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Protein

Cyanate hydratase

Gene

cynS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide.

Catalytic activityi

Cyanate + HCO3- + 2 H+ = NH3 + 2 CO2.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei961
Active sitei991
Active sitei1221

GO - Molecular functioni

  • cyanate hydratase activity Source: EcoCyc
  • DNA binding Source: InterPro

GO - Biological processi

  • cyanate catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Enzyme and pathway databases

BioCyciEcoCyc:CYANLY-MONOMER.
ECOL316407:JW0331-MONOMER.
MetaCyc:CYANLY-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyanate hydratase (EC:4.2.1.104)
Short name:
Cyanase
Alternative name(s):
Cyanate hydrolase
Cyanate lyase
Gene namesi
Name:cynS
Synonyms:cnt
Ordered Locus Names:b0340, JW0331
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10175. cynS.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001875231 – 156Cyanate hydrataseAdd BLAST156

Proteomic databases

PaxDbiP00816.
PRIDEiP00816.

Interactioni

Subunit structurei

Homodecamer composed of five homodimers.

Protein-protein interaction databases

BioGridi4263187. 4 interactors.
DIPiDIP-9365N.
IntActiP00816. 3 interactors.
STRINGi511145.b0340.

Structurei

Secondary structure

1156
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 24Combined sources16
Helixi29 – 33Combined sources5
Beta strandi36 – 38Combined sources3
Helixi40 – 47Combined sources8
Helixi55 – 64Combined sources10
Helixi69 – 74Combined sources6
Beta strandi85 – 88Combined sources4
Helixi92 – 115Combined sources24
Beta strandi118 – 134Combined sources17
Beta strandi138 – 152Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DW9X-ray1.65A/B/C/D/E/F/G/H/I/J1-156[»]
1DWKX-ray1.65A/B/C/D/E/F/G/H/I/J1-156[»]
2IU7X-ray1.91A/B/C/D/E/F/G/H/I/J1-156[»]
2IUOX-ray1.90A/B/C/D/E/F/G/H/I/J1-156[»]
2IV1X-ray1.88A/B/C/D/E/F/G/H/I/J1-156[»]
2IVBX-ray1.95A/B/C/D/E/F/G/H/I/J1-156[»]
2IVGX-ray1.87A/B/C/D/E/F/G/H/I/J1-156[»]
2IVQX-ray2.10A/B/C/D/E/F/G/H/I/J1-156[»]
ProteinModelPortaliP00816.
SMRiP00816.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00816.

Family & Domainsi

Sequence similaritiesi

Belongs to the cyanase family.Curated

Phylogenomic databases

eggNOGiENOG4108V7B. Bacteria.
COG1513. LUCA.
HOGENOMiHOG000043436.
InParanoidiP00816.
KOiK01725.
OMAiPMKDVIQ.
PhylomeDBiP00816.

Family and domain databases

CDDicd00559. Cyanase_C. 1 hit.
Gene3Di1.10.260.40. 1 hit.
3.30.1160.10. 1 hit.
HAMAPiMF_00535. Cyanate_hydrat. 1 hit.
InterProiIPR008076. Cyanase.
IPR003712. Cyanate_lyase_C.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF02560. Cyanate_lyase. 1 hit.
[Graphical view]
PIRSFiPIRSF001263. Cyanate_hydratas. 1 hit.
PRINTSiPR01693. CYANASE.
SMARTiSM01116. Cyanate_lyase. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF55234. SSF55234. 1 hit.
TIGRFAMsiTIGR00673. cynS. 1 hit.

Sequencei

Sequence statusi: Complete.

P00816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQSQINRNI RLDLADAILL SKAKKDLSFA EIADGTGLAE AFVTAALLGQ
60 70 80 90 100
QALPADAARL VGAKLDLDED SILLLQMIPL RGCIDDRIPT DPTMYRFYEM
110 120 130 140 150
LQVYGTTLKA LVHEKFGDGI ISAINFKLDV KKVADPEGGE RAVITLDGKY

LPTKPF
Length:156
Mass (Da):17,049
Last modified:July 1, 1989 - v2
Checksum:i13AC830926313CED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34D → N (PubMed:3049588).Curated1
Sequence conflicti68D → N (PubMed:3049588).Curated1
Sequence conflicti74L → S (PubMed:3049588).Curated1
Sequence conflicti125N → D (PubMed:3049588).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17891 Genomic DNA. Translation: AAA23629.1.
M23219 Genomic DNA. Translation: AAA23626.1.
U73857 Genomic DNA. Translation: AAB18064.1.
U00096 Genomic DNA. Translation: AAC73443.1.
AP009048 Genomic DNA. Translation: BAE76122.1.
PIRiA91850. YNEC.
RefSeqiNP_414874.1. NC_000913.3.
WP_000616243.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73443; AAC73443; b0340.
BAE76122; BAE76122; BAE76122.
GeneIDi948998.
KEGGiecj:JW0331.
eco:b0340.
PATRICi32115813. VBIEscCol129921_0348.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17891 Genomic DNA. Translation: AAA23629.1.
M23219 Genomic DNA. Translation: AAA23626.1.
U73857 Genomic DNA. Translation: AAB18064.1.
U00096 Genomic DNA. Translation: AAC73443.1.
AP009048 Genomic DNA. Translation: BAE76122.1.
PIRiA91850. YNEC.
RefSeqiNP_414874.1. NC_000913.3.
WP_000616243.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DW9X-ray1.65A/B/C/D/E/F/G/H/I/J1-156[»]
1DWKX-ray1.65A/B/C/D/E/F/G/H/I/J1-156[»]
2IU7X-ray1.91A/B/C/D/E/F/G/H/I/J1-156[»]
2IUOX-ray1.90A/B/C/D/E/F/G/H/I/J1-156[»]
2IV1X-ray1.88A/B/C/D/E/F/G/H/I/J1-156[»]
2IVBX-ray1.95A/B/C/D/E/F/G/H/I/J1-156[»]
2IVGX-ray1.87A/B/C/D/E/F/G/H/I/J1-156[»]
2IVQX-ray2.10A/B/C/D/E/F/G/H/I/J1-156[»]
ProteinModelPortaliP00816.
SMRiP00816.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263187. 4 interactors.
DIPiDIP-9365N.
IntActiP00816. 3 interactors.
STRINGi511145.b0340.

Proteomic databases

PaxDbiP00816.
PRIDEiP00816.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73443; AAC73443; b0340.
BAE76122; BAE76122; BAE76122.
GeneIDi948998.
KEGGiecj:JW0331.
eco:b0340.
PATRICi32115813. VBIEscCol129921_0348.

Organism-specific databases

EchoBASEiEB0172.
EcoGeneiEG10175. cynS.

Phylogenomic databases

eggNOGiENOG4108V7B. Bacteria.
COG1513. LUCA.
HOGENOMiHOG000043436.
InParanoidiP00816.
KOiK01725.
OMAiPMKDVIQ.
PhylomeDBiP00816.

Enzyme and pathway databases

BioCyciEcoCyc:CYANLY-MONOMER.
ECOL316407:JW0331-MONOMER.
MetaCyc:CYANLY-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP00816.
PROiP00816.

Family and domain databases

CDDicd00559. Cyanase_C. 1 hit.
Gene3Di1.10.260.40. 1 hit.
3.30.1160.10. 1 hit.
HAMAPiMF_00535. Cyanate_hydrat. 1 hit.
InterProiIPR008076. Cyanase.
IPR003712. Cyanate_lyase_C.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF02560. Cyanate_lyase. 1 hit.
[Graphical view]
PIRSFiPIRSF001263. Cyanate_hydratas. 1 hit.
PRINTSiPR01693. CYANASE.
SMARTiSM01116. Cyanate_lyase. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF55234. SSF55234. 1 hit.
TIGRFAMsiTIGR00673. cynS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCYNS_ECOLI
AccessioniPrimary (citable) accession number: P00816
Secondary accession number(s): Q2MC84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.