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Protein

Beta-lactamase

Gene

blaZ

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63Acyl-ester intermediate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

SABIO-RKP00807.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:blaZ
Encoded oniPlasmid pI2582 Publications
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4114.
DrugBankiDB00766. Clavulanate.
DB01598. Imipenem.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24PROSITE-ProRule annotation1 PublicationAdd BLAST24
ChainiPRO_000001702025 – 281Beta-lactamaseAdd BLAST257

Proteomic databases

PRIDEiP00807.

Interactioni

Chemistry databases

BindingDBiP00807.

Structurei

Secondary structure

1281
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi26 – 33Combined sources8
Beta strandi37 – 44Combined sources8
Turni45 – 47Combined sources3
Beta strandi50 – 54Combined sources5
Helixi62 – 64Combined sources3
Helixi65 – 76Combined sources12
Helixi79 – 83Combined sources5
Beta strandi85 – 88Combined sources4
Helixi90 – 92Combined sources3
Helixi100 – 103Combined sources4
Beta strandi106 – 109Combined sources4
Helixi110 – 120Combined sources11
Helixi123 – 133Combined sources11
Helixi136 – 145Combined sources10
Helixi159 – 161Combined sources3
Beta strandi170 – 172Combined sources3
Helixi174 – 184Combined sources11
Beta strandi187 – 190Combined sources4
Helixi192 – 204Combined sources13
Helixi206 – 208Combined sources3
Turni209 – 211Combined sources3
Helixi212 – 215Combined sources4
Beta strandi220 – 228Combined sources9
Beta strandi230 – 232Combined sources3
Beta strandi235 – 242Combined sources8
Beta strandi250 – 257Combined sources8
Helixi267 – 278Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ALQX-ray1.80A24-244[»]
1BLCX-ray2.20A25-281[»]
1BLHX-ray2.30A25-281[»]
1BLPX-ray2.30A25-281[»]
1DJAX-ray1.90A25-281[»]
1DJBX-ray2.10A25-281[»]
1DJCX-ray2.00A25-281[»]
1GHIX-ray2.30A25-281[»]
1GHMX-ray1.86A25-281[»]
1GHPX-ray1.76A25-281[»]
1KGEX-ray2.00A25-281[»]
1KGFX-ray2.20A25-281[»]
1KGGX-ray2.30A24-281[»]
1OMEX-ray2.30A/B25-281[»]
1PIOX-ray2.80A/B25-281[»]
3BLMX-ray2.00A25-281[»]
ProteinModelPortaliP00807.
SMRiP00807.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00807.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni225 – 227Substrate binding3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00807-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLIFLIVI ALVLSACNSN SSHAKELNDL EKKYNAHIGV YALDTKSGKE
60 70 80 90 100
VKFNSDKRFA YASTSKAINS AILLEQVPYN KLNKKVHINK DDIVAYSPIL
110 120 130 140 150
EKYVGKDITL KALIEASMTY SDNTANNKII KEIGGIKKVK QRLKELGDKV
160 170 180 190 200
TNPVRYEIEL NYYSPKSKKD TSTPAAFGKT LNKLIANGKL SKENKKFLLD
210 220 230 240 250
LMLNNKSGDT LIKDGVPKDY KVADKSGQAI TYASRNDVAF VYPKGQSEPI
260 270 280
VLVIFTNKDN KSDKPNDKLI SETAKSVMKE F
Length:281
Mass (Da):31,349
Last modified:July 21, 1986 - v1
Checksum:iF82A836773C275FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04121 Genomic DNA. Translation: CAA27733.1.
X16471 Genomic DNA. Translation: CAA34491.1.
M15526 Genomic DNA. Translation: AAA98239.1.
X52734 Genomic DNA. Translation: CAA36953.1.
PIRiA01002. PNSAP.
RefSeqiNP_878023.1. NC_005054.1.
WP_000733283.1. NZ_LXFD01000005.1.
YP_003329488.1. NC_013550.1.
YP_006937602.1. NC_013319.1.
YP_006937751.1. NC_013323.1.
YP_006938263.1. NC_013337.1.
YP_006938770.1. NC_013352.1.
YP_008709799.1. NC_022598.1.

Genome annotation databases

GeneIDi13874750.
13874903.
13875430.
13875951.
17363235.
24246438.
2598287.
8655740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04121 Genomic DNA. Translation: CAA27733.1.
X16471 Genomic DNA. Translation: CAA34491.1.
M15526 Genomic DNA. Translation: AAA98239.1.
X52734 Genomic DNA. Translation: CAA36953.1.
PIRiA01002. PNSAP.
RefSeqiNP_878023.1. NC_005054.1.
WP_000733283.1. NZ_LXFD01000005.1.
YP_003329488.1. NC_013550.1.
YP_006937602.1. NC_013319.1.
YP_006937751.1. NC_013323.1.
YP_006938263.1. NC_013337.1.
YP_006938770.1. NC_013352.1.
YP_008709799.1. NC_022598.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ALQX-ray1.80A24-244[»]
1BLCX-ray2.20A25-281[»]
1BLHX-ray2.30A25-281[»]
1BLPX-ray2.30A25-281[»]
1DJAX-ray1.90A25-281[»]
1DJBX-ray2.10A25-281[»]
1DJCX-ray2.00A25-281[»]
1GHIX-ray2.30A25-281[»]
1GHMX-ray1.86A25-281[»]
1GHPX-ray1.76A25-281[»]
1KGEX-ray2.00A25-281[»]
1KGFX-ray2.20A25-281[»]
1KGGX-ray2.30A24-281[»]
1OMEX-ray2.30A/B25-281[»]
1PIOX-ray2.80A/B25-281[»]
3BLMX-ray2.00A25-281[»]
ProteinModelPortaliP00807.
SMRiP00807.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP00807.
ChEMBLiCHEMBL4114.
DrugBankiDB00766. Clavulanate.
DB01598. Imipenem.

Proteomic databases

PRIDEiP00807.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13874750.
13874903.
13875430.
13875951.
17363235.
24246438.
2598287.
8655740.

Enzyme and pathway databases

SABIO-RKP00807.

Miscellaneous databases

EvolutionaryTraceiP00807.
PROiP00807.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLAC_STAAU
AccessioniPrimary (citable) accession number: P00807
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.