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P00796

- RENI2_MOUSE

UniProt

P00796 - RENI2_MOUSE

Protein

Renin-2

Gene

Ren2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Renin is a highly specific endopeptidase, related to pepsin, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Its function in the salivary gland is not understood.

    Catalytic activityi

    Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei101 – 1011
    Active sitei286 – 2861

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: UniProtKB-KW
    2. endopeptidase activity Source: MGI

    GO - Biological processi

    1. positive regulation of blood pressure Source: MGI

    Keywords - Molecular functioni

    Aspartyl protease, Hydrolase, Protease

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12952.

    Protein family/group databases

    MEROPSiA01.008.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Renin-2 (EC:3.4.23.15)
    Alternative name(s):
    Angiotensinogenase
    Submandibular gland renin
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Ren2
    Synonyms:Ren-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:97899. Ren2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: MGI

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525CuratedAdd
    BLAST
    Propeptidei26 – 6338Activation peptide1 PublicationPRO_0000026095Add
    BLAST
    Chaini64 – 401338Renin-2PRO_0000026096Add
    BLAST
    Chaini64 – 351288Renin-2 heavy chainPRO_0000026097Add
    BLAST
    Chaini354 – 40148Renin-2 light chainPRO_0000026098Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi114 ↔ 121
    Disulfide bondi277 ↔ 281
    Disulfide bondi320 ↔ 357

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Zymogen

    Proteomic databases

    PRIDEiP00796.

    Miscellaneous databases

    PMAP-CutDBP00796.

    Expressioni

    Tissue specificityi

    Submandibular gland.

    Gene expression databases

    GenevestigatoriP00796.

    Interactioni

    Subunit structurei

    Dimer of a heavy chain and a light chain joined by a disulfide bond.

    Structurei

    Secondary structure

    1
    401
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi71 – 788
    Turni79 – 813
    Beta strandi82 – 898
    Turni90 – 934
    Beta strandi94 – 1018
    Beta strandi107 – 1115
    Helixi119 – 1235
    Helixi129 – 1313
    Beta strandi136 – 14611
    Beta strandi149 – 16214
    Beta strandi165 – 17612
    Helixi179 – 1824
    Beta strandi186 – 1927
    Helixi196 – 1983
    Helixi200 – 2023
    Helixi206 – 2127
    Beta strandi216 – 22510
    Beta strandi228 – 2314
    Beta strandi234 – 2407
    Helixi243 – 2453
    Beta strandi246 – 2549
    Turni258 – 2614
    Beta strandi262 – 2709
    Beta strandi281 – 2855
    Beta strandi289 – 2946
    Helixi296 – 30611
    Beta strandi309 – 3124
    Beta strandi315 – 3195
    Helixi320 – 3256
    Beta strandi329 – 3335
    Beta strandi336 – 3405
    Helixi342 – 3454
    Beta strandi356 – 3638
    Turni368 – 3703
    Beta strandi374 – 3763
    Helixi378 – 3814
    Beta strandi384 – 3896
    Turni390 – 3934
    Beta strandi394 – 4007

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SMRX-ray2.00A/C/E/G67-401[»]
    ProteinModelPortaliP00796.
    SMRiP00796. Positions 26-401.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00796.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase A1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG000482.
    InParanoidiP00796.
    KOiK01380.

    Family and domain databases

    Gene3Di2.40.70.10. 2 hits.
    InterProiIPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR012848. Aspartic_peptidase_N.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view]
    PANTHERiPTHR13683. PTHR13683. 1 hit.
    PfamiPF07966. A1_Propeptide. 1 hit.
    PF00026. Asp. 1 hit.
    [Graphical view]
    PRINTSiPR00792. PEPSIN.
    SUPFAMiSSF50630. SSF50630. 1 hit.
    PROSITEiPS00141. ASP_PROTEASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00796-1 [UniParc]FASTAAdd to Basket

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    MDRRRMPLWA LLLLWSPCTF SLPTGTTFER IPLKKMPSVR EILEERGVDM    50
    TRLSAEWDVF TKRSSLTDLI SPVVLTNYLN SQYYGEIGIG TPPQTFKVIF 100
    DTGSANLWVP STKCSRLYLA CGIHSLYESS DSSSYMENGD DFTIHYGSGR 150
    VKGFLSQDSV TVGGITVTQT FGEVTELPLI PFMLAQFDGV LGMGFPAQAV 200
    GGVTPVFDHI LSQGVLKEKV FSVYYNRGPH LLGGEVVLGG SDPEHYQGDF 250
    HYVSLSKTDS WQITMKGVSV GSSTLLCEEG CEVVVDTGSS FISAPTSSLK 300
    LIMQALGAKE KRLHEYVVSC SQVPTLPDIS FNLGGRAYTL SSTDYVLQYP 350
    NRRDKLCTVA LHAMDIPPPT GPVWVLGATF IRKFYTEFDR HNNRIGFALA 400
    R 401
    Length:401
    Mass (Da):44,283
    Last modified:July 21, 1986 - v1
    Checksum:iD938931F91F82980
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131L → W in AAA40047. (PubMed:2691339)Curated
    Sequence conflicti99 – 991I → M in AAA40050. (PubMed:6283373)Curated
    Sequence conflicti195 – 1984FPAQ → LSRS in AAA40050. (PubMed:6283373)Curated
    Sequence conflicti395 – 3951I → V in AAA40050. (PubMed:6283373)Curated

    Polymorphismi

    Present as a single-copy gene in strains such as BALB/c and C57BL/6 while some strains such as Swiss and Akr contain two copies.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00621 mRNA. Translation: AAA40050.1.
    BC011157 mRNA. Translation: AAH11157.1.
    K02597 Genomic DNA. Translation: AAA40048.1.
    M34191 Genomic DNA. Translation: AAA40046.1.
    AF237860 Genomic DNA. Translation: AAA40047.1.
    PIRiA93923. REMSS.
    I77411.
    RefSeqiNP_112470.2. NM_031193.2.
    UniGeneiMm.220955.

    Genome annotation databases

    GeneIDi19702.
    KEGGimmu:19702.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00621 mRNA. Translation: AAA40050.1 .
    BC011157 mRNA. Translation: AAH11157.1 .
    K02597 Genomic DNA. Translation: AAA40048.1 .
    M34191 Genomic DNA. Translation: AAA40046.1 .
    AF237860 Genomic DNA. Translation: AAA40047.1 .
    PIRi A93923. REMSS.
    I77411.
    RefSeqi NP_112470.2. NM_031193.2.
    UniGenei Mm.220955.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SMR X-ray 2.00 A/C/E/G 67-401 [» ]
    ProteinModelPortali P00796.
    SMRi P00796. Positions 26-401.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi A01.008.

    Proteomic databases

    PRIDEi P00796.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 19702.
    KEGGi mmu:19702.

    Organism-specific databases

    CTDi 19702.
    MGIi MGI:97899. Ren2.

    Phylogenomic databases

    HOVERGENi HBG000482.
    InParanoidi P00796.
    KOi K01380.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-12952.

    Miscellaneous databases

    EvolutionaryTracei P00796.
    NextBioi 297070.
    PMAP-CutDB P00796.
    PROi P00796.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori P00796.

    Family and domain databases

    Gene3Di 2.40.70.10. 2 hits.
    InterProi IPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR012848. Aspartic_peptidase_N.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view ]
    PANTHERi PTHR13683. PTHR13683. 1 hit.
    Pfami PF07966. A1_Propeptide. 1 hit.
    PF00026. Asp. 1 hit.
    [Graphical view ]
    PRINTSi PR00792. PEPSIN.
    SUPFAMi SSF50630. SSF50630. 1 hit.
    PROSITEi PS00141. ASP_PROTEASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: PROTEIN SEQUENCE OF 64-351 AND 354-401.
    2. "Complete amino acid sequence and maturation of the mouse submaxillary gland renin precursor."
      Panthier J.-J., Foote S., Chambraud B., Strosberg A.D., Corvol P., Rougeon F.
      Nature 298:90-92(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Salivary gland.
    4. "Mouse kidney and submaxillary gland renin genes differ in their 5' putative regulatory sequences."
      Panthier J.-J., Dreyfus M., Roux D.T.L., Rougeon F.
      Proc. Natl. Acad. Sci. U.S.A. 81:5489-5493(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
    5. "The nucleotide sequence of a mouse renin-encoding gene, Ren-1d, and its upstream region."
      Burt D.W., Mullins L.J., George H., Smith G., Brooks J., Pioli D., Brammar W.J.
      Gene 84:91-104(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
    6. "Expression of tissue-specific Ren-1 and Ren-2 genes of mice: comparative analysis of 5'-proximal flanking regions."
      Field L.J., Philbrick W.M., Howles P.N., Dickinson D.P., McGowan R.A., Gross K.W.
      Mol. Cell. Biol. 4:2321-2331(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 1-30.
    7. "Kidney and submaxillary gland renins are encoded by two non-allelic genes in Swiss mice."
      Panthier J.J., Rougeon F.
      EMBO J. 2:675-678(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 267-292.
    8. Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

    Entry informationi

    Entry nameiRENI2_MOUSE
    AccessioniPrimary (citable) accession number: P00796
    Secondary accession number(s): P70229, P97955, Q62155
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 140 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active enzyme isolated from the submandibular gland has catalytic and antigenic activities similar to renal renin.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3