P00773 (CELA1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chymotrypsin-like elastase family member 1
EC=3.4.21.36

Alternative name(s):
Elastase-1

Gene names

Name:	Cela1
Synonyms:	Ela1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts upon elastin.
Catalytic activity	Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara exocrine pancreas development Inferred from electronic annotation. Source: Compara inflammatory response Inferred from electronic annotation. Source: Compara multicellular organism growth Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara pancreas morphogenesis Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara post-embryonic development Inferred from electronic annotation. Source: Compara proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell differentiation Inferred from electronic annotation. Source: Compara regulation of cell proliferation Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Ref.3
	Propeptide	17 – 26	10	Activation peptide		PRO_0000027683
	Chain	27 – 266	240	Chymotrypsin-like elastase family member 1		PRO_0000027684
Regions
	Domain	27 – 264	238	Peptidase S1
Sites
	Active site	71	1	Charge relay system By similarity
	Active site	119	1	Charge relay system By similarity
	Active site	214	1	Charge relay system By similarity
	Metal binding	85	1	Calcium By similarity
	Metal binding	90	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	95	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	153 ↔ 220		By similarity
	Disulfide bond	184 ↔ 200		By similarity
	Disulfide bond	210 ↔ 240		By similarity
Experimental info
	Sequence conflict	104	1	M → V in AAA98811. Ref.2
	Sequence conflict	108	1	T → N in AAA98811. Ref.2
	Sequence conflict	244	1	K → R in AAA98811. Ref.2
	Sequence conflict	266	1	T → N in AAA98811. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00773 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 5A56FE8FCF1AAEDA	FASTA	266	28,976
	10 20 30 40 50 60 MLRFLVFASL VLYGHSTQDF PETNARVVGG AEARRNSWPS QISLQYLSGG SWYHTCGGTL 70 80 90 100 110 120 IRRNWVMTAA HCVSSQMTFR VVVGDHNLSQ NDGTEQYVSV QKIMVHPTWN SNNVAAGYDI 130 140 150 160 170 180 ALLRLAQSVT LNNYVQLAVL PQEGTILANN NPCYITGWGR TRTNGQLSQT LQQAYLPSVD 190 200 210 220 230 240 YSICSSSSYW GSTVKTTMVC AGGDGVRSGC QGDSGGPLHC LVNGQYSVHG VTSFVSSMGC 250 260 NVSKKPTVFT RVSAYISWMN NVIAYT

References

[1]	"Primary structure of two distinct rat pancreatic preproelastases determined by sequence analysis of the complete cloned messenger ribonucleic acid sequences." MacDonald R.J., Swift G.H., Quinto C., Swain W., Pictet R.L., Nikovits W., Rutter W.J. Biochemistry 21:1453-1463(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure of the two related elastase genes expressed in the rat pancreas." Swift G.H., Craik C.S., Stary S.J., Quinto C., Lahaie R.G., Rutter W.J., MacDonald R.J. J. Biol. Chem. 259:14271-14278(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Isolation and characterization of rat pancreatic elastase." Largman C. Biochemistry 22:3763-3770(1983) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 17-45. Tissue: Pancreas.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V01234 mRNA. Translation: CAA24544.1. L00117 , L00112, L00113, L00114, L00115, L00116 Genomic DNA. Translation: AAA98811.1.
IPI	IPI00327729.
PIR	ELRT1. A00960.
RefSeq	NP_036684.1. NM_012552.3.
UniGene	Rn.6044.
3D structure databases
ProteinModelPortal	P00773.
SMR	P00773. Positions 27-264.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000006351.
Protein family/group databases
MEROPS	S01.153.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000006351; ENSRNOP00000006351; ENSRNOG00000004725.
GeneID	24331.
KEGG	rno:24331.
UCSC	RGD:2547. rat.
Organism-specific databases
CTD	1990.
RGD	2547. Cela1.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00640000091262.
HOGENOM	HOG000251820.
InParanoid	P00773.
KO	K01326.
OrthoDB	EOG4V171P.
Gene expression databases
Genevestigator	P00773.
GermOnline	ENSRNOG00000004725. Rattus norvegicus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603007.

Entry information

Entry name

CELA1_RAT

Accession

Primary (citable) accession number: P00773

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program