Reviewed, UniProtKB/Swiss-Prot P00793 (PEPA_CHICK)

Names and origin

Protein names

Recommended name:
    Pepsin A
    EC=3.4.23.1

Gene names

Name:

PGA

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Catalytic activity	Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Biological process	Digestion
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 42	42	Activation peptide		PRO_0000026046
	Chain	43 – 367	325	Pepsin A		PRO_0000026047
Sites
	Active site	77	1
	Active site	260	1
Amino acid modifications
	Glycosylation	113	1	N-linked (GlcNAc...)
	Disulfide bond	90 ↔ 95
	Disulfide bond	251 ↔ 255
	Disulfide bond	290 ↔ 323

Sequences

	Sequence		Length	Mass (Da)
	P00793-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 0C547E7FD8F5B341	FASTA	367	40,432
	10 20 30 40 50 60 SIHRVPLKKG KSLRKQLKDH GLLEDFLKKH PYNPASKYHP VLTATESYEP MTNYMDASYY 70 80 90 100 110 120 GTISIGTPQQ DFSVIFDTGS SNLWVPSIYC KSSACSNHKR FDPSKSSTYV STNETVYIAY 130 140 150 160 170 180 GTGSMSGILG YDTVAVSSID VQNQIFGLSE TEPGSFFYYC NFDGILGLAF PSISSSGATP 190 200 210 220 230 240 VFDNMMSQHL VAQDLFSVYL SKDGETGSFV LFGGIDPNYT TKGIYWVPLS AETYWQITMD 250 260 270 280 290 300 RVTVGNKYVA CFFTCQAIVD TGTSLLVMPQ GAYNRIIKDL GVSSDGEISC DDISKLPDVT 310 320 330 340 350 360 FHINGHAFTL PASAYVLNED GSCMLGFENM GTPTELGEQW ILGDVFIREY YVIFDRANNK VGLSPLS

References

[1]	"Covalent structure of chicken pepsinogen." Baudys M., Kostka V. Eur. J. Biochem. 136:89-99(1983) [PubMed: 6617663] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
IPI	IPI00596079.
UniGene	Gga.402
3D structure databases
HSSP	HSSP built from PDB template 4CMS based on UniProtKB P00794.
ModBase	Search...
Genome annotation databases
Ensembl	ENSGALG00000024059. Gallus gallus. [Contig view]
Phylogenomic databases
HOVERGEN	P00793.
Enzyme and pathway databases
BRENDA	3.4.23.1. 4.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEPA_CHICK

Accession

Primary (citable) accession number: P00793

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)