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P00770

- MCPT2_RAT

UniProt

P00770 - MCPT2_RAT

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Protein

Mast cell protease 2

Gene

Mcpt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This enzyme, isolated from small intestine, specifically inactivates the apo forms of a certain group of intracellular pyridoxal phosphate-requiring enzymes. It has chymotrypsin-like specificity towards small substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei109 – 1091Charge relay system
Active sitei202 – 2021Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.141.

Names & Taxonomyi

Protein namesi
Recommended name:
Mast cell protease 2 (EC:3.4.21.-)
Short name:
rMCP-2
Alternative name(s):
Group-specific protease
Mast cell protease II
Short name:
rMCP-II
Gene namesi
Name:Mcpt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 15

Organism-specific databases

RGDi621058. Mcpt2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Add
BLAST
Propeptidei19 – 202Activation peptide1 PublicationPRO_0000027441
Chaini21 – 247227Mast cell protease 2PRO_0000027442Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 66
Disulfide bondi143 ↔ 208
Disulfide bondi174 ↔ 187

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Gene expression databases

GenevestigatoriP00770.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027988.

Structurei

Secondary structure

1
247
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi35 – 417Combined sources
Beta strandi47 – 6216Combined sources
Helixi64 – 663Combined sources
Beta strandi69 – 768Combined sources
Beta strandi88 – 9710Combined sources
Beta strandi103 – 1053Combined sources
Beta strandi111 – 1177Combined sources
Beta strandi142 – 15211Combined sources
Beta strandi155 – 1573Combined sources
Beta strandi162 – 1698Combined sources
Helixi171 – 1733Combined sources
Turni174 – 1785Combined sources
Turni182 – 1843Combined sources
Beta strandi185 – 1884Combined sources
Turni199 – 2035Combined sources
Beta strandi205 – 2084Combined sources
Beta strandi211 – 2188Combined sources
Beta strandi227 – 2315Combined sources
Helixi232 – 24312Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RP2X-ray1.90A/B21-244[»]
ProteinModelPortaliP00770.
SMRiP00770. Positions 21-244.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00770.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 244224Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00770.
KOiK08662.
OMAiPWINAVI.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP00770.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00770-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQALLFLMAL LLPSGAGAEE IIGGVESIPH SRPYMAHLDI VTEKGLRVIC
60 70 80 90 100
GGFLISRQFV LTAAHCKGRE ITVILGAHDV RKRESTQQKI KVEKQIIHES
110 120 130 140 150
YNSVPNLHDI MLLKLEKKVE LTPAVNVVPL PSPSDFIHPG AMCWAAGWGK
160 170 180 190 200
TGVRDPTSYT LREVELRIMD EKACVDYRYY EYKFQVCVGS PTTLRAAFMG
210 220 230 240
DSGGPLLCAG VAHGIVSYGH PDAKPPAIFT RVSTYVPWIN AVINTSS
Length:247
Mass (Da):27,102
Last modified:January 1, 1988 - v1
Checksum:i051988042A97A47E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti238 – 2381W → T AA sequence (PubMed:629933)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02712 Genomic DNA. Translation: AAA66284.1.
PIRiA29548. PRRTG.
RefSeqiNP_742041.1. NM_172044.1.
UniGeneiRn.9443.

Genome annotation databases

EnsembliENSRNOT00000027988; ENSRNOP00000027988; ENSRNOG00000020625.
GeneIDi29266.
KEGGirno:29266.
UCSCiRGD:621058. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02712 Genomic DNA. Translation: AAA66284.1 .
PIRi A29548. PRRTG.
RefSeqi NP_742041.1. NM_172044.1.
UniGenei Rn.9443.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3RP2 X-ray 1.90 A/B 21-244 [» ]
ProteinModelPortali P00770.
SMRi P00770. Positions 21-244.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000027988.

Protein family/group databases

MEROPSi S01.141.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000027988 ; ENSRNOP00000027988 ; ENSRNOG00000020625 .
GeneIDi 29266.
KEGGi rno:29266.
UCSCi RGD:621058. rat.

Organism-specific databases

CTDi 17225.
RGDi 621058. Mcpt2.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00760000118895.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi P00770.
KOi K08662.
OMAi PWINAVI.
OrthoDBi EOG7RRF7Z.
PhylomeDBi P00770.
TreeFami TF333630.

Miscellaneous databases

EvolutionaryTracei P00770.
NextBioi 608604.

Gene expression databases

Genevestigatori P00770.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of the mast cell protease, RMCP II. Evidence for cell-specific expression and a multi-gene family."
    Benfey P.N., Yin F.H., Leder P.
    J. Biol. Chem. 262:5377-5384(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Covalent structure of a group-specific protease from rat small intestine. Appendix: crystallographic data for a group specific protease from rat intestine."
    Woodbury R.G., Katunuma N., Kobayashi K., Titani K., Neurath H.
    Biochemistry 17:811-819(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-244.
  3. "The structure of rat mast cell protease II at 1.9-A resolution."
    Remington S.J., Woodbury R.G., Reynolds R.A., Matthews B.W., Neurath H.
    Biochemistry 27:8097-8105(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Entry informationi

Entry nameiMCPT2_RAT
AccessioniPrimary (citable) accession number: P00770
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: November 26, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3