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Protein

Chymosin

Gene

CYM

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chymosin is synthesized in the mucosa of the abomasum (fourth stomach) of young (unweaned) ruminants. The enzyme hydrolyzes casein to paracasein.

Catalytic activityi

Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 104-Ser-Phe-|-Met-Ala-107 bond in kappa-chain of casein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92PROSITE-ProRule annotation2 Publications1
Active sitei274PROSITE-ProRule annotation2 Publications1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Biological processi

Digestion

Enzyme and pathway databases

BRENDAi3.4.23.4. 908.
SABIO-RKP00794.

Protein family/group databases

MEROPSiA01.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymosin (EC:3.4.23.4)
Alternative name(s):
Preprorennin
Gene namesi
Name:CYM
Synonyms:CPC
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi308C → D: Propeptide is not cleaved. 1 Publication1
Mutagenesisi341C → S: Propeptide is not cleaved. 1 Publication1

Protein family/group databases

Allergomei3882. Bos d Chymosin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 163 PublicationsAdd BLAST16
PropeptideiPRO_000002598817 – 58Activation peptide1 PublicationAdd BLAST42
ChainiPRO_000002598959 – 381ChymosinAdd BLAST323

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi105 ↔ 1101 Publication
Disulfide bondi265 ↔ 2691 Publication
Disulfide bondi308 ↔ 3411 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP00794.
PRIDEiP00794.

Expressioni

Gene expression databases

BgeeiENSBTAG00000010565.
ExpressionAtlasiP00794. differential.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013970.

Structurei

Secondary structure

1381
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi62 – 65Combined sources4
Beta strandi67 – 69Combined sources3
Turni70 – 72Combined sources3
Beta strandi73 – 80Combined sources8
Turni81 – 84Combined sources4
Beta strandi85 – 92Combined sources8
Beta strandi98 – 100Combined sources3
Helixi108 – 111Combined sources4
Helixi118 – 120Combined sources3
Beta strandi125 – 134Combined sources10
Beta strandi139 – 151Combined sources13
Beta strandi154 – 166Combined sources13
Turni171 – 173Combined sources3
Beta strandi176 – 182Combined sources7
Helixi186 – 188Combined sources3
Helixi196 – 202Combined sources7
Beta strandi206 – 214Combined sources9
Beta strandi217 – 220Combined sources4
Beta strandi223 – 227Combined sources5
Helixi231 – 233Combined sources3
Beta strandi234 – 242Combined sources9
Turni246 – 249Combined sources4
Beta strandi250 – 253Combined sources4
Beta strandi255 – 258Combined sources4
Beta strandi261 – 264Combined sources4
Beta strandi269 – 273Combined sources5
Beta strandi279 – 283Combined sources5
Helixi284 – 294Combined sources11
Beta strandi297 – 299Combined sources3
Turni300 – 302Combined sources3
Beta strandi304 – 306Combined sources3
Helixi308 – 313Combined sources6
Beta strandi317 – 321Combined sources5
Beta strandi324 – 328Combined sources5
Helixi330 – 333Combined sources4
Beta strandi334 – 337Combined sources4
Beta strandi340 – 350Combined sources11
Beta strandi354 – 356Combined sources3
Helixi358 – 361Combined sources4
Beta strandi364 – 369Combined sources6
Turni370 – 373Combined sources4
Beta strandi374 – 380Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CMSX-ray2.30A59-381[»]
1CZIX-ray2.30E59-381[»]
3CMSX-ray2.00A59-381[»]
4AA8X-ray1.80A59-381[»]
4AUCX-ray1.60A59-381[»]
4CMSX-ray2.20A59-381[»]
ProteinModelPortaliP00794.
SMRiP00794.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00794.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 378Peptidase A1PROSITE-ProRule annotationAdd BLAST305

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiP00794.
KOiK01378.
OMAiYFGKIYI.
OrthoDBiEOG091G0JP7.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033142. Chymosin.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PTHR13683:SF294. PTHR13683:SF294. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00794-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCLVVLLAV FALSQGAEIT RIPLYKGKSL RKALKEHGLL EDFLQKQQYG
60 70 80 90 100
ISSKYSGFGE VASVPLTNYL DSQYFGKIYL GTPPQEFTVL FDTGSSDFWV
110 120 130 140 150
PSIYCKSNAC KNHQRFDPRK SSTFQNLGKP LSIHYGTGSM QGILGYDTVT
160 170 180 190 200
VSNIVDIQQT VGLSTQEPGD VFTYAEFDGI LGMAYPSLAS EYSIPVFDNM
210 220 230 240 250
MNRHLVAQDL FSVYMDRNGQ ESMLTLGAID PSYYTGSLHW VPVTVQQYWQ
260 270 280 290 300
FTVDSVTISG VVVACEGGCQ AILDTGTSKL VGPSSDILNI QQAIGATQNQ
310 320 330 340 350
YGEFDIDCDN LSYMPTVVFE INGKMYPLTP SAYTSQDQGF CTSGFQSENH
360 370 380
SQKWILGDVF IREYYSVFDR ANNLVGLAKA I
Length:381
Mass (Da):42,180
Last modified:February 1, 2005 - v3
Checksum:iA4425013B4E2E56A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17A → T in AAA30446 (PubMed:3091454).Curated1
Sequence conflicti109A → G in AAA30446 (PubMed:3091454).Curated1
Sequence conflicti155V → L in AAA30447 (PubMed:6183168).Curated1
Sequence conflicti174Y → T AA sequence (PubMed:329280).Curated1
Sequence conflicti218N → D AA sequence (PubMed:329280).Curated1
Sequence conflicti218N → D AA sequence (PubMed:381305).Curated1
Sequence conflicti230D → N in AAA30448 (PubMed:6283469).Curated1
Sequence conflicti232S → C in AAA30447 (PubMed:6183168).Curated1
Sequence conflicti252T → Y AA sequence (PubMed:381305).Curated1
Sequence conflicti325M → I (PubMed:6804449).Curated1
Sequence conflicti335S → G (PubMed:6804449).Curated1
Sequence conflicti343S → T (PubMed:6804449).Curated1
Sequence conflicti380A → T (PubMed:6804449).Curated1

Polymorphismi

Forms A and B are probably allelic variants. Form B is the predominant form and differs only in one position.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti302G → D in chymosin A. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00002 mRNA. Translation: AAA30447.1.
J00003 mRNA. Translation: AAA30448.1.
M14077
, M14069, M14070, M14071, M14072, M14073, M14074, M14075 Genomic DNA. Translation: AAA30446.1.
EF541122 mRNA. Translation: ABU41411.1.
PIRiA25631. CMBO.
RefSeqiNP_851337.1. NM_180994.2.
UniGeneiBt.394.

Genome annotation databases

EnsembliENSBTAT00000013970; ENSBTAP00000013970; ENSBTAG00000010565.
GeneIDi529879.
KEGGibta:529879.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00002 mRNA. Translation: AAA30447.1.
J00003 mRNA. Translation: AAA30448.1.
M14077
, M14069, M14070, M14071, M14072, M14073, M14074, M14075 Genomic DNA. Translation: AAA30446.1.
EF541122 mRNA. Translation: ABU41411.1.
PIRiA25631. CMBO.
RefSeqiNP_851337.1. NM_180994.2.
UniGeneiBt.394.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CMSX-ray2.30A59-381[»]
1CZIX-ray2.30E59-381[»]
3CMSX-ray2.00A59-381[»]
4AA8X-ray1.80A59-381[»]
4AUCX-ray1.60A59-381[»]
4CMSX-ray2.20A59-381[»]
ProteinModelPortaliP00794.
SMRiP00794.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013970.

Protein family/group databases

Allergomei3882. Bos d Chymosin.
MEROPSiA01.006.

Proteomic databases

PaxDbiP00794.
PRIDEiP00794.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000013970; ENSBTAP00000013970; ENSBTAG00000010565.
GeneIDi529879.
KEGGibta:529879.

Organism-specific databases

CTDi229697.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiP00794.
KOiK01378.
OMAiYFGKIYI.
OrthoDBiEOG091G0JP7.
TreeFamiTF314990.

Enzyme and pathway databases

BRENDAi3.4.23.4. 908.
SABIO-RKP00794.

Miscellaneous databases

EvolutionaryTraceiP00794.

Gene expression databases

BgeeiENSBTAG00000010565.
ExpressionAtlasiP00794. differential.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033142. Chymosin.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PTHR13683:SF294. PTHR13683:SF294. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHYM_BOVIN
AccessioniPrimary (citable) accession number: P00794
Secondary accession number(s): A8RRP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 2005
Last modified: November 30, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.