Beta-lactamase precursor - Staphylococcus aureus

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Reviewed, UniProtKB/Swiss-Prot P00807 (BLAC_STAAU)

Last modified September 22, 2009. Version 89. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Beta-lactamase
    EC=3.5.2.6
Alternative name(s):
    Penicillinase

Gene names

Name:

blaZ

Encoded on

Plasmid pI258 Ref.1 Ref.4

Organism

Staphylococcus aureus

Taxonomic identifier

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A beta-lactam + H₂O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	3D-structure Direct protein sequencing Plasmid Transposable element
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

24

Chain

257

Beta-lactamase

PRO_0000017020

Regions

Region

3

Substrate binding

Sites

Active site

1

Acyl-ester intermediate

Secondary structure

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281

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

P00807-1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: F82A836773C275FE
Show »

281

31,349

        10         20         30         40         50         60 
MKKLIFLIVI ALVLSACNSN SSHAKELNDL EKKYNAHIGV YALDTKSGKE VKFNSDKRFA 

        70         80         90        100        110        120 
YASTSKAINS AILLEQVPYN KLNKKVHINK DDIVAYSPIL EKYVGKDITL KALIEASMTY 

       130        140        150        160        170        180 
SDNTANNKII KEIGGIKKVK QRLKELGDKV TNPVRYEIEL NYYSPKSKKD TSTPAAFGKT 

       190        200        210        220        230        240 
LNKLIANGKL SKENKKFLLD LMLNNKSGDT LIKDGVPKDY KVADKSGQAI TYASRNDVAF 

       250        260        270        280 
VYPKGQSEPI VLVIFTNKDN KSDKPNDKLI SETAKSVMKE F

References

[1]	"Nucleotide sequence of the Staphylococcus aureus PC1 beta-lactamase gene." Chan P.T. Nucleic Acids Res. 14:5940-5940(1986) [PubMed: 3488540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PC-1.
[2]	"Nucleotide sequence of the blaZ gene of the Staphylococcus aureus beta-lactamase transposon Tn4002." Gillspie M.T., Skurray R.A. Nucleic Acids Res. 17:8854-8854(1989) [PubMed: 2555777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SK456. Transposon: Tn4002.
[3]	"Tn552, a novel transposable element from Staphylococcus aureus." Rowland S.J., Dyke K.G.H. Mol. Microbiol. 4:961-975(1990) [PubMed: 2170815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCTC 9789. Transposon: Tn552.
[4]	"Nucleotide sequence and expression of the beta-lactamase gene from Staphylococcus aureus plasmid pI258 in Escherichia coli, Bacillus subtilis, and Staphylococcus aureus." Wang P.-Z., Novick R.P. J. Bacteriol. 169:1763-1766(1987) [PubMed: 3104315] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Unique features in the ribosome binding site sequence of the Gram-positive Staphylococcus aureus beta-lactamase gene." McLaughlin J.R., Murray C.L., Rabinowitz J.C. J. Biol. Chem. 256:11283-11291(1981) [PubMed: 6793593] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-47.
[6]	"The amino acid sequence of Staphylococcus aureus penicillinase." Ambler R.P. Biochem. J. 151:197-218(1975) [PubMed: 1218078] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-281.
[7]	"Bacterial resistance to beta-lactam antibiotics: crystal structure of beta-lactamase from Staphylococcus aureus PC1 at 2.5-A resolution." Herzberg O., Moult J. Science 236:694-701(1987) [PubMed: 3107125] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[8]	"Refined crystal structure of beta-lactamase from Staphylococcus aureus PC1 at 2.0-A resolution." Herzberg O. J. Mol. Biol. 217:701-719(1991) [PubMed: 2005620] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[9]	"Role of the omega-loop in the activity, substrate specificity, and structure of class A beta-lactamase." Banerjee S., Pieper U., Kapadia G., Pannell L.K., Herzberg O. Biochemistry 37:3286-3296(1998) [PubMed: 9521648] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[10]	"Relocation of the catalytic carboxylate group in class A beta-lactamase: the structure and function of the mutant enzyme Glu166-->Gln:Asn170-->Asp." Chen C.C., Herzberg O. Protein Eng. 12:573-579(1999) [PubMed: 10436083] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

X04121 Genomic DNA. Translation: CAA27733.1.
X16471 Genomic DNA. Translation: CAA34491.1.
M15526 Genomic DNA. Translation: AAA98239.1.
X52734 Genomic DNA. Translation: CAA36953.1.

PIR

PNSAP. A01002.

RefSeq

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ALQ	X-ray	1.80	A	24-281	[»]
1BLC	X-ray	2.20	A	25-281	[»]
1BLH	X-ray	2.30	A	25-281	[»]
1BLP	X-ray	2.30	A	25-281	[»]
1DJA	X-ray	1.90	A	25-281	[»]
1DJB	X-ray	2.10	A	25-281	[»]
1DJC	X-ray	2.00	A	25-281	[»]
1GHI	X-ray	2.30	A	25-281	[»]
1GHM	X-ray	1.86	A	25-281	[»]
1GHP	X-ray	1.76	A	25-281	[»]
1KGE	X-ray	2.00	A	25-281	[»]
1KGF	X-ray	2.20	A	25-281	[»]
1KGG	X-ray	2.30	A	25-281	[»]
1OME	X-ray	2.30	A/B	25-281	[»]
1PIO	X-ray	2.80	A/B	25-281	[»]
3BLM	X-ray	2.00	A	25-281	[»]

ModBase

Genome annotation databases

GeneID

Enzyme and pathway databases

BRENDA

Family and domain databases

InterPro

IPR001466. Beta_lactamase-related.
IPR000871. Beta_lactamase_A/D.
[Graphical view]

Pfam

PF00144. Beta-lactamase. 1 hit.
[Graphical view]

PRINTS

PR00118. BLACTAMASEA.

PROSITE

PS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit. Uncertain.
[Graphical view]

ProtoNet

Other Resources

DrugBank

DB00766. Clavulanate.
DB01598. Imipenem.

Entry information

Entry name

BLAC_STAAU

Accession

Primary (citable) accession number: P00807

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	September 22, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents