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Protein

Mast cell protease 2

Gene

Mcpt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme, isolated from small intestine, specifically inactivates the apo forms of a certain group of intracellular pyridoxal phosphate-requiring enzymes. It has chymotrypsin-like specificity towards small substrates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay system1
Active sitei109Charge relay system1
Active sitei202Charge relay system1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.141.

Names & Taxonomyi

Protein namesi
Recommended name:
Mast cell protease 2 (EC:3.4.21.-)
Short name:
rMCP-2
Alternative name(s):
Group-specific protease
Mast cell protease II
Short name:
rMCP-II
Gene namesi
Name:Mcpt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi621058. Mcpt2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Add BLAST18
PropeptideiPRO_000002744119 – 20Activation peptide1 Publication2
ChainiPRO_000002744221 – 247Mast cell protease 2Add BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 66
Disulfide bondi143 ↔ 208
Disulfide bondi174 ↔ 187

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP00770.

Expressioni

Gene expression databases

BgeeiENSRNOG00000020625.
ExpressionAtlasiP00770. baseline and differential.
GenevisibleiP00770. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027988.

Structurei

Secondary structure

1247
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi35 – 41Combined sources7
Beta strandi47 – 62Combined sources16
Helixi64 – 66Combined sources3
Beta strandi69 – 76Combined sources8
Beta strandi88 – 97Combined sources10
Beta strandi103 – 105Combined sources3
Beta strandi111 – 117Combined sources7
Beta strandi142 – 152Combined sources11
Beta strandi155 – 157Combined sources3
Beta strandi162 – 169Combined sources8
Helixi171 – 173Combined sources3
Turni174 – 178Combined sources5
Turni182 – 184Combined sources3
Beta strandi185 – 188Combined sources4
Turni199 – 203Combined sources5
Beta strandi205 – 208Combined sources4
Beta strandi211 – 218Combined sources8
Beta strandi227 – 231Combined sources5
Helixi232 – 243Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RP2X-ray1.90A/B21-244[»]
ProteinModelPortaliP00770.
SMRiP00770.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00770.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 244Peptidase S1PROSITE-ProRule annotationAdd BLAST224

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00770.
KOiK08662.
OMAiCKGREIT.
PhylomeDBiP00770.
TreeFamiTF333630.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00770-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQALLFLMAL LLPSGAGAEE IIGGVESIPH SRPYMAHLDI VTEKGLRVIC
60 70 80 90 100
GGFLISRQFV LTAAHCKGRE ITVILGAHDV RKRESTQQKI KVEKQIIHES
110 120 130 140 150
YNSVPNLHDI MLLKLEKKVE LTPAVNVVPL PSPSDFIHPG AMCWAAGWGK
160 170 180 190 200
TGVRDPTSYT LREVELRIMD EKACVDYRYY EYKFQVCVGS PTTLRAAFMG
210 220 230 240
DSGGPLLCAG VAHGIVSYGH PDAKPPAIFT RVSTYVPWIN AVINTSS
Length:247
Mass (Da):27,102
Last modified:January 1, 1988 - v1
Checksum:i051988042A97A47E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti238W → T AA sequence (PubMed:629933).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02712 Genomic DNA. Translation: AAA66284.1.
PIRiA29548. PRRTG.
RefSeqiNP_742041.1. NM_172044.1.
UniGeneiRn.9443.

Genome annotation databases

EnsembliENSRNOT00000027988; ENSRNOP00000027988; ENSRNOG00000020625.
ENSRNOT00000091757; ENSRNOP00000069470; ENSRNOG00000020625.
GeneIDi29266.
KEGGirno:29266.
UCSCiRGD:621058. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02712 Genomic DNA. Translation: AAA66284.1.
PIRiA29548. PRRTG.
RefSeqiNP_742041.1. NM_172044.1.
UniGeneiRn.9443.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RP2X-ray1.90A/B21-244[»]
ProteinModelPortaliP00770.
SMRiP00770.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027988.

Protein family/group databases

MEROPSiS01.141.

Proteomic databases

PaxDbiP00770.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027988; ENSRNOP00000027988; ENSRNOG00000020625.
ENSRNOT00000091757; ENSRNOP00000069470; ENSRNOG00000020625.
GeneIDi29266.
KEGGirno:29266.
UCSCiRGD:621058. rat.

Organism-specific databases

CTDi17225.
RGDi621058. Mcpt2.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00770.
KOiK08662.
OMAiCKGREIT.
PhylomeDBiP00770.
TreeFamiTF333630.

Miscellaneous databases

EvolutionaryTraceiP00770.
PROiP00770.

Gene expression databases

BgeeiENSRNOG00000020625.
ExpressionAtlasiP00770. baseline and differential.
GenevisibleiP00770. RN.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCPT2_RAT
AccessioniPrimary (citable) accession number: P00770
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.