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P00789

- CANX_CHICK

UniProt

P00789 - CANX_CHICK

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Protein
Calpain-1 catalytic subunit
Gene
N/A
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Binds 3 calcium ions.

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei108 – 1081 By similarity
Active sitei265 – 2651 By similarity
Active sitei289 – 2891 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi545 – 5561211 Publication
Add
BLAST
Calcium bindingi589 – 6001221 Publication
Add
BLAST
Calcium bindingi619 – 6301231 Publication
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. calcium-dependent cysteine-type endopeptidase activity Source: RefGenome

GO - Biological processi

  1. proteolysis Source: RefGenome
  2. receptor catabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiC02.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-1 catalytic subunit (EC:3.4.22.52)
Alternative name(s):
Calcium-activated neutral proteinase
Short name:
CANP
Calpain-1 large subunit
Mu/M-type
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome 3

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding By similarity.

GO - Cellular componenti

  1. cytoplasm Source: RefGenome
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 705705Calpain-1 catalytic subunit
PRO_0000207701Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP00789.
PRIDEiP00789.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Interactioni

Subunit structurei

Heterodimer of large (catalytic) and a small (regulatory) subunit.

Structurei

3D structure databases

ProteinModelPortaliP00789.
SMRiP00789. Positions 5-703.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini48 – 347300Calpain catalytic
Add
BLAST
Domaini530 – 56536EF-hand 1
Add
BLAST
Domaini606 – 64136EF-hand 2
Add
BLAST
Domaini671 – 70535EF-hand 3
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni348 – 517170Domain III
Add
BLAST
Regioni518 – 53316Linker
Add
BLAST
Regioni534 – 704171Domain IV
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.
Contains 3 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG327523.
GeneTreeiENSGT00750000117643.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiP00789.
KOiK01367.
OMAiHTIGFAV.
OrthoDBiEOG7RV9FM.
PhylomeDBiP00789.
TreeFamiTF314748.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF13405. EF-hand_6. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00789-1 [UniParc]FASTAAdd to Basket

« Hide

MMPFGGIAAR LQRDRLRAEG VGEHNNAVKY LNQDYEALKQ ECIESGTLFR    50
DPQFPAGPTA LGFKELGPYS SKTRGVEWKR PSELVDDPQF IVGGATRTDI 100
CQGALGDCWL LAAIGSLTLN EELLHRVVPH GQSFQEDYAG IFHFQIWQFG 150
EWVDVVVDDL LPTKDGELLF VHSAECTEFW SALLEKAYAK LNGCYESLSG 200
GSTTEGFEDF TGGVAEMYDL KRAPRNMGHI IRKALERGSL LGCSIDITSA 250
FDMEAVTFKK LVKGHAYSVT AFKDVNYRGQ QEQLIRIRNP WGQVEWTGAW 300
SDGSSEWDNI DPSDREELQL KMEDGEFWMS FRDFMREFSR LEICNLTPDA 350
LTKDELSRWH TQVFEGTWRR GSTAGGCRNN PATFWINPQF KIKLLEEDDD 400
PGDDEVACSF LVALMQKHRR RERRVGGDMH TIGFAVYEVP EEAQGSQNVH 450
LKKDFFLRNQ SRARSETFIN LREVSNQIRL PPGEYIVVPS TFEPHKEADF 500
ILRVFTEKQS DTAELDEEIS ADLADEEEIT EDDIEDGFKN MFQQLAGEDM 550
EISVFELKTI LNRVIARHKD LKTDGFSLDS CRNMVNLMDK DGSARLGLVE 600
FQILWNKIRS WLTIFRQYDL DKSGTMSSYE MRMALESAGF KLNNKLHQVV 650
VARYADAETG VDFDNFVCCL VKLETMFRFF HSMDRDGTGT AVMNLAEWLL 700
LTMCG 705
Length:705
Mass (Da):80,352
Last modified:December 1, 2000 - v2
Checksum:iABCDDC56298E48AA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01415 mRNA. Translation: CAA25658.1.
PIRiA00979. CICHH.
RefSeqiNP_990634.1. NM_205303.1.
UniGeneiGga.1707.

Genome annotation databases

EnsembliENSGALT00000016557; ENSGALP00000016538; ENSGALG00000010186.
GeneIDi396240.
KEGGigga:396240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01415 mRNA. Translation: CAA25658.1 .
PIRi A00979. CICHH.
RefSeqi NP_990634.1. NM_205303.1.
UniGenei Gga.1707.

3D structure databases

ProteinModelPortali P00789.
SMRi P00789. Positions 5-703.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P00789.
ChEMBLi CHEMBL3147.

Protein family/group databases

MEROPSi C02.003.

Proteomic databases

PaxDbi P00789.
PRIDEi P00789.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSGALT00000016557 ; ENSGALP00000016538 ; ENSGALG00000010186 .
GeneIDi 396240.
KEGGi gga:396240.

Organism-specific databases

CTDi 823.

Phylogenomic databases

eggNOGi NOG327523.
GeneTreei ENSGT00750000117643.
HOGENOMi HOG000232035.
HOVERGENi HBG012645.
InParanoidi P00789.
KOi K01367.
OMAi HTIGFAV.
OrthoDBi EOG7RV9FM.
PhylomeDBi P00789.
TreeFami TF314748.

Miscellaneous databases

NextBioi 20816292.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
Pfami PF01067. Calpain_III. 1 hit.
PF13405. EF-hand_6. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evolutionary origin of a calcium-dependent protease by fusion of genes for a thiol protease and a calcium-binding protein?"
    Ohno S., Emori Y., Imajoh S., Kawasaki H., Kisaragi M., Suzuki K.
    Nature 312:566-570(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Gene structure of calcium-dependent protease retains the ancestral organization of the calcium-binding protein gene."
    Emori Y., Ohno S., Tobita M., Suzuki K.
    FEBS Lett. 194:249-252(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "E-F hand structure-domain of calcium-activated neutral protease (CANP) can bind Ca2+ ions."
    Minami Y., Emori Y., Kawasaki H., Suzuki K.
    J. Biochem. 101:889-895(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: CALCIUM-BINDING DATA.
  4. "Identification of a third ubiquitous calpain species -- chicken muscle expresses four distinct calpains."
    Sorimachi H., Tsukahara T., Okada-Ban M., Sugita H., Ishiura S., Suzuki K.
    Biochim. Biophys. Acta 1261:381-393(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiCANX_CHICK
AccessioniPrimary (citable) accession number: P00789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

This protein was previously thought to be M-calpain but has since been found to be an intermediate form between the M and Mu types.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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