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P00796

- RENI2_MOUSE

UniProt

P00796 - RENI2_MOUSE

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Protein
Renin-2
Gene
Ren2, Ren-2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Renin is a highly specific endopeptidase, related to pepsin, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Its function in the salivary gland is not understood.

Catalytic activityi

Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei101 – 1011
Active sitei286 – 2861

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB-KW
  2. endopeptidase activity Source: MGI

GO - Biological processi

  1. positive regulation of blood pressure Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12952.

Protein family/group databases

MEROPSiA01.008.

Names & Taxonomyi

Protein namesi
Recommended name:
Renin-2 (EC:3.4.23.15)
Alternative name(s):
Angiotensinogenase
Submandibular gland renin
Cleaved into the following 2 chains:
Gene namesi
Name:Ren2
Synonyms:Ren-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:97899. Ren2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Inferred
Add
BLAST
Propeptidei26 – 6338Activation peptide
PRO_0000026095Add
BLAST
Chaini64 – 401338Renin-2
PRO_0000026096Add
BLAST
Chaini64 – 351288Renin-2 heavy chain
PRO_0000026097Add
BLAST
Chaini354 – 40148Renin-2 light chain
PRO_0000026098Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi114 ↔ 121
Disulfide bondi277 ↔ 281
Disulfide bondi320 ↔ 357

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Zymogen

Proteomic databases

PRIDEiP00796.

Miscellaneous databases

PMAP-CutDBP00796.

Expressioni

Tissue specificityi

Submandibular gland.

Gene expression databases

GenevestigatoriP00796.

Interactioni

Subunit structurei

Dimer of a heavy chain and a light chain joined by a disulfide bond.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi71 – 788
Turni79 – 813
Beta strandi82 – 898
Turni90 – 934
Beta strandi94 – 1018
Beta strandi107 – 1115
Helixi119 – 1235
Helixi129 – 1313
Beta strandi136 – 14611
Beta strandi149 – 16214
Beta strandi165 – 17612
Helixi179 – 1824
Beta strandi186 – 1927
Helixi196 – 1983
Helixi200 – 2023
Helixi206 – 2127
Beta strandi216 – 22510
Beta strandi228 – 2314
Beta strandi234 – 2407
Helixi243 – 2453
Beta strandi246 – 2549
Turni258 – 2614
Beta strandi262 – 2709
Beta strandi281 – 2855
Beta strandi289 – 2946
Helixi296 – 30611
Beta strandi309 – 3124
Beta strandi315 – 3195
Helixi320 – 3256
Beta strandi329 – 3335
Beta strandi336 – 3405
Helixi342 – 3454
Beta strandi356 – 3638
Turni368 – 3703
Beta strandi374 – 3763
Helixi378 – 3814
Beta strandi384 – 3896
Turni390 – 3934
Beta strandi394 – 4007

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SMRX-ray2.00A/C/E/G67-401[»]
ProteinModelPortaliP00796.
SMRiP00796. Positions 26-401.

Miscellaneous databases

EvolutionaryTraceiP00796.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A1 family.

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG000482.
InParanoidiP00796.
KOiK01380.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00796-1 [UniParc]FASTAAdd to Basket

« Hide

MDRRRMPLWA LLLLWSPCTF SLPTGTTFER IPLKKMPSVR EILEERGVDM    50
TRLSAEWDVF TKRSSLTDLI SPVVLTNYLN SQYYGEIGIG TPPQTFKVIF 100
DTGSANLWVP STKCSRLYLA CGIHSLYESS DSSSYMENGD DFTIHYGSGR 150
VKGFLSQDSV TVGGITVTQT FGEVTELPLI PFMLAQFDGV LGMGFPAQAV 200
GGVTPVFDHI LSQGVLKEKV FSVYYNRGPH LLGGEVVLGG SDPEHYQGDF 250
HYVSLSKTDS WQITMKGVSV GSSTLLCEEG CEVVVDTGSS FISAPTSSLK 300
LIMQALGAKE KRLHEYVVSC SQVPTLPDIS FNLGGRAYTL SSTDYVLQYP 350
NRRDKLCTVA LHAMDIPPPT GPVWVLGATF IRKFYTEFDR HNNRIGFALA 400
R 401
Length:401
Mass (Da):44,283
Last modified:July 21, 1986 - v1
Checksum:iD938931F91F82980
GO

Polymorphismi

Present as a single-copy gene in strains such as BALB/c and C57BL/6 while some strains such as Swiss and Akr contain two copies.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131L → W in AAA40047. 1 Publication
Sequence conflicti99 – 991I → M in AAA40050. 1 Publication
Sequence conflicti195 – 1984FPAQ → LSRS in AAA40050. 1 Publication
Sequence conflicti395 – 3951I → V in AAA40050. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J00621 mRNA. Translation: AAA40050.1.
BC011157 mRNA. Translation: AAH11157.1.
K02597 Genomic DNA. Translation: AAA40048.1.
M34191 Genomic DNA. Translation: AAA40046.1.
AF237860 Genomic DNA. Translation: AAA40047.1.
PIRiA93923. REMSS.
I77411.
RefSeqiNP_112470.2. NM_031193.2.
UniGeneiMm.220955.

Genome annotation databases

GeneIDi19702.
KEGGimmu:19702.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J00621 mRNA. Translation: AAA40050.1 .
BC011157 mRNA. Translation: AAH11157.1 .
K02597 Genomic DNA. Translation: AAA40048.1 .
M34191 Genomic DNA. Translation: AAA40046.1 .
AF237860 Genomic DNA. Translation: AAA40047.1 .
PIRi A93923. REMSS.
I77411.
RefSeqi NP_112470.2. NM_031193.2.
UniGenei Mm.220955.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1SMR X-ray 2.00 A/C/E/G 67-401 [» ]
ProteinModelPortali P00796.
SMRi P00796. Positions 26-401.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi A01.008.

Proteomic databases

PRIDEi P00796.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 19702.
KEGGi mmu:19702.

Organism-specific databases

CTDi 19702.
MGIi MGI:97899. Ren2.

Phylogenomic databases

HOVERGENi HBG000482.
InParanoidi P00796.
KOi K01380.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-12952.

Miscellaneous databases

EvolutionaryTracei P00796.
NextBioi 297070.
PMAP-CutDB P00796.
PROi P00796.
SOURCEi Search...

Gene expression databases

Genevestigatori P00796.

Family and domain databases

Gene3Di 2.40.70.10. 2 hits.
InterProi IPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR021109. Peptidase_aspartic_dom.
[Graphical view ]
PANTHERi PTHR13683. PTHR13683. 1 hit.
Pfami PF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view ]
PRINTSi PR00792. PEPSIN.
SUPFAMi SSF50630. SSF50630. 1 hit.
PROSITEi PS00141. ASP_PROTEASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: PROTEIN SEQUENCE OF 64-351 AND 354-401.
  2. "Complete amino acid sequence and maturation of the mouse submaxillary gland renin precursor."
    Panthier J.-J., Foote S., Chambraud B., Strosberg A.D., Corvol P., Rougeon F.
    Nature 298:90-92(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  4. "Mouse kidney and submaxillary gland renin genes differ in their 5' putative regulatory sequences."
    Panthier J.-J., Dreyfus M., Roux D.T.L., Rougeon F.
    Proc. Natl. Acad. Sci. U.S.A. 81:5489-5493(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
  5. "The nucleotide sequence of a mouse renin-encoding gene, Ren-1d, and its upstream region."
    Burt D.W., Mullins L.J., George H., Smith G., Brooks J., Pioli D., Brammar W.J.
    Gene 84:91-104(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
  6. "Expression of tissue-specific Ren-1 and Ren-2 genes of mice: comparative analysis of 5'-proximal flanking regions."
    Field L.J., Philbrick W.M., Howles P.N., Dickinson D.P., McGowan R.A., Gross K.W.
    Mol. Cell. Biol. 4:2321-2331(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-30.
  7. "Kidney and submaxillary gland renins are encoded by two non-allelic genes in Swiss mice."
    Panthier J.J., Rougeon F.
    EMBO J. 2:675-678(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 267-292.
  8. Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Entry informationi

Entry nameiRENI2_MOUSE
AccessioniPrimary (citable) accession number: P00796
Secondary accession number(s): P70229, P97955, Q62155
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The active enzyme isolated from the submandibular gland has catalytic and antigenic activities similar to renal renin.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi