Reviewed, UniProtKB/Swiss-Prot P00764 (TRYP_SQUAC)

Names and origin

Protein names	Recommended name:     Trypsin     EC=3.4.21.4
Organism	Squalus acanthias (Spiny dogfish)
Taxonomic identifier	7797 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Chondrichthyes › Elasmobranchii › Squalea › Hypnosqualea › Squaliformes › Squaloidei › Squalidae › Squalus

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 7	7	Activation peptide Ref.1		PRO_0000028231
	Chain	8 – 229	222	Trypsin		PRO_0000028232
Regions
	Domain	8 – 227	220	Peptidase S1
Sites
	Active site	47	1	Charge relay system By similarity
	Active site	91	1	Charge relay system By similarity
	Active site	183	1	Charge relay system By similarity
	Metal binding	59	1	Calcium By similarity
	Metal binding	69	1	Calcium By similarity
	Site	177	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	14 ↔ 143		By similarity
	Disulfide bond	32 ↔ 48		By similarity
	Disulfide bond	116 ↔ 216		By similarity
	Disulfide bond	123 ↔ 189		By similarity
	Disulfide bond	154 ↔ 168		By similarity
	Disulfide bond	179 ↔ 203		By similarity
Natural variations
	Natural variant	109	1	L → P

Sequences

	Sequence		Length	Mass (Da)
	P00764-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: E83B83C5AD72FCE4	FASTA	229	24,591
	10 20 30 40 50 60 APDDDDKIVG GYECPKHAAP WTVSLNVGYH FCGGSLIAPG WVVSAAHCYQ RRIQVRLGEH 70 80 90 100 110 120 DISANEGDET YIDSSMVIRH PNYSGYDLDN DIMLIKLSKP AALNRNVDLI SLPTGCAYAG 130 140 150 160 170 180 EMCLISGWGN TMDGAVSGDQ LQCLDAPVLS DAECKGAYPG MITNNMMCVG YMEGGKDSCQ 190 200 210 220 GDSGGPVVCN GMLQGIVSWG YGCAERDHPG VYTRVCHYVS WIHETIASV

References

[1]	"Amino acid sequence of dogfish trypsin." Titani K., Ericsson L.H., Neurath H., Walsh K.A. Biochemistry 14:1358-1366(1975) [PubMed: 1092332] [Abstract] Cited for: PROTEIN SEQUENCE OF 8-229.
[2]	"Comparison of the amino terminal sequences of bovine, dogfish, and lungfish trypsinogens." Hermodson M.A., Tye R.W., Reeck G.R., Neurath H., Walsh K.A. FEBS Lett. 14:222-224(1971) [PubMed: 11945763] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-21.

Cross-references

Sequence databases
PIR	TRDFS. A00950.
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P00764.
Enzyme and pathway databases
BRENDA	3.4.21.4. 981.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYP_SQUAC

Accession

Primary (citable) accession number: P00764

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)