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Protein

Phosphoenolpyruvate carboxylase

Gene

ppc

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.

Cofactori

Mg2+By similarity

Enzyme regulationi

The enzyme has distinct binding sites for each of the allosteric effectors such as acetyl-CoA, fructose 1,6-bisphosphate, guanosine 3'-diphosphate 5'-diphosphate, long chain fatty acids, and L-aspartate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1381
Active sitei546By similarity1

GO - Molecular functioni

  • magnesium ion binding Source: UniProtKB-HAMAP
  • phosphoenolpyruvate carboxylase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbon dioxide fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciEcoCyc:PEPCARBOX-MONOMER.
ECOL316407:JW3928-MONOMER.
MetaCyc:PEPCARBOX-MONOMER.
BRENDAi4.1.1.31. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxylase (EC:4.1.1.31)
Short name:
PEPC
Short name:
PEPCase
Gene namesi
Name:ppc
Synonyms:glu
Ordered Locus Names:b3956, JW3928
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10756. ppc.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi138H → N: Loss of activity. 1 Publication1
Mutagenesisi579H → N: 29% of wild-type activity. 1 Publication1
Mutagenesisi579H → P: 5.4% of wild-type activity. 1 Publication1
Mutagenesisi587R → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001665921 – 883Phosphoenolpyruvate carboxylaseAdd BLAST883

Proteomic databases

EPDiP00864.
PaxDbiP00864.
PRIDEiP00864.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi4262649. 8 interactors.
DIPiDIP-10538N.
IntActiP00864. 3 interactors.
STRINGi511145.b3956.

Structurei

Secondary structure

1883
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 26Combined sources21
Turni27 – 29Combined sources3
Helixi30 – 46Combined sources17
Helixi50 – 61Combined sources12
Helixi65 – 91Combined sources27
Turni93 – 96Combined sources4
Helixi102 – 112Combined sources11
Helixi119 – 127Combined sources9
Beta strandi131 – 135Combined sources5
Beta strandi143 – 145Combined sources3
Helixi148 – 161Combined sources14
Helixi168 – 187Combined sources20
Helixi198 – 211Combined sources14
Helixi213 – 231Combined sources19
Beta strandi243 – 247Combined sources5
Turni249 – 251Combined sources3
Helixi261 – 289Combined sources29
Helixi297 – 303Combined sources7
Beta strandi307 – 310Combined sources4
Helixi311 – 334Combined sources24
Helixi349 – 365Combined sources17
Helixi369 – 372Combined sources4
Helixi375 – 385Combined sources11
Turni386 – 389Combined sources4
Beta strandi390 – 398Combined sources9
Helixi399 – 412Combined sources14
Helixi418 – 420Combined sources3
Helixi423 – 435Combined sources13
Helixi449 – 463Combined sources15
Beta strandi468 – 475Combined sources8
Helixi480 – 491Combined sources12
Turni492 – 494Combined sources3
Beta strandi501 – 505Combined sources5
Helixi508 – 523Combined sources16
Helixi525 – 530Combined sources6
Turni531 – 533Combined sources3
Beta strandi534 – 539Combined sources6
Helixi541 – 548Combined sources8
Helixi550 – 571Combined sources22
Beta strandi574 – 579Combined sources6
Helixi584 – 586Combined sources3
Helixi589 – 597Combined sources9
Turni601 – 606Combined sources6
Beta strandi608 – 612Combined sources5
Helixi614 – 616Combined sources3
Helixi617 – 621Combined sources5
Helixi624 – 643Combined sources20
Helixi651 – 672Combined sources22
Helixi678 – 685Combined sources8
Helixi688 – 693Combined sources6
Beta strandi697 – 699Combined sources3
Helixi709 – 711Combined sources3
Helixi714 – 723Combined sources10
Helixi728 – 730Combined sources3
Turni731 – 733Combined sources3
Helixi734 – 742Combined sources9
Turni743 – 745Combined sources3
Helixi747 – 756Combined sources10
Helixi758 – 773Combined sources16
Helixi776 – 786Combined sources11
Turni789 – 791Combined sources3
Helixi792 – 812Combined sources21
Turni818 – 821Combined sources4
Helixi823 – 856Combined sources34
Helixi862 – 879Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FIYX-ray2.80A1-883[»]
1JQNX-ray2.35A1-883[»]
1QB4X-ray2.60A1-883[»]
ProteinModelPortaliP00864.
SMRiP00864.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00864.

Family & Domainsi

Sequence similaritiesi

Belongs to the PEPCase type 1 family.Curated

Phylogenomic databases

eggNOGiENOG4105CCA. Bacteria.
COG2352. LUCA.
HOGENOMiHOG000238648.
InParanoidiP00864.
KOiK01595.
OMAiIAEAWHT.
PhylomeDBiP00864.

Family and domain databases

HAMAPiMF_00595. PEPcase_type1. 1 hit.
InterProiIPR021135. PEP_COase.
IPR022805. PEP_COase_bac/pln-type.
IPR018129. PEP_COase_Lys_AS.
IPR033129. PEPCASE_His_AS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00311. PEPcase. 1 hit.
[Graphical view]
PRINTSiPR00150. PEPCARBXLASE.
SUPFAMiSSF51621. SSF51621. 1 hit.
PROSITEiPS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00864-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEQYSALRS NVSMLGKVLG ETIKDALGEH ILERVETIRK LSKSSRAGND
60 70 80 90 100
ANRQELLTTL QNLSNDELLP VARAFSQFLN LANTAEQYHS ISPKGEAASN
110 120 130 140 150
PEVIARTLRK LKNQPELSED TIKKAVESLS LELVLTAHPT EITRRTLIHK
160 170 180 190 200
MVEVNACLKQ LDNKDIADYE HNQLMRRLRQ LIAQSWHTDE IRKLRPSPVD
210 220 230 240 250
EAKWGFAVVE NSLWQGVPNY LRELNEQLEE NLGYKLPVEF VPVRFTSWMG
260 270 280 290 300
GDRDGNPNVT ADITRHVLLL SRWKATDLFL KDIQVLVSEL SMVEATPELL
310 320 330 340 350
ALVGEEGAAE PYRYLMKNLR SRLMATQAWL EARLKGEELP KPEGLLTQNE
360 370 380 390 400
ELWEPLYACY QSLQACGMGI IANGDLLDTL RRVKCFGVPL VRIDIRQEST
410 420 430 440 450
RHTEALGELT RYLGIGDYES WSEADKQAFL IRELNSKRPL LPRNWQPSAE
460 470 480 490 500
TREVLDTCQV IAEAPQGSIA AYVISMAKTP SDVLAVHLLL KEAGIGFAMP
510 520 530 540 550
VAPLFETLDD LNNANDVMTQ LLNIDWYRGL IQGKQMVMIG YSDSAKDAGV
560 570 580 590 600
MAASWAQYQA QDALIKTCEK AGIELTLFHG RGGSIGRGGA PAHAALLSQP
610 620 630 640 650
PGSLKGGLRV TEQGEMIRFK YGLPEITVSS LSLYTGAILE ANLLPPPEPK
660 670 680 690 700
ESWRRIMDEL SVISCDVYRG YVRENKDFVP YFRSATPEQE LGKLPLGSRP
710 720 730 740 750
AKRRPTGGVE SLRAIPWIFA WTQNRLMLPA WLGAGTALQK VVEDGKQSEL
760 770 780 790 800
EAMCRDWPFF STRLGMLEMV FAKADLWLAE YYDQRLVDKA LWPLGKELRN
810 820 830 840 850
LQEEDIKVVL AIANDSHLMA DLPWIAESIQ LRNIYTDPLN VLQAELLHRS
860 870 880
RQAEKEGQEP DPRVEQALMV TIAGIAAGMR NTG
Length:883
Mass (Da):99,063
Last modified:July 21, 1986 - v1
Checksum:i2620A776CF1F2EBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05903 Genomic DNA. Translation: CAA29332.1.
X55417 Genomic DNA. No translation available.
U00006 Genomic DNA. Translation: AAC43062.1.
U00096 Genomic DNA. Translation: AAC76938.1.
AP009048 Genomic DNA. Translation: BAE77355.1.
X01700 Genomic DNA. Translation: CAA25847.1.
PIRiA01083. QYEC.
RefSeqiNP_418391.1. NC_000913.3.
WP_001005586.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76938; AAC76938; b3956.
BAE77355; BAE77355; BAE77355.
GeneIDi948457.
KEGGiecj:JW3928.
eco:b3956.
PATRICi32123433. VBIEscCol129921_4077.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05903 Genomic DNA. Translation: CAA29332.1.
X55417 Genomic DNA. No translation available.
U00006 Genomic DNA. Translation: AAC43062.1.
U00096 Genomic DNA. Translation: AAC76938.1.
AP009048 Genomic DNA. Translation: BAE77355.1.
X01700 Genomic DNA. Translation: CAA25847.1.
PIRiA01083. QYEC.
RefSeqiNP_418391.1. NC_000913.3.
WP_001005586.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FIYX-ray2.80A1-883[»]
1JQNX-ray2.35A1-883[»]
1QB4X-ray2.60A1-883[»]
ProteinModelPortaliP00864.
SMRiP00864.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262649. 8 interactors.
DIPiDIP-10538N.
IntActiP00864. 3 interactors.
STRINGi511145.b3956.

Proteomic databases

EPDiP00864.
PaxDbiP00864.
PRIDEiP00864.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76938; AAC76938; b3956.
BAE77355; BAE77355; BAE77355.
GeneIDi948457.
KEGGiecj:JW3928.
eco:b3956.
PATRICi32123433. VBIEscCol129921_4077.

Organism-specific databases

EchoBASEiEB0749.
EcoGeneiEG10756. ppc.

Phylogenomic databases

eggNOGiENOG4105CCA. Bacteria.
COG2352. LUCA.
HOGENOMiHOG000238648.
InParanoidiP00864.
KOiK01595.
OMAiIAEAWHT.
PhylomeDBiP00864.

Enzyme and pathway databases

BioCyciEcoCyc:PEPCARBOX-MONOMER.
ECOL316407:JW3928-MONOMER.
MetaCyc:PEPCARBOX-MONOMER.
BRENDAi4.1.1.31. 2026.

Miscellaneous databases

EvolutionaryTraceiP00864.
PROiP00864.

Family and domain databases

HAMAPiMF_00595. PEPcase_type1. 1 hit.
InterProiIPR021135. PEP_COase.
IPR022805. PEP_COase_bac/pln-type.
IPR018129. PEP_COase_Lys_AS.
IPR033129. PEPCASE_His_AS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00311. PEPcase. 1 hit.
[Graphical view]
PRINTSiPR00150. PEPCARBXLASE.
SUPFAMiSSF51621. SSF51621. 1 hit.
PROSITEiPS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAPP_ECOLI
AccessioniPrimary (citable) accession number: P00864
Secondary accession number(s): Q2M8Q1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.