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P00817 (IPYR_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 161. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inorganic pyrophosphatase

EC=3.6.1.1
Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase
Gene names
Name:IPP1
Synonyms:PPA, PPA1
Ordered Locus Names:YBR011C
ORF Names:YBR0202
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length287 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Diphosphate + H2O = 2 phosphate.

Cofactor

Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Miscellaneous

Present with 68400 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the PPase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RSP5P399402EBI-9338,EBI-16219

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 287286Inorganic pyrophosphatase
PRO_0000137588

Sites

Active site901Proton donor Ref.8 Ref.9
Metal binding1161Magnesium 1
Metal binding1211Magnesium 1
Metal binding1211Magnesium 2
Metal binding1531Magnesium 1
Binding site791Diphosphate

Amino acid modifications

Modified residue651Phosphothreonine Ref.13
Modified residue2511Phosphothreonine Ref.11 Ref.12
Modified residue2661Phosphoserine Ref.13 Ref.14
Modified residue2861Phosphoserine Ref.11

Experimental info

Sequence conflict411N → D AA sequence Ref.5
Sequence conflict721D → N AA sequence Ref.5
Sequence conflict751Missing AA sequence Ref.5
Sequence conflict1241E → Q AA sequence Ref.5
Sequence conflict1371Q → E AA sequence Ref.5
Sequence conflict1871N → D AA sequence Ref.5
Sequence conflict2251D → N AA sequence Ref.5
Sequence conflict2671P → L in CAA31629. Ref.1

Secondary structure

.................................................................. 287
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00817 [UniParc].

Last modified July 24, 2007. Version 4.
Checksum: 1DC19A702A389BA9

FASTA28732,300
        10         20         30         40         50         60 
MTYTTRQIGA KNTLEYKVYI EKDGKPVSAF HDIPLYADKE NNIFNMVVEI PRWTNAKLEI 

        70         80         90        100        110        120 
TKEETLNPII QDTKKGKLRF VRNCFPHHGY IHNYGAFPQT WEDPNVSHPE TKAVGDNDPI 

       130        140        150        160        170        180 
DVLEIGETIA YTGQVKQVKA LGIMALLDEG ETDWKVIAID INDPLAPKLN DIEDVEKYFP 

       190        200        210        220        230        240 
GLLRATNEWF RIYKIPDGKP ENQFAFSGEA KNKKYALDII KETHDSWKQL IAGKSSDSKG 

       250        260        270        280 
IDLTNVTLPD TPTYSKAASD AIPPASPKAD APIDKSIDKW FFISGSV 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, molecular characterization and chromosome localization of the inorganic pyrophosphatase (PPA) gene from S. cerevisiae."
Kolakowski L.F. Jr., Schloesser M., Cooperman B.S.
Nucleic Acids Res. 16:10441-10452(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 26109 / X2180.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Covalent structural analysis of yeast inorganic pyrophosphatase."
Cohen S.A., Sterner R., Keim P.S., Heinrikson R.L.
J. Biol. Chem. 253:889-897(1978) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-287.
[6]"Protein identifications for a Saccharomyces cerevisiae protein database."
Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S.
Electrophoresis 15:1466-1486(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-36 AND 240-252.
Strain: ATCC 204508 / S288c.
[7]"Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae."
Norbeck J., Blomberg A.
FEMS Microbiol. Lett. 137:1-8(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 240-250.
Strain: ATCC 38531 / Y41.
[8]"Tyrosine-89 is important for enzymatic activity of S. cerevisiae inorganic pyrophosphatase."
Raznikov A.V., Sklyankina V.A., Avaeva S.M.
FEBS Lett. 308:62-64(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: ACTIVE SITE TYR-90.
[9]"Identification of an arginine important for enzymatic activity within the covalent structure of yeast inorganic pyrophosphatase."
Bond M.W., Chiu N.Y., Cooperman B.S.
Biochemistry 19:94-102(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: ACTIVE SITE.
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-251 AND SER-286, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[12]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-251, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[13]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-65 AND SER-266, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"X-ray diffraction study of inorganic pyrophosphatase from baker's yeast at the 3-A resolution."
Arutiunian E.G., Terzian S.S., Voronova A.A., Kuranova I.P., Smirnova E.A., Vainstein B.K., Hohne W.E., Hansen G.
Dokl. Akad. Nauk SSSR 258:1481-1492(1981)
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
[17]"The structural basis for pyrophosphatase catalysis."
Heikinheimo P., Lehtonen J., Baykov A., Lahti R., Cooperman B.S., Goldman A.
Structure 4:1491-1508(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[18]Swaminathan K., Cooperman B.S., Lahti R., Voet D.
Submitted (DEC-1997) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
[19]"The R78K and D117E active-site variants of Saccharomyces cerevisiae soluble inorganic pyrophosphatase: structural studies and mechanistic implications."
Tuominen V., Heikinheimo P., Kajander T., Torkkel T., Hyytia T., Kapyla J., Lahti R., Cooperman B.S., Goldman A.
J. Mol. Biol. 284:1565-1580(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF MUTANTS LYS-79 AND LYS-118.
[20]"Conservation of functional residues between yeast and E. coli inorganic pyrophosphatases."
Lahti R., Kolakowski L.F. Jr., Heinonen J., Vihinen M., Pohjanoksa K., Cooperman B.S.
Biochim. Biophys. Acta 1038:338-345(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY TO E.COLI AND K.LACTIS PPASES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X13253 Genomic DNA. Translation: CAA31629.1.
Z35880 Genomic DNA. Translation: CAA84949.1.
AY692953 Genomic DNA. Translation: AAT92972.1.
BK006936 Genomic DNA. Translation: DAA07132.1.
PIRPWBY. S45864.
RefSeqNP_009565.1. NM_001178359.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
117EX-ray2.15A/B2-287[»]
1E6AX-ray1.90A/B2-287[»]
1E9GX-ray1.15A/B2-287[»]
1HUJX-ray2.10A/B2-282[»]
1HUKX-ray2.20A/B2-282[»]
1M38X-ray1.80A/B1-287[»]
1PYPX-ray3.00A/B2-287[»]
1WGIX-ray2.20A/B2-287[»]
1WGJX-ray2.00A/B2-287[»]
1YPPX-ray2.40A/B2-287[»]
2IHPX-ray1.50A/B2-287[»]
2IK0X-ray1.70A/B2-287[»]
2IK1X-ray1.70A/B2-287[»]
2IK2X-ray1.80A/B2-287[»]
2IK4X-ray1.80A/B2-287[»]
2IK6X-ray1.80A/B2-287[»]
2IK7X-ray1.90A/B2-287[»]
2IK9X-ray1.50A/B2-287[»]
8PRKX-ray1.85A/B1-287[»]
ProteinModelPortalP00817.
SMRP00817. Positions 2-285.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32712. 76 interactions.
DIPDIP-5753N.
IntActP00817. 34 interactions.
MINTMINT-614477.
STRING4932.YBR011C.

2D gel databases

COMPLUYEAST-2DPAGEP00817.
SWISS-2DPAGEP00817.

Proteomic databases

MaxQBP00817.
PaxDbP00817.
PeptideAtlasP00817.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR011C; YBR011C; YBR011C.
GeneID852296.
KEGGsce:YBR011C.

Organism-specific databases

SGDS000000215. IPP1.

Phylogenomic databases

eggNOGCOG0221.
GeneTreeENSGT00390000017004.
HOGENOMHOG000195569.
KOK01507.
OMAANTLEHR.
OrthoDBEOG7K0ZP6.

Enzyme and pathway databases

BioCycYEAST:YBR011C-MONOMER.

Gene expression databases

GenevestigatorP00817.

Family and domain databases

Gene3D3.90.80.10. 1 hit.
InterProIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERPTHR10286. PTHR10286. 1 hit.
PfamPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMSSF50324. SSF50324. 1 hit.
PROSITEPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00817.
NextBio970952.
PROP00817.

Entry information

Entry nameIPYR_YEAST
AccessionPrimary (citable) accession number: P00817
Secondary accession number(s): D6VQ12
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 24, 2007
Last modified: July 9, 2014
This is version 161 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references