Reviewed, UniProtKB/Swiss-Prot P00870 (RBS1_SPIOL)

Names and origin

Protein names

Recommended name:
    Ribulose bisphosphate carboxylase small chain
      Short name=RuBisCO small subunit
    EC=4.1.1.39

Gene names

Name:

RBCS

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	123 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.
Subunit structure	8 large chains + 8 small chains.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the RuBisCO small chain family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation Photorespiration Photosynthesis
Cellular component	Chloroplast Plastid
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	carbon utilization by fixation of carbon dioxide Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photorespiration Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 123

123

Ribulose bisphosphate carboxylase small chain

PRO_0000198589

Amino acid modifications

Modified residue

1

1

Methionine derivative

Natural variations

Natural variant

94

1

P → Y

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

123

Helix Strand Turn

Details...

Turn

14 – 17

4

Helix

23 – 35

13

Beta strand

39 – 47

9

Beta strand

68 – 71

4

Helix

80 – 93

14

Beta strand

97 – 105

9

Turn

106 – 109

4

Beta strand

110 – 118

9

Sequences

	Sequence		Length	Mass (Da)
	P00870-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: DC727DD6EF1EF59B	FASTA	123	14,283
	10 20 30 40 50 60 MQVWPPLGLK KFETLSYLPP LTTEQLLAEV NYLLVKGWIP PLEFEVKDGF VYREHDKSPG 70 80 90 100 110 120 YYDGRYWTMW KLPMFGGTDP AQVVNEVEEV KKAPPDAFVR FIGFNDKREV QCISFIAYKP AGY

References

[1]	"Amino acid sequence of the small subunit of ribulose-1,5-bisphosphate carboxylase from spinach." Martin P.G. Aust. J. Plant Physiol. 6:401-408(1979) Cited for: PROTEIN SEQUENCE.
[2]	Martin P.G. Submitted (OCT-1982) to the PIR data bank Cited for: SEQUENCE REVISION TO 68-70.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

The Plant Kingdom's sloth - Issue 38 of September 2003

Cross-references

Sequence databases

PIR

RKSPS. A01089.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RBO	X-ray	2.30	C/F/I/S	1-123	[»]
1RCO	X-ray	2.30	C/F/I/M/P/S/T/W	1-123	[»]
1RCX	X-ray	2.40	C/F/I/M/P/S/T/W	1-123	[»]
1RXO	X-ray	2.20	C/F/I/S	1-123	[»]
8RUC	X-ray	1.60	I/J/K/L	1-123	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

4.1.1.39. 286.

Family and domain databases

InterPro

IPR000894. RuBisCO_sc.
[Graphical view]

Gene3D

G3DSA:3.30.190.10. RuBisCO_small. 1 hit.

Pfam

PF00101. RuBisCO_small. 1 hit.
[Graphical view]

PRINTS

PR00152. RUBISCOSMALL.

ProtoNet

Search...

Entry information

Entry name

RBS1_SPIOL

Accession

Primary (citable) accession number: P00870

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	February 9, 2010
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)