P00856 (ATP8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP synthase protein 8

Alternative name(s):
A6L
ATP-associated protein 1
F-ATPase subunit 8

Gene names

Name:	ATP8
Synonyms:	AAP1
Ordered Locus Names:	Q0080

Encoded on

Mitochondrion

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	48 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane By similarity.
Subunit structure	F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. In yeast, the dimeric form of ATP synthase consists of 17 polypeptides: alpha, beta, gamma, delta, epsilon, 4 (B), 5 (OSCP), 6 (A), 8, 9 (C), d, E (Tim11), f, g, h, i/j and k.
Subcellular location	Mitochondrion membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the ATPase protein 8 family.

Ontologies

Keywords
Biological process	ATP synthesis Hydrogen ion transport Ion transport Transport
Cellular component	CF(0) Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled proton transport Inferred from direct assay. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) Inferred from mutant phenotype. Source: SGD
Molecular function	hydrogen ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 48	48	ATP synthase protein 8		PRO_0000195607
Regions
	Transmembrane	13 – 32	20	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P00856 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 7E5483656089A4C2	FASTA	48	5,822
	10 20 30 40 MPQLVPFYFM NQLTYGFLLM ITLLILFSQF FLPMILRLYV SRLFISKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biogenesis of mitochondria: the mitochondrial gene (aap1) coding for mitochondrial ATPase subunit 8 in Saccharomyces cerevisiae." McReadie I.G., Novitski C.E., Maxwell R.J., John U., Ooi B.-G., McMullen G.L., Lukins H.B., Linnane A.W., Nagley P. Nucleic Acids Res. 11:4435-4451(1983) [PubMed: 6223276] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Organization and processing of the mitochondrial oxi3/oli2 multigenic transcript in yeast." Simon M., Faye G. Mol. Gen. Genet. 196:266-274(1984) [PubMed: 6387398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae." Foury F., Roganti T., Lecrenier N., Purnelle B. FEBS Lett. 440:325-331(1998) [PubMed: 9872396] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X00895 Genomic DNA. Translation: CAA25423.1. X00960 Genomic DNA. Translation: CAA25473.1. AJ011856 Genomic DNA. Translation: CAA09831.1.
PIR	EWBY8. S05820.
RefSeq	NP_009312.1. NM_001184359.1.
3D structure databases
ProteinModelPortal	P00856.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3040N.
IntAct	P00856. 3 interactions.
MINT	MINT-655778.
STRING	P00856.
Protein family/group databases
TCDB	3.A.2.1.3. H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	Q0080; Q0080; Q0080.
GeneID	854600.
KEGG	sce:Q0080.
NMPDR	fig|4932.3.peg.1864.
Organism-specific databases
CYGD	Q0080.
SGD	S000007267. AAP1.
Phylogenomic databases
eggNOG	fuNOG13344.
HOGENOM	HBG398772.
OrthoDB	EOG4R53CN.
Gene expression databases
ArrayExpress	P00856.
Genevestigator	P00856.
GermOnline	Q0080. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009230. ATPase_F0-cplx_su8_mt_fun. [Graphical view]
KO	K02125.
Pfam	PF05933. Fun_ATP-synt_8. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977086.

Entry information

Entry name

ATP8_YEAST

Accession

Primary (citable) accession number: P00856

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	December 14, 2011
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program