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Protein

Chymotrypsin-like elastase family member 1

Gene

Cela1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts upon elastin.

Catalytic activityi

Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei71Charge relay systemBy similarity1
Metal bindingi85CalciumBy similarity1
Metal bindingi90Calcium; via carbonyl oxygenBy similarity1
Metal bindingi95CalciumBy similarity1
Active sitei119Charge relay systemBy similarity1
Active sitei214Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS01.153.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsin-like elastase family member 1 (EC:3.4.21.36)
Alternative name(s):
Elastase-1
Gene namesi
Name:Cela1
Synonyms:Ela1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2547. Cela1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 161 PublicationAdd BLAST16
PropeptideiPRO_000002768317 – 26Activation peptide10
ChainiPRO_000002768427 – 266Chymotrypsin-like elastase family member 1Add BLAST240

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi56 ↔ 72PROSITE-ProRule annotation
Disulfide bondi153 ↔ 220PROSITE-ProRule annotation
Disulfide bondi184 ↔ 200PROSITE-ProRule annotation
Disulfide bondi210 ↔ 240PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP00773.
PRIDEiP00773.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

BgeeiENSRNOG00000004725.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006351.

Structurei

3D structure databases

ProteinModelPortaliP00773.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 264Peptidase S1PROSITE-ProRule annotationAdd BLAST238

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
InParanoidiP00773.
KOiK01326.
PhylomeDBiP00773.
TreeFamiTF330455.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRFLVFASL VLYGHSTQDF PETNARVVGG AEARRNSWPS QISLQYLSGG
60 70 80 90 100
SWYHTCGGTL IRRNWVMTAA HCVSSQMTFR VVVGDHNLSQ NDGTEQYVSV
110 120 130 140 150
QKIMVHPTWN SNNVAAGYDI ALLRLAQSVT LNNYVQLAVL PQEGTILANN
160 170 180 190 200
NPCYITGWGR TRTNGQLSQT LQQAYLPSVD YSICSSSSYW GSTVKTTMVC
210 220 230 240 250
AGGDGVRSGC QGDSGGPLHC LVNGQYSVHG VTSFVSSMGC NVSKKPTVFT
260
RVSAYISWMN NVIAYT
Length:266
Mass (Da):28,976
Last modified:July 21, 1986 - v1
Checksum:i5A56FE8FCF1AAEDA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104M → V in AAA98811 (PubMed:6094548).Curated1
Sequence conflicti108T → N in AAA98811 (PubMed:6094548).Curated1
Sequence conflicti244K → R in AAA98811 (PubMed:6094548).Curated1
Sequence conflicti266T → N in AAA98811 (PubMed:6094548).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01234 mRNA. Translation: CAA24544.1.
L00117
, L00112, L00113, L00114, L00115, L00116 Genomic DNA. Translation: AAA98811.1.
PIRiA00960. ELRT1.
RefSeqiNP_036684.1. NM_012552.4.
UniGeneiRn.6044.

Genome annotation databases

GeneIDi24331.
KEGGirno:24331.
UCSCiRGD:2547. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01234 mRNA. Translation: CAA24544.1.
L00117
, L00112, L00113, L00114, L00115, L00116 Genomic DNA. Translation: AAA98811.1.
PIRiA00960. ELRT1.
RefSeqiNP_036684.1. NM_012552.4.
UniGeneiRn.6044.

3D structure databases

ProteinModelPortaliP00773.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006351.

Protein family/group databases

MEROPSiS01.153.

Proteomic databases

PaxDbiP00773.
PRIDEiP00773.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24331.
KEGGirno:24331.
UCSCiRGD:2547. rat.

Organism-specific databases

CTDi1990.
RGDi2547. Cela1.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
InParanoidiP00773.
KOiK01326.
PhylomeDBiP00773.
TreeFamiTF330455.

Miscellaneous databases

PROiP00773.

Gene expression databases

BgeeiENSRNOG00000004725.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCELA1_RAT
AccessioniPrimary (citable) accession number: P00773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 5, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.