Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Trypsin

Gene

sprT

Organism
Streptomyces griseus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73Charge relay system1
Active sitei118Charge relay system1
Sitei202Required for specificity1
Active sitei208Charge relay system1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.4. 6035.

Protein family/group databases

MEROPSiS01.101.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin (EC:3.4.21.4)
Alternative name(s):
SGT
Gene namesi
Name:sprT
OrganismiStreptomyces griseus
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Add BLAST32
PropeptideiPRO_000002830733 – 36Activation peptide1 Publication4
ChainiPRO_000002830837 – 259TrypsinAdd BLAST223

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi58 ↔ 74
Disulfide bondi177 ↔ 192
Disulfide bondi204 ↔ 233

Keywords - PTMi

Disulfide bond, Zymogen

Interactioni

Protein-protein interaction databases

STRINGi455632.SGR_1241.

Structurei

Secondary structure

1259
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni45 – 47Combined sources3
Beta strandi51 – 54Combined sources4
Turni55 – 57Combined sources3
Beta strandi58 – 64Combined sources7
Beta strandi67 – 70Combined sources4
Helixi72 – 74Combined sources3
Beta strandi77 – 81Combined sources5
Beta strandi85 – 89Combined sources5
Beta strandi91 – 95Combined sources5
Beta strandi99 – 108Combined sources10
Beta strandi113 – 116Combined sources4
Beta strandi120 – 126Combined sources7
Beta strandi137 – 139Combined sources3
Beta strandi142 – 152Combined sources11
Beta strandi165 – 172Combined sources8
Helixi174 – 180Combined sources7
Helixi182 – 184Combined sources3
Helixi187 – 189Combined sources3
Beta strandi190 – 193Combined sources4
Beta strandi196 – 199Combined sources4
Beta strandi211 – 215Combined sources5
Beta strandi221 – 234Combined sources14
Beta strandi240 – 244Combined sources5
Helixi245 – 256Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OS8X-ray1.55A37-252[»]
1OSSX-ray1.93A37-252[»]
1SGTX-ray1.70A37-259[»]
2FMJX-ray1.65A37-251[»]
3BEUX-ray1.05A/B37-259[»]
3I77X-ray2.10A37-259[»]
3I78X-ray3.00A37-259[»]
ProteinModelPortaliP00775.
SMRiP00775.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00775.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 257Peptidase S1PROSITE-ProRule annotationAdd BLAST221

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105NDH. Bacteria.
COG5640. LUCA.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00775-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHFLRALKR CSVAVATVAI AVVGLQPVTA SAAPNPVVGG TRAAQGEFPF
60 70 80 90 100
MVRLSMGCGG ALYAQDIVLT AAHCVSGSGN NTSITATGGV VDLQSSSAVK
110 120 130 140 150
VRSTKVLQAP GYNGTGKDWA LIKLAQPINQ PTLKIATTTA YNQGTFTVAG
160 170 180 190 200
WGANREGGSQ QRYLLKANVP FVSDAACRSA YGNELVANEE ICAGYPDTGG
210 220 230 240 250
VDTCQGDSGG PMFRKDNADE WIQVGIVSWG YGCARPGYPG VYTEVSTFAS

AIASAARTL
Length:259
Mass (Da):26,776
Last modified:February 1, 1994 - v2
Checksum:i050233AFF1F64823
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95 – 96Missing AA sequence (PubMed:804314).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64471 Genomic DNA. Translation: AAA26820.1. Sequence problems.
PIRiJQ1302. TRSMG.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64471 Genomic DNA. Translation: AAA26820.1. Sequence problems.
PIRiJQ1302. TRSMG.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OS8X-ray1.55A37-252[»]
1OSSX-ray1.93A37-252[»]
1SGTX-ray1.70A37-259[»]
2FMJX-ray1.65A37-251[»]
3BEUX-ray1.05A/B37-259[»]
3I77X-ray2.10A37-259[»]
3I78X-ray3.00A37-259[»]
ProteinModelPortaliP00775.
SMRiP00775.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi455632.SGR_1241.

Protein family/group databases

MEROPSiS01.101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105NDH. Bacteria.
COG5640. LUCA.

Enzyme and pathway databases

BRENDAi3.4.21.4. 6035.

Miscellaneous databases

EvolutionaryTraceiP00775.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRYP_STRGR
AccessioniPrimary (citable) accession number: P00775
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.