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Protein

Fructose-bisphosphate aldolase B

Gene

Aldob

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561Substrate
Binding sitei147 – 1471Substrate
Active sitei188 – 1881Proton acceptorBy similarity
Active sitei230 – 2301Schiff-base intermediate with dihydroxyacetone-P
Sitei364 – 3641Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate

GO - Molecular functioni

  • fructose-bisphosphate aldolase activity Source: RGD
  • phosphatidylcholine binding Source: RGD

GO - Biological processi

  • cellular response to extracellular stimulus Source: RGD
  • cellular response to insulin stimulus Source: RGD
  • glycolytic process Source: RGD
  • liver development Source: RGD
  • response to amino acid Source: RGD
  • response to cAMP Source: RGD
  • response to carbohydrate Source: RGD
  • response to copper ion Source: RGD
  • response to drug Source: RGD
  • response to fructose Source: RGD
  • response to glucocorticoid Source: RGD
  • response to interleukin-6 Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to peptide hormone Source: RGD
  • response to starvation Source: RGD
  • response to zinc ion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

SABIO-RKP00884.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase B (EC:4.1.2.13)
Alternative name(s):
Liver-type aldolase
Gene namesi
Name:Aldob
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2090. Aldob.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • intracellular membrane-bounded organelle Source: RGD
  • lysosome Source: RGD
  • microtubule organizing center Source: UniProtKB-SubCell
  • nucleus Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • plasma membrane Source: RGD
  • rough endoplasmic reticulum membrane Source: RGD
  • smooth endoplasmic reticulum membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 364363Fructose-bisphosphate aldolase BPRO_0000216943Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei13 – 131N6-succinyllysineBy similarity
Modified residuei36 – 361PhosphoserineBy similarity
Modified residuei39 – 391PhosphothreonineBy similarity
Modified residuei89 – 891PhosphoserineBy similarity
Modified residuei119 – 1191PhosphothreonineBy similarity
Modified residuei121 – 1211N6-succinyllysineBy similarity
Modified residuei132 – 1321PhosphoserineBy similarity
Modified residuei272 – 2721PhosphoserineBy similarity
Modified residuei276 – 2761PhosphoserineBy similarity
Modified residuei309 – 3091PhosphoserineBy similarity
Modified residuei317 – 3171N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP00884.
PRIDEiP00884.

PTM databases

PhosphoSiteiP00884.

Interactioni

Subunit structurei

Homotetramer. Interacts with BBS1, BBS2, BBS4 and BBS7 (By similarity).By similarity

Protein-protein interaction databases

IntActiP00884. 1 interaction.
MINTiMINT-3376760.
STRINGi10116.ENSRNOP00000009111.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N30model-A1-364[»]
ProteinModelPortaliP00884.
SMRiP00884. Positions 2-360.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3588.
HOGENOMiHOG000220876.
HOVERGENiHBG002386.
InParanoidiP00884.
PhylomeDBiP00884.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00884-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHRFPALTS EQKKELSEIA QRIVANGKGI LAADESVGTM GNRLQRIKVE
60 70 80 90 100
NTEENRRQFR ELLFSVDNSI SQSIGGVILF HETLYQKDSQ GKLFRNILKE
110 120 130 140 150
KGIVVGIKLD QGGAPLAGTN KETTIQGLDG LSERCAQYKK DGVDFGKWRA
160 170 180 190 200
VLRISDQCPS SLAIQENANA LARYASICQQ NGLVPIVEPE VLPDGDHDLE
210 220 230 240 250
HCQYVSEKVL AAVYKALNDH HVYLEGTLLK PNMLTAGHAC TKKYTPEQVA
260 270 280 290 300
MATVTALHRT VPAAVPSICF LSGGMSEEDA TLNLNAIYRC PLPRPWKLSF
310 320 330 340 350
SYGRALQASA LAAWGGKAAN KKATQEAFMK RAVANCQAAQ GQYVHTGSSG
360
AASTQSLFTA SYTY
Length:364
Mass (Da):39,618
Last modified:January 23, 2007 - v2
Checksum:iE120FC119F0180F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti234 – 2341L → V in CAA26156 (PubMed:2580098).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10149 mRNA. Translation: AAA40716.1.
X02284
, X02285, X02286, X02287, X02288, X02289, X02290, X02291 Genomic DNA. Translation: CAA26156.1.
V01223 mRNA. Translation: CAA24533.1.
PIRiA22585. ADRTB.
UniGeneiRn.98207.

Genome annotation databases

UCSCiRGD:2090. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10149 mRNA. Translation: AAA40716.1.
X02284
, X02285, X02286, X02287, X02288, X02289, X02290, X02291 Genomic DNA. Translation: CAA26156.1.
V01223 mRNA. Translation: CAA24533.1.
PIRiA22585. ADRTB.
UniGeneiRn.98207.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N30model-A1-364[»]
ProteinModelPortaliP00884.
SMRiP00884. Positions 2-360.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00884. 1 interaction.
MINTiMINT-3376760.
STRINGi10116.ENSRNOP00000009111.

PTM databases

PhosphoSiteiP00884.

Proteomic databases

PaxDbiP00884.
PRIDEiP00884.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2090. rat.

Organism-specific databases

RGDi2090. Aldob.

Phylogenomic databases

eggNOGiCOG3588.
HOGENOMiHOG000220876.
HOVERGENiHBG002386.
InParanoidiP00884.
PhylomeDBiP00884.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
SABIO-RKP00884.

Miscellaneous databases

PROiP00884.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of rat liver aldolase B messenger RNA."
    Tsutsumi K., Mukai T., Tsutsumi R., Mori M., Daimon M., Tanaka T., Yatsuki H., Hori K., Ishikawa K.
    J. Biol. Chem. 259:14572-14575(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structure and genomic organization of the rat aldolase B gene."
    Tsutsumi K., Mukai T., Tsutsumi R., Hidaka S., Arai Y., Hori K., Ishikawa K.
    J. Mol. Biol. 181:153-160(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  3. "Rat aldolase isozyme gene."
    Tsutsumi K., Mukai T., Hidaka S., Miyahara H., Tsutsumi R., Tanaka T., Hori K., Ishikawa K.
    J. Biol. Chem. 258:6537-6542(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 185-364.
  4. "Key enzymes of carbohydrate metabolism as targets of the 11.5-kDa Zn(2+)-binding protein (parathymosin)."
    Brand I.A., Heinickel A.
    J. Biol. Chem. 266:20984-20989(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 41-68.

Entry informationi

Entry nameiALDOB_RAT
AccessioniPrimary (citable) accession number: P00884
Secondary accession number(s): P70706
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In vertebrates, 3 forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.