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P00765 (TRYP_ASTAS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trypsin-1

EC=3.4.21.4
Alternative name(s):
Trypsin I
OrganismAstacus astacus (Noble crayfish) (Astacus fluviatilis)
Taxonomic identifier6715 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaCrustaceaMalacostracaEumalacostracaEucaridaDecapodaPleocyemataAstacideaAstacoideaAstacidaeAstacus

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subcellular location

Secretedextracellular space.

Miscellaneous

Trypsin I is one of five forms of the enzyme known to be present in crayfish. This protein is more acidic than mammalian trypsin.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Biological processDigestion
   Cellular componentSecreted
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processdigestion

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

serine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 237237Trypsin-1
PRO_0000088715

Regions

Domain1 – 237237Peptidase S1

Sites

Active site451Charge relay system By similarity
Active site961Charge relay system By similarity
Active site1891Charge relay system By similarity
Metal binding641Calcium By similarity
Metal binding691Calcium; via carbonyl oxygen By similarity
Metal binding741Calcium By similarity
Site1831Required for specificity By similarity

Amino acid modifications

Disulfide bond30 ↔ 46
Disulfide bond159 ↔ 174
Disulfide bond185 ↔ 213

Sequences

Sequence LengthMass (Da)Tools
P00765 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 4072133E55022C76

FASTA23725,022
        10         20         30         40         50         60 
IVGGTDAVLG EFPYQLSFQE TFLGFSFHFC GASIYNENYA ITAGHCVYGD DYENPSGLQI 

        70         80         90        100        110        120 
VAGELDMSVN EGSEQTITVS KIILHENFDY DLLDNDISLL KLSGSLTFNN NVAPIALPAQ 

       130        140        150        160        170        180 
GHTATGNVIV TGWGTTSEGG NTPDVLQKVT VPLVSDAECR DDYGADEIFD SMICAGVPEG 

       190        200        210        220        230 
GKDSCQGDSG GPLAASDTGS TYLAGIVSWG YGCARPGYPG VYTEVSYHVD WIKANAV 

« Hide

References

[1]"Amino acid sequence of crayfish (Astacus fluviatilis) trypsin If."
Titani K., Sasagawa T., Woodbury R.G., Ericsson L.H., Dorsam H., Kraemer M., Neurath H., Zwilling R.
Biochemistry 22:1459-1465(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases

PIRTRCY1. A00951.

3D structure databases

ProteinModelPortalP00765.
SMRP00765. Positions 1-236.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.035.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRYP_ASTAS
AccessionPrimary (citable) accession number: P00765
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries