Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P00809

- BLAC_BACCE

UniProt

P00809 - BLAC_BACCE

Protein

Beta-lactamase 1

Gene

blaY

Organism
Bacillus cereus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This protein is a beta-lactamase with a substrate specificity for penicillins.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei91 – 911Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei187 – 1871Proton acceptorBy similarity

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Enzyme and pathway databases

    SABIO-RKP00809.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase 1 (EC:3.5.2.6)
    Alternative name(s):
    Beta-lactamase I
    Penicillinase
    Gene namesi
    Name:blaY
    OrganismiBacillus cereus
    Taxonomic identifieri1396 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Add
    BLAST
    Chaini28 – 306279Beta-lactamase 1PRO_0000016970Add
    BLAST

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CN9model-A80-104[»]
    A258-270[»]
    ProteinModelPortaliP00809.
    SMRiP00809. Positions 54-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni253 – 2553Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PfamiPF00144. Beta-lactamase. 1 hit.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00809-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILKNKRMLK IGICVGILGL SITSLEAFTG ESLQVEAKEK TGQVKHKNQA    50
    THKEFSQLEK KFDARLGVYA IDTGTNQTIS YRPNERFAFA STYKALAAGV 100
    LLQQNSIDSL NEVITYTKED LVDYSPVTEK HVDTGMKLGE IAEAAVRSSD 150
    NTAGNILFNK IGGPKGYEKA LRHMGDRITM SNRFETELNE AIPGDIRDTS 200
    TAKAIATNLK AFTVGNALPA EKRKILTEWM KGNATGDKLI RAGIPTDWVV 250
    GDKSGAGSYG TRNDIAVVWP PNSAPIIVLI SSKDEKEAIY NDQLIAEATK 300
    VIVKGS 306
    Length:306
    Mass (Da):33,322
    Last modified:July 21, 1986 - v1
    Checksum:iF00F4DB9DB6D41AA
    GO

    Sequence cautioni

    The sequence CAA26021.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA29819.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti273 – 2731S → R in CAA29819. (PubMed:3124817)Curated
    Sequence conflicti278 – 2803VLI → IAIL in CAA29819. (PubMed:3124817)Curated
    Sequence conflicti291 – 2922ND → DN in CAA29819. (PubMed:3124817)Curated
    Sequence conflicti305 – 3062GS → ALR in CAA29819. (PubMed:3124817)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01990 Genomic DNA. Translation: CAA26021.1. Different initiation.
    X01602 Genomic DNA. Translation: CAA25753.1.
    X06599 Genomic DNA. Translation: CAA29819.1. Different initiation.
    PIRiA01004. PNBSU.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01990 Genomic DNA. Translation: CAA26021.1 . Different initiation.
    X01602 Genomic DNA. Translation: CAA25753.1 .
    X06599 Genomic DNA. Translation: CAA29819.1 . Different initiation.
    PIRi A01004. PNBSU.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CN9 model - A 80-104 [» ]
    A 258-270 [» ]
    ProteinModelPortali P00809.
    SMRi P00809. Positions 54-305.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL5732.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK P00809.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    Pfami PF00144. Beta-lactamase. 1 hit.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequence of the type I beta-lactamase gene from Bacillus cereus."
      Sloma A., Gross M.
      Nucleic Acids Res. 11:4997-5004(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 569/H / NCTC 9945.
    2. "Beta-lactamase I from Bacillus cereus. Structure and site-directed mutagenesis."
      Madgwick P.J., Waley S.G.
      Biochem. J. 248:657-662(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 569/H/9.
    3. "Bacillus cereus 569/H beta-lactamase I: cloning in Escherichia coli and signal sequence determination."
      Mezes P.S.F., Yang Y.Q., Hussain M., Lampen J.O.
      FEBS Lett. 161:195-200(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-75.
      Strain: 569/H / NCTC 9945.

    Entry informationi

    Entry nameiBLAC_BACCE
    AccessioniPrimary (citable) accession number: P00809
    Secondary accession number(s): P70876
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3