P00854 (ATP6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP synthase subunit a

Alternative name(s):
F-ATPase protein 6

Gene names

Name:	ATP6
Synonyms:	OLI2, OLI4, PHO1
Ordered Locus Names:	Q0085

Encoded on

Mitochondrion

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel; it may play a direct role in the translocation of protons across the membrane.
Subunit structure	F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. In yeast, the dimeric form of ATP synthase consists of 17 polypeptides: alpha, beta, gamma, delta, epsilon, 4 (B), 5 (OSCP), 6 (A), 8, 9 (C), d, E (Tim11), f, g, h, i/j and k.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ATPase A chain family.

Ontologies

Keywords
Biological process	ATP synthesis Hydrogen ion transport Ion transport Transport
Cellular component	CF(0) Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ATP catabolic process Inferred from direct assay. Source: GOC ATP synthesis coupled proton transport Inferred from direct assay. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) Inferred from physical interaction Ref.4. Source: SGD
Molecular function	hydrogen ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 10	10	Removed in mature form		PRO_0000002626
	Chain	11 – 259	249	ATP synthase subunit a		PRO_0000002627
Regions
	Transmembrane	36 – 56	21	Helical; Potential
	Transmembrane	92 – 112	21	Helical; Potential
	Transmembrane	125 – 145	21	Helical; Potential
	Transmembrane	150 – 170	21	Helical; Potential
	Transmembrane	191 – 211	21	Helical; Potential
	Transmembrane	216 – 236	21	Helical; Potential
Natural variations
	Natural variant	171	1	I → M in strain: D273-10B/A1.
	Natural variant	177	1	F → I in strain: D273-10B/A1.
	Natural variant	231	1	M → I in strain: D273-10B/A1.
	Natural variant	241	1	G → S in strain: D273-10B/A1.
	Natural variant	245	1	A → T in strain: D273-10B/A1.
	Natural variant	255 – 256	2	AV → TL in strain: D273-10B/A1.

Sequences

	Sequence		Length	Mass (Da)
	P00854 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: 1A3257747B5A7C57	FASTA	259	29,099
	10 20 30 40 50 60 MFNLLNTYIT SPLDQFEIRT LFGLQSSFID LSCLNLTTFS LYTIIVLLVI TSLYTLTNNN 70 80 90 100 110 120 NKIIGSRWLI SQEAIYDTIM NMTKGQIGGK NWGLYFPMIF TLFMFIFIAN LISMIPYSFA 130 140 150 160 170 180 LSAHLVFIIS LSIVIWLGNT ILGLYKHGWV FFSLFVPAGT PLPLVPLLVI IETLSYFARA 190 200 210 220 230 240 ISLGLRLGSN ILAGHLLMVI LAGLTFNFML INLFTLVFGF VPLAMILAIM MLEFAIGIIQ 250 GYVWAILTAS YLKDAVYLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Assembly of the mitochondrial membrane system: sequence analysis of a yeast mitochondrial ATPase gene containing the oli-2 and oli-4 loci." Macino G., Tzagoloff A. Cell 20:507-517(1980) [PubMed: 6446405] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D273-10B/A1.
[2]	"Sequence of the mitochondrial oli2 gene coding for subunit 6 of the mitochondrial ATPase complex in different strains of Saccharomyces." John U.P., Nagley P. Nucleic Acids Res. 15:366-366(1987) [PubMed: 2950378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JM6.
[3]	"The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae." Foury F., Roganti T., Lecrenier N., Purnelle B. FEBS Lett. 440:325-331(1998) [PubMed: 9872396] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"NH2-terminal sequence of the isolated yeast ATP synthase subunit 6 reveals post-translational cleavage." Michon T., Galante M., Velours J. Eur. J. Biochem. 172:621-625(1988) [PubMed: 2894987] [Abstract] Cited for: PROTEIN SEQUENCE OF 11-32.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J01464 Genomic DNA. Translation: AAA32145.2. V00683 Genomic DNA. Translation: CAA24054.1. X05056 Genomic DNA. Translation: CAA28727.1. AJ011856 Genomic DNA. Translation: CAA09832.1.
PIR	PWBYJ6. A25869.
RefSeq	NP_009313.1. NM_001184360.1.
3D structure databases
ProteinModelPortal	P00854.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3038N.
IntAct	P00854. 1 interaction.
STRING	P00854.
Protein family/group databases
TCDB	3.A.2.1.3. H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) superfamily.
Proteomic databases
PeptideAtlas	P00854.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	Q0085; Q0085; Q0085.
GeneID	854601.
KEGG	sce:Q0085.
NMPDR	fig|4932.3.peg.1865.
Organism-specific databases
CYGD	Q0085.
SGD	S000007268. ATP6.
Phylogenomic databases
eggNOG	fuNOG09725.
HOGENOM	HBG734175.
OrthoDB	EOG4TTKT0.
Gene expression databases
ArrayExpress	P00854.
Genevestigator	P00854.
GermOnline	Q0085. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000568. ATPase_F0-cplx_asu. IPR023011. ATPase_F0-cplx_asu_AS. [Graphical view]
Gene3D	G3DSA:1.20.120.220. ATPase_F0_A. 1 hit.
KO	K02126.
PANTHER	PTHR11410. ATPase_F0_A. 1 hit.
Pfam	PF00119. ATP-synt_A. 1 hit. [Graphical view]
PRINTS	PR00123. ATPASEA.
SUPFAM	SSF81336. ATPase_F0_A. 1 hit.
TIGRFAMs	TIGR01131. ATP_synt_6_or_A. 1 hit.
PROSITE	PS00449. ATPASE_A. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977089.

Entry information

Entry name

ATP6_YEAST

Accession

Primary (citable) accession number: P00854
Secondary accession number(s): Q95A27

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	May 27, 2002
Last modified:	December 14, 2011
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program