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P00884

- ALDOB_RAT

UniProt

P00884 - ALDOB_RAT

Protein

Fructose-bisphosphate aldolase B

Gene

Aldob

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei56 – 561Substrate
    Binding sitei147 – 1471Substrate
    Active sitei188 – 1881Proton acceptorBy similarity
    Active sitei230 – 2301Schiff-base intermediate with dihydroxyacetone-P
    Sitei364 – 3641Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: RGD
    2. phosphatidylcholine binding Source: RGD

    GO - Biological processi

    1. cellular response to extracellular stimulus Source: RGD
    2. cellular response to insulin stimulus Source: RGD
    3. glycolytic process Source: RGD
    4. liver development Source: RGD
    5. response to amino acid Source: RGD
    6. response to cAMP Source: RGD
    7. response to carbohydrate Source: RGD
    8. response to copper ion Source: RGD
    9. response to drug Source: RGD
    10. response to fructose Source: RGD
    11. response to glucocorticoid Source: RGD
    12. response to interleukin-6 Source: RGD
    13. response to organic cyclic compound Source: RGD
    14. response to peptide hormone Source: RGD
    15. response to starvation Source: RGD
    16. response to zinc ion Source: RGD

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    SABIO-RKP00884.
    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase B (EC:4.1.2.13)
    Alternative name(s):
    Liver-type aldolase
    Gene namesi
    Name:Aldob
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2090. Aldob.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. cytosol Source: RGD
    3. intracellular membrane-bounded organelle Source: RGD
    4. lysosome Source: RGD
    5. microtubule organizing center Source: UniProtKB-SubCell
    6. nucleus Source: RGD
    7. perinuclear region of cytoplasm Source: RGD
    8. plasma membrane Source: RGD
    9. rough endoplasmic reticulum membrane Source: RGD
    10. smooth endoplasmic reticulum membrane Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 364363Fructose-bisphosphate aldolase BPRO_0000216943Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei13 – 131N6-succinyllysineBy similarity
    Modified residuei36 – 361PhosphoserineBy similarity
    Modified residuei39 – 391PhosphothreonineBy similarity
    Modified residuei121 – 1211N6-succinyllysineBy similarity
    Modified residuei317 – 3171N6-succinyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP00884.
    PRIDEiP00884.

    PTM databases

    PhosphoSiteiP00884.

    Expressioni

    Gene expression databases

    GenevestigatoriP00884.

    Interactioni

    Subunit structurei

    Homotetramer. Interacts with BBS1, BBS2, BBS4 and BBS7 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP00884. 1 interaction.
    MINTiMINT-3376760.

    Structurei

    Secondary structure

    1
    364
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 2315
    Helixi24 – 263
    Beta strandi28 – 325
    Helixi36 – 4611
    Helixi52 – 6312
    Helixi67 – 715
    Beta strandi73 – 786
    Helixi80 – 845
    Beta strandi88 – 903
    Helixi93 – 1008
    Beta strandi103 – 1075
    Beta strandi111 – 1144
    Beta strandi118 – 1203
    Beta strandi122 – 1254
    Helixi130 – 14011
    Beta strandi144 – 1518
    Helixi160 – 17920
    Beta strandi183 – 1908
    Helixi198 – 21821
    Helixi223 – 2253
    Helixi245 – 25915
    Beta strandi266 – 2694
    Helixi276 – 28813
    Beta strandi295 – 3028
    Helixi303 – 31311
    Helixi317 – 3193
    Helixi320 – 33920
    Beta strandi344 – 3463
    Beta strandi352 – 3565

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1N30model-A1-364[»]
    ProteinModelPortaliP00884.
    SMRiP00884. Positions 2-360.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG3588.
    HOGENOMiHOG000220876.
    HOVERGENiHBG002386.
    InParanoidiP00884.
    PhylomeDBiP00884.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view]
    PfamiPF00274. Glycolytic. 1 hit.
    [Graphical view]
    PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00884-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAHRFPALTS EQKKELSEIA QRIVANGKGI LAADESVGTM GNRLQRIKVE    50
    NTEENRRQFR ELLFSVDNSI SQSIGGVILF HETLYQKDSQ GKLFRNILKE 100
    KGIVVGIKLD QGGAPLAGTN KETTIQGLDG LSERCAQYKK DGVDFGKWRA 150
    VLRISDQCPS SLAIQENANA LARYASICQQ NGLVPIVEPE VLPDGDHDLE 200
    HCQYVSEKVL AAVYKALNDH HVYLEGTLLK PNMLTAGHAC TKKYTPEQVA 250
    MATVTALHRT VPAAVPSICF LSGGMSEEDA TLNLNAIYRC PLPRPWKLSF 300
    SYGRALQASA LAAWGGKAAN KKATQEAFMK RAVANCQAAQ GQYVHTGSSG 350
    AASTQSLFTA SYTY 364
    Length:364
    Mass (Da):39,618
    Last modified:January 23, 2007 - v2
    Checksum:iE120FC119F0180F8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti234 – 2341L → V in CAA26156. (PubMed:2580098)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10149 mRNA. Translation: AAA40716.1.
    X02284
    , X02285, X02286, X02287, X02288, X02289, X02290, X02291 Genomic DNA. Translation: CAA26156.1.
    V01223 mRNA. Translation: CAA24533.1.
    PIRiA22585. ADRTB.
    UniGeneiRn.98207.

    Genome annotation databases

    UCSCiRGD:2090. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10149 mRNA. Translation: AAA40716.1 .
    X02284
    , X02285 , X02286 , X02287 , X02288 , X02289 , X02290 , X02291 Genomic DNA. Translation: CAA26156.1 .
    V01223 mRNA. Translation: CAA24533.1 .
    PIRi A22585. ADRTB.
    UniGenei Rn.98207.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1N30 model - A 1-364 [» ]
    ProteinModelPortali P00884.
    SMRi P00884. Positions 2-360.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P00884. 1 interaction.
    MINTi MINT-3376760.

    PTM databases

    PhosphoSitei P00884.

    Proteomic databases

    PaxDbi P00884.
    PRIDEi P00884.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:2090. rat.

    Organism-specific databases

    RGDi 2090. Aldob.

    Phylogenomic databases

    eggNOGi COG3588.
    HOGENOMi HOG000220876.
    HOVERGENi HBG002386.
    InParanoidi P00884.
    PhylomeDBi P00884.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .
    SABIO-RK P00884.

    Gene expression databases

    Genevestigatori P00884.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view ]
    Pfami PF00274. Glycolytic. 1 hit.
    [Graphical view ]
    PROSITEi PS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of rat liver aldolase B messenger RNA."
      Tsutsumi K., Mukai T., Tsutsumi R., Mori M., Daimon M., Tanaka T., Yatsuki H., Hori K., Ishikawa K.
      J. Biol. Chem. 259:14572-14575(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structure and genomic organization of the rat aldolase B gene."
      Tsutsumi K., Mukai T., Tsutsumi R., Hidaka S., Arai Y., Hori K., Ishikawa K.
      J. Mol. Biol. 181:153-160(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
    3. "Rat aldolase isozyme gene."
      Tsutsumi K., Mukai T., Hidaka S., Miyahara H., Tsutsumi R., Tanaka T., Hori K., Ishikawa K.
      J. Biol. Chem. 258:6537-6542(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 185-364.
    4. "Key enzymes of carbohydrate metabolism as targets of the 11.5-kDa Zn(2+)-binding protein (parathymosin)."
      Brand I.A., Heinickel A.
      J. Biol. Chem. 266:20984-20989(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 41-68.

    Entry informationi

    Entry nameiALDOB_RAT
    AccessioniPrimary (citable) accession number: P00884
    Secondary accession number(s): P70706
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In vertebrates, 3 forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3