P00765 (TRYP_ASTFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Trypsin-1 EC=3.4.21.4 Alternative name(s): Trypsin I
Organism	Astacus fluviatilis (Broad-fingered crayfish) (Astacus astacus)
Taxonomic identifier	6715 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Crustacea › Malacostraca › Eumalacostraca › Eucarida › Decapoda › Pleocyemata › Astacidea › Astacoidea › Astacidae › Astacus

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Miscellaneous	Trypsin I is one of five forms of the enzyme known to be present in crayfish. This protein is more acidic than mammalian trypsin.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	Trypsin-1		PRO_0000088715
Regions
	Domain	1 – 237	237	Peptidase S1
Sites
	Active site	45	1	Charge relay system By similarity
	Active site	96	1	Charge relay system By similarity
	Active site	189	1	Charge relay system By similarity
	Metal binding	64	1	Calcium By similarity
	Metal binding	69	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	74	1	Calcium By similarity
	Site	183	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	30 ↔ 46
	Disulfide bond	159 ↔ 174
	Disulfide bond	185 ↔ 213

Sequences

	Sequence		Length	Mass (Da)
	P00765 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 4072133E55022C76	FASTA	237	25,022
	10 20 30 40 50 60 IVGGTDAVLG EFPYQLSFQE TFLGFSFHFC GASIYNENYA ITAGHCVYGD DYENPSGLQI 70 80 90 100 110 120 VAGELDMSVN EGSEQTITVS KIILHENFDY DLLDNDISLL KLSGSLTFNN NVAPIALPAQ 130 140 150 160 170 180 GHTATGNVIV TGWGTTSEGG NTPDVLQKVT VPLVSDAECR DDYGADEIFD SMICAGVPEG 190 200 210 220 230 GKDSCQGDSG GPLAASDTGS TYLAGIVSWG YGCARPGYPG VYTEVSYHVD WIKANAV

References

[1]	"Amino acid sequence of crayfish (Astacus fluviatilis) trypsin If." Titani K., Sasagawa T., Woodbury R.G., Ericsson L.H., Dorsam H., Kraemer M., Neurath H., Zwilling R. Biochemistry 22:1459-1465(1983) [PubMed: 6838862] [Abstract] Cited for: PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	TRCY1. A00951.
3D structure databases
ProteinModelPortal	P00765.
SMR	P00765. Positions 1-236.
ModBase	Search...
Protein family/group databases
MEROPS	S01.035.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYP_ASTFL

Accession

Primary (citable) accession number: P00765

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 16, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)