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Protein

Inorganic pyrophosphatase

Gene

IPP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Diphosphate + H2O = 2 phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79Diphosphate1
Active sitei90Proton donor1 Publication1
Metal bindingi116Magnesium 11
Metal bindingi121Magnesium 11
Metal bindingi121Magnesium 21
Metal bindingi153Magnesium 11

GO - Molecular functioni

  • inorganic diphosphatase activity Source: SGD
  • magnesium ion binding Source: InterPro

GO - Biological processi

  • phosphate-containing compound metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YBR011C-MONOMER.
BRENDAi3.6.1.1. 984.
ReactomeiR-SCE-379716. Cytosolic tRNA aminoacylation.
R-SCE-379726. Mitochondrial tRNA aminoacylation.
R-SCE-71737. Pyrophosphate hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Inorganic pyrophosphatase (EC:3.6.1.1)
Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name:
PPase
Gene namesi
Name:IPP1
Synonyms:PPA, PPA1
Ordered Locus Names:YBR011C
ORF Names:YBR0202
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR011C.
SGDiS000000215. IPP1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001375882 – 287Inorganic pyrophosphataseAdd BLAST286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65PhosphothreonineCombined sources1
Cross-linki239Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei251PhosphothreonineCombined sources1
Modified residuei266PhosphoserineCombined sources1
Cross-linki279Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei286PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP00817.
PRIDEiP00817.
TopDownProteomicsiP00817.

2D gel databases

COMPLUYEAST-2DPAGEP00817.
SWISS-2DPAGEP00817.

PTM databases

iPTMnetiP00817.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399402EBI-9338,EBI-16219

Protein-protein interaction databases

BioGridi32712. 84 interactors.
DIPiDIP-5753N.
IntActiP00817. 34 interactors.
MINTiMINT-614477.

Structurei

Secondary structure

1287
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 10Combined sources8
Beta strandi13 – 15Combined sources3
Beta strandi17 – 22Combined sources6
Beta strandi25 – 27Combined sources3
Turni29 – 32Combined sources4
Beta strandi35 – 38Combined sources4
Turni39 – 42Combined sources4
Beta strandi43 – 50Combined sources8
Beta strandi58 – 60Combined sources3
Beta strandi62 – 64Combined sources3
Beta strandi69 – 71Combined sources3
Beta strandi75 – 77Combined sources3
Beta strandi85 – 87Combined sources3
Beta strandi91 – 96Combined sources6
Beta strandi106 – 108Combined sources3
Turni109 – 112Combined sources4
Beta strandi113 – 115Combined sources3
Beta strandi117 – 119Combined sources3
Beta strandi121 – 124Combined sources4
Beta strandi126 – 128Combined sources3
Beta strandi135 – 144Combined sources10
Beta strandi146 – 148Combined sources3
Beta strandi155 – 160Combined sources6
Beta strandi163 – 165Combined sources3
Helixi166 – 168Combined sources3
Helixi172 – 178Combined sources7
Helixi182 – 192Combined sources11
Helixi195 – 197Combined sources3
Helixi205 – 208Combined sources4
Beta strandi210 – 212Combined sources3
Helixi213 – 231Combined sources19
Beta strandi245 – 247Combined sources3
Helixi256 – 258Combined sources3
Turni259 – 261Combined sources3
Beta strandi266 – 268Combined sources3
Helixi275 – 278Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
117EX-ray2.15A/B2-287[»]
1E6AX-ray1.90A/B2-287[»]
1E9GX-ray1.15A/B2-287[»]
1HUJX-ray2.10A/B2-282[»]
1HUKX-ray2.20A/B2-282[»]
1M38X-ray1.80A/B1-287[»]
1PYPX-ray3.00A/B2-287[»]
1WGIX-ray2.20A/B2-287[»]
1WGJX-ray2.00A/B2-287[»]
1YPPX-ray2.40A/B2-287[»]
2IHPX-ray1.50A/B2-287[»]
2IK0X-ray1.70A/B2-287[»]
2IK1X-ray1.70A/B2-287[»]
2IK2X-ray1.80A/B2-287[»]
2IK4X-ray1.80A/B2-287[»]
2IK6X-ray1.80A/B2-287[»]
2IK7X-ray1.90A/B2-287[»]
2IK9X-ray1.50A/B2-287[»]
8PRKX-ray1.85A/B1-287[»]
ProteinModelPortaliP00817.
SMRiP00817.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00817.

Family & Domainsi

Sequence similaritiesi

Belongs to the PPase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000017004.
HOGENOMiHOG000195569.
InParanoidiP00817.
KOiK01507.
OMAiANTLEHR.
OrthoDBiEOG092C3VG0.

Family and domain databases

CDDicd00412. pyrophosphatase. 1 hit.
Gene3Di3.90.80.10. 1 hit.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYTTRQIGA KNTLEYKVYI EKDGKPVSAF HDIPLYADKE NNIFNMVVEI
60 70 80 90 100
PRWTNAKLEI TKEETLNPII QDTKKGKLRF VRNCFPHHGY IHNYGAFPQT
110 120 130 140 150
WEDPNVSHPE TKAVGDNDPI DVLEIGETIA YTGQVKQVKA LGIMALLDEG
160 170 180 190 200
ETDWKVIAID INDPLAPKLN DIEDVEKYFP GLLRATNEWF RIYKIPDGKP
210 220 230 240 250
ENQFAFSGEA KNKKYALDII KETHDSWKQL IAGKSSDSKG IDLTNVTLPD
260 270 280
TPTYSKAASD AIPPASPKAD APIDKSIDKW FFISGSV
Length:287
Mass (Da):32,300
Last modified:July 24, 2007 - v4
Checksum:i1DC19A702A389BA9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41N → D AA sequence (PubMed:340461).Curated1
Sequence conflicti72D → N AA sequence (PubMed:340461).Curated1
Sequence conflicti75Missing AA sequence (PubMed:340461).Curated1
Sequence conflicti124E → Q AA sequence (PubMed:340461).Curated1
Sequence conflicti137Q → E AA sequence (PubMed:340461).Curated1
Sequence conflicti187N → D AA sequence (PubMed:340461).Curated1
Sequence conflicti225D → N AA sequence (PubMed:340461).Curated1
Sequence conflicti267P → L in CAA31629 (PubMed:2849749).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13253 Genomic DNA. Translation: CAA31629.1.
Z35880 Genomic DNA. Translation: CAA84949.1.
AY692953 Genomic DNA. Translation: AAT92972.1.
BK006936 Genomic DNA. Translation: DAA07132.1.
PIRiS45864. PWBY.
RefSeqiNP_009565.1. NM_001178359.1.

Genome annotation databases

EnsemblFungiiYBR011C; YBR011C; YBR011C.
GeneIDi852296.
KEGGisce:YBR011C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13253 Genomic DNA. Translation: CAA31629.1.
Z35880 Genomic DNA. Translation: CAA84949.1.
AY692953 Genomic DNA. Translation: AAT92972.1.
BK006936 Genomic DNA. Translation: DAA07132.1.
PIRiS45864. PWBY.
RefSeqiNP_009565.1. NM_001178359.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
117EX-ray2.15A/B2-287[»]
1E6AX-ray1.90A/B2-287[»]
1E9GX-ray1.15A/B2-287[»]
1HUJX-ray2.10A/B2-282[»]
1HUKX-ray2.20A/B2-282[»]
1M38X-ray1.80A/B1-287[»]
1PYPX-ray3.00A/B2-287[»]
1WGIX-ray2.20A/B2-287[»]
1WGJX-ray2.00A/B2-287[»]
1YPPX-ray2.40A/B2-287[»]
2IHPX-ray1.50A/B2-287[»]
2IK0X-ray1.70A/B2-287[»]
2IK1X-ray1.70A/B2-287[»]
2IK2X-ray1.80A/B2-287[»]
2IK4X-ray1.80A/B2-287[»]
2IK6X-ray1.80A/B2-287[»]
2IK7X-ray1.90A/B2-287[»]
2IK9X-ray1.50A/B2-287[»]
8PRKX-ray1.85A/B1-287[»]
ProteinModelPortaliP00817.
SMRiP00817.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32712. 84 interactors.
DIPiDIP-5753N.
IntActiP00817. 34 interactors.
MINTiMINT-614477.

PTM databases

iPTMnetiP00817.

2D gel databases

COMPLUYEAST-2DPAGEP00817.
SWISS-2DPAGEP00817.

Proteomic databases

MaxQBiP00817.
PRIDEiP00817.
TopDownProteomicsiP00817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR011C; YBR011C; YBR011C.
GeneIDi852296.
KEGGisce:YBR011C.

Organism-specific databases

EuPathDBiFungiDB:YBR011C.
SGDiS000000215. IPP1.

Phylogenomic databases

GeneTreeiENSGT00390000017004.
HOGENOMiHOG000195569.
InParanoidiP00817.
KOiK01507.
OMAiANTLEHR.
OrthoDBiEOG092C3VG0.

Enzyme and pathway databases

BioCyciYEAST:YBR011C-MONOMER.
BRENDAi3.6.1.1. 984.
ReactomeiR-SCE-379716. Cytosolic tRNA aminoacylation.
R-SCE-379726. Mitochondrial tRNA aminoacylation.
R-SCE-71737. Pyrophosphate hydrolysis.

Miscellaneous databases

EvolutionaryTraceiP00817.
PROiP00817.

Family and domain databases

CDDicd00412. pyrophosphatase. 1 hit.
Gene3Di3.90.80.10. 1 hit.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIPYR_YEAST
AccessioniPrimary (citable) accession number: P00817
Secondary accession number(s): D6VQ12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 24, 2007
Last modified: November 2, 2016
This is version 182 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 68400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.