Reviewed, UniProtKB/Swiss-Prot P00769 (CTR2_VESCR)

Names and origin

Protein names	Recommended name:     Chymotrypsin-2     EC=3.4.21.1 Alternative name(s):     Chymotrypsin II
Organism	Vespa crabro (European hornet)
Taxonomic identifier	7445 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Vespinae › Vespa

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.
Subcellular location	Secreted › extracellular space.
Miscellaneous	An additional Arg at the carboxyl end was found in some of the molecules.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Chymotrypsin-2		PRO_0000088676
Regions
	Domain	1 – 218	218	Peptidase S1
Sites
	Active site	39	1	Charge relay system
	Active site	84	1	Charge relay system
	Active site	175	1	Charge relay system
Amino acid modifications
	Disulfide bond	25 ↔ 40
	Disulfide bond	148 ↔ 161
	Disulfide bond	171 ↔ 195

Sequences

	Sequence		Length	Mass (Da)
	P00769-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 509AB50DE190EB39	FASTA	218	23,677
	10 20 30 40 50 60 IVGGTDAPRG KYPYQVSLRA PKHFCGGSIS KRYVLTAAHC LVGKSKHQVT VHAGSVLLNK 70 80 90 100 110 120 EEAVYNAEEL IVNKNYNSIR LINDIGLIRV SKDISYTQLV QPVKLPVSNT IKAGDPVVLT 130 140 150 160 170 180 GWGRIYVNGP IPNNLQQITL SIVNQQTCKF KHWGLTDSQI CTFTKLGEGA CDGDSGGPLV 190 200 210 ANGVQIGIVS YGHPCAVGSP NVFTRVYSFL DWIQKNQL

References

[1]	"Amino acid sequence of the chymotryptic protease II from the larvae of the nornet, Vespa crabro." Jany K.-D., Haug H. FEBS Lett. 158:98-102(1983) Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
PIR	KYVH2C. A00955.
3D structure databases
HSSP	HSSP built from PDB template 1GHW based on UniProtKB P00734.
ModBase	Search...
Protein family/group databases
MEROPS	S01.438.
Enzyme and pathway databases
BRENDA	3.4.21.1. 296866.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTR2_VESCR

Accession

Primary (citable) accession number: P00769

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)