Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arginase

Gene

CAR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 42800 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-arginine + H2O = L-ornithine + urea.By similarity

Cofactori

Mn2+PROSITE-ProRule annotationNote: Binds 2 manganese ions per subunit.PROSITE-ProRule annotation

Pathwayi: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and urea from L-arginine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Arginase (CAR1)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine and urea from L-arginine, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi123Manganese 1PROSITE-ProRule annotation1
Metal bindingi146Manganese 1PROSITE-ProRule annotation1
Metal bindingi146Manganese 2PROSITE-ProRule annotation1
Metal bindingi148Manganese 2PROSITE-ProRule annotation1
Metal bindingi150Manganese 1PROSITE-ProRule annotation1
Binding sitei205SubstrateBy similarity1
Metal bindingi256Manganese 1PROSITE-ProRule annotation1
Metal bindingi256Manganese 2PROSITE-ProRule annotation1
Metal bindingi258Manganese 2PROSITE-ProRule annotation1
Binding sitei270SubstrateBy similarity1
Binding sitei301SubstrateBy similarity1

GO - Molecular functioni

  • arginase activity Source: SGD
  • manganese ion binding Source: SGD
  • ornithine carbamoyltransferase inhibitor activity Source: SGD
  • zinc ion binding Source: SGD

GO - Biological processi

  • arginine catabolic process to ornithine Source: SGD
  • regulation of ornithine metabolic process Source: SGD
  • urea cycle Source: UniProtKB-UniPathway

Keywordsi

Molecular functionHydrolase
Biological processArginine metabolism
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YPL111W-MONOMER
YEAST:YPL111W-MONOMER
ReactomeiR-SCE-351143 Agmatine biosynthesis
R-SCE-6798695 Neutrophil degranulation
R-SCE-70635 Urea cycle
SABIO-RKiP00812
UniPathwayiUPA00158; UER00270

Protein family/group databases

MoonProtiP00812

Names & Taxonomyi

Protein namesi
Recommended name:
Arginase (EC:3.5.3.1By similarity)
Gene namesi
Name:CAR1
Ordered Locus Names:YPL111W
ORF Names:LPH15W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL111W
SGDiS000006032 CAR1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001737111 – 333ArginaseAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei16PhosphoserineCombined sources1
Modified residuei77PhosphothreonineCombined sources1
Modified residuei270PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP00812
PaxDbiP00812
PRIDEiP00812

2D gel databases

UCD-2DPAGEiP00812

PTM databases

iPTMnetiP00812

Expressioni

Inductioni

By arginine or homoarginine.

Interactioni

Subunit structurei

Homotrimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
ARG3P051504EBI-2856,EBI-12712

Protein-protein interaction databases

BioGridi36070, 58 interactors
DIPiDIP-1224N
IntActiP00812, 19 interactors
MINTiP00812
STRINGi4932.YPL111W

Structurei

3D structure databases

ProteinModelPortaliP00812
SMRiP00812
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 152Substrate bindingBy similarity5
Regioni159 – 161Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the arginase family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063082
HOGENOMiHOG000204319
InParanoidiP00812
KOiK01476
OMAiSAVARHC
OrthoDBiEOG092C3GMN

Family and domain databases

InterProiView protein in InterPro
IPR014033 Arginase
IPR006035 Ureohydrolase
IPR023696 Ureohydrolase_dom_sf
IPR020855 Ureohydrolase_Mn_BS
PfamiView protein in Pfam
PF00491 Arginase, 1 hit
PRINTSiPR00116 ARGINASE
SUPFAMiSSF52768 SSF52768, 1 hit
TIGRFAMsiTIGR01229 rocF_arginase, 1 hit
PROSITEiView protein in PROSITE
PS01053 ARGINASE_1, 1 hit
PS51409 ARGINASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P00812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METGPHYNYY KNRELSIVLA PFSGGQGKLG VEKGPKYMLK HGLQTSIEDL
60 70 80 90 100
GWSTELEPSM DEAQFVGKLK MEKDSTTGGS SVMIDGVKAK RADLVGEATK
110 120 130 140 150
LVYNSVSKVV QANRFPLTLG GDHSIAIGTV SAVLDKYPDA GLLWIDAHAD
160 170 180 190 200
INTIESTPSG NLHGCPVSFL MGLNKDVPHC PESLKWVPGN LSPKKIAYIG
210 220 230 240 250
LRDVDAGEKK ILKDLGIAAF SMYHVDKYGI NAVIEMAMKA VHPETNGEGP
260 270 280 290 300
IMCSYDVDGV DPLYIPATGT PVRGGLTLRE GLFLVERLAE SGNLIALDVV
310 320 330
ECNPDLAIHD IHVSNTISAG CAIARCALGE TLL
Length:333
Mass (Da):35,662
Last modified:July 21, 1986 - v1
Checksum:iA5979DC9F1FDFF2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10414 Genomic DNA Translation: AAA34470.1
U43503 Genomic DNA Translation: AAB68250.1
M10110 Genomic DNA Translation: AAA34469.1
BK006949 Genomic DNA Translation: DAA11322.1
PIRiA01008 WZBYR
RefSeqiNP_015214.1, NM_001183925.1

Genome annotation databases

EnsemblFungiiYPL111W; YPL111W; YPL111W
GeneIDi855993
KEGGisce:YPL111W

Similar proteinsi

Entry informationi

Entry nameiARGI_YEAST
AccessioniPrimary (citable) accession number: P00812
Secondary accession number(s): D6W3Q6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 23, 2018
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health