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Protein

Lipoprotein signal peptidase

Gene

lspA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein specifically catalyzes the removal of signal peptides from prolipoproteins.3 Publications

Catalytic activityi

Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.1 Publication

Enzyme regulationi

Enzyme activity is inhibited by globomycin, a cyclic peptide antibiotic.1 Publication

pH dependencei

Optimum pH is 7.9.1 Publication

Temperature dependencei

Optimum temperature is 37-45 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei114By similarity1
Active sitei141By similarity1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: UniProtKB-HAMAP
  • peptidase activity Source: EcoCyc

GO - Biological processi

  • signal peptide processing Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciEcoCyc:EG10548-MONOMER.
ECOL316407:JW0025-MONOMER.
MetaCyc:EG10548-MONOMER.

Protein family/group databases

MEROPSiA08.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein signal peptidase (EC:3.4.23.36)
Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name:
SPase II
Gene namesi
Name:lspA
Synonyms:lsp
Ordered Locus Names:b0027, JW0025
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10548. lspA.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicCuratedAdd BLAST11
Transmembranei12 – 26HelicalCuratedAdd BLAST15
Topological domaini27 – 69PeriplasmicCuratedAdd BLAST43
Transmembranei70 – 88HelicalCuratedAdd BLAST19
Topological domaini89 – 95CytoplasmicCurated7
Transmembranei96 – 113HelicalCuratedAdd BLAST18
Topological domaini114 – 141PeriplasmicCuratedAdd BLAST28
Transmembranei142 – 159HelicalCuratedAdd BLAST18
Topological domaini160 – 164CytoplasmicCurated5

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001787781 – 164Lipoprotein signal peptidaseAdd BLAST164

Proteomic databases

PaxDbiP00804.
PRIDEiP00804.

Interactioni

Protein-protein interaction databases

BioGridi4259402. 212 interactors.
DIPiDIP-10129N.
STRINGi511145.b0027.

Structurei

3D structure databases

ProteinModelPortaliP00804.
SMRiP00804.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A8 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105M02. Bacteria.
COG0597. LUCA.
HOGENOMiHOG000096993.
InParanoidiP00804.
KOiK03101.
OMAiDFASKQW.
PhylomeDBiP00804.

Family and domain databases

HAMAPiMF_00161. LspA. 1 hit.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PANTHERiPTHR33695:SF1. PTHR33695:SF1. 1 hit.
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSICSTGL RWLWLVVVVL IIDLGSKYLI LQNFALGDTV PLFPSLNLHY
60 70 80 90 100
ARNYGAAFSF LADSGGWQRW FFAGIAIGIS VILAVMMYRS KATQKLNNIA
110 120 130 140 150
YALIIGGALG NLFDRLWHGF VVDMIDFYVG DWHFATFNLA DTAICVGAAL
160
IVLEGFLPSR AKKQ
Length:164
Mass (Da):18,156
Last modified:August 13, 1987 - v1
Checksum:i65773737DDAA3EB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00776 Genomic DNA. Translation: CAA25353.1.
K01990 Genomic DNA. Translation: AAA24092.1.
U00096 Genomic DNA. Translation: AAC73138.1.
AP009048 Genomic DNA. Translation: BAB96596.1.
X54945 Genomic DNA. Translation: CAA38705.1.
PIRiC64723. ZPECL.
RefSeqiNP_414568.1. NC_000913.3.
WP_000083372.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73138; AAC73138; b0027.
BAB96596; BAB96596; BAB96596.
GeneIDi944800.
KEGGiecj:JW0025.
eco:b0027.
PATRICi32115145. VBIEscCol129921_0024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00776 Genomic DNA. Translation: CAA25353.1.
K01990 Genomic DNA. Translation: AAA24092.1.
U00096 Genomic DNA. Translation: AAC73138.1.
AP009048 Genomic DNA. Translation: BAB96596.1.
X54945 Genomic DNA. Translation: CAA38705.1.
PIRiC64723. ZPECL.
RefSeqiNP_414568.1. NC_000913.3.
WP_000083372.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP00804.
SMRiP00804.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259402. 212 interactors.
DIPiDIP-10129N.
STRINGi511145.b0027.

Protein family/group databases

MEROPSiA08.001.

Proteomic databases

PaxDbiP00804.
PRIDEiP00804.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73138; AAC73138; b0027.
BAB96596; BAB96596; BAB96596.
GeneIDi944800.
KEGGiecj:JW0025.
eco:b0027.
PATRICi32115145. VBIEscCol129921_0024.

Organism-specific databases

EchoBASEiEB0543.
EcoGeneiEG10548. lspA.

Phylogenomic databases

eggNOGiENOG4105M02. Bacteria.
COG0597. LUCA.
HOGENOMiHOG000096993.
InParanoidiP00804.
KOiK03101.
OMAiDFASKQW.
PhylomeDBiP00804.

Enzyme and pathway databases

BioCyciEcoCyc:EG10548-MONOMER.
ECOL316407:JW0025-MONOMER.
MetaCyc:EG10548-MONOMER.

Miscellaneous databases

PROiP00804.

Family and domain databases

HAMAPiMF_00161. LspA. 1 hit.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PANTHERiPTHR33695:SF1. PTHR33695:SF1. 1 hit.
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLSPA_ECOLI
AccessioniPrimary (citable) accession number: P00804
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: November 2, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.