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P00789

- CANX_CHICK

UniProt

P00789 - CANX_CHICK

Protein

Calpain-1 catalytic subunit

Gene
N/A
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

    Catalytic activityi

    Broad endopeptidase specificity.

    Cofactori

    Binds 3 calcium ions.

    Enzyme regulationi

    Activated by micromolar concentrations of calcium and inhibited by calpastatin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei108 – 1081By similarity
    Active sitei265 – 2651By similarity
    Active sitei289 – 2891By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi545 – 556121Add
    BLAST
    Calcium bindingi589 – 600122Add
    BLAST
    Calcium bindingi619 – 630123Add
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: RefGenome
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. proteolysis Source: RefGenome
    2. receptor catabolic process Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    MEROPSiC02.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calpain-1 catalytic subunit (EC:3.4.22.52)
    Alternative name(s):
    Calcium-activated neutral proteinase
    Short name:
    CANP
    Calpain-1 large subunit
    Mu/M-type
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Chromosome 3

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity
    Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 705705Calpain-1 catalytic subunitPRO_0000207701Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Proteomic databases

    PaxDbiP00789.
    PRIDEiP00789.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.

    Interactioni

    Subunit structurei

    Heterodimer of large (catalytic) and a small (regulatory) subunit.

    Structurei

    3D structure databases

    ProteinModelPortaliP00789.
    SMRiP00789. Positions 5-703.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini48 – 347300Calpain catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini530 – 56536EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini606 – 64136EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini671 – 70535EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni348 – 517170Domain IIIAdd
    BLAST
    Regioni518 – 53316LinkerAdd
    BLAST
    Regioni534 – 704171Domain IVAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C2 family.Curated
    Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
    Contains 3 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG327523.
    GeneTreeiENSGT00750000117643.
    HOGENOMiHOG000232035.
    HOVERGENiHBG012645.
    InParanoidiP00789.
    KOiK01367.
    OMAiHTIGFAV.
    OrthoDBiEOG7RV9FM.
    PhylomeDBiP00789.
    TreeFamiTF314748.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view]
    PfamiPF01067. Calpain_III. 1 hit.
    PF13405. EF-hand_6. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view]
    PRINTSiPR00704. CALPAIN.
    SMARTiSM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF49758. SSF49758. 1 hit.
    PROSITEiPS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00789-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMPFGGIAAR LQRDRLRAEG VGEHNNAVKY LNQDYEALKQ ECIESGTLFR    50
    DPQFPAGPTA LGFKELGPYS SKTRGVEWKR PSELVDDPQF IVGGATRTDI 100
    CQGALGDCWL LAAIGSLTLN EELLHRVVPH GQSFQEDYAG IFHFQIWQFG 150
    EWVDVVVDDL LPTKDGELLF VHSAECTEFW SALLEKAYAK LNGCYESLSG 200
    GSTTEGFEDF TGGVAEMYDL KRAPRNMGHI IRKALERGSL LGCSIDITSA 250
    FDMEAVTFKK LVKGHAYSVT AFKDVNYRGQ QEQLIRIRNP WGQVEWTGAW 300
    SDGSSEWDNI DPSDREELQL KMEDGEFWMS FRDFMREFSR LEICNLTPDA 350
    LTKDELSRWH TQVFEGTWRR GSTAGGCRNN PATFWINPQF KIKLLEEDDD 400
    PGDDEVACSF LVALMQKHRR RERRVGGDMH TIGFAVYEVP EEAQGSQNVH 450
    LKKDFFLRNQ SRARSETFIN LREVSNQIRL PPGEYIVVPS TFEPHKEADF 500
    ILRVFTEKQS DTAELDEEIS ADLADEEEIT EDDIEDGFKN MFQQLAGEDM 550
    EISVFELKTI LNRVIARHKD LKTDGFSLDS CRNMVNLMDK DGSARLGLVE 600
    FQILWNKIRS WLTIFRQYDL DKSGTMSSYE MRMALESAGF KLNNKLHQVV 650
    VARYADAETG VDFDNFVCCL VKLETMFRFF HSMDRDGTGT AVMNLAEWLL 700
    LTMCG 705
    Length:705
    Mass (Da):80,352
    Last modified:December 1, 2000 - v2
    Checksum:iABCDDC56298E48AA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01415 mRNA. Translation: CAA25658.1.
    PIRiA00979. CICHH.
    RefSeqiNP_990634.1. NM_205303.1.
    UniGeneiGga.1707.

    Genome annotation databases

    EnsembliENSGALT00000016557; ENSGALP00000016538; ENSGALG00000010186.
    GeneIDi396240.
    KEGGigga:396240.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01415 mRNA. Translation: CAA25658.1 .
    PIRi A00979. CICHH.
    RefSeqi NP_990634.1. NM_205303.1.
    UniGenei Gga.1707.

    3D structure databases

    ProteinModelPortali P00789.
    SMRi P00789. Positions 5-703.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P00789.
    ChEMBLi CHEMBL3147.

    Protein family/group databases

    MEROPSi C02.003.

    Proteomic databases

    PaxDbi P00789.
    PRIDEi P00789.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSGALT00000016557 ; ENSGALP00000016538 ; ENSGALG00000010186 .
    GeneIDi 396240.
    KEGGi gga:396240.

    Organism-specific databases

    CTDi 823.

    Phylogenomic databases

    eggNOGi NOG327523.
    GeneTreei ENSGT00750000117643.
    HOGENOMi HOG000232035.
    HOVERGENi HBG012645.
    InParanoidi P00789.
    KOi K01367.
    OMAi HTIGFAV.
    OrthoDBi EOG7RV9FM.
    PhylomeDBi P00789.
    TreeFami TF314748.

    Miscellaneous databases

    NextBioi 20816292.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view ]
    Pfami PF01067. Calpain_III. 1 hit.
    PF13405. EF-hand_6. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view ]
    PRINTSi PR00704. CALPAIN.
    SMARTi SM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49758. SSF49758. 1 hit.
    PROSITEi PS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolutionary origin of a calcium-dependent protease by fusion of genes for a thiol protease and a calcium-binding protein?"
      Ohno S., Emori Y., Imajoh S., Kawasaki H., Kisaragi M., Suzuki K.
      Nature 312:566-570(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Gene structure of calcium-dependent protease retains the ancestral organization of the calcium-binding protein gene."
      Emori Y., Ohno S., Tobita M., Suzuki K.
      FEBS Lett. 194:249-252(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "E-F hand structure-domain of calcium-activated neutral protease (CANP) can bind Ca2+ ions."
      Minami Y., Emori Y., Kawasaki H., Suzuki K.
      J. Biochem. 101:889-895(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: CALCIUM-BINDING DATA.
    4. "Identification of a third ubiquitous calpain species -- chicken muscle expresses four distinct calpains."
      Sorimachi H., Tsukahara T., Okada-Ban M., Sugita H., Ishiura S., Suzuki K.
      Biochim. Biophys. Acta 1261:381-393(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiCANX_CHICK
    AccessioniPrimary (citable) accession number: P00789
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    This protein was previously thought to be M-calpain but has since been found to be an intermediate form between the M and Mu types.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3