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Protein

Signal peptidase I

Gene

lepB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei911
Active sitei1461

GO - Molecular functioni

  • endopeptidase activity Source: EcoCyc
  • peptidase activity Source: EcoliWiki
  • serine-type endopeptidase activity Source: EcoCyc
  • toxic substance binding Source: EcoliWiki

GO - Biological processi

  • protein processing Source: EcoliWiki
  • proteolysis Source: EcoliWiki
  • signal peptide processing Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Enzyme and pathway databases

BioCyciEcoCyc:EG10530-MONOMER.
ECOL316407:JW2552-MONOMER.
MetaCyc:EG10530-MONOMER.
BRENDAi3.4.21.89. 2026.
SABIO-RKP00803.

Protein family/group databases

MEROPSiS26.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal peptidase I (EC:3.4.21.89)
Short name:
SPase I
Alternative name(s):
Leader peptidase I
Gene namesi
Name:lepB
Ordered Locus Names:b2568, JW2552
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10530. lepB.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3PeriplasmicCurated3
Transmembranei4 – 22HelicalCuratedAdd BLAST19
Topological domaini23 – 58CytoplasmicCuratedAdd BLAST36
Transmembranei59 – 77HelicalCuratedAdd BLAST19
Topological domaini78 – 324PeriplasmicCuratedAdd BLAST247

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi62E → V: Indifferent. 1
Mutagenesisi78R → E, N or L: Indifferent. 1
Mutagenesisi91S → A: Loss of activity. 1
Mutagenesisi125H → N: Indifferent. 1
Mutagenesisi128R → Q: Small effect. 1
Mutagenesisi130D → A: Indifferent. 1
Mutagenesisi144Y → F: Indifferent. 1
Mutagenesisi146K → M, D, G or S: Loss of activity. 1
Mutagenesisi147R → Q: Small effect. 1
Mutagenesisi154D → A: Loss of activity. 1
Mutagenesisi154D → N: Indifferent. 1
Mutagenesisi159D → N: Small effect. 1
Mutagenesisi171C → A: Indifferent. 1
Mutagenesisi177C → A: Indifferent. 1
Mutagenesisi236H → N: Indifferent. 1
Mutagenesisi269Y → F: Indifferent. 1
Mutagenesisi274D → A: Indifferent. 1
Mutagenesisi276R → Q: Small effect. 1
Mutagenesisi281D → A: Indifferent. 1
Mutagenesisi283R → Q: Small effect. 1

Chemistry databases

ChEMBLiCHEMBL4470.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001095061 – 324Signal peptidase IAdd BLAST324

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1Blocked amino end (Met)1 Publication1
Disulfide bondi171 ↔ 177

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP00803.
PRIDEiP00803.

Interactioni

Protein-protein interaction databases

BioGridi4259467. 97 interactors.
STRINGi511145.b2568.

Chemistry databases

BindingDBiP00803.

Structurei

Secondary structure

1324
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi82 – 86Combined sources5
Turni91 – 95Combined sources5
Beta strandi100 – 112Combined sources13
Helixi114 – 116Combined sources3
Beta strandi119 – 123Combined sources5
Beta strandi131 – 135Combined sources5
Beta strandi142 – 150Combined sources9
Beta strandi155 – 159Combined sources5
Turni160 – 163Combined sources4
Beta strandi164 – 168Combined sources5
Beta strandi174 – 176Combined sources3
Beta strandi183 – 185Combined sources3
Beta strandi189 – 198Combined sources10
Helixi200 – 202Combined sources3
Beta strandi205 – 211Combined sources7
Beta strandi214 – 216Combined sources3
Beta strandi221 – 231Combined sources11
Beta strandi234 – 240Combined sources7
Helixi248 – 250Combined sources3
Beta strandi261 – 263Combined sources3
Beta strandi268 – 272Combined sources5
Helixi282 – 285Combined sources4
Helixi290 – 292Combined sources3
Beta strandi293 – 304Combined sources12
Beta strandi310 – 312Combined sources3
Beta strandi313 – 315Combined sources3
Helixi317 – 319Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B12X-ray1.95A/B/C/D77-324[»]
1KN9X-ray2.40A/B/C/D77-324[»]
1T7DX-ray2.47A/B76-324[»]
3IIQX-ray2.00A/B77-324[»]
3S04X-ray2.44A/B76-324[»]
ProteinModelPortaliP00803.
SMRiP00803.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00803.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S26 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C3F. Bacteria.
COG0681. LUCA.
HOGENOMiHOG000003674.
InParanoidiP00803.
KOiK03100.
OMAiFKWAPAR.
PhylomeDBiP00803.

Family and domain databases

Gene3Di2.10.109.10. 2 hits.
2.170.230.10. 1 hit.
InterProiIPR000223. Pept_S26A_signal_pept_1.
IPR019758. Pept_S26A_signal_pept_1_CS.
IPR019757. Pept_S26A_signal_pept_1_Lys-AS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR019533. Peptidase_S26.
IPR019766. Peptidase_S26A_all-beta_subdom.
[Graphical view]
PANTHERiPTHR12383. PTHR12383. 2 hits.
PfamiPF00717. Peptidase_S24. 1 hit.
PF10502. Peptidase_S26. 1 hit.
[Graphical view]
PRINTSiPR00727. LEADERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02227. sigpep_I_bact. 1 hit.
PROSITEiPS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. 1 hit.
PS00761. SPASE_I_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANMFALILV IATLVTGILW CVDKFFFAPK RRERQAAAQA AAGDSLDKAT
60 70 80 90 100
LKKVAPKPGW LETGASVFPV LAIVLIVRSF IYEPFQIPSG SMMPTLLIGD
110 120 130 140 150
FILVEKFAYG IKDPIYQKTL IETGHPKRGD IVVFKYPEDP KLDYIKRAVG
160 170 180 190 200
LPGDKVTYDP VSKELTIQPG CSSGQACENA LPVTYSNVEP SDFVQTFSRR
210 220 230 240 250
NGGEATSGFF EVPKNETKEN GIRLSERKET LGDVTHRILT VPIAQDQVGM
260 270 280 290 300
YYQQPGQQLA TWIVPPGQYF MMGDNRDNSA DSRYWGFVPE ANLVGRATAI
310 320
WMSFDKQEGE WPTGLRLSRI GGIH
Length:324
Mass (Da):35,960
Last modified:November 1, 1997 - v2
Checksum:i9419710D7212E660
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42 – 43AG → R (PubMed:6311837).Curated2
Sequence conflicti42 – 43AG → R (Ref. 2) Curated2
Sequence conflicti123T → N (PubMed:6311837).Curated1
Sequence conflicti123T → N (Ref. 2) Curated1
Sequence conflicti183V → A (PubMed:6311837).Curated1
Sequence conflicti183V → A (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00426 Genomic DNA. Translation: AAA24064.1.
D64044 Genomic DNA. Translation: BAA10915.1.
U00096 Genomic DNA. Translation: AAC75621.1.
AP009048 Genomic DNA. Translation: BAE76744.1.
PIRiG65034. ZPECS.
RefSeqiNP_417063.1. NC_000913.3.
WP_000002541.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75621; AAC75621; b2568.
BAE76744; BAE76744; BAE76744.
GeneIDi947040.
KEGGiecj:JW2552.
eco:b2568.
PATRICi32120535. VBIEscCol129921_2670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00426 Genomic DNA. Translation: AAA24064.1.
D64044 Genomic DNA. Translation: BAA10915.1.
U00096 Genomic DNA. Translation: AAC75621.1.
AP009048 Genomic DNA. Translation: BAE76744.1.
PIRiG65034. ZPECS.
RefSeqiNP_417063.1. NC_000913.3.
WP_000002541.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B12X-ray1.95A/B/C/D77-324[»]
1KN9X-ray2.40A/B/C/D77-324[»]
1T7DX-ray2.47A/B76-324[»]
3IIQX-ray2.00A/B77-324[»]
3S04X-ray2.44A/B76-324[»]
ProteinModelPortaliP00803.
SMRiP00803.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259467. 97 interactors.
STRINGi511145.b2568.

Chemistry databases

BindingDBiP00803.
ChEMBLiCHEMBL4470.

Protein family/group databases

MEROPSiS26.001.

Proteomic databases

PaxDbiP00803.
PRIDEiP00803.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75621; AAC75621; b2568.
BAE76744; BAE76744; BAE76744.
GeneIDi947040.
KEGGiecj:JW2552.
eco:b2568.
PATRICi32120535. VBIEscCol129921_2670.

Organism-specific databases

EchoBASEiEB0525.
EcoGeneiEG10530. lepB.

Phylogenomic databases

eggNOGiENOG4105C3F. Bacteria.
COG0681. LUCA.
HOGENOMiHOG000003674.
InParanoidiP00803.
KOiK03100.
OMAiFKWAPAR.
PhylomeDBiP00803.

Enzyme and pathway databases

BioCyciEcoCyc:EG10530-MONOMER.
ECOL316407:JW2552-MONOMER.
MetaCyc:EG10530-MONOMER.
BRENDAi3.4.21.89. 2026.
SABIO-RKP00803.

Miscellaneous databases

EvolutionaryTraceiP00803.
PROiP00803.

Family and domain databases

Gene3Di2.10.109.10. 2 hits.
2.170.230.10. 1 hit.
InterProiIPR000223. Pept_S26A_signal_pept_1.
IPR019758. Pept_S26A_signal_pept_1_CS.
IPR019757. Pept_S26A_signal_pept_1_Lys-AS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR019533. Peptidase_S26.
IPR019766. Peptidase_S26A_all-beta_subdom.
[Graphical view]
PANTHERiPTHR12383. PTHR12383. 2 hits.
PfamiPF00717. Peptidase_S24. 1 hit.
PF10502. Peptidase_S26. 1 hit.
[Graphical view]
PRINTSiPR00727. LEADERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02227. sigpep_I_bact. 1 hit.
PROSITEiPS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. 1 hit.
PS00761. SPASE_I_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEP_ECOLI
AccessioniPrimary (citable) accession number: P00803
Secondary accession number(s): P78098, Q2MAG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.