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P00765

- TRYP_ASTAS

UniProt

P00765 - TRYP_ASTAS

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Protein

Trypsin-1

Gene
N/A
Organism
Astacus astacus (Noble crayfish) (Astacus fluviatilis)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Binds 1 calcium ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451Charge relay system By similarity
Metal bindingi64 – 641Calcium By similarity
Metal bindingi69 – 691Calcium; via carbonyl oxygen By similarity
Metal bindingi74 – 741Calcium By similarity
Active sitei96 – 961Charge relay system By similarity
Sitei183 – 1831Required for specificity By similarity
Active sitei189 – 1891Charge relay system By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS01.035.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-1 (EC:3.4.21.4)
Alternative name(s):
Trypsin I
OrganismiAstacus astacus (Noble crayfish) (Astacus fluviatilis)
Taxonomic identifieri6715 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaCrustaceaMalacostracaEumalacostracaEucaridaDecapodaPleocyemataAstacideaAstacoideaAstacidaeAstacus

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237Trypsin-1PRO_0000088715Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 46
Disulfide bondi159 ↔ 174
Disulfide bondi185 ↔ 213

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP00765.
SMRiP00765. Positions 1-236.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 237237Peptidase S1Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00765-1 [UniParc]FASTAAdd to Basket

« Hide

IVGGTDAVLG EFPYQLSFQE TFLGFSFHFC GASIYNENYA ITAGHCVYGD    50
DYENPSGLQI VAGELDMSVN EGSEQTITVS KIILHENFDY DLLDNDISLL 100
KLSGSLTFNN NVAPIALPAQ GHTATGNVIV TGWGTTSEGG NTPDVLQKVT 150
VPLVSDAECR DDYGADEIFD SMICAGVPEG GKDSCQGDSG GPLAASDTGS 200
TYLAGIVSWG YGCARPGYPG VYTEVSYHVD WIKANAV 237
Length:237
Mass (Da):25,022
Last modified:July 21, 1986 - v1
Checksum:i4072133E55022C76
GO

Sequence databases

PIRiA00951. TRCY1.

Cross-referencesi

Sequence databases

PIRi A00951. TRCY1.

3D structure databases

ProteinModelPortali P00765.
SMRi P00765. Positions 1-236.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.035.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: PROTEIN SEQUENCE.

Entry informationi

Entry nameiTRYP_ASTAS
AccessioniPrimary (citable) accession number: P00765
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Trypsin I is one of five forms of the enzyme known to be present in crayfish. This protein is more acidic than mammalian trypsin.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi