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P00774

- CEL2A_RAT

UniProt

P00774 - CEL2A_RAT

Protein

Chymotrypsin-like elastase family member 2A

Gene

Cela2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Acts upon elastin.

    Catalytic activityi

    Preferential cleavage: Leu-|-Xaa, Met-|-Xaa and Phe-|-Xaa. Hydrolyzes elastin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei75 – 751Charge relay systemBy similarity
    Active sitei123 – 1231Charge relay systemBy similarity
    Active sitei218 – 2181Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.155.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chymotrypsin-like elastase family member 2A (EC:3.4.21.71)
    Alternative name(s):
    Elastase-2
    Elastase-2A
    Gene namesi
    Name:Cela2a
    Synonyms:Ela2, Ela2a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi2548. Cela2a.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Add
    BLAST
    Propeptidei17 – 3014Activation peptidePRO_0000027691Add
    BLAST
    Chaini31 – 271241Chymotrypsin-like elastase family member 2APRO_0000027692Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi60 ↔ 76PROSITE-ProRule annotation
    Disulfide bondi157 ↔ 224PROSITE-ProRule annotation
    Disulfide bondi188 ↔ 204PROSITE-ProRule annotation
    Disulfide bondi214 ↔ 245PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Proteomic databases

    PaxDbiP00774.
    PRIDEiP00774.

    PTM databases

    PhosphoSiteiP00774.

    Expressioni

    Tissue specificityi

    Pancreas.

    Gene expression databases

    GenevestigatoriP00774.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000018349.

    Structurei

    3D structure databases

    ProteinModelPortaliP00774.
    SMRiP00774. Positions 31-271.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 269239Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00640000091262.
    HOGENOMiHOG000251820.
    InParanoidiP00774.
    KOiK01346.
    OMAiASNGQWQ.
    OrthoDBiEOG75B84T.
    PhylomeDBiP00774.
    TreeFamiTF330455.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00774-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIRTLLLSAL VAGALSCGYP TYEVQHDVSR VVGGQEASPN SWPWQVSLQY    50
    LSSGKWHHTC GGSLVANNWV LTAAHCISNS RTYRVLLGRH SLSTSESGSL 100
    AVQVSKLVVH EKWNAQKLSN GNDIALVKLA SPVALTSKIQ TACLPPAGTI 150
    LPNNYPCYVT GWGRLQTNGA TPDVLQQGRL LVVDYATCSS ASWWGSSVKT 200
    NMVCAGGDGV TSSCNGDSGG PLNCQASNGQ WQVHGIVSFG STLGCNYPRK 250
    PSVFTRVSNY IDWINSVIAK N 271
    Length:271
    Mass (Da):28,885
    Last modified:July 21, 1986 - v1
    Checksum:i125C783B857B71E3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01233 mRNA. Translation: CAA24543.1.
    L00124
    , L00118, L00119, L00120, L00121, L00122, L00123 Genomic DNA. Translation: AAA98780.1.
    PIRiA00961. ELRT2.
    RefSeqiNP_036685.1. NM_012553.2.
    UniGeneiRn.10272.

    Genome annotation databases

    EnsembliENSRNOT00000018349; ENSRNOP00000018349; ENSRNOG00000013628.
    GeneIDi24332.
    KEGGirno:24332.
    UCSCiRGD:2548. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01233 mRNA. Translation: CAA24543.1 .
    L00124
    , L00118 , L00119 , L00120 , L00121 , L00122 , L00123 Genomic DNA. Translation: AAA98780.1 .
    PIRi A00961. ELRT2.
    RefSeqi NP_036685.1. NM_012553.2.
    UniGenei Rn.10272.

    3D structure databases

    ProteinModelPortali P00774.
    SMRi P00774. Positions 31-271.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000018349.

    Chemistry

    BindingDBi P00774.
    ChEMBLi CHEMBL3888.

    Protein family/group databases

    MEROPSi S01.155.

    PTM databases

    PhosphoSitei P00774.

    Proteomic databases

    PaxDbi P00774.
    PRIDEi P00774.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000018349 ; ENSRNOP00000018349 ; ENSRNOG00000013628 .
    GeneIDi 24332.
    KEGGi rno:24332.
    UCSCi RGD:2548. rat.

    Organism-specific databases

    CTDi 63036.
    RGDi 2548. Cela2a.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00640000091262.
    HOGENOMi HOG000251820.
    InParanoidi P00774.
    KOi K01346.
    OMAi ASNGQWQ.
    OrthoDBi EOG75B84T.
    PhylomeDBi P00774.
    TreeFami TF330455.

    Miscellaneous databases

    NextBioi 603011.
    PROi P00774.

    Gene expression databases

    Genevestigatori P00774.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of two distinct rat pancreatic preproelastases determined by sequence analysis of the complete cloned messenger ribonucleic acid sequences."
      MacDonald R.J., Swift G.H., Quinto C., Swain W., Pictet R.L., Nikovits W., Rutter W.J.
      Biochemistry 21:1453-1463(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structure of the two related elastase genes expressed in the rat pancreas."
      Swift G.H., Craik C.S., Stary S.J., Quinto C., Lahaie R.G., Rutter W.J., MacDonald R.J.
      J. Biol. Chem. 259:14271-14278(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiCEL2A_RAT
    AccessioniPrimary (citable) accession number: P00774
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3