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Protein

Beta-lactamase

Gene

penP

Organism
Bacillus licheniformis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Acyl-ester intermediate1
Active sitei182Proton acceptor1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BRENDAi3.5.2.6. 669.
SABIO-RKP00808.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Cleaved into the following 2 chains:
Gene namesi
Name:penP
Synonyms:blaP
OrganismiBacillus licheniformis
Taxonomic identifieri1402 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5633.
DrugBankiDB01331. Cefoxitin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Add BLAST26
ChainiPRO_000001697327 – 307Large exopenicillinaseAdd BLAST281
ChainiPRO_000001697443 – 307Small exopenicillinaseAdd BLAST265

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi27N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi27S-diacylglycerol cysteinePROSITE-ProRule annotation1

Post-translational modificationi

Large exopenicillinase is the primary secretion product; it can be converted to small exopenicillinase.

Keywords - PTMi

Lipoprotein, Palmitate

Expressioni

Inductioni

By the bacillus licheniformis protein BlaR1.

Interactioni

Chemistry databases

BindingDBiP00808.

Structurei

Secondary structure

1307
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi47 – 56Combined sources10
Beta strandi60 – 67Combined sources8
Turni68 – 70Combined sources3
Beta strandi73 – 77Combined sources5
Beta strandi81 – 83Combined sources3
Helixi85 – 87Combined sources3
Helixi88 – 99Combined sources12
Helixi103 – 106Combined sources4
Beta strandi108 – 110Combined sources3
Helixi113 – 115Combined sources3
Helixi123 – 125Combined sources3
Turni127 – 129Combined sources3
Helixi133 – 143Combined sources11
Helixi146 – 155Combined sources10
Helixi159 – 168Combined sources10
Beta strandi178 – 180Combined sources3
Helixi182 – 184Combined sources3
Beta strandi194 – 196Combined sources3
Helixi197 – 208Combined sources12
Beta strandi210 – 213Combined sources4
Helixi215 – 226Combined sources12
Turni232 – 234Combined sources3
Helixi235 – 238Combined sources4
Beta strandi244 – 252Combined sources9
Turni253 – 255Combined sources3
Beta strandi256 – 264Combined sources9
Beta strandi266 – 268Combined sources3
Beta strandi271 – 278Combined sources8
Helixi288 – 302Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I2SX-ray1.70A/B26-307[»]
1I2WX-ray1.70A/B26-307[»]
1MBLX-ray2.00A/B48-303[»]
1W7FX-ray1.80A/B1-307[»]
2BLMX-ray2.00A/B43-307[»]
2WK0X-ray1.65A/B43-307[»]
2Y91X-ray2.00A/B43-307[»]
3B3XX-ray2.50A/B43-307[»]
3LY3X-ray1.80A47-305[»]
3LY4X-ray1.80A47-303[»]
3M2JX-ray1.80A/B47-303[»]
3M2KX-ray3.50A/B47-303[»]
3SH7X-ray2.50A/B43-307[»]
3SH8X-ray2.00A/B43-307[»]
3SH9X-ray1.90A/B43-307[»]
3SOIX-ray1.73A/B46-303[»]
4BLMX-ray2.00A/B43-307[»]
4M3KX-ray1.48A44-307[»]
4N1HX-ray3.00A/C44-307[»]
4N92X-ray1.93A/B43-307[»]
4N9KX-ray1.93A/B43-307[»]
4N9LX-ray2.30A/B43-307[»]
ProteinModelPortaliP00808.
SMRiP00808.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00808.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni248 – 250Substrate binding3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00808-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLWFSTLKL KKAAAVLLFS CVALAGCANN QTNASQPAEK NEKTEMKDDF
60 70 80 90 100
AKLEEQFDAK LGIFALDTGT NRTVAYRPDE RFAFASTIKA LTVGVLLQQK
110 120 130 140 150
SIEDLNQRIT YTRDDLVNYN PITEKHVDTG MTLKELADAS LRYSDNAAQN
160 170 180 190 200
LILKQIGGPE SLKKELRKIG DEVTNPERFE PELNEVNPGE TQDTSTARAL
210 220 230 240 250
VTSLRAFALE DKLPSEKREL LIDWMKRNTT GDALIRAGVP DGWEVADKTG
260 270 280 290 300
AASYGTRNDI AIIWPPKGDP VVLAVLSSRD KKDAKYDDKL IAEATKVVMK

ALNMNGK
Length:307
Mass (Da):33,996
Last modified:July 21, 1986 - v1
Checksum:i7E25B1E72129A6DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00093 Genomic DNA. Translation: CAA23431.1.
M21337 Genomic DNA. Translation: AAA22649.2.
PIRiA93727. PNBSL.
RefSeqiWP_023857755.1. NZ_KN125579.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00093 Genomic DNA. Translation: CAA23431.1.
M21337 Genomic DNA. Translation: AAA22649.2.
PIRiA93727. PNBSL.
RefSeqiWP_023857755.1. NZ_KN125579.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I2SX-ray1.70A/B26-307[»]
1I2WX-ray1.70A/B26-307[»]
1MBLX-ray2.00A/B48-303[»]
1W7FX-ray1.80A/B1-307[»]
2BLMX-ray2.00A/B43-307[»]
2WK0X-ray1.65A/B43-307[»]
2Y91X-ray2.00A/B43-307[»]
3B3XX-ray2.50A/B43-307[»]
3LY3X-ray1.80A47-305[»]
3LY4X-ray1.80A47-303[»]
3M2JX-ray1.80A/B47-303[»]
3M2KX-ray3.50A/B47-303[»]
3SH7X-ray2.50A/B43-307[»]
3SH8X-ray2.00A/B43-307[»]
3SH9X-ray1.90A/B43-307[»]
3SOIX-ray1.73A/B46-303[»]
4BLMX-ray2.00A/B43-307[»]
4M3KX-ray1.48A44-307[»]
4N1HX-ray3.00A/C44-307[»]
4N92X-ray1.93A/B43-307[»]
4N9KX-ray1.93A/B43-307[»]
4N9LX-ray2.30A/B43-307[»]
ProteinModelPortaliP00808.
SMRiP00808.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP00808.
ChEMBLiCHEMBL5633.
DrugBankiDB01331. Cefoxitin.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.5.2.6. 669.
SABIO-RKP00808.

Miscellaneous databases

EvolutionaryTraceiP00808.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLAC_BACLI
AccessioniPrimary (citable) accession number: P00808
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.