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P00764

- TRYP_SQUAC

UniProt

P00764 - TRYP_SQUAC

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Protein

Trypsin

Gene
N/A
Organism
Squalus acanthias (Spiny dogfish)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Binds 1 calcium ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei47 – 471Charge relay systemBy similarity
Metal bindingi59 – 591CalciumBy similarity
Metal bindingi69 – 691CalciumBy similarity
Active sitei91 – 911Charge relay systemBy similarity
Sitei177 – 1771Required for specificityBy similarity
Active sitei183 – 1831Charge relay systemBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin (EC:3.4.21.4)
OrganismiSqualus acanthias (Spiny dogfish)
Taxonomic identifieri7797 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiSqualimorphiiSqualiformesSqualidaeSqualus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 77Activation peptide1 PublicationPRO_0000028231
Chaini8 – 229222TrypsinPRO_0000028232Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi14 ↔ 143PROSITE-ProRule annotation
Disulfide bondi32 ↔ 48PROSITE-ProRule annotation
Disulfide bondi116 ↔ 216PROSITE-ProRule annotation
Disulfide bondi123 ↔ 189PROSITE-ProRule annotation
Disulfide bondi154 ↔ 168PROSITE-ProRule annotation
Disulfide bondi179 ↔ 203PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP00764.
SMRiP00764. Positions 8-227.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 227220Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00764-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
APDDDDKIVG GYECPKHAAP WTVSLNVGYH FCGGSLIAPG WVVSAAHCYQ
60 70 80 90 100
RRIQVRLGEH DISANEGDET YIDSSMVIRH PNYSGYDLDN DIMLIKLSKP
110 120 130 140 150
AALNRNVDLI SLPTGCAYAG EMCLISGWGN TMDGAVSGDQ LQCLDAPVLS
160 170 180 190 200
DAECKGAYPG MITNNMMCVG YMEGGKDSCQ GDSGGPVVCN GMLQGIVSWG
210 220
YGCAERDHPG VYTRVCHYVS WIHETIASV
Length:229
Mass (Da):24,591
Last modified:July 21, 1986 - v1
Checksum:iE83B83C5AD72FCE4
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti109 – 1091L → P.

Sequence databases

PIRiA00950. TRDFS.

Cross-referencesi

Sequence databases

PIRi A00950. TRDFS.

3D structure databases

ProteinModelPortali P00764.
SMRi P00764. Positions 8-227.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG013304.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: PROTEIN SEQUENCE OF 8-229.
  2. "Comparison of the amino terminal sequences of bovine, dogfish, and lungfish trypsinogens."
    Hermodson M.A., Tye R.W., Reeck G.R., Neurath H., Walsh K.A.
    FEBS Lett. 14:222-224(1971) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-21.

Entry informationi

Entry nameiTRYP_SQUAC
AccessioniPrimary (citable) accession number: P00764
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3