Reviewed, UniProtKB/Swiss-Prot P00776 (PRTA_STRGR)

Names and origin

Protein names

Recommended name:
    Streptogrisin-A
    EC=3.4.21.80
Alternative name(s):
    Serine protease A
    SGPA
    Pronase enzyme A

Gene names

Name:

sprA

Organism

Streptomyces griseus

Taxonomic identifier

1911 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has a primary specificity for large aliphatic or aromatic amino acids.
Catalytic activity	Hydrolysis of proteins with specificity similar to chymotrypsin.
Subunit structure	Monomer.
Sequence similarities	Belongs to the peptidase S1 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 38

38

Propeptide

39 – 115

77

Ref.2

PRO_0000026909

Chain

116 – 297

182

Streptogrisin-A

PRO_0000026910

Sites

Active site

149

1

Charge relay system By similarity

Active site

171

1

Charge relay system By similarity

Active site

253

1

Charge relay system By similarity

Amino acid modifications

Disulfide bond

130 ↔ 150

Ref.5

Disulfide bond

247 ↔ 274

Ref.5

Experimental info

Sequence conflict

151

1

T → TS AA sequence Ref.2

Sequence conflict

184

1

D → N AA sequence Ref.2

Secondary structure

1

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297

Helix Strand Turn

Details...

Beta strand

122 – 125

4

Beta strand

128 – 131

4

Beta strand

134 – 138

5

Beta strand

141 – 146

6

Helix

148 – 151

4

Beta strand

155 – 157

3

Beta strand

160 – 170

11

Beta strand

172 – 178

7

Helix

180 – 182

3

Beta strand

186 – 188

3

Beta strand

190 – 192

3

Beta strand

194 – 196

3

Beta strand

208 – 213

6

Turn

214 – 216

3

Beta strand

217 – 231

15

Helix

233 – 235

3

Beta strand

237 – 245

9

Beta strand

256 – 259

4

Beta strand

262 – 273

12

Turn

274 – 276

3

Beta strand

277 – 284

8

Helix

285 – 292

8

Beta strand

294 – 296

3

Sequences

	Sequence		Length	Mass (Da)
	P00776-1 [UniParc]. Last modified February 1, 1991. Version 2. Checksum: 7F6CF04A81B4B3C9	FASTA	297	29,663
	10 20 30 40 50 60 MTFKRFSPLS STSRYARLLA VASGLVAAAA LATPSAVAAP EAESKATVSQ LADASSAILA 70 80 90 100 110 120 ADVAGTAWYT EASTGKIVLT ADSTVSKAEL AKVSNALAGS KAKLTVKRAE GKFTPLIAGG 130 140 150 160 170 180 EAITTGGSRC SLGFNVSVNG VAHALTAGHC TNISASWSIG TRTGTSFPNN DYGIIRHSNP 190 200 210 220 230 240 AAADGRVYLY NGSYQDITTA GNAFVGQAVQ RSGSTTGLRS GSVTGLNATV NYGSSGIVYG 250 260 270 280 290 MIQTNVCAEP GDSGGSLFAG STALGLTSGG SGNCRTGGTT FYQPVTEALS AYGATVL

References

[1]	"Characterization and structure of genes for proteases A and B from Streptomyces griseus." Henderson G., Krygsman P., Liu C.J., Davey C.C., Malek L.T. J. Bacteriol. 169:3778-3784(1987) [PubMed: 3112129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The amino acid sequence and predicted structure of Streptomyces griseus protease A." Johnson P., Smillie L.B. FEBS Lett. 47:1-6(1974) [PubMed: 4214713] [Abstract] Cited for: PROTEIN SEQUENCE OF 116-297.
[3]	Apostol I., Smillie L.B., Laskowski M. Jr. Submitted (JUL-1996) to UniProtKB Cited for: SEQUENCE REVISION TO 249.
[4]	"Protein structure refinement: Streptomyces griseus serine protease A at 1.8-A resolution." Sielecki A.R., Hendrickson W.A., Broughton C.G., Delbaere L.T.J., Brayer G.D., James M.N.G. J. Mol. Biol. 134:781-804(1979) [PubMed: 119870] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[5]	"Molecular structure of crystalline Streptomyces griseus protease A at 2.8-A resolution. II. Molecular conformation, comparison with alpha-chymotrypsin and active-site geometry." Brayer G.D., Delbaere L.T.J., James M.N.G. J. Mol. Biol. 124:261-283(1978) [PubMed: 101674] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M17103 Genomic DNA. Translation: AAA26818.1.

PIR

PRSMAG. A26974.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SGC	X-ray	1.80	A	117-297	[»]
2SGA	X-ray	1.50	A	117-297	[»]
3SGA	X-ray	1.80	E	117-297	[»]
4SGA	X-ray	1.80	E	117-297	[»]
5SGA	X-ray	1.80	E	117-297	[»]

ModBase

Search...

Protein family/group databases

MEROPS

S01.261.

Enzyme and pathway databases

BRENDA

3.4.21.80. 1270.

Family and domain databases

InterPro

IPR004236. Pept_S1_alpha_lytic.
IPR001316. Pept_S1A_streptogrisin.
IPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
[Graphical view]

Pfam

PF02983. Pro_Al_protease. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]

PIRSF

PIRSF001134. Streptogrisin. 1 hit.

PRINTS

PR00861. ALYTICPTASE.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

PROSITE

PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PRTA_STRGR

Accession

Primary (citable) accession number: P00776

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)