Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Streptogrisin-A

Gene

sprA

Organism
Streptomyces griseus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has a primary specificity for large aliphatic or aromatic amino acids.

Catalytic activityi

Hydrolysis of proteins with specificity similar to chymotrypsin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei149Charge relay systemBy similarity1
Active sitei171Charge relay systemBy similarity1
Active sitei253Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.261.

Names & Taxonomyi

Protein namesi
Recommended name:
Streptogrisin-A (EC:3.4.21.80)
Alternative name(s):
Pronase enzyme A
SGPA
Serine protease A
Gene namesi
Name:sprA
OrganismiStreptomyces griseus
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Protein family/group databases

Allergomei3640. Str g Pronase.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 38Add BLAST38
PropeptideiPRO_000002690939 – 1151 PublicationAdd BLAST77
ChainiPRO_0000026910116 – 297Streptogrisin-AAdd BLAST182

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi130 ↔ 1501 Publication
Disulfide bondi247 ↔ 2741 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi455632.SGR_370.

Structurei

Secondary structure

1297
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi122 – 125Combined sources4
Beta strandi128 – 131Combined sources4
Beta strandi134 – 138Combined sources5
Beta strandi141 – 146Combined sources6
Helixi148 – 151Combined sources4
Beta strandi155 – 157Combined sources3
Beta strandi160 – 166Combined sources7
Beta strandi168 – 170Combined sources3
Beta strandi172 – 178Combined sources7
Helixi180 – 182Combined sources3
Beta strandi186 – 188Combined sources3
Beta strandi190 – 192Combined sources3
Beta strandi194 – 196Combined sources3
Beta strandi208 – 213Combined sources6
Turni214 – 216Combined sources3
Beta strandi217 – 231Combined sources15
Helixi233 – 235Combined sources3
Beta strandi237 – 245Combined sources9
Beta strandi256 – 259Combined sources4
Beta strandi262 – 273Combined sources12
Turni274 – 276Combined sources3
Beta strandi277 – 284Combined sources8
Helixi285 – 292Combined sources8
Beta strandi294 – 296Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SGCX-ray1.80A117-297[»]
2SGAX-ray1.50A117-297[»]
3SGAX-ray1.80E117-297[»]
4SGAX-ray1.80E117-297[»]
5SGAX-ray1.80E117-297[»]
ProteinModelPortaliP00776.
SMRiP00776.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00776.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S1 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR004236. Pept_S1_alpha_lytic.
IPR001316. Pept_S1A_streptogrisin.
IPR009003. Peptidase_S1_PA.
IPR001254. Trypsin_dom.
[Graphical view]
PfamiPF02983. Pro_Al_protease. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001134. Streptogrisin. 1 hit.
PRINTSiPR00861. ALYTICPTASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFKRFSPLS STSRYARLLA VASGLVAAAA LATPSAVAAP EAESKATVSQ
60 70 80 90 100
LADASSAILA ADVAGTAWYT EASTGKIVLT ADSTVSKAEL AKVSNALAGS
110 120 130 140 150
KAKLTVKRAE GKFTPLIAGG EAITTGGSRC SLGFNVSVNG VAHALTAGHC
160 170 180 190 200
TNISASWSIG TRTGTSFPNN DYGIIRHSNP AAADGRVYLY NGSYQDITTA
210 220 230 240 250
GNAFVGQAVQ RSGSTTGLRS GSVTGLNATV NYGSSGIVYG MIQTNVCAEP
260 270 280 290
GDSGGSLFAG STALGLTSGG SGNCRTGGTT FYQPVTEALS AYGATVL
Length:297
Mass (Da):29,663
Last modified:February 1, 1991 - v2
Checksum:i7F6CF04A81B4B3C9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti151T → TS AA sequence (PubMed:4214713).Curated1
Sequence conflicti184D → N AA sequence (PubMed:4214713).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17103 Genomic DNA. Translation: AAA26818.1.
PIRiA26974. PRSMAG.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17103 Genomic DNA. Translation: AAA26818.1.
PIRiA26974. PRSMAG.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SGCX-ray1.80A117-297[»]
2SGAX-ray1.50A117-297[»]
3SGAX-ray1.80E117-297[»]
4SGAX-ray1.80E117-297[»]
5SGAX-ray1.80E117-297[»]
ProteinModelPortaliP00776.
SMRiP00776.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi455632.SGR_370.

Protein family/group databases

Allergomei3640. Str g Pronase.
MEROPSiS01.261.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP00776.

Family and domain databases

InterProiIPR004236. Pept_S1_alpha_lytic.
IPR001316. Pept_S1A_streptogrisin.
IPR009003. Peptidase_S1_PA.
IPR001254. Trypsin_dom.
[Graphical view]
PfamiPF02983. Pro_Al_protease. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001134. Streptogrisin. 1 hit.
PRINTSiPR00861. ALYTICPTASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRTA_STRGR
AccessioniPrimary (citable) accession number: P00776
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1991
Last modified: November 2, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.