P00869 (RBS2_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribulose bisphosphate carboxylase small chain 3C, chloroplastic
Short name=RuBisCO small subunit 3C
EC=4.1.1.39

Alternative name(s):
PSS15

Gene names

Name:

RBCS-3C

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.
Subunit structure	8 large chains + 8 small chains.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the RuBisCO small chain family.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation Photorespiration Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Lyase Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological_process	photorespiration Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 57	57	Chloroplast By similarity
	Chain	58 – 180	123	Ribulose bisphosphate carboxylase small chain 3C, chloroplastic		PRO_0000031541
Experimental info
	Sequence conflict	25	1	A → G in AAA33686. Ref.3
	Sequence conflict	32	1	S → F in AAA33686. Ref.3
	Sequence conflict	117	1	G → R in AAA33686. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P00869 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 212653E76E55205C	FASTA	180	20,244
	10 20 30 40 50 60 MASMISSSAV TTVSRASRGQ SAAVAPFGGL KSMTGFPVKK VNTDITSITS NGGRVKCMQV 70 80 90 100 110 120 WPPIGKKKFE TLSYLPPLTR DQLLKEVEYL LRKGWVPCLE FELEKGFVYR EHNKSPGYYD 130 140 150 160 170 180 GRYWTMWKLP MFGTTDASQV LKELDEVVAA YPQAFVRIIG FDNVRQVQCI SFIAHTPESY

References

[1]	"Tissue-specific and light-regulated expression of a pea nuclear gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase." Coruzzi G., Broglie R., Edwards C., Chua N.-H. EMBO J. 3:1671-1679(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (PSS15). Strain: cv. Progress No. 9.
[2]	"Expression dynamics of the pea rbcS multigene family and organ distribution of the transcripts." Fluhr R., Moses P., Morelli G., Coruzzi G., Chua N.-H. EMBO J. 5:2063-2071(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (RBCS-3C).
[3]	"Nucleotide sequences of two pea cDNA clones encoding the small subunit of ribulose 1,5-bisphosphate carboxylase and the major chlorophyll a/b-binding thylakoid polypeptide." Coruzzi G., Broglie R., Cashmore A., Chua N.-H. J. Biol. Chem. 258:1399-1402(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-180.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J01257 mRNA. Translation: AAA33686.1. X00806 Genomic DNA. Translation: CAA25390.1. X04334 Genomic DNA. Translation: CAA27865.1.
PIR	RKPMS5. A01088.
3D structure databases
ProteinModelPortal	P00869.
SMR	P00869. Positions 58-180.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.190.10. 1 hit.
InterPro	IPR024681. RuBisCO_sc. IPR000894. RuBisCO_sc_dom. IPR024680. RuBisCO_ssu_N. [Graphical view]
Pfam	PF12338. RbcS. 1 hit. PF00101. RuBisCO_small. 1 hit. [Graphical view]
PRINTS	PR00152. RUBISCOSMALL.
SUPFAM	SSF55239. RuBisCO_small. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RBS2_PEA

Accession

Primary (citable) accession number: P00869

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program