKAIQ VLREQRPELC VGAGTVLDRS MFAAVEAAGA 100
QFVVTPGITE DILEAGVDSE IPLLPGISTP SEIMMGYALG YRRFKLFPAE 150
ISGGVAAIKA FGGPFGDIRF CPTGGVNPAN VRNYMALPNV MCVGTGWMLD 200
SSWIKNGDWA RIEACSAEAI ALLDAN 226
Length:226
Mass (Da):24,069
Last modified:January 23, 2007 - v2
Checksum:i081FE3492F7A1452
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101E → Q in CAC14910. 1 Publication
Sequence conflicti195 – 1962TG → GT in CAC14910. 1 Publication
Sequence conflicti220 – 2201I → M in CAC14910. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ279003 Genomic DNA. Translation: CAC14910.1.
PIRiA01105. ADPSGP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ279003 Genomic DNA. Translation: CAC14910.1 .
PIRi A01105. ADPSGP.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MXS X-ray 2.20 A 2-226 [» ]
ProteinModelPortali P00885.
SMRi P00885. Positions 11-226.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P00885.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR000887. Aldlse_KDPG_KHG.
IPR013785. Aldolase_TIM.
[Graphical view ]
Pfami PF01081. Aldolase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01182. eda. 1 hit.
PROSITEi PS00159. ALDOLASE_KDPG_KHG_1. 1 hit.
PS00160. ALDOLASE_KDPG_KHG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of the zwf-pgl-eda-operon in Pseudomonas putida strains H and KT2440."
    Petruschka L., Adolf K., Burchhardt G., Dernedde J., Jurgensen J., Herrmann H.
    FEMS Microbiol. Lett. 215:89-95(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: H.
  2. "Complete primary structure of 2-keto-3-deoxy-6-phosphogluconate aldolase."
    Suzuki N., Wood W.A.
    J. Biol. Chem. 255:3427-3435(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-226.
  3. "Structure of 2-keto-3-deoxy-6-phosphogluconate aldolase at 2.8-A resolution."
    Mavridis I.M., Hatada M.H., Tulinsky A., Lebioda L.
    J. Mol. Biol. 162:419-444(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
  4. "The folding and quaternary structure of trimeric 2-keto-3-deoxy-6-phosphogluconic aldolase at 3.5-A resolution."
    Mavridis I.M., Tulinsky A.
    Biochemistry 15:4410-4417(1976) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS).

Entry informationi

Entry nameiALKD_PSEPU
AccessioniPrimary (citable) accession number: P00885
Secondary accession number(s): Q9EV78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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