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Protein

Calpain-1 catalytic subunit

Gene
N/A
Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Caution

This protein was previously thought to be M-calpain but has since been found to be an intermediate form between the M and Mu types.Curated

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+Note: Binds 3 Ca2+ ions.

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei108By similarity1
Active sitei265By similarity1
Active sitei289By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi545 – 55611 PublicationAdd BLAST12
Calcium bindingi589 – 60021 PublicationAdd BLAST12
Calcium bindingi619 – 63031 PublicationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-GGA-1474228 Degradation of the extracellular matrix

Protein family/group databases

MEROPSiC02.003

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-1 catalytic subunit (EC:3.4.22.52)
Alternative name(s):
Calcium-activated neutral proteinase
Short name:
CANP
Calpain-1 large subunit
Mu/M-type
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3147

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002077011 – 705Calpain-1 catalytic subunitAdd BLAST705

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP00789
PRIDEiP00789

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSGALG00000010186

Interactioni

Subunit structurei

Heterodimer of large (catalytic) and a small (regulatory) subunit.

Protein-protein interaction databases

STRINGi9031.ENSGALP00000016538

Chemistry databases

BindingDBiP00789

Structurei

3D structure databases

ProteinModelPortaliP00789
SMRiP00789
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 347Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini530 – 565EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini606 – 641EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini671 – 705EF-hand 3PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni348 – 517Domain IIIAdd BLAST170
Regioni518 – 533LinkerAdd BLAST16
Regioni534 – 704Domain IVAdd BLAST171

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045 Eukaryota
ENOG410XP0B LUCA
GeneTreeiENSGT00760000118971
HOGENOMiHOG000232035
HOVERGENiHBG012645
InParanoidiP00789
KOiK01367
OMAiHTIGFAV
OrthoDBiEOG091G049E
PhylomeDBiP00789
TreeFamiTF314748

Family and domain databases

CDDicd00214 Calpain_III, 1 hit
cd00044 CysPc, 1 hit
InterProiView protein in InterPro
IPR033883 C2_III
IPR022684 Calpain_cysteine_protease
IPR022682 Calpain_domain_III
IPR022683 Calpain_III
IPR036213 Calpain_III_sf
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR001300 Peptidase_C2_calpain_cat
PfamiView protein in Pfam
PF01067 Calpain_III, 1 hit
PF00648 Peptidase_C2, 1 hit
PRINTSiPR00704 CALPAIN
SMARTiView protein in SMART
SM00720 calpain_III, 1 hit
SM00230 CysPc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF49758 SSF49758, 1 hit
SSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50203 CALPAIN_CAT, 1 hit
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 2 hits
PS00139 THIOL_PROTEASE_CYS, 1 hit

Sequencei

Sequence statusi: Complete.

P00789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMPFGGIAAR LQRDRLRAEG VGEHNNAVKY LNQDYEALKQ ECIESGTLFR
60 70 80 90 100
DPQFPAGPTA LGFKELGPYS SKTRGVEWKR PSELVDDPQF IVGGATRTDI
110 120 130 140 150
CQGALGDCWL LAAIGSLTLN EELLHRVVPH GQSFQEDYAG IFHFQIWQFG
160 170 180 190 200
EWVDVVVDDL LPTKDGELLF VHSAECTEFW SALLEKAYAK LNGCYESLSG
210 220 230 240 250
GSTTEGFEDF TGGVAEMYDL KRAPRNMGHI IRKALERGSL LGCSIDITSA
260 270 280 290 300
FDMEAVTFKK LVKGHAYSVT AFKDVNYRGQ QEQLIRIRNP WGQVEWTGAW
310 320 330 340 350
SDGSSEWDNI DPSDREELQL KMEDGEFWMS FRDFMREFSR LEICNLTPDA
360 370 380 390 400
LTKDELSRWH TQVFEGTWRR GSTAGGCRNN PATFWINPQF KIKLLEEDDD
410 420 430 440 450
PGDDEVACSF LVALMQKHRR RERRVGGDMH TIGFAVYEVP EEAQGSQNVH
460 470 480 490 500
LKKDFFLRNQ SRARSETFIN LREVSNQIRL PPGEYIVVPS TFEPHKEADF
510 520 530 540 550
ILRVFTEKQS DTAELDEEIS ADLADEEEIT EDDIEDGFKN MFQQLAGEDM
560 570 580 590 600
EISVFELKTI LNRVIARHKD LKTDGFSLDS CRNMVNLMDK DGSARLGLVE
610 620 630 640 650
FQILWNKIRS WLTIFRQYDL DKSGTMSSYE MRMALESAGF KLNNKLHQVV
660 670 680 690 700
VARYADAETG VDFDNFVCCL VKLETMFRFF HSMDRDGTGT AVMNLAEWLL

LTMCG
Length:705
Mass (Da):80,352
Last modified:December 1, 2000 - v2
Checksum:iABCDDC56298E48AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01415 mRNA Translation: CAA25658.1
PIRiA00979 CICHH
RefSeqiNP_990634.1, NM_205303.1
XP_015139111.1, XM_015283625.1
UniGeneiGga.1707

Genome annotation databases

EnsembliENSGALT00000075282; ENSGALP00000052109; ENSGALG00000010186
GeneIDi396240
KEGGigga:396240

Similar proteinsi

Entry informationi

Entry nameiCANX_CHICK
AccessioniPrimary (citable) accession number: P00789
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 1, 2000
Last modified: June 20, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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