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Protein

Streptogrisin-B

Gene

sprB

Organism
Streptomyces griseus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has a primary specificity for large aliphatic or aromatic amino acids.

Catalytic activityi

Hydrolysis of proteins with trypsin-like specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei147Charge relay system1
Active sitei177Charge relay system1
Active sitei255Charge relay system1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.262.

Names & Taxonomyi

Protein namesi
Recommended name:
Streptogrisin-B (EC:3.4.21.81)
Alternative name(s):
Pronase enzyme B
SGPB
Serine protease B
Gene namesi
Name:sprB
OrganismiStreptomyces griseus
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Protein family/group databases

Allergomei3640. Str g Pronase.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 38Add BLAST38
PropeptideiPRO_000002691139 – 1141 PublicationAdd BLAST76
ChainiPRO_0000026912115 – 299Streptogrisin-BAdd BLAST185

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi128 ↔ 148
Disulfide bondi249 ↔ 276

Keywords - PTMi

Disulfide bond, Zymogen

Miscellaneous databases

PMAP-CutDBP00777.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

MINTiMINT-1504708.
STRINGi455632.SGR_5762.

Structurei

Secondary structure

1299
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi120 – 122Combined sources3
Beta strandi127 – 129Combined sources3
Beta strandi132 – 136Combined sources5
Beta strandi139 – 144Combined sources6
Helixi146 – 149Combined sources4
Beta strandi153 – 158Combined sources6
Beta strandi163 – 172Combined sources10
Beta strandi174 – 176Combined sources3
Beta strandi178 – 183Combined sources6
Beta strandi186 – 188Combined sources3
Beta strandi192 – 194Combined sources3
Beta strandi210 – 215Combined sources6
Turni216 – 218Combined sources3
Beta strandi219 – 233Combined sources15
Beta strandi235 – 237Combined sources3
Beta strandi239 – 247Combined sources9
Beta strandi258 – 261Combined sources4
Beta strandi264 – 275Combined sources12
Turni276 – 278Combined sources3
Beta strandi279 – 286Combined sources8
Helixi287 – 294Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CSOX-ray1.90E115-299[»]
1CT0X-ray1.80E115-299[»]
1CT2X-ray1.65E115-299[»]
1CT4X-ray1.60E115-299[»]
1DS2X-ray1.70E115-299[»]
1SGDX-ray1.80E115-299[»]
1SGEX-ray1.80E115-299[»]
1SGNX-ray1.80E115-299[»]
1SGPX-ray1.40E115-299[»]
1SGQX-ray1.90E115-299[»]
1SGRX-ray1.80E115-299[»]
1SGYX-ray1.80E115-299[»]
2GKVX-ray1.70E115-260[»]
2NU0X-ray1.95E115-299[»]
2NU1X-ray1.80E115-299[»]
2NU2X-ray1.65E115-299[»]
2NU3X-ray1.80E115-299[»]
2NU4X-ray1.75E115-299[»]
2QA9X-ray1.18E115-299[»]
2QAAX-ray1.23A/B115-299[»]
2SGDX-ray1.80E115-299[»]
2SGEX-ray1.80E115-299[»]
2SGFX-ray1.75E115-299[»]
2SGPX-ray1.80E115-299[»]
2SGQX-ray1.80E115-299[»]
3SGBX-ray1.80E115-299[»]
3SGQX-ray1.80E115-299[»]
4SGBX-ray2.10E115-299[»]
ProteinModelPortaliP00777.
SMRiP00777.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00777.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4106NMR. Bacteria.
ENOG410YBA6. LUCA.

Family and domain databases

InterProiIPR004236. Pept_S1_alpha_lytic.
IPR001316. Pept_S1A_streptogrisin.
IPR009003. Peptidase_S1_PA.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF02983. Pro_Al_protease. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001134. Streptogrisin. 1 hit.
PRINTSiPR00861. ALYTICPTASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00777-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIKRTSNRS NAARRVRTTA VLAGLAAVAA LAVPTANAET PRTFSANQLT
60 70 80 90 100
AASDAVLGAD IAGTAWNIDP QSKRLVVTVD STVSKAEINQ IKKSAGANAD
110 120 130 140 150
ALRIERTPGK FTKLISGGDA IYSSTGRCSL GFNVRSGSTY YFLTAGHCTD
160 170 180 190 200
GATTWWANSA RTTVLGTTSG SSFPNNDYGI VRYTNTTIPK DGTVGGQDIT
210 220 230 240 250
SAANATVGMA VTRRGSTTGT HSGSVTALNA TVNYGGGDVV YGMIRTNVCA
260 270 280 290
EPGDSGGPLY SGTRAIGLTS GGSGNCSSGG TTFFQPVTEA LSAYGVSVY
Length:299
Mass (Da):30,554
Last modified:February 1, 1991 - v2
Checksum:i7A272C9516C7B9B1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti153T → TG AA sequence (PubMed:4218101).Curated1
Sequence conflicti157Missing AA sequence (PubMed:4218101).Curated1
Sequence conflicti293A → V AA sequence (PubMed:4218101).Curated1
Sequence conflicti296V → A AA sequence (PubMed:4218101).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17104 Genomic DNA. Translation: AAA26819.1.
PIRiB26974. PRSMBG.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17104 Genomic DNA. Translation: AAA26819.1.
PIRiB26974. PRSMBG.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CSOX-ray1.90E115-299[»]
1CT0X-ray1.80E115-299[»]
1CT2X-ray1.65E115-299[»]
1CT4X-ray1.60E115-299[»]
1DS2X-ray1.70E115-299[»]
1SGDX-ray1.80E115-299[»]
1SGEX-ray1.80E115-299[»]
1SGNX-ray1.80E115-299[»]
1SGPX-ray1.40E115-299[»]
1SGQX-ray1.90E115-299[»]
1SGRX-ray1.80E115-299[»]
1SGYX-ray1.80E115-299[»]
2GKVX-ray1.70E115-260[»]
2NU0X-ray1.95E115-299[»]
2NU1X-ray1.80E115-299[»]
2NU2X-ray1.65E115-299[»]
2NU3X-ray1.80E115-299[»]
2NU4X-ray1.75E115-299[»]
2QA9X-ray1.18E115-299[»]
2QAAX-ray1.23A/B115-299[»]
2SGDX-ray1.80E115-299[»]
2SGEX-ray1.80E115-299[»]
2SGFX-ray1.75E115-299[»]
2SGPX-ray1.80E115-299[»]
2SGQX-ray1.80E115-299[»]
3SGBX-ray1.80E115-299[»]
3SGQX-ray1.80E115-299[»]
4SGBX-ray2.10E115-299[»]
ProteinModelPortaliP00777.
SMRiP00777.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1504708.
STRINGi455632.SGR_5762.

Protein family/group databases

Allergomei3640. Str g Pronase.
MEROPSiS01.262.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4106NMR. Bacteria.
ENOG410YBA6. LUCA.

Miscellaneous databases

EvolutionaryTraceiP00777.
PMAP-CutDBP00777.

Family and domain databases

InterProiIPR004236. Pept_S1_alpha_lytic.
IPR001316. Pept_S1A_streptogrisin.
IPR009003. Peptidase_S1_PA.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF02983. Pro_Al_protease. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001134. Streptogrisin. 1 hit.
PRINTSiPR00861. ALYTICPTASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRTB_STRGR
AccessioniPrimary (citable) accession number: P00777
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1991
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.