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P00773

- CELA1_RAT

UniProt

P00773 - CELA1_RAT

Protein

Chymotrypsin-like elastase family member 1

Gene

Cela1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Acts upon elastin.

    Catalytic activityi

    Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei71 – 711Charge relay systemBy similarity
    Metal bindingi85 – 851CalciumBy similarity
    Metal bindingi90 – 901Calcium; via carbonyl oxygenBy similarity
    Metal bindingi95 – 951CalciumBy similarity
    Active sitei119 – 1191Charge relay systemBy similarity
    Active sitei214 – 2141Charge relay systemBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. exocrine pancreas development Source: Ensembl
    2. inflammatory response Source: Ensembl
    3. multicellular organism growth Source: Ensembl
    4. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    5. pancreas morphogenesis Source: Ensembl
    6. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    7. post-embryonic development Source: Ensembl
    8. regulation of cell differentiation Source: Ensembl
    9. regulation of cell proliferation Source: Ensembl
    10. Wnt signaling pathway Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    MEROPSiS01.153.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chymotrypsin-like elastase family member 1 (EC:3.4.21.36)
    Alternative name(s):
    Elastase-1
    Gene namesi
    Name:Cela1
    Synonyms:Ela1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi2547. Cela1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 16161 PublicationAdd
    BLAST
    Propeptidei17 – 2610Activation peptidePRO_0000027683
    Chaini27 – 266240Chymotrypsin-like elastase family member 1PRO_0000027684Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi56 ↔ 72PROSITE-ProRule annotation
    Disulfide bondi153 ↔ 220PROSITE-ProRule annotation
    Disulfide bondi184 ↔ 200PROSITE-ProRule annotation
    Disulfide bondi210 ↔ 240PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Expressioni

    Tissue specificityi

    Pancreas.

    Gene expression databases

    GenevestigatoriP00773.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000006351.

    Structurei

    3D structure databases

    ProteinModelPortaliP00773.
    SMRiP00773. Positions 27-264.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 264238Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00640000091262.
    HOGENOMiHOG000251820.
    InParanoidiP00773.
    KOiK01326.
    OrthoDBiEOG7RRF7Z.
    PhylomeDBiP00773.
    TreeFamiTF330455.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00773-1 [UniParc]FASTAAdd to Basket

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    MLRFLVFASL VLYGHSTQDF PETNARVVGG AEARRNSWPS QISLQYLSGG    50
    SWYHTCGGTL IRRNWVMTAA HCVSSQMTFR VVVGDHNLSQ NDGTEQYVSV 100
    QKIMVHPTWN SNNVAAGYDI ALLRLAQSVT LNNYVQLAVL PQEGTILANN 150
    NPCYITGWGR TRTNGQLSQT LQQAYLPSVD YSICSSSSYW GSTVKTTMVC 200
    AGGDGVRSGC QGDSGGPLHC LVNGQYSVHG VTSFVSSMGC NVSKKPTVFT 250
    RVSAYISWMN NVIAYT 266
    Length:266
    Mass (Da):28,976
    Last modified:July 21, 1986 - v1
    Checksum:i5A56FE8FCF1AAEDA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti104 – 1041M → V in AAA98811. (PubMed:6094548)Curated
    Sequence conflicti108 – 1081T → N in AAA98811. (PubMed:6094548)Curated
    Sequence conflicti244 – 2441K → R in AAA98811. (PubMed:6094548)Curated
    Sequence conflicti266 – 2661T → N in AAA98811. (PubMed:6094548)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01234 mRNA. Translation: CAA24544.1.
    L00117
    , L00112, L00113, L00114, L00115, L00116 Genomic DNA. Translation: AAA98811.1.
    PIRiA00960. ELRT1.
    RefSeqiNP_036684.1. NM_012552.3.
    UniGeneiRn.6044.

    Genome annotation databases

    EnsembliENSRNOT00000006351; ENSRNOP00000006351; ENSRNOG00000004725.
    GeneIDi24331.
    KEGGirno:24331.
    UCSCiRGD:2547. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01234 mRNA. Translation: CAA24544.1 .
    L00117
    , L00112 , L00113 , L00114 , L00115 , L00116 Genomic DNA. Translation: AAA98811.1 .
    PIRi A00960. ELRT1.
    RefSeqi NP_036684.1. NM_012552.3.
    UniGenei Rn.6044.

    3D structure databases

    ProteinModelPortali P00773.
    SMRi P00773. Positions 27-264.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000006351.

    Protein family/group databases

    MEROPSi S01.153.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000006351 ; ENSRNOP00000006351 ; ENSRNOG00000004725 .
    GeneIDi 24331.
    KEGGi rno:24331.
    UCSCi RGD:2547. rat.

    Organism-specific databases

    CTDi 1990.
    RGDi 2547. Cela1.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00640000091262.
    HOGENOMi HOG000251820.
    InParanoidi P00773.
    KOi K01326.
    OrthoDBi EOG7RRF7Z.
    PhylomeDBi P00773.
    TreeFami TF330455.

    Miscellaneous databases

    NextBioi 603007.
    PROi P00773.

    Gene expression databases

    Genevestigatori P00773.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of two distinct rat pancreatic preproelastases determined by sequence analysis of the complete cloned messenger ribonucleic acid sequences."
      MacDonald R.J., Swift G.H., Quinto C., Swain W., Pictet R.L., Nikovits W., Rutter W.J.
      Biochemistry 21:1453-1463(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Structure of the two related elastase genes expressed in the rat pancreas."
      Swift G.H., Craik C.S., Stary S.J., Quinto C., Lahaie R.G., Rutter W.J., MacDonald R.J.
      J. Biol. Chem. 259:14271-14278(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Isolation and characterization of rat pancreatic elastase."
      Largman C.
      Biochemistry 22:3763-3770(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 17-45.
      Tissue: Pancreas.

    Entry informationi

    Entry nameiCELA1_RAT
    AccessioniPrimary (citable) accession number: P00773
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3