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P00807 (BLAC_STAAU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase

EC=3.5.2.6
Alternative name(s):
Penicillinase
Gene names
Name:blaZ
Encoded onPlasmid pI258 Ref.1 Ref.4
OrganismStaphylococcus aureus
Taxonomic identifier1280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length281 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   Technical term3D-structure
Direct protein sequencing
Plasmid
Transposable element
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.6
Chain25 – 281257Beta-lactamase
PRO_0000017020

Regions

Region225 – 2273Substrate binding

Sites

Active site631Acyl-ester intermediate

Secondary structure

.................................................. 281
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00807 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: F82A836773C275FE

FASTA28131,349
        10         20         30         40         50         60 
MKKLIFLIVI ALVLSACNSN SSHAKELNDL EKKYNAHIGV YALDTKSGKE VKFNSDKRFA 

        70         80         90        100        110        120 
YASTSKAINS AILLEQVPYN KLNKKVHINK DDIVAYSPIL EKYVGKDITL KALIEASMTY 

       130        140        150        160        170        180 
SDNTANNKII KEIGGIKKVK QRLKELGDKV TNPVRYEIEL NYYSPKSKKD TSTPAAFGKT 

       190        200        210        220        230        240 
LNKLIANGKL SKENKKFLLD LMLNNKSGDT LIKDGVPKDY KVADKSGQAI TYASRNDVAF 

       250        260        270        280 
VYPKGQSEPI VLVIFTNKDN KSDKPNDKLI SETAKSVMKE F 

« Hide

References

[1]"Nucleotide sequence of the Staphylococcus aureus PC1 beta-lactamase gene."
Chan P.T.
Nucleic Acids Res. 14:5940-5940(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: PC-1.
[2]"Nucleotide sequence of the blaZ gene of the Staphylococcus aureus beta-lactamase transposon Tn4002."
Gillspie M.T., Skurray R.A.
Nucleic Acids Res. 17:8854-8854(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SK456.
Transposon: Tn4002.
[3]"Tn552, a novel transposable element from Staphylococcus aureus."
Rowland S.J., Dyke K.G.H.
Mol. Microbiol. 4:961-975(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NCTC 9789.
Transposon: Tn552.
[4]"Nucleotide sequence and expression of the beta-lactamase gene from Staphylococcus aureus plasmid pI258 in Escherichia coli, Bacillus subtilis, and Staphylococcus aureus."
Wang P.-Z., Novick R.P.
J. Bacteriol. 169:1763-1766(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Unique features in the ribosome binding site sequence of the Gram-positive Staphylococcus aureus beta-lactamase gene."
McLaughlin J.R., Murray C.L., Rabinowitz J.C.
J. Biol. Chem. 256:11283-11291(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-47.
[6]"The amino acid sequence of Staphylococcus aureus penicillinase."
Ambler R.P.
Biochem. J. 151:197-218(1975) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-281.
[7]"Bacterial resistance to beta-lactam antibiotics: crystal structure of beta-lactamase from Staphylococcus aureus PC1 at 2.5-A resolution."
Herzberg O., Moult J.
Science 236:694-701(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[8]"Refined crystal structure of beta-lactamase from Staphylococcus aureus PC1 at 2.0-A resolution."
Herzberg O.
J. Mol. Biol. 217:701-719(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[9]"Role of the omega-loop in the activity, substrate specificity, and structure of class A beta-lactamase."
Banerjee S., Pieper U., Kapadia G., Pannell L.K., Herzberg O.
Biochemistry 37:3286-3296(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[10]"Relocation of the catalytic carboxylate group in class A beta-lactamase: the structure and function of the mutant enzyme Glu166-->Gln:Asn170-->Asp."
Chen C.C., Herzberg O.
Protein Eng. 12:573-579(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04121 Genomic DNA. Translation: CAA27733.1.
X16471 Genomic DNA. Translation: CAA34491.1.
M15526 Genomic DNA. Translation: AAA98239.1.
X52734 Genomic DNA. Translation: CAA36953.1.
PIRPNSAP. A01002.
RefSeqNP_878023.1. NC_005054.1.
YP_003329488.1. NC_013550.1.
YP_006937602.1. NC_013319.1.
YP_006937751.1. NC_013323.1.
YP_006938263.1. NC_013337.1.
YP_006938770.1. NC_013352.1.
YP_008709799.1. NC_022598.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ALQX-ray1.80A24-281[»]
1BLCX-ray2.20A25-281[»]
1BLHX-ray2.30A25-281[»]
1BLPX-ray2.30A25-281[»]
1DJAX-ray1.90A25-281[»]
1DJBX-ray2.10A25-281[»]
1DJCX-ray2.00A25-281[»]
1GHIX-ray2.30A25-281[»]
1GHMX-ray1.86A25-281[»]
1GHPX-ray1.76A25-281[»]
1KGEX-ray2.00A25-281[»]
1KGFX-ray2.20A25-281[»]
1KGGX-ray2.30A25-281[»]
1OMEX-ray2.30A/B25-281[»]
1PIOX-ray2.80A/B25-281[»]
3BLMX-ray2.00A25-281[»]
ProteinModelPortalP00807.
SMRP00807. Positions 25-281.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP00807.
ChEMBLCHEMBL4114.
DrugBankDB00766. Clavulanate.
DB01598. Imipenem.

Proteomic databases

PRIDEP00807.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID13874750.
13874903.
13875430.
13875951.
17363239.
2598287.
8655740.

Phylogenomic databases

ProtClustDBCLSK888312.

Enzyme and pathway databases

SABIO-RKP00807.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00807.

Entry information

Entry nameBLAC_STAAU
AccessionPrimary (citable) accession number: P00807
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 19, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references