Reviewed, UniProtKB/Swiss-Prot P00804 (LSPA_ECOLI)

Names and origin

Protein names

Recommended name:
    Lipoprotein signal peptidase
    EC=3.4.23.36
Alternative name(s):
    Prolipoprotein signal peptidase
    Signal peptidase II
      Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	b0027, JW0025

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein. HAMAP MF_00161
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 164	164	Lipoprotein signal peptidase HAMAP MF_00161		PRO_0000178778
Regions
	Topological domain	1 – 11	11	Cytoplasmic Probable
	Transmembrane	12 – 26	15	Probable
	Topological domain	27 – 69	43	Periplasmic Probable
	Transmembrane	70 – 88	19	Probable
	Topological domain	89 – 95	7	Cytoplasmic Probable
	Transmembrane	96 – 113	18	Probable
	Topological domain	114 – 141	28	Periplasmic Probable
	Transmembrane	142 – 159	18	Probable
	Topological domain	160 – 164	5	Cytoplasmic Probable
Sites
	Active site	114	1	By similarity
	Active site	141	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00804-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 65773737DDAA3EB1	FASTA	164	18,156
	10 20 30 40 50 60 MSQSICSTGL RWLWLVVVVL IIDLGSKYLI LQNFALGDTV PLFPSLNLHY ARNYGAAFSF 70 80 90 100 110 120 LADSGGWQRW FFAGIAIGIS VILAVMMYRS KATQKLNNIA YALIIGGALG NLFDRLWHGF 130 140 150 160 VVDMIDFYVG DWHFATFNLA DTAICVGAAL IVLEGFLPSR AKKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the lspA gene, the structural gene for lipoprotein signal peptidase of Escherichia coli." Yu F., Yamada H., Daishima K., Mizushima S. FEBS Lett. 173:264-268(1984) [PubMed: 6378662] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of the Escherichia coli prolipoprotein signal peptidase (lsp) gene." Innis M.A., Tokunaga M., Williams M.E., Loranger J.M., Chang S.-Y., Chang S., Wu H.C. Proc. Natl. Acad. Sci. U.S.A. 81:3708-3712(1984) [PubMed: 6374664] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Nucleotide sequence of the lsp-dapB interval in Escherichia coli." Bouvier J., Stragier P. Nucleic Acids Res. 19:180-180(1991) [PubMed: 2011499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 159-164. Strain: K12.
[7]	"Membrane topology of Escherichia coli prolipoprotein signal peptidase (signal peptidase II)." Munoa F.J., Miller K.W., Beers R., Graham M., Wu H.C. J. Biol. Chem. 266:17667-17672(1991) [PubMed: 1894646] [Abstract] Cited for: TOPOLOGY.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X00776 Genomic DNA. Translation: CAA25353.1. K01990 Genomic DNA. Translation: AAA24092.1. U00096 Genomic DNA. Translation: AAC73138.1. AP009048 Genomic DNA. Translation: BAB96596.1. X54945 Genomic DNA. Translation: CAA38705.1.
PIR	ZPECL. C64723.
RefSeq	AP_000691.1. NP_414568.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P00804.
Genome annotation databases
GeneID	944800.
GenomeReviews	Gene locus JW0025 in contig AP009048_GR. Gene locus b0027 in contig U00096_GR.
KEGG	ecj:JW0025. eco:b0027.
Organism-specific databases
EchoBASE	EB0543.
EcoGene	EG10548. lspA.
CMR	Search...
Phylogenomic databases
HOGENOM	P00804.
OMA	ADSSGWQ.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10548-MON.
Gene expression databases
Genevestigator	P00804.
Family and domain databases
HAMAP	MF_00161. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
ProDom	PD004304. Peptidase_A8. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_ECOLI

Accession

Primary (citable) accession number: P00804

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 13, 1987
Last modified:	November 3, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)