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Protein

Pepsin A

Gene

PGA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Catalytic activityi

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781PROSITE-ProRule annotation
Active sitei261 – 2611PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiA01.070.

Names & Taxonomyi

Protein namesi
Recommended name:
Pepsin A (EC:3.4.23.1)
Gene namesi
Name:PGA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 4242Activation peptidePRO_0000026007Add
BLAST
Chaini43 – 372330Pepsin APRO_0000026008Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi91 ↔ 961 Publication
Disulfide bondi252 ↔ 256By similarity
Disulfide bondi295 ↔ 328By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP00792.
PRIDEiP00792.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019640.

Structurei

3D structure databases

ProteinModelPortaliP00792.
SMRiP00792. Positions 3-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 369310Peptidase A1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOVERGENiHBG000482.
InParanoidiP00792.
KOiK06002.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00792-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVVKIPLVK KKSLRQNLIE NGKLKEFMRT HKYNLGSKYI REAATLVSEQ
60 70 80 90 100
PLQNYLDTEY FGTIGIGTPA QDFTVIFDTG SSNLWVPSIY CSSEACTNHN
110 120 130 140 150
RFNPQDSSTY EATSETLSIT YGTGSMTGIL GYDTVQVGGI SDTNQIFGLS
160 170 180 190 200
ETEPGSFLYY APFDGILGLA YPSISSSGAT PVFDNIWDQG LVSQDLFSVY
210 220 230 240 250
LSSNEESGSV VIFGDIDSSY YSGSLNWVPV SVEGYWQITV DSITMNGESI
260 270 280 290 300
ACSDGCQAIV DTGTSLLAGP TTAISNIQSY IGASEDSSGE VVISCSSIDS
310 320 330 340 350
LPDIVFTING VQYPVPPSAY ILQSNGICSS GFEGMDISTS SGDLWILGDV
360 370
FIRQYFTVFD RGNNQIGLAP VA
Length:372
Mass (Da):40,003
Last modified:August 16, 2005 - v2
Checksum:i987D573394E79C34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY330769 mRNA. Translation: AAQ95219.1.
PIRiA92157. PEBO.
RefSeqiNP_001001600.2. NM_001001600.2.
UniGeneiBt.15850.

Genome annotation databases

GeneIDi414350.
KEGGibta:414350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY330769 mRNA. Translation: AAQ95219.1.
PIRiA92157. PEBO.
RefSeqiNP_001001600.2. NM_001001600.2.
UniGeneiBt.15850.

3D structure databases

ProteinModelPortaliP00792.
SMRiP00792. Positions 3-372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019640.

Protein family/group databases

MEROPSiA01.070.

Proteomic databases

PaxDbiP00792.
PRIDEiP00792.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi414350.
KEGGibta:414350.

Organism-specific databases

CTDi5222.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOVERGENiHBG000482.
InParanoidiP00792.
KOiK06002.

Miscellaneous databases

NextBioi20818705.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of the authentic bovine gene encoding pepsinogen A and its expression in microbial cells."
    Munoz R., Garcia J.L., Carrascosa A.V., Gonzalez R.
    Appl. Environ. Microbiol. 70:2588-2595(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The activation of bovine pepsinogen. Sequence of the peptides released, identification of a pepsin inhibitor."
    Harboe M.K., Andersen P.M., Foltmann B., Kay J., Kassell B.
    J. Biol. Chem. 249:4487-4494(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-48.
  3. "Bovine pepsin: the sequence of the first 65 amino acid residues (completing the sequence of the first 110 residues of bovine pepsinogen)."
    Harboe M.K., Foltmann B.
    FEBS Lett. 60:133-136(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 42-111.

Entry informationi

Entry nameiPEPA_BOVIN
AccessioniPrimary (citable) accession number: P00792
Secondary accession number(s): Q6VRA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 16, 2005
Last modified: April 13, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.