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P00789

- CANX_CHICK

UniProt

P00789 - CANX_CHICK

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Protein

Calpain-1 catalytic subunit

Gene
N/A
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Binds 3 calcium ions.

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei108 – 1081By similarity
Active sitei265 – 2651By similarity
Active sitei289 – 2891By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi545 – 556121Add
BLAST
Calcium bindingi589 – 600122Add
BLAST
Calcium bindingi619 – 630123Add
BLAST

GO - Molecular functioni

  1. calcium-dependent cysteine-type endopeptidase activity Source: RefGenome
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. proteolysis Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiC02.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-1 catalytic subunit (EC:3.4.22.52)
Alternative name(s):
Calcium-activated neutral proteinase
Short name:
CANP
Calpain-1 large subunit
Mu/M-type
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome 3

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

  1. acrosomal vesicle Source: Ensembl
  2. cytoplasm Source: RefGenome
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 705705Calpain-1 catalytic subunitPRO_0000207701Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP00789.
PRIDEiP00789.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Interactioni

Subunit structurei

Heterodimer of large (catalytic) and a small (regulatory) subunit.

Structurei

3D structure databases

ProteinModelPortaliP00789.
SMRiP00789. Positions 5-703.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini48 – 347300Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini530 – 56536EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini606 – 64136EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini671 – 70535EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni348 – 517170Domain IIIAdd
BLAST
Regioni518 – 53316LinkerAdd
BLAST
Regioni534 – 704171Domain IVAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG327523.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiP00789.
KOiK01367.
OMAiHTIGFAV.
OrthoDBiEOG7RV9FM.
PhylomeDBiP00789.
TreeFamiTF314748.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF13405. EF-hand_6. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00789-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMPFGGIAAR LQRDRLRAEG VGEHNNAVKY LNQDYEALKQ ECIESGTLFR
60 70 80 90 100
DPQFPAGPTA LGFKELGPYS SKTRGVEWKR PSELVDDPQF IVGGATRTDI
110 120 130 140 150
CQGALGDCWL LAAIGSLTLN EELLHRVVPH GQSFQEDYAG IFHFQIWQFG
160 170 180 190 200
EWVDVVVDDL LPTKDGELLF VHSAECTEFW SALLEKAYAK LNGCYESLSG
210 220 230 240 250
GSTTEGFEDF TGGVAEMYDL KRAPRNMGHI IRKALERGSL LGCSIDITSA
260 270 280 290 300
FDMEAVTFKK LVKGHAYSVT AFKDVNYRGQ QEQLIRIRNP WGQVEWTGAW
310 320 330 340 350
SDGSSEWDNI DPSDREELQL KMEDGEFWMS FRDFMREFSR LEICNLTPDA
360 370 380 390 400
LTKDELSRWH TQVFEGTWRR GSTAGGCRNN PATFWINPQF KIKLLEEDDD
410 420 430 440 450
PGDDEVACSF LVALMQKHRR RERRVGGDMH TIGFAVYEVP EEAQGSQNVH
460 470 480 490 500
LKKDFFLRNQ SRARSETFIN LREVSNQIRL PPGEYIVVPS TFEPHKEADF
510 520 530 540 550
ILRVFTEKQS DTAELDEEIS ADLADEEEIT EDDIEDGFKN MFQQLAGEDM
560 570 580 590 600
EISVFELKTI LNRVIARHKD LKTDGFSLDS CRNMVNLMDK DGSARLGLVE
610 620 630 640 650
FQILWNKIRS WLTIFRQYDL DKSGTMSSYE MRMALESAGF KLNNKLHQVV
660 670 680 690 700
VARYADAETG VDFDNFVCCL VKLETMFRFF HSMDRDGTGT AVMNLAEWLL

LTMCG
Length:705
Mass (Da):80,352
Last modified:December 1, 2000 - v2
Checksum:iABCDDC56298E48AA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01415 mRNA. Translation: CAA25658.1.
PIRiA00979. CICHH.
RefSeqiNP_990634.1. NM_205303.1.
UniGeneiGga.1707.

Genome annotation databases

EnsembliENSGALT00000016557; ENSGALP00000016538; ENSGALG00000010186.
GeneIDi396240.
KEGGigga:396240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01415 mRNA. Translation: CAA25658.1 .
PIRi A00979. CICHH.
RefSeqi NP_990634.1. NM_205303.1.
UniGenei Gga.1707.

3D structure databases

ProteinModelPortali P00789.
SMRi P00789. Positions 5-703.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P00789.
ChEMBLi CHEMBL3147.

Protein family/group databases

MEROPSi C02.003.

Proteomic databases

PaxDbi P00789.
PRIDEi P00789.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSGALT00000016557 ; ENSGALP00000016538 ; ENSGALG00000010186 .
GeneIDi 396240.
KEGGi gga:396240.

Organism-specific databases

CTDi 823.

Phylogenomic databases

eggNOGi NOG327523.
GeneTreei ENSGT00760000118971.
HOGENOMi HOG000232035.
HOVERGENi HBG012645.
InParanoidi P00789.
KOi K01367.
OMAi HTIGFAV.
OrthoDBi EOG7RV9FM.
PhylomeDBi P00789.
TreeFami TF314748.

Miscellaneous databases

NextBioi 20816292.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
Pfami PF01067. Calpain_III. 1 hit.
PF13405. EF-hand_6. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evolutionary origin of a calcium-dependent protease by fusion of genes for a thiol protease and a calcium-binding protein?"
    Ohno S., Emori Y., Imajoh S., Kawasaki H., Kisaragi M., Suzuki K.
    Nature 312:566-570(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Gene structure of calcium-dependent protease retains the ancestral organization of the calcium-binding protein gene."
    Emori Y., Ohno S., Tobita M., Suzuki K.
    FEBS Lett. 194:249-252(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "E-F hand structure-domain of calcium-activated neutral protease (CANP) can bind Ca2+ ions."
    Minami Y., Emori Y., Kawasaki H., Suzuki K.
    J. Biochem. 101:889-895(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: CALCIUM-BINDING DATA.
  4. "Identification of a third ubiquitous calpain species -- chicken muscle expresses four distinct calpains."
    Sorimachi H., Tsukahara T., Okada-Ban M., Sugita H., Ishiura S., Suzuki K.
    Biochim. Biophys. Acta 1261:381-393(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiCANX_CHICK
AccessioniPrimary (citable) accession number: P00789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

This protein was previously thought to be M-calpain but has since been found to be an intermediate form between the M and Mu types.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3