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P00777

- PRTB_STRGR

UniProt

P00777 - PRTB_STRGR

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Protein

Streptogrisin-B

Gene

sprB

Organism
Streptomyces griseus
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Has a primary specificity for large aliphatic or aromatic amino acids.

Catalytic activityi

Hydrolysis of proteins with trypsin-like specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei147 – 1471Charge relay system
Active sitei177 – 1771Charge relay system
Active sitei255 – 2551Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.262.

Names & Taxonomyi

Protein namesi
Recommended name:
Streptogrisin-B (EC:3.4.21.81)
Alternative name(s):
Pronase enzyme B
SGPB
Serine protease B
Gene namesi
Name:sprB
OrganismiStreptomyces griseus
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: InterPro
Complete GO annotation...

Pathology & Biotechi

Protein family/group databases

Allergomei3640. Str g Pronase.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3838Add
BLAST
Propeptidei39 – 114761 PublicationPRO_0000026911Add
BLAST
Chaini115 – 299185Streptogrisin-BPRO_0000026912Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi128 ↔ 148
Disulfide bondi249 ↔ 276

Keywords - PTMi

Disulfide bond, Zymogen

Miscellaneous databases

PMAP-CutDBP00777.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

MINTiMINT-1504708.

Structurei

Secondary structure

1
299
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi120 – 1223
Beta strandi127 – 1293
Beta strandi132 – 1365
Beta strandi139 – 1446
Helixi146 – 1494
Beta strandi153 – 1586
Beta strandi163 – 17210
Beta strandi174 – 1763
Beta strandi178 – 1836
Beta strandi186 – 1883
Beta strandi192 – 1943
Beta strandi210 – 2156
Turni216 – 2183
Beta strandi219 – 23315
Beta strandi235 – 2373
Beta strandi239 – 2479
Beta strandi258 – 2614
Beta strandi264 – 27512
Turni276 – 2783
Beta strandi279 – 2868
Helixi287 – 2948

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CSOX-ray1.90E115-299[»]
1CT0X-ray1.80E115-299[»]
1CT2X-ray1.65E115-299[»]
1CT4X-ray1.60E115-299[»]
1DS2X-ray1.70E115-299[»]
1SGDX-ray1.80E115-299[»]
1SGEX-ray1.80E115-299[»]
1SGNX-ray1.80E115-299[»]
1SGPX-ray1.40E115-299[»]
1SGQX-ray1.90E115-299[»]
1SGRX-ray1.80E115-299[»]
1SGYX-ray1.80E115-299[»]
2GKVX-ray1.70E115-260[»]
2NU0X-ray1.95E115-299[»]
2NU1X-ray1.80E115-299[»]
2NU2X-ray1.65E115-299[»]
2NU3X-ray1.80E115-299[»]
2NU4X-ray1.75E115-299[»]
2QA9X-ray1.18E115-299[»]
I119-122[»]
2QAAX-ray1.23A/B115-299[»]
2SGDX-ray1.80E115-299[»]
2SGEX-ray1.80E115-299[»]
2SGFX-ray1.75E115-299[»]
2SGPX-ray1.80E115-299[»]
2SGQX-ray1.80E115-299[»]
3SGBX-ray1.80E115-299[»]
3SGQX-ray1.80E115-299[»]
4SGBX-ray2.10E115-299[»]
ProteinModelPortaliP00777.
SMRiP00777. Positions 115-299.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00777.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S1 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR004236. Pept_S1_alpha_lytic.
IPR001316. Pept_S1A_streptogrisin.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF02983. Pro_Al_protease. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001134. Streptogrisin. 1 hit.
PRINTSiPR00861. ALYTICPTASE.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00777-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRIKRTSNRS NAARRVRTTA VLAGLAAVAA LAVPTANAET PRTFSANQLT
60 70 80 90 100
AASDAVLGAD IAGTAWNIDP QSKRLVVTVD STVSKAEINQ IKKSAGANAD
110 120 130 140 150
ALRIERTPGK FTKLISGGDA IYSSTGRCSL GFNVRSGSTY YFLTAGHCTD
160 170 180 190 200
GATTWWANSA RTTVLGTTSG SSFPNNDYGI VRYTNTTIPK DGTVGGQDIT
210 220 230 240 250
SAANATVGMA VTRRGSTTGT HSGSVTALNA TVNYGGGDVV YGMIRTNVCA
260 270 280 290
EPGDSGGPLY SGTRAIGLTS GGSGNCSSGG TTFFQPVTEA LSAYGVSVY
Length:299
Mass (Da):30,554
Last modified:February 1, 1991 - v2
Checksum:i7A272C9516C7B9B1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti153 – 1531T → TG AA sequence (PubMed:4218101)Curated
Sequence conflicti157 – 1571Missing AA sequence (PubMed:4218101)Curated
Sequence conflicti293 – 2931A → V AA sequence (PubMed:4218101)Curated
Sequence conflicti296 – 2961V → A AA sequence (PubMed:4218101)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17104 Genomic DNA. Translation: AAA26819.1.
PIRiB26974. PRSMBG.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17104 Genomic DNA. Translation: AAA26819.1 .
PIRi B26974. PRSMBG.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CSO X-ray 1.90 E 115-299 [» ]
1CT0 X-ray 1.80 E 115-299 [» ]
1CT2 X-ray 1.65 E 115-299 [» ]
1CT4 X-ray 1.60 E 115-299 [» ]
1DS2 X-ray 1.70 E 115-299 [» ]
1SGD X-ray 1.80 E 115-299 [» ]
1SGE X-ray 1.80 E 115-299 [» ]
1SGN X-ray 1.80 E 115-299 [» ]
1SGP X-ray 1.40 E 115-299 [» ]
1SGQ X-ray 1.90 E 115-299 [» ]
1SGR X-ray 1.80 E 115-299 [» ]
1SGY X-ray 1.80 E 115-299 [» ]
2GKV X-ray 1.70 E 115-260 [» ]
2NU0 X-ray 1.95 E 115-299 [» ]
2NU1 X-ray 1.80 E 115-299 [» ]
2NU2 X-ray 1.65 E 115-299 [» ]
2NU3 X-ray 1.80 E 115-299 [» ]
2NU4 X-ray 1.75 E 115-299 [» ]
2QA9 X-ray 1.18 E 115-299 [» ]
I 119-122 [» ]
2QAA X-ray 1.23 A/B 115-299 [» ]
2SGD X-ray 1.80 E 115-299 [» ]
2SGE X-ray 1.80 E 115-299 [» ]
2SGF X-ray 1.75 E 115-299 [» ]
2SGP X-ray 1.80 E 115-299 [» ]
2SGQ X-ray 1.80 E 115-299 [» ]
3SGB X-ray 1.80 E 115-299 [» ]
3SGQ X-ray 1.80 E 115-299 [» ]
4SGB X-ray 2.10 E 115-299 [» ]
ProteinModelPortali P00777.
SMRi P00777. Positions 115-299.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1504708.

Protein family/group databases

Allergomei 3640. Str g Pronase.
MEROPSi S01.262.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P00777.
PMAP-CutDB P00777.

Family and domain databases

InterProi IPR004236. Pept_S1_alpha_lytic.
IPR001316. Pept_S1A_streptogrisin.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF02983. Pro_Al_protease. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PIRSFi PIRSF001134. Streptogrisin. 1 hit.
PRINTSi PR00861. ALYTICPTASE.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization and structure of genes for proteases A and B from Streptomyces griseus."
    Henderson G., Krygsman P., Liu C.J., Davey C.C., Malek L.T.
    J. Bacteriol. 169:3778-3784(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Amino acid sequence of Streptomyces griseus protease B, A MAJOR COMPONENT OF Pronase."
    Jurasek L., Carpenter M.R., Smillie L.B., Gertler A., Levy S., Ericsson L.H.
    Biochem. Biophys. Res. Commun. 61:1095-1100(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 115-299.
    Strain: K-1.
  3. "Tertiary structural differences between microbial serine proteases and pancreatic serine enzymes."
    Delbaere L.T.J., Hutcheon W.L.B., James M.N.G., Thiessen W.E.
    Nature 257:758-763(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), SEQUENCE REVISION.
  4. "The 2.8-A resolution structure of Streptomyces griseus protease B and its homology with alpha-chymotrypsin and Streptomyces griseus protease A."
    Delbaere L.T.J., Brayer G.D., James M.N.G.
    Can. J. Biochem. 57:135-144(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), SEQUENCE REVISION.
  5. "Structure of the complex of Streptomyces griseus proteinase B and polypeptide chymotrypsin inhibitor-1 from Russet Burbank potato tubers at 2.1-A resolution."
    Greenblatt H.M., Ryan C.A., James M.N.G.
    J. Mol. Biol. 205:201-228(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
  6. "Inhibition of Streptomyces griseus protease B by peptide chloromethyl ketones: partial mapping of the binding site and identification of the reactive residue."
    Gertler A.
    FEBS Lett. 43:81-85(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVE SITE.
  7. "Studies on Streptomyces griseus protease. II. The amino acid sequence around the reactive serine residue of DFP-sensitive components with esterase activity."
    Wahlby S., Engstrom L.
    Biochim. Biophys. Acta 151:402-408(1968) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVE SITE.

Entry informationi

Entry nameiPRTB_STRGR
AccessioniPrimary (citable) accession number: P00777
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1991
Last modified: October 1, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3