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Protein

Calpain-1 catalytic subunit

Gene
N/A
Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+Note: Binds 3 Ca2+ ions.

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei108By similarity1
Active sitei265By similarity1
Active sitei289By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi545 – 55611 PublicationAdd BLAST12
Calcium bindingi589 – 60021 PublicationAdd BLAST12
Calcium bindingi619 – 63031 PublicationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiC02.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-1 catalytic subunit (EC:3.4.22.52)
Alternative name(s):
Calcium-activated neutral proteinase
Short name:
CANP
Calpain-1 large subunit
Mu/M-type
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 3

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3147.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002077011 – 705Calpain-1 catalytic subunitAdd BLAST705

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP00789.
PRIDEiP00789.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSGALG00000010186.

Interactioni

Subunit structurei

Heterodimer of large (catalytic) and a small (regulatory) subunit.

Protein-protein interaction databases

STRINGi9031.ENSGALP00000016538.

Chemistry databases

BindingDBiP00789.

Structurei

3D structure databases

ProteinModelPortaliP00789.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 347Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini530 – 565EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini606 – 641EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini671 – 705EF-hand 3PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni348 – 517Domain IIIAdd BLAST170
Regioni518 – 533LinkerAdd BLAST16
Regioni534 – 704Domain IVAdd BLAST171

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiP00789.
KOiK01367.
OMAiHTIGFAV.
OrthoDBiEOG091G049E.
PhylomeDBiP00789.
TreeFamiTF314748.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMPFGGIAAR LQRDRLRAEG VGEHNNAVKY LNQDYEALKQ ECIESGTLFR
60 70 80 90 100
DPQFPAGPTA LGFKELGPYS SKTRGVEWKR PSELVDDPQF IVGGATRTDI
110 120 130 140 150
CQGALGDCWL LAAIGSLTLN EELLHRVVPH GQSFQEDYAG IFHFQIWQFG
160 170 180 190 200
EWVDVVVDDL LPTKDGELLF VHSAECTEFW SALLEKAYAK LNGCYESLSG
210 220 230 240 250
GSTTEGFEDF TGGVAEMYDL KRAPRNMGHI IRKALERGSL LGCSIDITSA
260 270 280 290 300
FDMEAVTFKK LVKGHAYSVT AFKDVNYRGQ QEQLIRIRNP WGQVEWTGAW
310 320 330 340 350
SDGSSEWDNI DPSDREELQL KMEDGEFWMS FRDFMREFSR LEICNLTPDA
360 370 380 390 400
LTKDELSRWH TQVFEGTWRR GSTAGGCRNN PATFWINPQF KIKLLEEDDD
410 420 430 440 450
PGDDEVACSF LVALMQKHRR RERRVGGDMH TIGFAVYEVP EEAQGSQNVH
460 470 480 490 500
LKKDFFLRNQ SRARSETFIN LREVSNQIRL PPGEYIVVPS TFEPHKEADF
510 520 530 540 550
ILRVFTEKQS DTAELDEEIS ADLADEEEIT EDDIEDGFKN MFQQLAGEDM
560 570 580 590 600
EISVFELKTI LNRVIARHKD LKTDGFSLDS CRNMVNLMDK DGSARLGLVE
610 620 630 640 650
FQILWNKIRS WLTIFRQYDL DKSGTMSSYE MRMALESAGF KLNNKLHQVV
660 670 680 690 700
VARYADAETG VDFDNFVCCL VKLETMFRFF HSMDRDGTGT AVMNLAEWLL

LTMCG
Length:705
Mass (Da):80,352
Last modified:December 1, 2000 - v2
Checksum:iABCDDC56298E48AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01415 mRNA. Translation: CAA25658.1.
PIRiA00979. CICHH.
RefSeqiNP_990634.1. NM_205303.1.
XP_015139111.1. XM_015283625.1.
UniGeneiGga.1707.

Genome annotation databases

EnsembliENSGALT00000075282; ENSGALP00000052109; ENSGALG00000010186.
GeneIDi396240.
KEGGigga:396240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01415 mRNA. Translation: CAA25658.1.
PIRiA00979. CICHH.
RefSeqiNP_990634.1. NM_205303.1.
XP_015139111.1. XM_015283625.1.
UniGeneiGga.1707.

3D structure databases

ProteinModelPortaliP00789.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000016538.

Chemistry databases

BindingDBiP00789.
ChEMBLiCHEMBL3147.

Protein family/group databases

MEROPSiC02.003.

Proteomic databases

PaxDbiP00789.
PRIDEiP00789.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000075282; ENSGALP00000052109; ENSGALG00000010186.
GeneIDi396240.
KEGGigga:396240.

Organism-specific databases

CTDi11131.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiP00789.
KOiK01367.
OMAiHTIGFAV.
OrthoDBiEOG091G049E.
PhylomeDBiP00789.
TreeFamiTF314748.

Miscellaneous databases

PROiP00789.

Gene expression databases

BgeeiENSGALG00000010186.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCANX_CHICK
AccessioniPrimary (citable) accession number: P00789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

This protein was previously thought to be M-calpain but has since been found to be an intermediate form between the M and Mu types.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.