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P00770

- MCPT2_RAT

UniProt

P00770 - MCPT2_RAT

Protein

Mast cell protease 2

Gene

Mcpt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    Functioni

    This enzyme, isolated from small intestine, specifically inactivates the apo forms of a certain group of intracellular pyridoxal phosphate-requiring enzymes. It has chymotrypsin-like specificity towards small substrates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651Charge relay system
    Active sitei109 – 1091Charge relay system
    Active sitei202 – 2021Charge relay system

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.141.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mast cell protease 2 (EC:3.4.21.-)
    Short name:
    rMCP-2
    Alternative name(s):
    Group-specific protease
    Mast cell protease II
    Short name:
    rMCP-II
    Gene namesi
    Name:Mcpt2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 15

    Organism-specific databases

    RGDi621058. Mcpt2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Add
    BLAST
    Propeptidei19 – 202Activation peptide1 PublicationPRO_0000027441
    Chaini21 – 247227Mast cell protease 2PRO_0000027442Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi50 ↔ 66
    Disulfide bondi143 ↔ 208
    Disulfide bondi174 ↔ 187

    Keywords - PTMi

    Disulfide bond, Zymogen

    Expressioni

    Gene expression databases

    GenevestigatoriP00770.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000027988.

    Structurei

    Secondary structure

    1
    247
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi35 – 417
    Beta strandi47 – 6216
    Helixi64 – 663
    Beta strandi69 – 768
    Beta strandi88 – 9710
    Beta strandi103 – 1053
    Beta strandi111 – 1177
    Beta strandi142 – 15211
    Beta strandi155 – 1573
    Beta strandi162 – 1698
    Helixi171 – 1733
    Turni174 – 1785
    Turni182 – 1843
    Beta strandi185 – 1884
    Turni199 – 2035
    Beta strandi205 – 2084
    Beta strandi211 – 2188
    Beta strandi227 – 2315
    Helixi232 – 24312

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RP2X-ray1.90A/B21-244[»]
    ProteinModelPortaliP00770.
    SMRiP00770. Positions 21-244.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00770.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 244224Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00740000115279.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP00770.
    KOiK08662.
    OMAiPWINAVI.
    OrthoDBiEOG7RRF7Z.
    PhylomeDBiP00770.
    TreeFamiTF333630.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00770-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQALLFLMAL LLPSGAGAEE IIGGVESIPH SRPYMAHLDI VTEKGLRVIC    50
    GGFLISRQFV LTAAHCKGRE ITVILGAHDV RKRESTQQKI KVEKQIIHES 100
    YNSVPNLHDI MLLKLEKKVE LTPAVNVVPL PSPSDFIHPG AMCWAAGWGK 150
    TGVRDPTSYT LREVELRIMD EKACVDYRYY EYKFQVCVGS PTTLRAAFMG 200
    DSGGPLLCAG VAHGIVSYGH PDAKPPAIFT RVSTYVPWIN AVINTSS 247
    Length:247
    Mass (Da):27,102
    Last modified:January 1, 1988 - v1
    Checksum:i051988042A97A47E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti238 – 2381W → T AA sequence (PubMed:629933)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02712 Genomic DNA. Translation: AAA66284.1.
    PIRiA29548. PRRTG.
    RefSeqiNP_742041.1. NM_172044.1.
    UniGeneiRn.9443.

    Genome annotation databases

    EnsembliENSRNOT00000027988; ENSRNOP00000027988; ENSRNOG00000020625.
    GeneIDi29266.
    KEGGirno:29266.
    UCSCiRGD:621058. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02712 Genomic DNA. Translation: AAA66284.1 .
    PIRi A29548. PRRTG.
    RefSeqi NP_742041.1. NM_172044.1.
    UniGenei Rn.9443.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3RP2 X-ray 1.90 A/B 21-244 [» ]
    ProteinModelPortali P00770.
    SMRi P00770. Positions 21-244.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000027988.

    Protein family/group databases

    MEROPSi S01.141.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027988 ; ENSRNOP00000027988 ; ENSRNOG00000020625 .
    GeneIDi 29266.
    KEGGi rno:29266.
    UCSCi RGD:621058. rat.

    Organism-specific databases

    CTDi 17225.
    RGDi 621058. Mcpt2.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00740000115279.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P00770.
    KOi K08662.
    OMAi PWINAVI.
    OrthoDBi EOG7RRF7Z.
    PhylomeDBi P00770.
    TreeFami TF333630.

    Miscellaneous databases

    EvolutionaryTracei P00770.
    NextBioi 608604.

    Gene expression databases

    Genevestigatori P00770.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the mast cell protease, RMCP II. Evidence for cell-specific expression and a multi-gene family."
      Benfey P.N., Yin F.H., Leder P.
      J. Biol. Chem. 262:5377-5384(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Covalent structure of a group-specific protease from rat small intestine. Appendix: crystallographic data for a group specific protease from rat intestine."
      Woodbury R.G., Katunuma N., Kobayashi K., Titani K., Neurath H.
      Biochemistry 17:811-819(1978) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-244.
    3. "The structure of rat mast cell protease II at 1.9-A resolution."
      Remington S.J., Woodbury R.G., Reynolds R.A., Matthews B.W., Neurath H.
      Biochemistry 27:8097-8105(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

    Entry informationi

    Entry nameiMCPT2_RAT
    AccessioniPrimary (citable) accession number: P00770
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3