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Protein

Papain

Gene
N/A
Organism
Carica papaya (Papaya)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1'.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1581 Publication1
Active sitei2921
Active sitei3081 Publication1

GO - Molecular functioni

  • cysteine-type peptidase activity Source: UniProtKB-KW
  • serpin family protein binding Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.2. 1191.
SABIO-RKP00784.

Protein family/group databases

MEROPSiI29.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Papain (EC:3.4.22.2)
Alternative name(s):
Papaya proteinase I
Short name:
PPI
Allergen: Car p 1
OrganismiCarica papaya (Papaya)
Taxonomic identifieri3649 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesCaricaceaeCarica

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei709. Car p 1.

Chemistry databases

ChEMBLiCHEMBL4779.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000002640619 – 133Activation peptide1 PublicationAdd BLAST115
ChainiPRO_0000026407134 – 345PapainAdd BLAST212

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi155 ↔ 1961 Publication
Disulfide bondi189 ↔ 2281 Publication
Disulfide bondi286 ↔ 3331 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Interactioni

GO - Molecular functioni

  • serpin family protein binding Source: UniProtKB

Protein-protein interaction databases

MINTiMINT-1504623.

Chemistry databases

BindingDBiP00784.

Structurei

Secondary structure

1345
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 38Combined sources4
Helixi41 – 54Combined sources14
Helixi62 – 82Combined sources21
Beta strandi88 – 91Combined sources4
Turni95 – 98Combined sources4
Helixi101 – 106Combined sources6
Beta strandi107 – 109Combined sources3
Beta strandi119 – 124Combined sources6
Turni140 – 144Combined sources5
Beta strandi154 – 156Combined sources3
Helixi158 – 175Combined sources18
Helixi183 – 189Combined sources7
Beta strandi191 – 193Combined sources3
Helixi195 – 197Combined sources3
Helixi201 – 210Combined sources10
Turni216 – 218Combined sources3
Beta strandi223 – 225Combined sources3
Turni230 – 233Combined sources4
Beta strandi241 – 245Combined sources5
Helixi251 – 260Combined sources10
Beta strandi263 – 267Combined sources5
Helixi272 – 275Combined sources4
Beta strandi279 – 282Combined sources4
Beta strandi292 – 300Combined sources9
Beta strandi303 – 307Combined sources5
Beta strandi312 – 314Combined sources3
Turni315 – 317Combined sources3
Beta strandi319 – 323Combined sources5
Beta strandi325 – 327Combined sources3
Helixi332 – 334Combined sources3
Beta strandi340 – 343Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BP4X-ray2.20A134-345[»]
1BQIX-ray2.50A134-345[»]
1CVZX-ray1.70A134-345[»]
1EFFmodel-A134-345[»]
1KHPX-ray2.00A134-345[»]
1KHQX-ray1.60A134-345[»]
1PADX-ray2.80A134-345[»]
1PE6X-ray2.10A134-345[»]
1PIPX-ray1.70A134-345[»]
1POPX-ray2.10A134-345[»]
1PPDX-ray2.00A134-345[»]
1PPNX-ray1.60A134-345[»]
1PPPX-ray1.90A134-345[»]
1STFX-ray2.37E134-345[»]
2CIOX-ray1.50A134-345[»]
2PADX-ray2.80A134-345[»]
3E1ZX-ray1.86B134-345[»]
3IMAX-ray2.03A/C134-345[»]
3LFYX-ray2.60A/C134-345[»]
3TNXX-ray2.62A/C27-345[»]
3USVX-ray3.80A/C27-345[»]
4KP9X-ray2.10A/B134-345[»]
4PADX-ray2.80A134-345[»]
4QRGX-ray2.50A/B27-345[»]
4QRVX-ray1.98A/B27-345[»]
4QRXX-ray3.14A/C27-345[»]
5PADX-ray2.80A134-345[»]
6PADX-ray2.80A134-345[»]
9PAPX-ray1.65A134-345[»]
ProteinModelPortaliP00784.
SMRiP00784.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00784.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00784-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMIPSISKL LFVAICLFVY MGLSFGDFSI VGYSQNDLTS TERLIQLFES
60 70 80 90 100
WMLKHNKIYK NIDEKIYRFE IFKDNLKYID ETNKKNNSYW LGLNVFADMS
110 120 130 140 150
NDEFKEKYTG SIAGNYTTTE LSYEEVLNDG DVNIPEYVDW RQKGAVTPVK
160 170 180 190 200
NQGSCGSCWA FSAVVTIEGI IKIRTGNLNE YSEQELLDCD RRSYGCNGGY
210 220 230 240 250
PWSALQLVAQ YGIHYRNTYP YEGVQRYCRS REKGPYAAKT DGVRQVQPYN
260 270 280 290 300
EGALLYSIAN QPVSVVLEAA GKDFQLYRGG IFVGPCGNKV DHAVAAVGYG
310 320 330 340
PNYILIKNSW GTGWGENGYI RIKRGTGNSY GVCGLYTSSF YPVKN
Length:345
Mass (Da):38,922
Last modified:January 1, 1988 - v1
Checksum:i82D9FB35EDCA12EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180E → Q AA sequence (PubMed:5470818).Curated1
Sequence conflicti219 – 220YP → PY AA sequence (PubMed:5470818).Curated2
Sequence conflicti251E → Q AA sequence (PubMed:5470818).Curated1
Sequence conflicti268E → Q AA sequence (PubMed:5470818).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15203 mRNA. Translation: AAB02650.1.
PIRiA26466. PPPA.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15203 mRNA. Translation: AAB02650.1.
PIRiA26466. PPPA.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BP4X-ray2.20A134-345[»]
1BQIX-ray2.50A134-345[»]
1CVZX-ray1.70A134-345[»]
1EFFmodel-A134-345[»]
1KHPX-ray2.00A134-345[»]
1KHQX-ray1.60A134-345[»]
1PADX-ray2.80A134-345[»]
1PE6X-ray2.10A134-345[»]
1PIPX-ray1.70A134-345[»]
1POPX-ray2.10A134-345[»]
1PPDX-ray2.00A134-345[»]
1PPNX-ray1.60A134-345[»]
1PPPX-ray1.90A134-345[»]
1STFX-ray2.37E134-345[»]
2CIOX-ray1.50A134-345[»]
2PADX-ray2.80A134-345[»]
3E1ZX-ray1.86B134-345[»]
3IMAX-ray2.03A/C134-345[»]
3LFYX-ray2.60A/C134-345[»]
3TNXX-ray2.62A/C27-345[»]
3USVX-ray3.80A/C27-345[»]
4KP9X-ray2.10A/B134-345[»]
4PADX-ray2.80A134-345[»]
4QRGX-ray2.50A/B27-345[»]
4QRVX-ray1.98A/B27-345[»]
4QRXX-ray3.14A/C27-345[»]
5PADX-ray2.80A134-345[»]
6PADX-ray2.80A134-345[»]
9PAPX-ray1.65A134-345[»]
ProteinModelPortaliP00784.
SMRiP00784.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1504623.

Chemistry databases

BindingDBiP00784.
ChEMBLiCHEMBL4779.

Protein family/group databases

Allergomei709. Car p 1.
MEROPSiI29.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.4.22.2. 1191.
SABIO-RKP00784.

Miscellaneous databases

EvolutionaryTraceiP00784.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAPA1_CARPA
AccessioniPrimary (citable) accession number: P00784
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.