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P00808

- BLAC_BACLI

UniProt

P00808 - BLAC_BACLI

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Protein
Beta-lactamase
Gene
penP, blaP
Organism
Bacillus licheniformis
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei86 – 861Acyl-ester intermediate
Active sitei182 – 1821Proton acceptor

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Cleaved into the following 2 chains:
Gene namesi
Name:penP
Synonyms:blaP
OrganismiBacillus licheniformis
Taxonomic identifieri1402 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626
Add
BLAST
Chaini27 – 307281Large exopenicillinase
PRO_0000016973Add
BLAST
Chaini43 – 307265Small exopenicillinase
PRO_0000016974Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi27 – 271N-palmitoyl cysteine By similarity
Lipidationi27 – 271S-diacylglycerol cysteine By similarity

Post-translational modificationi

Large exopenicillinase is the primary secretion product; it can be converted to small exopenicillinase.

Keywords - PTMi

Lipoprotein, Palmitate

Expressioni

Inductioni

By the bacillus licheniformis protein BlaR1.

Interactioni

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi49 – 579
Beta strandi60 – 678
Turni68 – 703
Beta strandi73 – 775
Beta strandi81 – 833
Helixi85 – 873
Helixi88 – 9912
Helixi103 – 1064
Turni113 – 1153
Helixi123 – 1253
Turni127 – 1293
Helixi133 – 14311
Helixi146 – 15510
Helixi158 – 16811
Beta strandi178 – 1803
Helixi182 – 1843
Beta strandi194 – 1963
Helixi197 – 20812
Beta strandi210 – 2134
Helixi215 – 22612
Turni232 – 2343
Helixi235 – 2384
Beta strandi244 – 2529
Turni253 – 2553
Beta strandi256 – 2649
Beta strandi266 – 2683
Beta strandi271 – 2788
Helixi289 – 30113

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I2SX-ray1.70A/B26-307[»]
1I2WX-ray1.70A/B26-307[»]
1MBLX-ray2.00A/B48-303[»]
1W7FX-ray1.80A/B1-307[»]
2BLMX-ray2.00A/B43-307[»]
2WK0X-ray1.65A/B43-307[»]
2Y91X-ray2.00A/B43-307[»]
3B3XX-ray2.50A/B43-307[»]
3LY3X-ray1.80A47-305[»]
3LY4X-ray1.80A47-303[»]
3M2JX-ray1.80A/B47-303[»]
3M2KX-ray3.50A/B47-303[»]
3SH7X-ray2.50A/B43-307[»]
3SH8X-ray2.00A/B43-307[»]
3SH9X-ray1.90A/B43-307[»]
3SOIX-ray1.73A/B46-303[»]
4BLMX-ray2.00A/B43-307[»]
ProteinModelPortaliP00808.
SMRiP00808. Positions 48-303.

Miscellaneous databases

EvolutionaryTraceiP00808.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni248 – 2503Substrate binding

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00808-1 [UniParc]FASTAAdd to Basket

« Hide

MKLWFSTLKL KKAAAVLLFS CVALAGCANN QTNASQPAEK NEKTEMKDDF    50
AKLEEQFDAK LGIFALDTGT NRTVAYRPDE RFAFASTIKA LTVGVLLQQK 100
SIEDLNQRIT YTRDDLVNYN PITEKHVDTG MTLKELADAS LRYSDNAAQN 150
LILKQIGGPE SLKKELRKIG DEVTNPERFE PELNEVNPGE TQDTSTARAL 200
VTSLRAFALE DKLPSEKREL LIDWMKRNTT GDALIRAGVP DGWEVADKTG 250
AASYGTRNDI AIIWPPKGDP VVLAVLSSRD KKDAKYDDKL IAEATKVVMK 300
ALNMNGK 307
Length:307
Mass (Da):33,996
Last modified:July 21, 1986 - v1
Checksum:i7E25B1E72129A6DA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V00093 Genomic DNA. Translation: CAA23431.1.
M21337 Genomic DNA. Translation: AAA22649.2.
PIRiA93727. PNBSL.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V00093 Genomic DNA. Translation: CAA23431.1 .
M21337 Genomic DNA. Translation: AAA22649.2 .
PIRi A93727. PNBSL.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1I2S X-ray 1.70 A/B 26-307 [» ]
1I2W X-ray 1.70 A/B 26-307 [» ]
1MBL X-ray 2.00 A/B 48-303 [» ]
1W7F X-ray 1.80 A/B 1-307 [» ]
2BLM X-ray 2.00 A/B 43-307 [» ]
2WK0 X-ray 1.65 A/B 43-307 [» ]
2Y91 X-ray 2.00 A/B 43-307 [» ]
3B3X X-ray 2.50 A/B 43-307 [» ]
3LY3 X-ray 1.80 A 47-305 [» ]
3LY4 X-ray 1.80 A 47-303 [» ]
3M2J X-ray 1.80 A/B 47-303 [» ]
3M2K X-ray 3.50 A/B 47-303 [» ]
3SH7 X-ray 2.50 A/B 43-307 [» ]
3SH8 X-ray 2.00 A/B 43-307 [» ]
3SH9 X-ray 1.90 A/B 43-307 [» ]
3SOI X-ray 1.73 A/B 46-303 [» ]
4BLM X-ray 2.00 A/B 43-307 [» ]
ProteinModelPortali P00808.
SMRi P00808. Positions 48-303.
ModBasei Search...

Chemistry

BindingDBi P00808.
ChEMBLi CHEMBL5633.
DrugBanki DB01331. Cefoxitin.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P00808.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
Pfami PF00144. Beta-lactamase. 1 hit.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium."
    Neugebauer K., Sprengel R., Schaller H.
    Nucleic Acids Res. 9:2577-2588(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25972 / 749/C / DSM 8782 / NCIMB 11109 / IMET 10723.
  2. "The promoter-proximal region of the Bacillus licheniformis penicillinase gene: nucleotide sequence and predicted leader peptide sequence."
    Kroyer J., Chang S.
    Gene 15:343-347(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72.
    Strain: ATCC 25972 / 749/C / DSM 8782 / NCIMB 11109 / IMET 10723.
  3. "The amino acid sequence of penicillinase from Bacillus licheniformis."
    Meadway R.J.
    Biochem. J. 115:12P-13P(1969) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 43-307.
  4. "Regulation of the penicillinase genes of Bacillus licheniformis: interaction of the pen repressor with its operators."
    Wittman V., Wong H.C.
    J. Bacteriol. 170:3206-3212(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15.
    Strain: ATCC 25972 / 749/C / DSM 8782 / NCIMB 11109 / IMET 10723.
  5. "Large exopenicillinase, initial extracellular form detected in cultures of Bacillus licheniformis."
    Izui K., Nielsen J.B.K., Caulfield M.P., Lampen J.O.
    Biochemistry 19:1882-1886(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: CONFIRMATION OF THE AMINO ENDS OF THE LARGE AND SMALL EXOPENICILLINASE.
  6. "Beta-lactamase of Bacillus licheniformis 749/C at 2-A resolution."
    Moews P.C., Knox J.R., Dideberg O., Charlier P., Frere J.-M.
    Proteins 7:156-171(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
  7. "Beta-lactamase of Bacillus licheniformis 749/C. Refinement at 2-A resolution and analysis of hydration."
    Knox J.R., Moews P.C.
    J. Mol. Biol. 220:435-455(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
  8. "Crystal structures of the Bacillus licheniformis BS3 class A beta-lactamase and of the acyl-enzyme adduct formed with cefoxitin."
    Fonze E., Vanhove M., Dive G., Sauvage E., Frere J.-M., Charlier P.
    Biochemistry 41:1877-1885(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Entry informationi

Entry nameiBLAC_BACLI
AccessioniPrimary (citable) accession number: P00808
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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