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P00807

- BLAC_STAAU

UniProt

P00807 - BLAC_STAAU

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Protein

Beta-lactamase

Gene

blaZ

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei63 – 631Acyl-ester intermediate

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

SABIO-RKP00807.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:blaZ
Encoded oniPlasmid pI2582 Publications
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationPROSITE-ProRule annotationAdd
BLAST
Chaini25 – 281257Beta-lactamasePRO_0000017020Add
BLAST

Proteomic databases

PRIDEiP00807.

Interactioni

Structurei

Secondary structure

1
281
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi26 – 338
Beta strandi37 – 448
Turni45 – 473
Beta strandi50 – 545
Helixi62 – 643
Helixi65 – 7612
Helixi79 – 835
Beta strandi85 – 884
Helixi90 – 923
Helixi100 – 1034
Beta strandi106 – 1094
Helixi110 – 12011
Helixi123 – 13311
Helixi136 – 14510
Helixi159 – 1613
Beta strandi170 – 1723
Helixi174 – 18411
Beta strandi187 – 1904
Helixi192 – 20413
Helixi206 – 2083
Turni209 – 2113
Helixi212 – 2154
Beta strandi220 – 2289
Beta strandi230 – 2323
Beta strandi235 – 2428
Beta strandi250 – 2578
Helixi267 – 27812

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ALQX-ray1.80A24-244[»]
1BLCX-ray2.20A25-281[»]
1BLHX-ray2.30A25-281[»]
1BLPX-ray2.30A25-281[»]
1DJAX-ray1.90A25-281[»]
1DJBX-ray2.10A25-281[»]
1DJCX-ray2.00A25-281[»]
1GHIX-ray2.30A25-281[»]
1GHMX-ray1.86A25-281[»]
1GHPX-ray1.76A25-281[»]
1KGEX-ray2.00A25-281[»]
1KGFX-ray2.20A25-281[»]
1KGGX-ray2.30A24-281[»]
1OMEX-ray2.30A/B25-281[»]
1PIOX-ray2.80A/B25-281[»]
3BLMX-ray2.00A25-281[»]
ProteinModelPortaliP00807.
SMRiP00807. Positions 25-281.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00807.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni225 – 2273Substrate binding

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00807-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKLIFLIVI ALVLSACNSN SSHAKELNDL EKKYNAHIGV YALDTKSGKE
60 70 80 90 100
VKFNSDKRFA YASTSKAINS AILLEQVPYN KLNKKVHINK DDIVAYSPIL
110 120 130 140 150
EKYVGKDITL KALIEASMTY SDNTANNKII KEIGGIKKVK QRLKELGDKV
160 170 180 190 200
TNPVRYEIEL NYYSPKSKKD TSTPAAFGKT LNKLIANGKL SKENKKFLLD
210 220 230 240 250
LMLNNKSGDT LIKDGVPKDY KVADKSGQAI TYASRNDVAF VYPKGQSEPI
260 270 280
VLVIFTNKDN KSDKPNDKLI SETAKSVMKE F
Length:281
Mass (Da):31,349
Last modified:July 21, 1986 - v1
Checksum:iF82A836773C275FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04121 Genomic DNA. Translation: CAA27733.1.
X16471 Genomic DNA. Translation: CAA34491.1.
M15526 Genomic DNA. Translation: AAA98239.1.
X52734 Genomic DNA. Translation: CAA36953.1.
PIRiA01002. PNSAP.
RefSeqiNP_878023.1. NC_005054.1.
YP_003329488.1. NC_013550.1.
YP_006937602.1. NC_013319.1.
YP_006937751.1. NC_013323.1.
YP_006938263.1. NC_013337.1.
YP_006938770.1. NC_013352.1.
YP_008709799.1. NC_022598.1.

Genome annotation databases

GeneIDi13874750.
13874903.
13875430.
13875951.
17363239.
2598287.
8655740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04121 Genomic DNA. Translation: CAA27733.1 .
X16471 Genomic DNA. Translation: CAA34491.1 .
M15526 Genomic DNA. Translation: AAA98239.1 .
X52734 Genomic DNA. Translation: CAA36953.1 .
PIRi A01002. PNSAP.
RefSeqi NP_878023.1. NC_005054.1.
YP_003329488.1. NC_013550.1.
YP_006937602.1. NC_013319.1.
YP_006937751.1. NC_013323.1.
YP_006938263.1. NC_013337.1.
YP_006938770.1. NC_013352.1.
YP_008709799.1. NC_022598.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ALQ X-ray 1.80 A 24-244 [» ]
1BLC X-ray 2.20 A 25-281 [» ]
1BLH X-ray 2.30 A 25-281 [» ]
1BLP X-ray 2.30 A 25-281 [» ]
1DJA X-ray 1.90 A 25-281 [» ]
1DJB X-ray 2.10 A 25-281 [» ]
1DJC X-ray 2.00 A 25-281 [» ]
1GHI X-ray 2.30 A 25-281 [» ]
1GHM X-ray 1.86 A 25-281 [» ]
1GHP X-ray 1.76 A 25-281 [» ]
1KGE X-ray 2.00 A 25-281 [» ]
1KGF X-ray 2.20 A 25-281 [» ]
1KGG X-ray 2.30 A 24-281 [» ]
1OME X-ray 2.30 A/B 25-281 [» ]
1PIO X-ray 2.80 A/B 25-281 [» ]
3BLM X-ray 2.00 A 25-281 [» ]
ProteinModelPortali P00807.
SMRi P00807. Positions 25-281.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P00807.
ChEMBLi CHEMBL4114.
DrugBanki DB00766. Clavulanate.
DB01598. Imipenem.

Proteomic databases

PRIDEi P00807.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13874750.
13874903.
13875430.
13875951.
17363239.
2598287.
8655740.

Enzyme and pathway databases

SABIO-RK P00807.

Miscellaneous databases

EvolutionaryTracei P00807.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
Pfami PF00144. Beta-lactamase. 1 hit.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the Staphylococcus aureus PC1 beta-lactamase gene."
    Chan P.T.
    Nucleic Acids Res. 14:5940-5940(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: PC-1.
    Plasmid: pI258
  2. "Nucleotide sequence of the blaZ gene of the Staphylococcus aureus beta-lactamase transposon Tn4002."
    Gillspie M.T., Skurray R.A.
    Nucleic Acids Res. 17:8854-8854(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SK456.
    Transposon: Tn4002.
  3. "Tn552, a novel transposable element from Staphylococcus aureus."
    Rowland S.J., Dyke K.G.H.
    Mol. Microbiol. 4:961-975(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NCTC 9789.
    Transposon: Tn552.
  4. "Nucleotide sequence and expression of the beta-lactamase gene from Staphylococcus aureus plasmid pI258 in Escherichia coli, Bacillus subtilis, and Staphylococcus aureus."
    Wang P.-Z., Novick R.P.
    J. Bacteriol. 169:1763-1766(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pI258
  5. "Unique features in the ribosome binding site sequence of the Gram-positive Staphylococcus aureus beta-lactamase gene."
    McLaughlin J.R., Murray C.L., Rabinowitz J.C.
    J. Biol. Chem. 256:11283-11291(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-47.
  6. "The amino acid sequence of Staphylococcus aureus penicillinase."
    Ambler R.P.
    Biochem. J. 151:197-218(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-281.
  7. "Bacterial resistance to beta-lactam antibiotics: crystal structure of beta-lactamase from Staphylococcus aureus PC1 at 2.5-A resolution."
    Herzberg O., Moult J.
    Science 236:694-701(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
  8. "Refined crystal structure of beta-lactamase from Staphylococcus aureus PC1 at 2.0-A resolution."
    Herzberg O.
    J. Mol. Biol. 217:701-719(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
  9. "Role of the omega-loop in the activity, substrate specificity, and structure of class A beta-lactamase."
    Banerjee S., Pieper U., Kapadia G., Pannell L.K., Herzberg O.
    Biochemistry 37:3286-3296(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
  10. "Relocation of the catalytic carboxylate group in class A beta-lactamase: the structure and function of the mutant enzyme Glu166-->Gln:Asn170-->Asp."
    Chen C.C., Herzberg O.
    Protein Eng. 12:573-579(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

Entry informationi

Entry nameiBLAC_STAAU
AccessioniPrimary (citable) accession number: P00807
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 1, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3