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Protein

Cyanate hydratase

Gene

cynS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide.

Catalytic activityi

Cyanate + HCO3- + 2 H+ = NH3 + 2 CO2.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei961
Active sitei991
Active sitei1221

GO - Molecular functioni

  • cyanate hydratase activity Source: EcoCyc
  • DNA binding Source: InterPro

GO - Biological processi

  • cyanate catabolic process Source: EcoCyc

Keywordsi

Molecular functionLyase

Enzyme and pathway databases

BioCyciEcoCyc:CYANLY-MONOMER
MetaCyc:CYANLY-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Cyanate hydratase (EC:4.2.1.104)
Short name:
Cyanase
Alternative name(s):
Cyanate hydrolase
Cyanate lyase
Gene namesi
Name:cynS
Synonyms:cnt
Ordered Locus Names:b0340, JW0331
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG10175 cynS

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001875231 – 156Cyanate hydrataseAdd BLAST156

Proteomic databases

PaxDbiP00816
PRIDEiP00816

Interactioni

Subunit structurei

Homodecamer composed of five homodimers.

Protein-protein interaction databases

BioGridi4263187, 5 interactors
DIPiDIP-9365N
IntActiP00816, 3 interactors
STRINGi316385.ECDH10B_1353

Structurei

Secondary structure

1156
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 24Combined sources16
Helixi29 – 33Combined sources5
Beta strandi36 – 38Combined sources3
Helixi40 – 47Combined sources8
Helixi55 – 64Combined sources10
Helixi69 – 74Combined sources6
Beta strandi85 – 88Combined sources4
Helixi92 – 115Combined sources24
Beta strandi118 – 134Combined sources17
Beta strandi138 – 152Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DW9X-ray1.65A/B/C/D/E/F/G/H/I/J1-156[»]
1DWKX-ray1.65A/B/C/D/E/F/G/H/I/J1-156[»]
2IU7X-ray1.91A/B/C/D/E/F/G/H/I/J1-156[»]
2IUOX-ray1.90A/B/C/D/E/F/G/H/I/J1-156[»]
2IV1X-ray1.88A/B/C/D/E/F/G/H/I/J1-156[»]
2IVBX-ray1.95A/B/C/D/E/F/G/H/I/J1-156[»]
2IVGX-ray1.87A/B/C/D/E/F/G/H/I/J1-156[»]
2IVQX-ray2.10A/B/C/D/E/F/G/H/I/J1-156[»]
ProteinModelPortaliP00816
SMRiP00816
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00816

Family & Domainsi

Sequence similaritiesi

Belongs to the cyanase family.Curated

Phylogenomic databases

eggNOGiENOG4108V7B Bacteria
COG1513 LUCA
HOGENOMiHOG000043436
InParanoidiP00816
KOiK01725
OMAiITMSGKF
PhylomeDBiP00816

Family and domain databases

CDDicd00559 Cyanase_C, 1 hit
Gene3Di3.30.1160.10, 1 hit
HAMAPiMF_00535 Cyanate_hydrat, 1 hit
InterProiView protein in InterPro
IPR008076 Cyanase
IPR003712 Cyanate_lyase_C
IPR036581 Cyanate_lyase_C_sf
IPR010982 Lambda_DNA-bd_dom_sf
PANTHERiPTHR34186 PTHR34186, 1 hit
PfamiView protein in Pfam
PF02560 Cyanate_lyase, 1 hit
PIRSFiPIRSF001263 Cyanate_hydratas, 1 hit
PRINTSiPR01693 CYANASE
SMARTiView protein in SMART
SM01116 Cyanate_lyase, 1 hit
SUPFAMiSSF47413 SSF47413, 1 hit
SSF55234 SSF55234, 1 hit
TIGRFAMsiTIGR00673 cynS, 1 hit

Sequencei

Sequence statusi: Complete.

P00816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQSQINRNI RLDLADAILL SKAKKDLSFA EIADGTGLAE AFVTAALLGQ
60 70 80 90 100
QALPADAARL VGAKLDLDED SILLLQMIPL RGCIDDRIPT DPTMYRFYEM
110 120 130 140 150
LQVYGTTLKA LVHEKFGDGI ISAINFKLDV KKVADPEGGE RAVITLDGKY

LPTKPF
Length:156
Mass (Da):17,049
Last modified:July 1, 1989 - v2
Checksum:i13AC830926313CED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34D → N (PubMed:3049588).Curated1
Sequence conflicti68D → N (PubMed:3049588).Curated1
Sequence conflicti74L → S (PubMed:3049588).Curated1
Sequence conflicti125N → D (PubMed:3049588).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17891 Genomic DNA Translation: AAA23629.1
M23219 Genomic DNA Translation: AAA23626.1
U73857 Genomic DNA Translation: AAB18064.1
U00096 Genomic DNA Translation: AAC73443.1
AP009048 Genomic DNA Translation: BAE76122.1
PIRiA91850 YNEC
RefSeqiNP_414874.1, NC_000913.3
WP_000616243.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73443; AAC73443; b0340
BAE76122; BAE76122; BAE76122
GeneIDi948998
KEGGiecj:JW0331
eco:b0340
PATRICifig|1411691.4.peg.1937

Entry informationi

Entry nameiCYNS_ECOLI
AccessioniPrimary (citable) accession number: P00816
Secondary accession number(s): Q2MC84
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: March 28, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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