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Protein

Streptogrisin-A

Gene

sprA

Organism
Streptomyces griseus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has a primary specificity for large aliphatic or aromatic amino acids.

Catalytic activityi

Hydrolysis of proteins with specificity similar to chymotrypsin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei149 – 1491Charge relay systemBy similarity
Active sitei171 – 1711Charge relay systemBy similarity
Active sitei253 – 2531Charge relay systemBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.261.

Names & Taxonomyi

Protein namesi
Recommended name:
Streptogrisin-A (EC:3.4.21.80)
Alternative name(s):
Pronase enzyme A
SGPA
Serine protease A
Gene namesi
Name:sprA
OrganismiStreptomyces griseus
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Protein family/group databases

Allergomei3640. Str g Pronase.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3838Add
BLAST
Propeptidei39 – 115771 PublicationPRO_0000026909Add
BLAST
Chaini116 – 297182Streptogrisin-APRO_0000026910Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi130 ↔ 1501 Publication
Disulfide bondi247 ↔ 2741 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi455632.SGR_370.

Structurei

Secondary structure

1
297
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi122 – 1254Combined sources
Beta strandi128 – 1314Combined sources
Beta strandi134 – 1385Combined sources
Beta strandi141 – 1466Combined sources
Helixi148 – 1514Combined sources
Beta strandi155 – 1573Combined sources
Beta strandi160 – 1667Combined sources
Beta strandi168 – 1703Combined sources
Beta strandi172 – 1787Combined sources
Helixi180 – 1823Combined sources
Beta strandi186 – 1883Combined sources
Beta strandi190 – 1923Combined sources
Beta strandi194 – 1963Combined sources
Beta strandi208 – 2136Combined sources
Turni214 – 2163Combined sources
Beta strandi217 – 23115Combined sources
Helixi233 – 2353Combined sources
Beta strandi237 – 2459Combined sources
Beta strandi256 – 2594Combined sources
Beta strandi262 – 27312Combined sources
Turni274 – 2763Combined sources
Beta strandi277 – 2848Combined sources
Helixi285 – 2928Combined sources
Beta strandi294 – 2963Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SGCX-ray1.80A117-297[»]
2SGAX-ray1.50A117-297[»]
3SGAX-ray1.80E117-297[»]
4SGAX-ray1.80E117-297[»]
5SGAX-ray1.80E117-297[»]
ProteinModelPortaliP00776.
SMRiP00776. Positions 117-297.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00776.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S1 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR004236. Pept_S1_alpha_lytic.
IPR001316. Pept_S1A_streptogrisin.
IPR009003. Peptidase_S1_PA.
IPR001254. Trypsin_dom.
[Graphical view]
PfamiPF02983. Pro_Al_protease. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001134. Streptogrisin. 1 hit.
PRINTSiPR00861. ALYTICPTASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFKRFSPLS STSRYARLLA VASGLVAAAA LATPSAVAAP EAESKATVSQ
60 70 80 90 100
LADASSAILA ADVAGTAWYT EASTGKIVLT ADSTVSKAEL AKVSNALAGS
110 120 130 140 150
KAKLTVKRAE GKFTPLIAGG EAITTGGSRC SLGFNVSVNG VAHALTAGHC
160 170 180 190 200
TNISASWSIG TRTGTSFPNN DYGIIRHSNP AAADGRVYLY NGSYQDITTA
210 220 230 240 250
GNAFVGQAVQ RSGSTTGLRS GSVTGLNATV NYGSSGIVYG MIQTNVCAEP
260 270 280 290
GDSGGSLFAG STALGLTSGG SGNCRTGGTT FYQPVTEALS AYGATVL
Length:297
Mass (Da):29,663
Last modified:February 1, 1991 - v2
Checksum:i7F6CF04A81B4B3C9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti151 – 1511T → TS AA sequence (PubMed:4214713).Curated
Sequence conflicti184 – 1841D → N AA sequence (PubMed:4214713).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17103 Genomic DNA. Translation: AAA26818.1.
PIRiA26974. PRSMAG.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17103 Genomic DNA. Translation: AAA26818.1.
PIRiA26974. PRSMAG.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SGCX-ray1.80A117-297[»]
2SGAX-ray1.50A117-297[»]
3SGAX-ray1.80E117-297[»]
4SGAX-ray1.80E117-297[»]
5SGAX-ray1.80E117-297[»]
ProteinModelPortaliP00776.
SMRiP00776. Positions 117-297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi455632.SGR_370.

Protein family/group databases

Allergomei3640. Str g Pronase.
MEROPSiS01.261.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP00776.

Family and domain databases

InterProiIPR004236. Pept_S1_alpha_lytic.
IPR001316. Pept_S1A_streptogrisin.
IPR009003. Peptidase_S1_PA.
IPR001254. Trypsin_dom.
[Graphical view]
PfamiPF02983. Pro_Al_protease. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001134. Streptogrisin. 1 hit.
PRINTSiPR00861. ALYTICPTASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization and structure of genes for proteases A and B from Streptomyces griseus."
    Henderson G., Krygsman P., Liu C.J., Davey C.C., Malek L.T.
    J. Bacteriol. 169:3778-3784(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The amino acid sequence and predicted structure of Streptomyces griseus protease A."
    Johnson P., Smillie L.B.
    FEBS Lett. 47:1-6(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 116-297.
  3. Apostol I., Smillie L.B., Laskowski M. Jr.
    Submitted (JUL-1996) to UniProtKB
    Cited for: SEQUENCE REVISION TO 249.
  4. "Protein structure refinement: Streptomyces griseus serine protease A at 1.8-A resolution."
    Sielecki A.R., Hendrickson W.A., Broughton C.G., Delbaere L.T.J., Brayer G.D., James M.N.G.
    J. Mol. Biol. 134:781-804(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
  5. "Molecular structure of crystalline Streptomyces griseus protease A at 2.8-A resolution. II. Molecular conformation, comparison with alpha-chymotrypsin and active-site geometry."
    Brayer G.D., Delbaere L.T.J., James M.N.G.
    J. Mol. Biol. 124:261-283(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

Entry informationi

Entry nameiPRTA_STRGR
AccessioniPrimary (citable) accession number: P00776
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1991
Last modified: May 11, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.