Reviewed, UniProtKB/Swiss-Prot P00792 (PEPA_BOVIN)

Names and origin

Protein names

Recommended name:
    Pepsin A
    EC=3.4.23.1

Gene names

Name:

PGA

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Catalytic activity	Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 42	42	Activation peptide		PRO_0000026007
	Chain	43 – 372	330	Pepsin A		PRO_0000026008
Sites
	Active site	78	1	By similarity Ref.3
	Active site	261	1	By similarity
Amino acid modifications
	Disulfide bond	91 ↔ 96		Ref.3
	Disulfide bond	252 ↔ 256		By similarity
	Disulfide bond	295 ↔ 328		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00792-1 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: 987D573394E79C34	FASTA	372	40,003
	10 20 30 40 50 60 MSVVKIPLVK KKSLRQNLIE NGKLKEFMRT HKYNLGSKYI REAATLVSEQ PLQNYLDTEY 70 80 90 100 110 120 FGTIGIGTPA QDFTVIFDTG SSNLWVPSIY CSSEACTNHN RFNPQDSSTY EATSETLSIT 130 140 150 160 170 180 YGTGSMTGIL GYDTVQVGGI SDTNQIFGLS ETEPGSFLYY APFDGILGLA YPSISSSGAT 190 200 210 220 230 240 PVFDNIWDQG LVSQDLFSVY LSSNEESGSV VIFGDIDSSY YSGSLNWVPV SVEGYWQITV 250 260 270 280 290 300 DSITMNGESI ACSDGCQAIV DTGTSLLAGP TTAISNIQSY IGASEDSSGE VVISCSSIDS 310 320 330 340 350 360 LPDIVFTING VQYPVPPSAY ILQSNGICSS GFEGMDISTS SGDLWILGDV FIRQYFTVFD 370 RGNNQIGLAP VA

References

[1]	"Cloning of the authentic bovine gene encoding pepsinogen A and its expression in microbial cells." Munoz R., Garcia J.L., Carrascosa A.V., Gonzalez R. Appl. Environ. Microbiol. 70:2588-2595(2004) [PubMed: 15128507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The activation of bovine pepsinogen. Sequence of the peptides released, identification of a pepsin inhibitor." Harboe M.K., Andersen P.M., Foltmann B., Kay J., Kassell B. J. Biol. Chem. 249:4487-4494(1974) [PubMed: 4603017] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-48.
[3]	"Bovine pepsin: the sequence of the first 65 amino acid residues (completing the sequence of the first 110 residues of bovine pepsinogen)." Harboe M.K., Foltmann B. FEBS Lett. 60:133-136(1975) [PubMed: 776669] [Abstract] Cited for: PROTEIN SEQUENCE OF 42-111.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY330769 mRNA. Translation: AAQ95219.1.
IPI	IPI00707862.
PIR	PEBO. A92157.
RefSeq	NP_001001600.1.
UniGene	Bt.15850
3D structure databases
HSSP	HSSP built from PDB template 1PSN based on UniProtKB P00790.
SMR	P00792. Positions 3-372.
ModBase	Search...
Protein family/group databases
MEROPS	A01.001.
Genome annotation databases
Ensembl	ENSBTAG00000014761. Bos taurus. [Contig view]
GeneID	414350.
KEGG	bta:414350.
Phylogenomic databases
HOVERGEN	P00792.
Enzyme and pathway databases
BRENDA	3.4.23.1. 251.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEPA_BOVIN

Accession

Primary (citable) accession number: P00792
Secondary accession number(s): Q6VRA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 16, 2005
Last modified:	June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)