Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pepsin A

Gene

PGA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Catalytic activityi

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78PROSITE-ProRule annotation1
Active sitei261PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
Biological processDigestion

Protein family/group databases

MEROPSiA01.001

Names & Taxonomyi

Protein namesi
Recommended name:
Pepsin A (EC:3.4.23.1)
Gene namesi
Name:PGA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000260071 – 42Activation peptideAdd BLAST42
ChainiPRO_000002600843 – 372Pepsin AAdd BLAST330

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi91 ↔ 961 Publication
Modified residuei114PhosphoserineBy similarity1
Disulfide bondi252 ↔ 256By similarity
Disulfide bondi295 ↔ 328By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

PaxDbiP00792
PRIDEiP00792

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019640

Structurei

3D structure databases

ProteinModelPortaliP00792
SMRiP00792
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 369Peptidase A1PROSITE-ProRule annotationAdd BLAST310

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
HOVERGENiHBG000482
InParanoidiP00792
KOiK06002

Family and domain databases

CDDicd05478 pepsin_A, 1 hit
Gene3Di2.40.70.102 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR012848 Aspartic_peptidase_N
IPR034162 Pepsin_A
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF07966 A1_Propeptide, 1 hit
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00792-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVVKIPLVK KKSLRQNLIE NGKLKEFMRT HKYNLGSKYI REAATLVSEQ
60 70 80 90 100
PLQNYLDTEY FGTIGIGTPA QDFTVIFDTG SSNLWVPSIY CSSEACTNHN
110 120 130 140 150
RFNPQDSSTY EATSETLSIT YGTGSMTGIL GYDTVQVGGI SDTNQIFGLS
160 170 180 190 200
ETEPGSFLYY APFDGILGLA YPSISSSGAT PVFDNIWDQG LVSQDLFSVY
210 220 230 240 250
LSSNEESGSV VIFGDIDSSY YSGSLNWVPV SVEGYWQITV DSITMNGESI
260 270 280 290 300
ACSDGCQAIV DTGTSLLAGP TTAISNIQSY IGASEDSSGE VVISCSSIDS
310 320 330 340 350
LPDIVFTING VQYPVPPSAY ILQSNGICSS GFEGMDISTS SGDLWILGDV
360 370
FIRQYFTVFD RGNNQIGLAP VA
Length:372
Mass (Da):40,003
Last modified:August 16, 2005 - v2
Checksum:i987D573394E79C34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY330769 mRNA Translation: AAQ95219.1
PIRiA92157 PEBO
RefSeqiNP_001001600.2, NM_001001600.2
UniGeneiBt.15850

Genome annotation databases

GeneIDi414350
KEGGibta:414350

Similar proteinsi

Entry informationi

Entry nameiPEPA_BOVIN
AccessioniPrimary (citable) accession number: P00792
Secondary accession number(s): Q6VRA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 16, 2005
Last modified: February 28, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome