Reviewed,
UniProtKB/Swiss-Prot P00764 (TRYP_SQUAC)
Last modified
June 16, 2009.
Version 67.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Trypsin EC=3.4.21.4 |
| Organism | Squalus acanthias (Spiny dogfish) |
| Taxonomic identifier | 7797 [NCBI] |
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Chondrichthyes › Elasmobranchii › Squalea › Hypnosqualea › Squaliformes › Squaloidei › Squalidae › Squalus |
Protein attributes
| Sequence length | 229 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. |
| Cofactor | Binds 1 calcium ion per subunit By similarity. |
| Subcellular location | |
| Sequence similarities | Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Digestion |
| Cellular component | Secreted |
| Ligand | Calcium Metal-binding |
| Molecular function | Hydrolase Protease Serine protease |
| PTM | Disulfide bond Zymogen |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysisInferred from electronic annotation. Source: InterPro |
| Cellular component | extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Propeptide | 1 – 7 | 7 | Activation peptide Ref.1 | PRO_0000028231 | |||||||
| Chain | 8 – 229 | 222 | Trypsin | PRO_0000028232 | |||||||
Regions | |||||||||||
| Domain | 8 – 227 | 220 | Peptidase S1 | ||||||||
Sites | |||||||||||
| Active site | 47 | 1 | Charge relay system By similarity | ||||||||
| Active site | 91 | 1 | Charge relay system By similarity | ||||||||
| Active site | 183 | 1 | Charge relay system By similarity | ||||||||
| Metal binding | 59 | 1 | Calcium By similarity | ||||||||
| Metal binding | 69 | 1 | Calcium By similarity | ||||||||
| Site | 177 | 1 | Required for specificity By similarity | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 14 ↔ 143 | By similarity | |||||||||
| Disulfide bond | 32 ↔ 48 | By similarity | |||||||||
| Disulfide bond | 116 ↔ 216 | By similarity | |||||||||
| Disulfide bond | 123 ↔ 189 | By similarity | |||||||||
| Disulfide bond | 154 ↔ 168 | By similarity | |||||||||
| Disulfide bond | 179 ↔ 203 | By similarity | |||||||||
Natural variations | |||||||||||
| Natural variant | 109 | 1 | L → P | ||||||||
Sequences
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References
| [1] | "Amino acid sequence of dogfish trypsin." Titani K., Ericsson L.H., Neurath H., Walsh K.A. Biochemistry 14:1358-1366(1975) [PubMed: 1092332] [Abstract] Cited for: PROTEIN SEQUENCE OF 8-229. |
| [2] | "Comparison of the amino terminal sequences of bovine, dogfish, and lungfish trypsinogens." Hermodson M.A., Tye R.W., Reeck G.R., Neurath H., Walsh K.A. FEBS Lett. 14:222-224(1971) [PubMed: 11945763] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-21. |
Cross-references
Sequence databases | |
|---|---|
| PIR | TRDFS. A00950. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1EZX based on UniProtKB P00760. |
| ModBase | Search... |
Phylogenomic databases | |
| HOVERGEN | P00764. |
Enzyme and pathway databases | |
| BRENDA | 3.4.21.4. 981. |
Family and domain databases | |
| InterPro | IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view] |
| Pfam | PF00089. Trypsin. 1 hit. [Graphical view] |
| PRINTS | PR00722. CHYMOTRYPSIN. |
| SMART | SM00020. Tryp_SPc. 1 hit. [Graphical view] |
| PROSITE | PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | TRYP_SQUAC | ||||||||
| Accession | Primary (citable) accession number: P00764 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


