Reviewed, UniProtKB/Swiss-Prot P00768 (CTR2_VESOR)

Names and origin

Protein names	Recommended name:     Chymotrypsin-2     EC=3.4.21.1 Alternative name(s):     Chymotrypsin II
Organism	Vespa orientalis (Oriental hornet)
Taxonomic identifier	7447 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Vespinae › Vespa

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	Chymotrypsin-2		PRO_0000088677
Regions
	Domain	1 – 216	216	Peptidase S1
Sites
	Active site	39	1	Charge relay system Ref.2
	Active site	82	1	Charge relay system Ref.2
	Active site	173	1	Charge relay system Ref.2
Amino acid modifications
	Disulfide bond	25 ↔ 40		Ref.1
	Disulfide bond	146 ↔ 159		Ref.1
	Disulfide bond	169 ↔ 193		Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P00768-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: F235BF992AEFEDE1	FASTA	216	23,471
	10 20 30 40 50 60 IVGGTNAPRG KYPYQVSLRA PKHFCGGSIS KRYVLTAAHC LVGKSEHQVT VGSVLLNKEE 70 80 90 100 110 120 AVYNAKELIV NKNYNSIRLI NDIGLIRVSK DISFTQLVQP VKLPVSNTIK AGDPVVLTGW 130 140 150 160 170 180 GRIYVNGPIP NNLQQITLSI VNQQTCKSKH WGLTDSQICT FTKRGEGACH GDSGGPLVAN 190 200 210 GVQIGIVSYG HPCAIGSPNV FTRVYSFLDW IQKNQL

References

[1]	"Amino acid sequence of an insect chymotrypsin from the larvae of the hornet, Vespa orientalis." Jany K.-D., Bekelar K., Pfleiderer G., Ishay J. Biochem. Biophys. Res. Commun. 110:1-7(1983) [PubMed: 6340663] [Abstract] Cited for: PROTEIN SEQUENCE, DISULFIDE BONDS.
[2]	"The amino acid sequences around the reactive serine and histidine residues of the chymotrypsin-like protease from the hornet, Vespa orientalis." Jany K.-D., Bekelar K., Ishay J. Biochim. Biophys. Acta 668:197-200(1981) [PubMed: 6786354] [Abstract] Cited for: ACTIVE SITE.

Cross-references

Sequence databases
PIR	KYVH2O. A00954.
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBase	Search...
Protein family/group databases
MEROPS	S01.438.
Enzyme and pathway databases
BRENDA	3.4.21.1. 290116.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTR2_VESOR

Accession

Primary (citable) accession number: P00768

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)