Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mucorpepsin

Gene
N/A
Organism
Rhizomucor miehei
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme, capable of clotting milk is frequently used for cheese production.

Catalytic activityi

Hydrolysis of proteins, favoring hydrophobic residues at P1 and P1'. Clots milk. Does not accept Lys at P1, and hence does not activate trypsinogen.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei107PROSITE-ProRule annotation1
Active sitei306PROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Protein family/group databases

MEROPSiA01.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Mucorpepsin (EC:3.4.23.23)
Alternative name(s):
Mucor rennin
OrganismiRhizomucor miehei
Taxonomic identifieri4839 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMucoromycotinaMucoralesLichtheimiaceaeRhizomucor

Pathology & Biotechi

Protein family/group databases

Allergomei3880. Rhi m AP.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
PropeptideiPRO_000002589323 – 69Activation peptide1 PublicationAdd BLAST47
ChainiPRO_000002589470 – 430Mucorpepsin1 PublicationAdd BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi120 ↔ 1261 Publication
Glycosylationi148N-linked (GlcNAc...)1 Publication1
Glycosylationi257N-linked (GlcNAc...)1 Publication1
Disulfide bondi341 ↔ 3851 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Structurei

Secondary structure

1430
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi76 – 82Combined sources7
Turni84 – 86Combined sources3
Beta strandi89 – 95Combined sources7
Turni96 – 99Combined sources4
Beta strandi100 – 107Combined sources8
Beta strandi114 – 117Combined sources4
Helixi122 – 124Combined sources3
Helixi134 – 136Combined sources3
Beta strandi141 – 149Combined sources9
Beta strandi156 – 169Combined sources14
Beta strandi171 – 183Combined sources13
Helixi186 – 188Combined sources3
Beta strandi194 – 196Combined sources3
Beta strandi200 – 203Combined sources4
Helixi207 – 209Combined sources3
Helixi211 – 216Combined sources6
Helixi223 – 229Combined sources7
Beta strandi232 – 241Combined sources10
Beta strandi244 – 254Combined sources11
Helixi258 – 260Combined sources3
Beta strandi261 – 263Combined sources3
Beta strandi266 – 269Combined sources4
Beta strandi281 – 289Combined sources9
Beta strandi292 – 306Combined sources15
Beta strandi311 – 313Combined sources3
Turni316 – 319Combined sources4
Helixi322 – 326Combined sources5
Beta strandi331 – 333Combined sources3
Beta strandi334 – 336Combined sources3
Beta strandi338 – 340Combined sources3
Turni341 – 345Combined sources5
Beta strandi350 – 357Combined sources8
Beta strandi364 – 371Combined sources8
Helixi372 – 374Combined sources3
Beta strandi375 – 392Combined sources18
Beta strandi394 – 396Combined sources3
Beta strandi398 – 400Combined sources3
Helixi401 – 404Combined sources4
Beta strandi407 – 412Combined sources6
Turni413 – 416Combined sources4
Beta strandi417 – 423Combined sources7
Helixi425 – 427Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ASIX-ray2.15A70-430[»]
2RMPX-ray2.70A70-430[»]
ProteinModelPortaliP00799.
SMRiP00799.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00799.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini89 – 421Peptidase A1PROSITE-ProRule annotationAdd BLAST333

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFSQITSAI LLTAASLSLT TARPVSKQSE SKDKLLALPL TSVSRKFSQT
60 70 80 90 100
KFGQQQLAEK LAGLKPFSEA AADGSVDTPG YYDFDLEEYA IPVSIGTPGQ
110 120 130 140 150
DFLLLFDTGS SDTWVPHKGC TKSEGCVGSR FFDPSASSTF KATNYNLNIT
160 170 180 190 200
YGTGGANGLY FEDSIAIGDI TVTKQILAYV DNVRGPTAEQ SPNADIFLDG
210 220 230 240 250
LFGAAYPDNT AMEAEYGSTY NTVHVNLYKQ GLISSPLFSV YMNTNSGTGE
260 270 280 290 300
VVFGGVNNTL LGGDIAYTDV MSRYGGYYFW DAPVTGITVD GSAAVRFSRP
310 320 330 340 350
QAFTIDTGTN FFIMPSSAAS KIVKAALPDA TETQQGWVVP CASYQNSKST
360 370 380 390 400
ISIVMQKSGS SSDTIEISVP VSKMLLPVDQ SNETCMFIIL PDGGNQYIVG
410 420 430
NLFLRFFVNV YDFGNNRIGF APLASAYENE
Length:430
Mass (Da):46,168
Last modified:January 1, 1988 - v1
Checksum:i1431DB2435B71CEF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136A → T AA sequence (Ref. 3) Curated1
Sequence conflicti157N → D AA sequence (Ref. 3) Curated1
Sequence conflicti163 – 169Missing AA sequence (Ref. 3) Curated7
Sequence conflicti252Missing AA sequence (Ref. 3) Curated1
Sequence conflicti376Missing AA sequence (Ref. 3) Curated1
Sequence conflicti392D → N AA sequence (Ref. 3) Curated1
Sequence conflicti399V → Y AA sequence (Ref. 3) Curated1
Sequence conflicti411Missing AA sequence (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti262G → S.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18411 mRNA. Translation: AAA33423.1.
M15267 Genomic DNA. Translation: AAA33421.1.
PIRiA29039. CMUMF.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18411 mRNA. Translation: AAA33423.1.
M15267 Genomic DNA. Translation: AAA33421.1.
PIRiA29039. CMUMF.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ASIX-ray2.15A70-430[»]
2RMPX-ray2.70A70-430[»]
ProteinModelPortaliP00799.
SMRiP00799.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3880. Rhi m AP.
MEROPSiA01.013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP00799.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCARP_RHIMI
AccessioniPrimary (citable) accession number: P00799
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.