Reviewed, UniProtKB/Swiss-Prot P00786 (CATH_RAT)

Names and origin

Protein names

Recommended name:
    Cathepsin H
    EC=3.4.22.16
Alternative name(s):
    Cathepsin B3
    Cathepsin BA
Cleaved into the following 3 chains:
    1- Recommended name:
            Cathepsin H mini chain
    2- Recommended name:
            Cathepsin H heavy chain
    3- Recommended name:
            Cathepsin H light chain

Gene names

Name:

Ctsh

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important for the overall degradation of proteins in lysosomes.
Catalytic activity	Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase.
Subunit structure	Composed of a mini chain and a large chain. The large chain may be split into heavy and light chain. All chains are held together by disulfide bonds.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Ref.4 Inferred from expression pattern. Source: RGD
Cellular component	lysosome Ref.4 Traceable author statement. Source: RGD
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro kininogen binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 95	75	Activation peptide		PRO_0000026224
	Chain	96 – 103	8	Cathepsin H mini chain		PRO_0000026225
	Propeptide	104 – 113	10	Potential		PRO_0000026226
	Chain	114 – 333	220	Cathepsin H		PRO_0000026227
	Chain	114 – 290	177	Cathepsin H heavy chain		PRO_0000026228
	Chain	291 – 333	43	Cathepsin H light chain		PRO_0000026229
Sites
	Active site	139	1	By similarity
	Active site	279	1	By similarity
	Active site	299	1	By similarity
Amino acid modifications
	Glycosylation	228	1	N-linked (GlcNAc...) Ref.5
	Disulfide bond	100 ↔ 325		By similarity
	Disulfide bond	136 ↔ 179		By similarity
	Disulfide bond	170 ↔ 212		By similarity
	Disulfide bond	270 ↔ 320		By similarity
Natural variations
	Natural variant	287	1	G → A

Sequences

	Sequence		Length	Mass (Da)
	P00786-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: A90AC66338F0B744	FASTA	333	37,104
	10 20 30 40 50 60 MWTALPLLCA GAWLLSAGAT AELTVNAIEK FHFTSWMKQH QKTYSSREYS HRLQVFANNW 70 80 90 100 110 120 RKIQAHNQRN HTFKMGLNQF SDMSFAEIKH KYLWSEPQNC SATKSNYLRG TGPYPSSMDW 130 140 150 160 170 180 RKKGNVVSPV KNQGACGSCW TFSTTGALES AVAIASGKMM TLAEQQLVDC AQNFNNHGCQ 190 200 210 220 230 240 GGLPSQAFEY ILYNKGIMGE DSYPYIGKNG QCKFNPEKAV AFVKNVVNIT LNDEAAMVEA 250 260 270 280 290 300 VALYNPVSFA FEVTEDFMMY KSGVYSSNSC HKTPDKVNHA VLAVGYGEQN GLLYWIVKNS 310 320 330 WGSNWGNNGY FLIERGKNMC GLAACASYPI PQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequencing of cDNA for rat cathepsin H. Homology in pro-peptide regions of cysteine proteinases." Ishidoh K., Imajoh S., Emori Y., Ohno S., Kawasaki H., Minami Y., Kominami E., Katunuma N., Suzuki K. FEBS Lett. 226:33-37(1987) [PubMed: 3691815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Kidney.
[2]	"Gene structure of rat cathepsin H." Ishidoh K., Kominami E., Katunuma N., Suzuki K. FEBS Lett. 253:103-107(1989) [PubMed: 2759235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"Molecular cloning of rat precursor cathepsin H and the expression of five lysosomal cathepsins in normal tissues and in a rat carcinosarcoma." Qian F., Frankfater A., Miller R.V., Chan S.J., Steiner D.F. Int. J. Biochem. 22:1457-1464(1990) [PubMed: 2276418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-333. Tissue: Liver.
[5]	"Homology of amino acid sequences of rat liver cathepsins B and H with that of papain." Takio K., Towatari T., Katunuma N., Teller D.C., Titani K. Proc. Natl. Acad. Sci. U.S.A. 80:3666-3670(1983) [PubMed: 6574504] [Abstract] Cited for: PROTEIN SEQUENCE OF 114-333. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00708 mRNA. Translation: CAA68699.1. BC085352 mRNA. Translation: AAH85352.1. M38135 mRNA. Translation: AAA63484.1. Different initiation.
IPI	IPI00212809.
PIR	KHRTH. S00211.
RefSeq	NP_037071.1.
UniGene	Rn.1997
3D structure databases
HSSP	HSSP built from PDB template 8PCH based on UniProtKB O46427.
SMR	P00786. Positions 114-333.
ModBase	Search...
Protein family/group databases
MEROPS	C01.040. I29.003.
Proteomic databases
PRIDE	P00786.
Genome annotation databases
Ensembl	ENSRNOG00000014064. Rattus norvegicus. [Contig view]
GeneID	25425.
KEGG	rno:25425.
Organism-specific databases
RGD	2447. Ctsh.
Phylogenomic databases
HOVERGEN	P00786.
OMA	P00786. HHRLQTF.
Enzyme and pathway databases
BRENDA	3.4.22.16. 248.
Gene expression databases
ArrayExpress	P00786.
GermOnline	ENSRNOG00000014064. Rattus norvegicus.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
ProDom	PD000158. Peptidase_C1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	606599.

Entry information

Entry name

CATH_RAT

Accession

Primary (citable) accession number: P00786

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 1, 1988
Last modified:	June 16, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)