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P00765

- TRYP_ASTAS

UniProt

P00765 - TRYP_ASTAS

Protein

Trypsin-1

Gene
N/A
Organism
Astacus astacus (Noble crayfish) (Astacus fluviatilis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    • Comment

    Functioni

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei45 – 451Charge relay systemBy similarity
    Metal bindingi64 – 641CalciumBy similarity
    Metal bindingi69 – 691Calcium; via carbonyl oxygenBy similarity
    Metal bindingi74 – 741CalciumBy similarity
    Active sitei96 – 961Charge relay systemBy similarity
    Sitei183 – 1831Required for specificityBy similarity
    Active sitei189 – 1891Charge relay systemBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    MEROPSiS01.035.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin-1 (EC:3.4.21.4)
    Alternative name(s):
    Trypsin I
    OrganismiAstacus astacus (Noble crayfish) (Astacus fluviatilis)
    Taxonomic identifieri6715 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaCrustaceaMalacostracaEumalacostracaEucaridaDecapodaPleocyemataAstacideaAstacoideaAstacidaeAstacus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 237237Trypsin-1PRO_0000088715Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi30 ↔ 46
    Disulfide bondi159 ↔ 174
    Disulfide bondi185 ↔ 213

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliP00765.
    SMRiP00765. Positions 1-236.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 237237Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00765-1 [UniParc]FASTAAdd to Basket

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    IVGGTDAVLG EFPYQLSFQE TFLGFSFHFC GASIYNENYA ITAGHCVYGD    50
    DYENPSGLQI VAGELDMSVN EGSEQTITVS KIILHENFDY DLLDNDISLL 100
    KLSGSLTFNN NVAPIALPAQ GHTATGNVIV TGWGTTSEGG NTPDVLQKVT 150
    VPLVSDAECR DDYGADEIFD SMICAGVPEG GKDSCQGDSG GPLAASDTGS 200
    TYLAGIVSWG YGCARPGYPG VYTEVSYHVD WIKANAV 237
    Length:237
    Mass (Da):25,022
    Last modified:July 21, 1986 - v1
    Checksum:i4072133E55022C76
    GO

    Sequence databases

    PIRiA00951. TRCY1.

    Cross-referencesi

    Sequence databases

    PIRi A00951. TRCY1.

    3D structure databases

    ProteinModelPortali P00765.
    SMRi P00765. Positions 1-236.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.035.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: PROTEIN SEQUENCE.

    Entry informationi

    Entry nameiTRYP_ASTAS
    AccessioniPrimary (citable) accession number: P00765
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    Trypsin I is one of five forms of the enzyme known to be present in crayfish. This protein is more acidic than mammalian trypsin.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3