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P00786

- CATH_RAT

UniProt

P00786 - CATH_RAT

Protein

Pro-cathepsin H

Gene

Ctsh

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Important for the overall degradation of proteins in lysosomes.

    Catalytic activityi

    Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei139 – 1391By similarity
    Active sitei279 – 2791By similarity
    Active sitei299 – 2991By similarity

    GO - Molecular functioni

    1. aminopeptidase activity Source: RGD
    2. cysteine-type endopeptidase activator activity involved in apoptotic process Source: UniProtKB
    3. cysteine-type endopeptidase activity Source: UniProtKB
    4. HLA-A specific activating MHC class I receptor activity Source: UniProtKB
    5. kininogen binding Source: RGD
    6. protein complex binding Source: RGD
    7. protein self-association Source: RGD
    8. serine-type endopeptidase activity Source: UniProtKB
    9. thyroid hormone binding Source: UniProtKB

    GO - Biological processi

    1. bradykinin catabolic process Source: UniProtKB
    2. cellular response to thyroid hormone stimulus Source: UniProtKB
    3. dichotomous subdivision of terminal units involved in lung branching Source: UniProtKB
    4. ERK1 and ERK2 cascade Source: UniProtKB
    5. immune response-regulating signaling pathway Source: UniProtKB
    6. membrane protein proteolysis Source: UniProtKB
    7. metanephros development Source: UniProtKB
    8. negative regulation of apoptotic process Source: UniProtKB
    9. neuropeptide catabolic process Source: UniProtKB
    10. positive regulation of angiogenesis Source: UniProtKB
    11. positive regulation of apoptotic signaling pathway Source: Ensembl
    12. positive regulation of cell migration Source: UniProtKB
    13. positive regulation of cell proliferation Source: UniProtKB
    14. positive regulation of epithelial cell migration Source: UniProtKB
    15. positive regulation of gene expression Source: UniProtKB
    16. positive regulation of peptidase activity Source: UniProtKB
    17. protein destabilization Source: UniProtKB
    18. proteolysis Source: RGD
    19. response to retinoic acid Source: UniProtKB
    20. spermatogenesis Source: RGD
    21. surfactant homeostasis Source: UniProtKB
    22. T cell mediated cytotoxicity Source: UniProtKB
    23. zymogen activation Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Protein family/group databases

    MEROPSiC01.040.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pro-cathepsin H
    Cleaved into the following 4 chains:
    Gene namesi
    Name:Ctsh
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi2447. Ctsh.

    Subcellular locationi

    GO - Cellular componenti

    1. acrosomal vesicle Source: RGD
    2. alveolar lamellar body Source: UniProtKB
    3. axoneme Source: RGD
    4. cytosol Source: UniProtKB
    5. extracellular space Source: UniProtKB
    6. lysosome Source: UniProtKB
    7. outer dense fiber Source: RGD

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Propeptidei21 – 9575Activation peptidePRO_0000026224Add
    BLAST
    Chaini96 – 1038Cathepsin H mini chainPRO_0000026225
    Propeptidei104 – 11310Sequence AnalysisPRO_0000026226
    Chaini114 – 333220Cathepsin HPRO_0000026227Add
    BLAST
    Chaini114 – 290177Cathepsin H heavy chainPRO_0000026228Add
    BLAST
    Chaini291 – 33343Cathepsin H light chainPRO_0000026229Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi100 ↔ 325By similarity
    Disulfide bondi136 ↔ 179By similarity
    Disulfide bondi170 ↔ 212By similarity
    Glycosylationi228 – 2281N-linked (GlcNAc...)1 Publication
    Disulfide bondi270 ↔ 320By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP00786.
    PRIDEiP00786.

    Expressioni

    Gene expression databases

    GenevestigatoriP00786.

    Interactioni

    Subunit structurei

    Composed of a mini chain and a large chain. The large chain may be split into heavy and light chain. All chains are held together by disulfide bonds.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000019285.

    Structurei

    3D structure databases

    ProteinModelPortaliP00786.
    SMRiP00786. Positions 114-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4870.
    GeneTreeiENSGT00750000117440.
    HOGENOMiHOG000230774.
    HOVERGENiHBG011513.
    InParanoidiP00786.
    KOiK01366.
    OMAiHKYLWSE.
    OrthoDBiEOG70KGPX.
    PhylomeDBiP00786.
    TreeFamiTF328985.

    Family and domain databases

    InterProiIPR025661. Pept_asp_AS.
    IPR000169. Pept_cys_AS.
    IPR025660. Pept_his_AS.
    IPR013128. Peptidase_C1A.
    IPR000668. Peptidase_C1A_C.
    IPR013201. Prot_inhib_I29.
    [Graphical view]
    PANTHERiPTHR12411. PTHR12411. 1 hit.
    PfamiPF08246. Inhibitor_I29. 1 hit.
    PF00112. Peptidase_C1. 1 hit.
    [Graphical view]
    PRINTSiPR00705. PAPAIN.
    SMARTiSM00848. Inhibitor_I29. 1 hit.
    SM00645. Pept_C1. 1 hit.
    [Graphical view]
    PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    PS00639. THIOL_PROTEASE_HIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00786-1 [UniParc]FASTAAdd to Basket

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    MWTALPLLCA GAWLLSAGAT AELTVNAIEK FHFTSWMKQH QKTYSSREYS    50
    HRLQVFANNW RKIQAHNQRN HTFKMGLNQF SDMSFAEIKH KYLWSEPQNC 100
    SATKSNYLRG TGPYPSSMDW RKKGNVVSPV KNQGACGSCW TFSTTGALES 150
    AVAIASGKMM TLAEQQLVDC AQNFNNHGCQ GGLPSQAFEY ILYNKGIMGE 200
    DSYPYIGKNG QCKFNPEKAV AFVKNVVNIT LNDEAAMVEA VALYNPVSFA 250
    FEVTEDFMMY KSGVYSSNSC HKTPDKVNHA VLAVGYGEQN GLLYWIVKNS 300
    WGSNWGNNGY FLIERGKNMC GLAACASYPI PQV 333
    Length:333
    Mass (Da):37,104
    Last modified:August 1, 1988 - v1
    Checksum:iA90AC66338F0B744
    GO

    Sequence cautioni

    The sequence AAA63484.1 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti287 – 2871G → A.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00708 mRNA. Translation: CAA68699.1.
    BC085352 mRNA. Translation: AAH85352.1.
    M38135 mRNA. Translation: AAA63484.1. Different initiation.
    PIRiS00211. KHRTH.
    RefSeqiNP_037071.1. NM_012939.1.
    UniGeneiRn.1997.

    Genome annotation databases

    EnsembliENSRNOT00000019285; ENSRNOP00000019285; ENSRNOG00000014064.
    GeneIDi25425.
    KEGGirno:25425.
    UCSCiRGD:2447. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00708 mRNA. Translation: CAA68699.1 .
    BC085352 mRNA. Translation: AAH85352.1 .
    M38135 mRNA. Translation: AAA63484.1 . Different initiation.
    PIRi S00211. KHRTH.
    RefSeqi NP_037071.1. NM_012939.1.
    UniGenei Rn.1997.

    3D structure databases

    ProteinModelPortali P00786.
    SMRi P00786. Positions 114-333.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000019285.

    Chemistry

    BindingDBi P00786.

    Protein family/group databases

    MEROPSi C01.040.

    Proteomic databases

    PaxDbi P00786.
    PRIDEi P00786.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000019285 ; ENSRNOP00000019285 ; ENSRNOG00000014064 .
    GeneIDi 25425.
    KEGGi rno:25425.
    UCSCi RGD:2447. rat.

    Organism-specific databases

    CTDi 1512.
    RGDi 2447. Ctsh.

    Phylogenomic databases

    eggNOGi COG4870.
    GeneTreei ENSGT00750000117440.
    HOGENOMi HOG000230774.
    HOVERGENi HBG011513.
    InParanoidi P00786.
    KOi K01366.
    OMAi HKYLWSE.
    OrthoDBi EOG70KGPX.
    PhylomeDBi P00786.
    TreeFami TF328985.

    Miscellaneous databases

    NextBioi 606599.
    PROi P00786.

    Gene expression databases

    Genevestigatori P00786.

    Family and domain databases

    InterProi IPR025661. Pept_asp_AS.
    IPR000169. Pept_cys_AS.
    IPR025660. Pept_his_AS.
    IPR013128. Peptidase_C1A.
    IPR000668. Peptidase_C1A_C.
    IPR013201. Prot_inhib_I29.
    [Graphical view ]
    PANTHERi PTHR12411. PTHR12411. 1 hit.
    Pfami PF08246. Inhibitor_I29. 1 hit.
    PF00112. Peptidase_C1. 1 hit.
    [Graphical view ]
    PRINTSi PR00705. PAPAIN.
    SMARTi SM00848. Inhibitor_I29. 1 hit.
    SM00645. Pept_C1. 1 hit.
    [Graphical view ]
    PROSITEi PS00640. THIOL_PROTEASE_ASN. 1 hit.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    PS00639. THIOL_PROTEASE_HIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and sequencing of cDNA for rat cathepsin H. Homology in pro-peptide regions of cysteine proteinases."
      Ishidoh K., Imajoh S., Emori Y., Ohno S., Kawasaki H., Minami Y., Kominami E., Katunuma N., Suzuki K.
      FEBS Lett. 226:33-37(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Kidney.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    4. "Molecular cloning of rat precursor cathepsin H and the expression of five lysosomal cathepsins in normal tissues and in a rat carcinosarcoma."
      Qian F., Frankfater A., Miller R.V., Chan S.J., Steiner D.F.
      Int. J. Biochem. 22:1457-1464(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-333.
      Tissue: Liver.
    5. "Homology of amino acid sequences of rat liver cathepsins B and H with that of papain."
      Takio K., Towatari T., Katunuma N., Teller D.C., Titani K.
      Proc. Natl. Acad. Sci. U.S.A. 80:3666-3670(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 114-333.
      Tissue: Liver.

    Entry informationi

    Entry nameiCATH_RAT
    AccessioniPrimary (citable) accession number: P00786
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3