Reviewed, UniProtKB/Swiss-Prot P00773 (ELA1_RAT)

Names and origin

Protein names

Recommended name:
    Elastase-1
    EC=3.4.21.36

Gene names

Name:

Ela1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts upon elastin.
Catalytic activity	Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Ref.3
	Propeptide	17 – 26	10	Activation peptide		PRO_0000027683
	Chain	27 – 266	240	Elastase-1		PRO_0000027684
Regions
	Domain	27 – 264	238	Peptidase S1
Sites
	Active site	71	1	Charge relay system By similarity
	Active site	119	1	Charge relay system By similarity
	Active site	214	1	Charge relay system By similarity
	Metal binding	85	1	Calcium By similarity
	Metal binding	90	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	95	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	153 ↔ 220		By similarity
	Disulfide bond	184 ↔ 200		By similarity
	Disulfide bond	210 ↔ 240		By similarity
Experimental info
	Sequence conflict	104	1	M → V in AAA98811. Ref.2
	Sequence conflict	108	1	T → N in AAA98811. Ref.2
	Sequence conflict	244	1	K → R in AAA98811. Ref.2
	Sequence conflict	266	1	T → N in AAA98811. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00773-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 5A56FE8FCF1AAEDA	FASTA	266	28,976
	10 20 30 40 50 60 MLRFLVFASL VLYGHSTQDF PETNARVVGG AEARRNSWPS QISLQYLSGG SWYHTCGGTL 70 80 90 100 110 120 IRRNWVMTAA HCVSSQMTFR VVVGDHNLSQ NDGTEQYVSV QKIMVHPTWN SNNVAAGYDI 130 140 150 160 170 180 ALLRLAQSVT LNNYVQLAVL PQEGTILANN NPCYITGWGR TRTNGQLSQT LQQAYLPSVD 190 200 210 220 230 240 YSICSSSSYW GSTVKTTMVC AGGDGVRSGC QGDSGGPLHC LVNGQYSVHG VTSFVSSMGC 250 260 NVSKKPTVFT RVSAYISWMN NVIAYT

References

[1]	"Primary structure of two distinct rat pancreatic preproelastases determined by sequence analysis of the complete cloned messenger ribonucleic acid sequences." MacDonald R.J., Swift G.H., Quinto C., Swain W., Pictet R.L., Nikovits W., Rutter W.J. Biochemistry 21:1453-1463(1982) [PubMed: 6918221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure of the two related elastase genes expressed in the rat pancreas." Swift G.H., Craik C.S., Stary S.J., Quinto C., Lahaie R.G., Rutter W.J., MacDonald R.J. J. Biol. Chem. 259:14271-14278(1984) [PubMed: 6094548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Isolation and characterization of rat pancreatic elastase." Largman C. Biochemistry 22:3763-3770(1983) [PubMed: 6555050] [Abstract] Cited for: PROTEIN SEQUENCE OF 17-45. Tissue: Pancreas.

Cross-references

Sequence databases
EMBLGenBankDDBJ	V01234 mRNA. Translation: CAA24544.1. L00117 , L00112, L00113, L00114, L00115, L00116 Genomic DNA. Translation: AAA98811.1.
IPI	IPI00327729.
PIR	ELRT1. A00960.
UniGene	Rn.6044
3D structure databases
HSSP	HSSP built from PDB template 1QNJ based on UniProtKB P00772.
SMR	P00773. Positions 27-264.
ModBase	Search...
Protein family/group databases
MEROPS	S01.153.
Genome annotation databases
Ensembl	ENSRNOG00000004725. Rattus norvegicus. [Contig view]
Organism-specific databases
RGD	2547. Ela1.
Phylogenomic databases
HOVERGEN	P00773.
Enzyme and pathway databases
BRENDA	3.4.21.36. 248.
Gene expression databases
ArrayExpress	P00773.
GermOnline	ENSRNOG00000004725. Rattus norvegicus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ELA1_RAT

Accession

Primary (citable) accession number: P00773

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)