Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P00775

- TRYP_STRGR

UniProt

P00775 - TRYP_STRGR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Trypsin

Gene

sprT

Organism
Streptomyces griseus
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731Charge relay system
Active sitei118 – 1181Charge relay system
Sitei202 – 2021Required for specificity
Active sitei208 – 2081Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.4. 6035.

Protein family/group databases

MEROPSiS01.101.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin (EC:3.4.21.4)
Alternative name(s):
SGT
Gene namesi
Name:sprT
OrganismiStreptomyces griseus
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Add
BLAST
Propeptidei33 – 364Activation peptide1 PublicationPRO_0000028307
Chaini37 – 259223TrypsinPRO_0000028308Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi58 ↔ 74
Disulfide bondi177 ↔ 192
Disulfide bondi204 ↔ 233

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

Secondary structure

1
259
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni45 – 473Combined sources
Beta strandi51 – 544Combined sources
Turni55 – 573Combined sources
Beta strandi58 – 647Combined sources
Beta strandi67 – 704Combined sources
Helixi72 – 743Combined sources
Beta strandi77 – 815Combined sources
Beta strandi85 – 895Combined sources
Beta strandi91 – 955Combined sources
Beta strandi99 – 10810Combined sources
Beta strandi113 – 1164Combined sources
Beta strandi120 – 1267Combined sources
Beta strandi137 – 1393Combined sources
Beta strandi142 – 15211Combined sources
Beta strandi165 – 1728Combined sources
Helixi174 – 1807Combined sources
Helixi182 – 1843Combined sources
Helixi187 – 1893Combined sources
Beta strandi190 – 1934Combined sources
Beta strandi196 – 1994Combined sources
Beta strandi211 – 2155Combined sources
Beta strandi221 – 23414Combined sources
Beta strandi240 – 2445Combined sources
Helixi245 – 25612Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OS8X-ray1.55A37-252[»]
1OSSX-ray1.93A37-252[»]
1SGTX-ray1.70A37-259[»]
2FMJX-ray1.65A37-251[»]
3BEUX-ray1.05A/B37-259[»]
3I77X-ray2.10A37-259[»]
3I78X-ray3.00A37-259[»]
ProteinModelPortaliP00775.
SMRiP00775. Positions 37-259.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00775.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 257221Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00775-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKHFLRALKR CSVAVATVAI AVVGLQPVTA SAAPNPVVGG TRAAQGEFPF
60 70 80 90 100
MVRLSMGCGG ALYAQDIVLT AAHCVSGSGN NTSITATGGV VDLQSSSAVK
110 120 130 140 150
VRSTKVLQAP GYNGTGKDWA LIKLAQPINQ PTLKIATTTA YNQGTFTVAG
160 170 180 190 200
WGANREGGSQ QRYLLKANVP FVSDAACRSA YGNELVANEE ICAGYPDTGG
210 220 230 240 250
VDTCQGDSGG PMFRKDNADE WIQVGIVSWG YGCARPGYPG VYTEVSTFAS

AIASAARTL
Length:259
Mass (Da):26,776
Last modified:February 1, 1994 - v2
Checksum:i050233AFF1F64823
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 962Missing AA sequence (PubMed:804314)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64471 Genomic DNA. Translation: AAA26820.1. Sequence problems.
PIRiJQ1302. TRSMG.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64471 Genomic DNA. Translation: AAA26820.1 . Sequence problems.
PIRi JQ1302. TRSMG.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1OS8 X-ray 1.55 A 37-252 [» ]
1OSS X-ray 1.93 A 37-252 [» ]
1SGT X-ray 1.70 A 37-259 [» ]
2FMJ X-ray 1.65 A 37-251 [» ]
3BEU X-ray 1.05 A/B 37-259 [» ]
3I77 X-ray 2.10 A 37-259 [» ]
3I78 X-ray 3.00 A 37-259 [» ]
ProteinModelPortali P00775.
SMRi P00775. Positions 37-259.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.101.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 3.4.21.4. 6035.

Miscellaneous databases

EvolutionaryTracei P00775.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and nucleotide sequence of Streptomyces griseus trypsin gene."
    Kim J.C., Cha S.H., Jeong S.T., Oh S.K., Byun S.M.
    Biochem. Biophys. Res. Commun. 181:707-713(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10137 / DSM 40855 / NBRC 3430 / NCIMB 8232 / NCTC 6961.
  2. "Amino acid sequence of Streptomyces griseus trypsin. Cyanogen bromide fragments and complete sequence."
    Olafson R.W., Jurasek L., Carpenter M.R., Smillie L.B.
    Biochemistry 14:1168-1177(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 37-259.
  3. "Refined crystal structure of Streptomyces griseus trypsin at 1.7-A resolution."
    Read R.J., James M.N.G.
    J. Mol. Biol. 200:523-551(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

Entry informationi

Entry nameiTRYP_STRGR
AccessioniPrimary (citable) accession number: P00775
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1994
Last modified: November 26, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3