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P00773

- CELA1_RAT

UniProt

P00773 - CELA1_RAT

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Protein

Chymotrypsin-like elastase family member 1

Gene

Cela1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts upon elastin.

Catalytic activityi

Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.

Cofactori

Ca2+By similarityNote: Binds 1 Ca(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711Charge relay systemBy similarity
Metal bindingi85 – 851CalciumBy similarity
Metal bindingi90 – 901Calcium; via carbonyl oxygenBy similarity
Metal bindingi95 – 951CalciumBy similarity
Active sitei119 – 1191Charge relay systemBy similarity
Active sitei214 – 2141Charge relay systemBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. exocrine pancreas development Source: Ensembl
  2. inflammatory response Source: Ensembl
  3. multicellular organism growth Source: Ensembl
  4. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  5. pancreas morphogenesis Source: Ensembl
  6. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  7. post-embryonic development Source: Ensembl
  8. regulation of cell differentiation Source: Ensembl
  9. regulation of cell proliferation Source: Ensembl
  10. Wnt signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS01.153.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsin-like elastase family member 1 (EC:3.4.21.36)
Alternative name(s):
Elastase-1
Gene namesi
Name:Cela1
Synonyms:Ela1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi2547. Cela1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16161 PublicationAdd
BLAST
Propeptidei17 – 2610Activation peptidePRO_0000027683
Chaini27 – 266240Chymotrypsin-like elastase family member 1PRO_0000027684Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 72PROSITE-ProRule annotation
Disulfide bondi153 ↔ 220PROSITE-ProRule annotation
Disulfide bondi184 ↔ 200PROSITE-ProRule annotation
Disulfide bondi210 ↔ 240PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

GenevestigatoriP00773.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006351.

Structurei

3D structure databases

ProteinModelPortaliP00773.
SMRiP00773. Positions 27-264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 264238Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
InParanoidiP00773.
KOiK01326.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP00773.
TreeFamiTF330455.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00773-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRFLVFASL VLYGHSTQDF PETNARVVGG AEARRNSWPS QISLQYLSGG
60 70 80 90 100
SWYHTCGGTL IRRNWVMTAA HCVSSQMTFR VVVGDHNLSQ NDGTEQYVSV
110 120 130 140 150
QKIMVHPTWN SNNVAAGYDI ALLRLAQSVT LNNYVQLAVL PQEGTILANN
160 170 180 190 200
NPCYITGWGR TRTNGQLSQT LQQAYLPSVD YSICSSSSYW GSTVKTTMVC
210 220 230 240 250
AGGDGVRSGC QGDSGGPLHC LVNGQYSVHG VTSFVSSMGC NVSKKPTVFT
260
RVSAYISWMN NVIAYT
Length:266
Mass (Da):28,976
Last modified:July 21, 1986 - v1
Checksum:i5A56FE8FCF1AAEDA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1041M → V in AAA98811. (PubMed:6094548)Curated
Sequence conflicti108 – 1081T → N in AAA98811. (PubMed:6094548)Curated
Sequence conflicti244 – 2441K → R in AAA98811. (PubMed:6094548)Curated
Sequence conflicti266 – 2661T → N in AAA98811. (PubMed:6094548)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01234 mRNA. Translation: CAA24544.1.
L00117
, L00112, L00113, L00114, L00115, L00116 Genomic DNA. Translation: AAA98811.1.
PIRiA00960. ELRT1.
RefSeqiNP_036684.1. NM_012552.3.
UniGeneiRn.6044.

Genome annotation databases

EnsembliENSRNOT00000006351; ENSRNOP00000006351; ENSRNOG00000004725.
GeneIDi24331.
KEGGirno:24331.
UCSCiRGD:2547. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01234 mRNA. Translation: CAA24544.1 .
L00117
, L00112 , L00113 , L00114 , L00115 , L00116 Genomic DNA. Translation: AAA98811.1 .
PIRi A00960. ELRT1.
RefSeqi NP_036684.1. NM_012552.3.
UniGenei Rn.6044.

3D structure databases

ProteinModelPortali P00773.
SMRi P00773. Positions 27-264.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000006351.

Protein family/group databases

MEROPSi S01.153.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000006351 ; ENSRNOP00000006351 ; ENSRNOG00000004725 .
GeneIDi 24331.
KEGGi rno:24331.
UCSCi RGD:2547. rat.

Organism-specific databases

CTDi 1990.
RGDi 2547. Cela1.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00760000119027.
HOGENOMi HOG000251820.
InParanoidi P00773.
KOi K01326.
OrthoDBi EOG7RRF7Z.
PhylomeDBi P00773.
TreeFami TF330455.

Miscellaneous databases

NextBioi 603007.
PROi P00773.

Gene expression databases

Genevestigatori P00773.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of two distinct rat pancreatic preproelastases determined by sequence analysis of the complete cloned messenger ribonucleic acid sequences."
    MacDonald R.J., Swift G.H., Quinto C., Swain W., Pictet R.L., Nikovits W., Rutter W.J.
    Biochemistry 21:1453-1463(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structure of the two related elastase genes expressed in the rat pancreas."
    Swift G.H., Craik C.S., Stary S.J., Quinto C., Lahaie R.G., Rutter W.J., MacDonald R.J.
    J. Biol. Chem. 259:14271-14278(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Isolation and characterization of rat pancreatic elastase."
    Largman C.
    Biochemistry 22:3763-3770(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-45.
    Tissue: Pancreas.

Entry informationi

Entry nameiCELA1_RAT
AccessioniPrimary (citable) accession number: P00773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 26, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3