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P00770 (MCPT2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Mast cell protease 2

Short name=rMCP-2
EC=3.4.21.-
Alternative name(s):
Group-specific protease
Mast cell protease II
Short name=rMCP-II
Gene names
Name:Mcpt2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme, isolated from small intestine, specifically inactivates the apo forms of a certain group of intracellular pyridoxal phosphate-requiring enzymes. It has chymotrypsin-like specificity towards small substrates.

Sequence similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818
Propeptide19 – 202Activation peptide
PRO_0000027441
Chain21 – 247227Mast cell protease 2
PRO_0000027442

Regions

Domain21 – 244224Peptidase S1

Sites

Active site651Charge relay system
Active site1091Charge relay system
Active site2021Charge relay system

Amino acid modifications

Disulfide bond50 ↔ 66
Disulfide bond143 ↔ 208
Disulfide bond174 ↔ 187

Experimental info

Sequence conflict2381W → T AA sequence Ref.2

Secondary structure

.................................... 247
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00770 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 051988042A97A47E

FASTA24727,102
        10         20         30         40         50         60 
MQALLFLMAL LLPSGAGAEE IIGGVESIPH SRPYMAHLDI VTEKGLRVIC GGFLISRQFV 

        70         80         90        100        110        120 
LTAAHCKGRE ITVILGAHDV RKRESTQQKI KVEKQIIHES YNSVPNLHDI MLLKLEKKVE 

       130        140        150        160        170        180 
LTPAVNVVPL PSPSDFIHPG AMCWAAGWGK TGVRDPTSYT LREVELRIMD EKACVDYRYY 

       190        200        210        220        230        240 
EYKFQVCVGS PTTLRAAFMG DSGGPLLCAG VAHGIVSYGH PDAKPPAIFT RVSTYVPWIN 


AVINTSS 

« Hide

References

[1]"Cloning of the mast cell protease, RMCP II. Evidence for cell-specific expression and a multi-gene family."
Benfey P.N., Yin F.H., Leder P.
J. Biol. Chem. 262:5377-5384(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Covalent structure of a group-specific protease from rat small intestine. Appendix: crystallographic data for a group specific protease from rat intestine."
Woodbury R.G., Katunuma N., Kobayashi K., Titani K., Neurath H.
Biochemistry 17:811-819(1978) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-244.
[3]"The structure of rat mast cell protease II at 1.9-A resolution."
Remington S.J., Woodbury R.G., Reynolds R.A., Matthews B.W., Neurath H.
Biochemistry 27:8097-8105(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02712 Genomic DNA. Translation: AAA66284.1.
PIRPRRTG. A29548.
RefSeqNP_742041.1. NM_172044.1.
UniGeneRn.9443.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3RP2X-ray1.90A/B21-244[»]
ProteinModelPortalP00770.
SMRP00770. Positions 21-244.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000027988.

Protein family/group databases

MEROPSS01.141.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000027988; ENSRNOP00000027988; ENSRNOG00000020625.
GeneID29266.
KEGGrno:29266.
UCSCRGD:621058. rat.

Organism-specific databases

CTD17225.
RGD621058. Mcpt2.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00740000115279.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP00770.
KOK08662.
OMAPWINAVI.
OrthoDBEOG7RRF7Z.
PhylomeDBP00770.
TreeFamTF333630.

Gene expression databases

GenevestigatorP00770.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00770.
NextBio608604.

Entry information

Entry nameMCPT2_RAT
AccessionPrimary (citable) accession number: P00770
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references