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Protein

Inorganic pyrophosphatase

Gene

IPP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 68400 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Diphosphate + H2O = 2 phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79Diphosphate1
Active sitei90Proton donor1 Publication1
Metal bindingi116Magnesium 11
Metal bindingi121Magnesium 11
Metal bindingi121Magnesium 21
Metal bindingi153Magnesium 11

GO - Molecular functioni

  • inorganic diphosphatase activity Source: SGD
  • magnesium ion binding Source: InterPro

GO - Biological processi

  • phosphate-containing compound metabolic process Source: SGD

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YBR011C-MONOMER
BRENDAi3.6.1.1 984
ReactomeiR-SCE-379716 Cytosolic tRNA aminoacylation
R-SCE-379726 Mitochondrial tRNA aminoacylation
R-SCE-71737 Pyrophosphate hydrolysis

Names & Taxonomyi

Protein namesi
Recommended name:
Inorganic pyrophosphatase (EC:3.6.1.1)
Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name:
PPase
Gene namesi
Name:IPP1
Synonyms:PPA, PPA1
Ordered Locus Names:YBR011C
ORF Names:YBR0202
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR011C
SGDiS000000215 IPP1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001375882 – 287Inorganic pyrophosphataseAdd BLAST286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65PhosphothreonineCombined sources1
Cross-linki239Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei251PhosphothreonineCombined sources1
Modified residuei266PhosphoserineCombined sources1
Cross-linki279Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei286PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP00817
PaxDbiP00817
PRIDEiP00817
TopDownProteomicsiP00817

2D gel databases

COMPLUYEAST-2DPAGEiP00817
SWISS-2DPAGEiP00817

PTM databases

iPTMnetiP00817

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399402EBI-9338,EBI-16219

Protein-protein interaction databases

BioGridi32712, 391 interactors
DIPiDIP-5753N
IntActiP00817, 49 interactors
MINTiP00817
STRINGi4932.YBR011C

Chemistry databases

BindingDBiP00817

Structurei

Secondary structure

1287
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 10Combined sources8
Beta strandi13 – 15Combined sources3
Beta strandi17 – 22Combined sources6
Beta strandi25 – 27Combined sources3
Turni29 – 32Combined sources4
Beta strandi35 – 38Combined sources4
Turni39 – 42Combined sources4
Beta strandi43 – 50Combined sources8
Beta strandi58 – 60Combined sources3
Beta strandi62 – 64Combined sources3
Beta strandi69 – 71Combined sources3
Beta strandi85 – 87Combined sources3
Beta strandi91 – 96Combined sources6
Beta strandi106 – 108Combined sources3
Turni109 – 112Combined sources4
Beta strandi113 – 115Combined sources3
Beta strandi117 – 119Combined sources3
Beta strandi121 – 124Combined sources4
Beta strandi126 – 128Combined sources3
Beta strandi135 – 144Combined sources10
Beta strandi146 – 148Combined sources3
Beta strandi155 – 160Combined sources6
Beta strandi163 – 165Combined sources3
Helixi166 – 168Combined sources3
Helixi172 – 178Combined sources7
Helixi182 – 192Combined sources11
Helixi195 – 197Combined sources3
Helixi205 – 208Combined sources4
Beta strandi210 – 212Combined sources3
Helixi213 – 231Combined sources19
Beta strandi245 – 247Combined sources3
Helixi256 – 258Combined sources3
Turni259 – 261Combined sources3
Beta strandi266 – 268Combined sources3
Helixi275 – 278Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
117EX-ray2.15A/B2-287[»]
1E6AX-ray1.90A/B2-287[»]
1E9GX-ray1.15A/B2-287[»]
1HUJX-ray2.10A/B2-282[»]
1HUKX-ray2.20A/B2-282[»]
1M38X-ray1.80A/B1-287[»]
1PYPX-ray3.00A/B2-287[»]
1WGIX-ray2.20A/B2-287[»]
1WGJX-ray2.00A/B2-287[»]
1YPPX-ray2.40A/B2-287[»]
2IHPX-ray1.50A/B2-287[»]
2IK0X-ray1.70A/B2-287[»]
2IK1X-ray1.70A/B2-287[»]
2IK2X-ray1.80A/B2-287[»]
2IK4X-ray1.80A/B2-287[»]
2IK6X-ray1.80A/B2-287[»]
2IK7X-ray1.90A/B2-287[»]
2IK9X-ray1.50A/B2-287[»]
8PRKX-ray1.85A/B1-287[»]
ProteinModelPortaliP00817
SMRiP00817
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00817

Family & Domainsi

Sequence similaritiesi

Belongs to the PPase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000017004
HOGENOMiHOG000195569
InParanoidiP00817
KOiK01507
OMAiKLRYVRN
OrthoDBiEOG092C3VG0

Family and domain databases

CDDicd00412 pyrophosphatase, 1 hit
Gene3Di3.90.80.10, 1 hit
InterProiView protein in InterPro
IPR008162 Pyrophosphatase
IPR036649 Pyrophosphatase_sf
PANTHERiPTHR10286 PTHR10286, 1 hit
PfamiView protein in Pfam
PF00719 Pyrophosphatase, 1 hit
SUPFAMiSSF50324 SSF50324, 1 hit
PROSITEiView protein in PROSITE
PS00387 PPASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYTTRQIGA KNTLEYKVYI EKDGKPVSAF HDIPLYADKE NNIFNMVVEI
60 70 80 90 100
PRWTNAKLEI TKEETLNPII QDTKKGKLRF VRNCFPHHGY IHNYGAFPQT
110 120 130 140 150
WEDPNVSHPE TKAVGDNDPI DVLEIGETIA YTGQVKQVKA LGIMALLDEG
160 170 180 190 200
ETDWKVIAID INDPLAPKLN DIEDVEKYFP GLLRATNEWF RIYKIPDGKP
210 220 230 240 250
ENQFAFSGEA KNKKYALDII KETHDSWKQL IAGKSSDSKG IDLTNVTLPD
260 270 280
TPTYSKAASD AIPPASPKAD APIDKSIDKW FFISGSV
Length:287
Mass (Da):32,300
Last modified:July 24, 2007 - v4
Checksum:i1DC19A702A389BA9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41N → D AA sequence (PubMed:340461).Curated1
Sequence conflicti72D → N AA sequence (PubMed:340461).Curated1
Sequence conflicti75Missing AA sequence (PubMed:340461).Curated1
Sequence conflicti124E → Q AA sequence (PubMed:340461).Curated1
Sequence conflicti137Q → E AA sequence (PubMed:340461).Curated1
Sequence conflicti187N → D AA sequence (PubMed:340461).Curated1
Sequence conflicti225D → N AA sequence (PubMed:340461).Curated1
Sequence conflicti267P → L in CAA31629 (PubMed:2849749).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13253 Genomic DNA Translation: CAA31629.1
Z35880 Genomic DNA Translation: CAA84949.1
AY692953 Genomic DNA Translation: AAT92972.1
BK006936 Genomic DNA Translation: DAA07132.1
PIRiS45864 PWBY
RefSeqiNP_009565.1, NM_001178359.1

Genome annotation databases

EnsemblFungiiYBR011C; YBR011C; YBR011C
GeneIDi852296
KEGGisce:YBR011C

Similar proteinsi

Entry informationi

Entry nameiIPYR_YEAST
AccessioniPrimary (citable) accession number: P00817
Secondary accession number(s): D6VQ12
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 24, 2007
Last modified: May 23, 2018
This is version 193 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

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