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Protein

Chymotrypsinogen B

Gene
N/A
Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei57 – 571Charge relay system1 Publication
Active sitei102 – 1021Charge relay system1 Publication
Active sitei195 – 1951Charge relay system1 Publication

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiS01.152.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsinogen B (EC:3.4.21.1)
Cleaved into the following 3 chains:
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1313Chymotrypsin B chain APRO_0000027629Add
BLAST
Propeptidei14 – 152PRO_0000027630
Chaini16 – 146131Chymotrypsin B chain BPRO_0000027631Add
BLAST
Propeptidei147 – 1482PRO_0000027632
Chaini149 – 24597Chymotrypsin B chain CPRO_0000027633Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi1 ↔ 1221 PublicationPROSITE-ProRule annotation
Disulfide bondi42 ↔ 581 PublicationPROSITE-ProRule annotation
Disulfide bondi136 ↔ 2011 PublicationPROSITE-ProRule annotation
Disulfide bondi168 ↔ 1821 PublicationPROSITE-ProRule annotation
Disulfide bondi191 ↔ 2201 PublicationPROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PRIDEiP00767.

Interactioni

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CBWX-ray2.60A/F1-13[»]
1HJAX-ray2.30A1-13[»]
3BG4X-ray2.50A1-13[»]
ProteinModelPortaliP00767.
SMRiP00767. Positions 1-245.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00767.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 243228Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5640.
HOVERGENiHBG013304.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00767-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
CGVPAIQPVL SGLARIVNGE DAVPGSWPWQ VSLQDSTGFH FCGGSLISED
60 70 80 90 100
WVVTAAHCGV TTSDVVVAGE FDQGLETEDT QVLKIGKVFK NPKFSILTVR
110 120 130 140 150
NDITLLKLAT PAQFSETVSA VCLPSADEDF PAGMLCATTG WGKTKYNALK
160 170 180 190 200
TPDKLQQATL PIVSNTDCRK YWGSRVTDVM ICAGASGVSS CMGDSGGPLV
210 220 230 240
CQKNGAWTLA GIVSWGSSTC STSTPAVYAR VTALMPWVQE TLAAN
Length:245
Mass (Da):25,755
Last modified:July 21, 1986 - v1
Checksum:i678016446FF5FEB5
GO

Sequence databases

PIRiA00953. KYBOB.
UniGeneiBt.107724.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

PIRiA00953. KYBOB.
UniGeneiBt.107724.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CBWX-ray2.60A/F1-13[»]
1HJAX-ray2.30A1-13[»]
3BG4X-ray2.50A1-13[»]
ProteinModelPortaliP00767.
SMRiP00767. Positions 1-245.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP00767.
ChEMBLiCHEMBL3063.

Protein family/group databases

MEROPSiS01.152.

Proteomic databases

PRIDEiP00767.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG5640.
HOVERGENiHBG013304.

Miscellaneous databases

EvolutionaryTraceiP00767.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structure of chymotrypsinogen B compared with chymotrypsinogen A and trypsinogen."
    Smillie L.B., Furka A., Nagabhushan N., Stevenson K.J., Parkes C.O.
    Nature 218:343-346(1968) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, DISULFIDE BONDS, ACTIVE SITE.

Entry informationi

Entry nameiCTRB_BOVIN
AccessioniPrimary (citable) accession number: P00767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: January 7, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.