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Protein

Trypsin-1

Gene
N/A
Organism
Astacus astacus (Noble crayfish) (Astacus fluviatilis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451Charge relay systemBy similarity
Metal bindingi64 – 641CalciumBy similarity
Metal bindingi69 – 691Calcium; via carbonyl oxygenBy similarity
Metal bindingi74 – 741CalciumBy similarity
Active sitei96 – 961Charge relay systemBy similarity
Sitei183 – 1831Required for specificityBy similarity
Active sitei189 – 1891Charge relay systemBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS01.035.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-1 (EC:3.4.21.4)
Alternative name(s):
Trypsin I
OrganismiAstacus astacus (Noble crayfish) (Astacus fluviatilis)
Taxonomic identifieri6715 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaCrustaceaMalacostracaEumalacostracaEucaridaDecapodaPleocyemataAstacideaAstacoideaAstacidaeAstacus

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237Trypsin-1PRO_0000088715Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 46
Disulfide bondi159 ↔ 174
Disulfide bondi185 ↔ 213

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP00765.
SMRiP00765. Positions 1-236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 237237Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00765-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
IVGGTDAVLG EFPYQLSFQE TFLGFSFHFC GASIYNENYA ITAGHCVYGD
60 70 80 90 100
DYENPSGLQI VAGELDMSVN EGSEQTITVS KIILHENFDY DLLDNDISLL
110 120 130 140 150
KLSGSLTFNN NVAPIALPAQ GHTATGNVIV TGWGTTSEGG NTPDVLQKVT
160 170 180 190 200
VPLVSDAECR DDYGADEIFD SMICAGVPEG GKDSCQGDSG GPLAASDTGS
210 220 230
TYLAGIVSWG YGCARPGYPG VYTEVSYHVD WIKANAV
Length:237
Mass (Da):25,022
Last modified:July 21, 1986 - v1
Checksum:i4072133E55022C76
GO

Sequence databases

PIRiA00951. TRCY1.

Cross-referencesi

Sequence databases

PIRiA00951. TRCY1.

3D structure databases

ProteinModelPortaliP00765.
SMRiP00765. Positions 1-236.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.035.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: PROTEIN SEQUENCE.

Entry informationi

Entry nameiTRYP_ASTAS
AccessioniPrimary (citable) accession number: P00765
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: January 7, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Trypsin I is one of five forms of the enzyme known to be present in crayfish. This protein is more acidic than mammalian trypsin.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.