P00774 (CEL2A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chymotrypsin-like elastase family member 2A
EC=3.4.21.71

Alternative name(s):
Elastase-2
Elastase-2A

Gene names

Name:	Cela2a
Synonyms:	Ela2, Ela2a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts upon elastin.
Catalytic activity	Preferential cleavage: Leu-|-Xaa, Met-|-Xaa and Phe-|-Xaa. Hydrolyzes elastin.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16
	Propeptide	17 – 30	14	Activation peptide		PRO_0000027691
	Chain	31 – 271	241	Chymotrypsin-like elastase family member 2A		PRO_0000027692
Regions
	Domain	31 – 269	239	Peptidase S1
Sites
	Active site	75	1	Charge relay system By similarity
	Active site	123	1	Charge relay system By similarity
	Active site	218	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	60 ↔ 76		By similarity
	Disulfide bond	157 ↔ 224		By similarity
	Disulfide bond	188 ↔ 204		By similarity
	Disulfide bond	214 ↔ 245		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00774 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 125C783B857B71E3	FASTA	271	28,885
	10 20 30 40 50 60 MIRTLLLSAL VAGALSCGYP TYEVQHDVSR VVGGQEASPN SWPWQVSLQY LSSGKWHHTC 70 80 90 100 110 120 GGSLVANNWV LTAAHCISNS RTYRVLLGRH SLSTSESGSL AVQVSKLVVH EKWNAQKLSN 130 140 150 160 170 180 GNDIALVKLA SPVALTSKIQ TACLPPAGTI LPNNYPCYVT GWGRLQTNGA TPDVLQQGRL 190 200 210 220 230 240 LVVDYATCSS ASWWGSSVKT NMVCAGGDGV TSSCNGDSGG PLNCQASNGQ WQVHGIVSFG 250 260 270 STLGCNYPRK PSVFTRVSNY IDWINSVIAK N

References

[1]	"Primary structure of two distinct rat pancreatic preproelastases determined by sequence analysis of the complete cloned messenger ribonucleic acid sequences." MacDonald R.J., Swift G.H., Quinto C., Swain W., Pictet R.L., Nikovits W., Rutter W.J. Biochemistry 21:1453-1463(1982) [PubMed: 6918221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure of the two related elastase genes expressed in the rat pancreas." Swift G.H., Craik C.S., Stary S.J., Quinto C., Lahaie R.G., Rutter W.J., MacDonald R.J. J. Biol. Chem. 259:14271-14278(1984) [PubMed: 6094548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V01233 mRNA. Translation: CAA24543.1. L00124 , L00118, L00119, L00120, L00121, L00122, L00123 Genomic DNA. Translation: AAA98780.1.
IPI	IPI00212792.
PIR	ELRT2. A00961.
RefSeq	NP_036685.1. NM_012553.1.
UniGene	Rn.10272.
3D structure databases
ProteinModelPortal	P00774.
SMR	P00774. Positions 31-271.
ModBase	Search...
Protein-protein interaction databases
STRING	P00774.
Protein family/group databases
MEROPS	S01.155.
Proteomic databases
PRIDE	P00774.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000018349; ENSRNOP00000018349; ENSRNOG00000013628.
GeneID	24332.
KEGG	rno:24332.
UCSC	NM_012553. rat.
Organism-specific databases
CTD	63036.
RGD	2548. Cela2a.
Phylogenomic databases
eggNOG	maNOG05852.
GeneTree	ENSGT00550000074117.
InParanoid	P00774.
OrthoDB	EOG4XPQGN.
PhylomeDB	P00774.
Gene expression databases
ArrayExpress	P00774.
Genevestigator	P00774.
GermOnline	ENSRNOG00000013628. Rattus norvegicus.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
KO	K01346.
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603011.

Entry information

Entry name

CEL2A_RAT

Accession

Primary (citable) accession number: P00774

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 16, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program