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P00770

- MCPT2_RAT

UniProt

P00770 - MCPT2_RAT

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Protein
Mast cell protease 2
Gene
Mcpt2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This enzyme, isolated from small intestine, specifically inactivates the apo forms of a certain group of intracellular pyridoxal phosphate-requiring enzymes. It has chymotrypsin-like specificity towards small substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei109 – 1091Charge relay system
Active sitei202 – 2021Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: RefGenome
Complete GO annotation...

GO - Biological processi

  1. proteolysis Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.141.

Names & Taxonomyi

Protein namesi
Recommended name:
Mast cell protease 2 (EC:3.4.21.-)
Short name:
rMCP-2
Alternative name(s):
Group-specific protease
Mast cell protease II
Short name:
rMCP-II
Gene namesi
Name:Mcpt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 15

Organism-specific databases

RGDi621058. Mcpt2.

Subcellular locationi

GO - Cellular componenti

  1. secretory granule Source: RefGenome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818
Add
BLAST
Propeptidei19 – 202Activation peptide
PRO_0000027441
Chaini21 – 247227Mast cell protease 2
PRO_0000027442Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 66
Disulfide bondi143 ↔ 208
Disulfide bondi174 ↔ 187

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Gene expression databases

GenevestigatoriP00770.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027988.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi35 – 417
Beta strandi47 – 6216
Helixi64 – 663
Beta strandi69 – 768
Beta strandi88 – 9710
Beta strandi103 – 1053
Beta strandi111 – 1177
Beta strandi142 – 15211
Beta strandi155 – 1573
Beta strandi162 – 1698
Helixi171 – 1733
Turni174 – 1785
Turni182 – 1843
Beta strandi185 – 1884
Turni199 – 2035
Beta strandi205 – 2084
Beta strandi211 – 2188
Beta strandi227 – 2315
Helixi232 – 24312

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RP2X-ray1.90A/B21-244[»]
ProteinModelPortaliP00770.
SMRiP00770. Positions 21-244.

Miscellaneous databases

EvolutionaryTraceiP00770.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 244224Peptidase S1
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00740000115279.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00770.
KOiK08662.
OMAiPWINAVI.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP00770.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00770-1 [UniParc]FASTAAdd to Basket

« Hide

MQALLFLMAL LLPSGAGAEE IIGGVESIPH SRPYMAHLDI VTEKGLRVIC    50
GGFLISRQFV LTAAHCKGRE ITVILGAHDV RKRESTQQKI KVEKQIIHES 100
YNSVPNLHDI MLLKLEKKVE LTPAVNVVPL PSPSDFIHPG AMCWAAGWGK 150
TGVRDPTSYT LREVELRIMD EKACVDYRYY EYKFQVCVGS PTTLRAAFMG 200
DSGGPLLCAG VAHGIVSYGH PDAKPPAIFT RVSTYVPWIN AVINTSS 247
Length:247
Mass (Da):27,102
Last modified:January 1, 1988 - v1
Checksum:i051988042A97A47E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti238 – 2381W → T AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02712 Genomic DNA. Translation: AAA66284.1.
PIRiA29548. PRRTG.
RefSeqiNP_742041.1. NM_172044.1.
UniGeneiRn.9443.

Genome annotation databases

EnsembliENSRNOT00000027988; ENSRNOP00000027988; ENSRNOG00000020625.
GeneIDi29266.
KEGGirno:29266.
UCSCiRGD:621058. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02712 Genomic DNA. Translation: AAA66284.1 .
PIRi A29548. PRRTG.
RefSeqi NP_742041.1. NM_172044.1.
UniGenei Rn.9443.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3RP2 X-ray 1.90 A/B 21-244 [» ]
ProteinModelPortali P00770.
SMRi P00770. Positions 21-244.
ModBasei Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000027988.

Protein family/group databases

MEROPSi S01.141.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000027988 ; ENSRNOP00000027988 ; ENSRNOG00000020625 .
GeneIDi 29266.
KEGGi rno:29266.
UCSCi RGD:621058. rat.

Organism-specific databases

CTDi 17225.
RGDi 621058. Mcpt2.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00740000115279.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi P00770.
KOi K08662.
OMAi PWINAVI.
OrthoDBi EOG7RRF7Z.
PhylomeDBi P00770.
TreeFami TF333630.

Miscellaneous databases

EvolutionaryTracei P00770.
NextBioi 608604.

Gene expression databases

Genevestigatori P00770.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of the mast cell protease, RMCP II. Evidence for cell-specific expression and a multi-gene family."
    Benfey P.N., Yin F.H., Leder P.
    J. Biol. Chem. 262:5377-5384(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Covalent structure of a group-specific protease from rat small intestine. Appendix: crystallographic data for a group specific protease from rat intestine."
    Woodbury R.G., Katunuma N., Kobayashi K., Titani K., Neurath H.
    Biochemistry 17:811-819(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-244.
  3. "The structure of rat mast cell protease II at 1.9-A resolution."
    Remington S.J., Woodbury R.G., Reynolds R.A., Matthews B.W., Neurath H.
    Biochemistry 27:8097-8105(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Entry informationi

Entry nameiMCPT2_RAT
AccessioniPrimary (citable) accession number: P00770
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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