P00801 (PRLB_LYSEN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-lytic metalloendopeptidase EC=3.4.24.32 Alternative name(s): Beta-lytic protease
Organism	Lysobacter enzymogenes
Taxonomic identifier	69 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Lysobacter

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Cleavage of N-acetylmuramoyl-|-Ala, and of the insulin B chain at 23-Gly-|-Phe-24 > 18-Val-|-Cys(SO3H).
Cofactor	Binds 1 zinc ion per subunit.
Sequence similarities	Belongs to the peptidase M23A family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Beta-lytic metalloendopeptidase		PRO_0000165903
Sites
	Metal binding	120	1	Zinc Potential
	Metal binding	122	1	Zinc Potential
Amino acid modifications
	Disulfide bond	65 ↔ 111		Ref.1
	Disulfide bond	155 ↔ 168		Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P00801 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 9EFCD3DD45FD2870	FASTA	178	19,100
	10 20 30 40 50 60 SPNGLLQFPF PRGASWHVGG AHTNTGSGNY PMSSLDMSRG GGSNQNGNWV SASAAGGSFK 70 80 90 100 110 120 RHSSCFAEIV HTGGWSTTYY HLMNIQYNTG ANVSMNTAIA NAPNTQAQAL CNGGQSTGPH 130 140 150 160 170 QHWSLKQNGS FYHLNGTYLS GYRITATGSS YDTNCSRFYL TKNGQNYCYG YYVNPGPN

References

[1]	Damaglou A.P., Allen L.C., Whitaker D.R. Submitted (MAR-1974) to the PIR data bank Cited for: PROTEIN SEQUENCE, DISULFIDE BONDS. Strain: ATCC 29487 / DSM 2043 / LMG 8762 / UASM 495 / VKM B-2235.

Cross-references

Sequence databases
PIR	LYYXB4. A00994.
3D structure databases
ProteinModelPortal	P00801.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011055. Dup_hybrid_motif. IPR000841. Pept_M23A_Blytic. IPR016047. Peptidase_M23. [Graphical view]
Pfam	PF01551. Peptidase_M23. 1 hit. [Graphical view]
PRINTS	PR00933. BLYTICPTASE.
SUPFAM	SSF51261. Dup_hybrid_motif. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PRLB_LYSEN

Accession

Primary (citable) accession number: P00801

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 16, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program