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Protein

Chymotrypsin-like elastase family member 2A

Gene

Cela2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Acts upon elastin.

Catalytic activityi

Preferential cleavage: Leu-|-Xaa, Met-|-Xaa and Phe-|-Xaa. Hydrolyzes elastin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751Charge relay systemBy similarity
Active sitei123 – 1231Charge relay systemBy similarity
Active sitei218 – 2181Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. establishment of skin barrier Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.155.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsin-like elastase family member 2A (EC:3.4.21.71)
Alternative name(s):
Elastase-2
Elastase-2A
Gene namesi
Name:Cela2a
Synonyms:Ela2, Ela2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2548. Cela2a.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Add
BLAST
Propeptidei17 – 3014Activation peptidePRO_0000027691Add
BLAST
Chaini31 – 271241Chymotrypsin-like elastase family member 2APRO_0000027692Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi60 ↔ 76PROSITE-ProRule annotation
Disulfide bondi157 ↔ 224PROSITE-ProRule annotation
Disulfide bondi188 ↔ 204PROSITE-ProRule annotation
Disulfide bondi214 ↔ 245PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP00774.
PRIDEiP00774.

PTM databases

PhosphoSiteiP00774.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

GenevestigatoriP00774.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018349.

Structurei

3D structure databases

ProteinModelPortaliP00774.
SMRiP00774. Positions 31-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 269239Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
InParanoidiP00774.
KOiK01346.
OMAiASNGQWQ.
OrthoDBiEOG75B84T.
PhylomeDBiP00774.
TreeFamiTF330455.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRTLLLSAL VAGALSCGYP TYEVQHDVSR VVGGQEASPN SWPWQVSLQY
60 70 80 90 100
LSSGKWHHTC GGSLVANNWV LTAAHCISNS RTYRVLLGRH SLSTSESGSL
110 120 130 140 150
AVQVSKLVVH EKWNAQKLSN GNDIALVKLA SPVALTSKIQ TACLPPAGTI
160 170 180 190 200
LPNNYPCYVT GWGRLQTNGA TPDVLQQGRL LVVDYATCSS ASWWGSSVKT
210 220 230 240 250
NMVCAGGDGV TSSCNGDSGG PLNCQASNGQ WQVHGIVSFG STLGCNYPRK
260 270
PSVFTRVSNY IDWINSVIAK N
Length:271
Mass (Da):28,885
Last modified:July 21, 1986 - v1
Checksum:i125C783B857B71E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01233 mRNA. Translation: CAA24543.1.
L00124
, L00118, L00119, L00120, L00121, L00122, L00123 Genomic DNA. Translation: AAA98780.1.
PIRiA00961. ELRT2.
RefSeqiNP_036685.1. NM_012553.2.
UniGeneiRn.10272.

Genome annotation databases

EnsembliENSRNOT00000018349; ENSRNOP00000018349; ENSRNOG00000013628.
GeneIDi24332.
KEGGirno:24332.
UCSCiRGD:2548. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01233 mRNA. Translation: CAA24543.1.
L00124
, L00118, L00119, L00120, L00121, L00122, L00123 Genomic DNA. Translation: AAA98780.1.
PIRiA00961. ELRT2.
RefSeqiNP_036685.1. NM_012553.2.
UniGeneiRn.10272.

3D structure databases

ProteinModelPortaliP00774.
SMRiP00774. Positions 31-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018349.

Chemistry

BindingDBiP00774.
ChEMBLiCHEMBL3888.

Protein family/group databases

MEROPSiS01.155.

PTM databases

PhosphoSiteiP00774.

Proteomic databases

PaxDbiP00774.
PRIDEiP00774.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018349; ENSRNOP00000018349; ENSRNOG00000013628.
GeneIDi24332.
KEGGirno:24332.
UCSCiRGD:2548. rat.

Organism-specific databases

CTDi63036.
RGDi2548. Cela2a.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
InParanoidiP00774.
KOiK01346.
OMAiASNGQWQ.
OrthoDBiEOG75B84T.
PhylomeDBiP00774.
TreeFamiTF330455.

Miscellaneous databases

NextBioi603011.
PROiP00774.

Gene expression databases

GenevestigatoriP00774.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure of two distinct rat pancreatic preproelastases determined by sequence analysis of the complete cloned messenger ribonucleic acid sequences."
    MacDonald R.J., Swift G.H., Quinto C., Swain W., Pictet R.L., Nikovits W., Rutter W.J.
    Biochemistry 21:1453-1463(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structure of the two related elastase genes expressed in the rat pancreas."
    Swift G.H., Craik C.S., Stary S.J., Quinto C., Lahaie R.G., Rutter W.J., MacDonald R.J.
    J. Biol. Chem. 259:14271-14278(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiCEL2A_RAT
AccessioniPrimary (citable) accession number: P00774
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: January 7, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.