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P00870

- RBS1_SPIOL

UniProt

P00870 - RBS1_SPIOL

Protein

Ribulose bisphosphate carboxylase small chain

Gene

RBCS

Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

    GO - Molecular functioni

    1. monooxygenase activity Source: UniProtKB-KW
    2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbon fixation Source: UniProtKB-KW
    2. photorespiration Source: UniProtKB-KW
    3. photosynthesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase, Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Carbon dioxide fixation, Photorespiration, Photosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylase small chain (EC:4.1.1.39)
    Short name:
    RuBisCO small subunit
    Gene namesi
    Name:RBCS
    OrganismiSpinacia oleracea (Spinach)
    Taxonomic identifieri3562 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Protein family/group databases

    Allergomei3814. Spi o RuBisCO.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 123123Ribulose bisphosphate carboxylase small chainPRO_0000198589Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11Methionine derivative

    Proteomic databases

    ProMEXiP00870.

    Interactioni

    Subunit structurei

    8 large chains + 8 small chains.

    Protein-protein interaction databases

    DIPiDIP-27640N.

    Structurei

    Secondary structure

    1
    123
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni14 – 174
    Helixi23 – 3513
    Beta strandi39 – 479
    Beta strandi68 – 714
    Helixi80 – 9314
    Beta strandi97 – 1059
    Turni106 – 1094
    Beta strandi110 – 1189

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1RBOX-ray2.30C/F/I/S1-123[»]
    1RCOX-ray2.30C/F/I/M/P/S/T/W1-123[»]
    1RCXX-ray2.40C/F/I/M/P/S/T/W1-123[»]
    1RXOX-ray2.20C/F/I/S1-123[»]
    8RUCX-ray1.60I/J/K/L1-123[»]
    ProteinModelPortaliP00870.
    SMRiP00870. Positions 1-123.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00870.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuBisCO small chain family.Curated

    Family and domain databases

    Gene3Di3.30.190.10. 1 hit.
    InterProiIPR024681. RuBisCO_sc.
    IPR000894. RuBisCO_sc_dom.
    [Graphical view]
    PfamiPF00101. RuBisCO_small. 1 hit.
    [Graphical view]
    PRINTSiPR00152. RUBISCOSMALL.
    SUPFAMiSSF55239. SSF55239. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P00870-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQVWPPLGLK KFETLSYLPP LTTEQLLAEV NYLLVKGWIP PLEFEVKDGF    50
    VYREHDKSPG YYDGRYWTMW KLPMFGGTDP AQVVNEVEEV KKAPPDAFVR 100
    FIGFNDKREV QCISFIAYKP AGY 123
    Length:123
    Mass (Da):14,283
    Last modified:July 21, 1986 - v1
    Checksum:iDC727DD6EF1EF59B
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti94 – 941P → Y.

    Sequence databases

    PIRiA01089. RKSPS.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    The Plant Kingdom's sloth - Issue 38 of September 2003

    Sequence databases

    PIRi A01089. RKSPS.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1RBO X-ray 2.30 C/F/I/S 1-123 [» ]
    1RCO X-ray 2.30 C/F/I/M/P/S/T/W 1-123 [» ]
    1RCX X-ray 2.40 C/F/I/M/P/S/T/W 1-123 [» ]
    1RXO X-ray 2.20 C/F/I/S 1-123 [» ]
    8RUC X-ray 1.60 I/J/K/L 1-123 [» ]
    ProteinModelPortali P00870.
    SMRi P00870. Positions 1-123.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-27640N.

    Protein family/group databases

    Allergomei 3814. Spi o RuBisCO.

    Proteomic databases

    ProMEXi P00870.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P00870.

    Family and domain databases

    Gene3Di 3.30.190.10. 1 hit.
    InterProi IPR024681. RuBisCO_sc.
    IPR000894. RuBisCO_sc_dom.
    [Graphical view ]
    Pfami PF00101. RuBisCO_small. 1 hit.
    [Graphical view ]
    PRINTSi PR00152. RUBISCOSMALL.
    SUPFAMi SSF55239. SSF55239. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Amino acid sequence of the small subunit of ribulose-1,5-bisphosphate carboxylase from spinach."
      Martin P.G.
      Aust. J. Plant Physiol. 6:401-408(1979)
      Cited for: PROTEIN SEQUENCE.
    2. Martin P.G.
      Submitted (OCT-1982) to the PIR data bank
      Cited for: SEQUENCE REVISION TO 68-70.

    Entry informationi

    Entry nameiRBS1_SPIOL
    AccessioniPrimary (citable) accession number: P00870
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3