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P00764

- TRYP_SQUAC

UniProt

P00764 - TRYP_SQUAC

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Protein
Trypsin
Gene
N/A
Organism
Squalus acanthias (Spiny dogfish)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Binds 1 calcium ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei47 – 471Charge relay system By similarity
Metal bindingi59 – 591Calcium By similarity
Metal bindingi69 – 691Calcium By similarity
Active sitei91 – 911Charge relay system By similarity
Sitei177 – 1771Required for specificity By similarity
Active sitei183 – 1831Charge relay system By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin (EC:3.4.21.4)
OrganismiSqualus acanthias (Spiny dogfish)
Taxonomic identifieri7797 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiSqualimorphiiSqualiformesSqualidaeSqualus

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 77Activation peptide
PRO_0000028231
Chaini8 – 229222Trypsin
PRO_0000028232Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi14 ↔ 143 By similarity
Disulfide bondi32 ↔ 48 By similarity
Disulfide bondi116 ↔ 216 By similarity
Disulfide bondi123 ↔ 189 By similarity
Disulfide bondi154 ↔ 168 By similarity
Disulfide bondi179 ↔ 203 By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP00764.
SMRiP00764. Positions 8-227.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 227220Peptidase S1
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00764-1 [UniParc]FASTAAdd to Basket

« Hide

APDDDDKIVG GYECPKHAAP WTVSLNVGYH FCGGSLIAPG WVVSAAHCYQ    50
RRIQVRLGEH DISANEGDET YIDSSMVIRH PNYSGYDLDN DIMLIKLSKP 100
AALNRNVDLI SLPTGCAYAG EMCLISGWGN TMDGAVSGDQ LQCLDAPVLS 150
DAECKGAYPG MITNNMMCVG YMEGGKDSCQ GDSGGPVVCN GMLQGIVSWG 200
YGCAERDHPG VYTRVCHYVS WIHETIASV 229
Length:229
Mass (Da):24,591
Last modified:July 21, 1986 - v1
Checksum:iE83B83C5AD72FCE4
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti109 – 1091L → P.

Sequence databases

PIRiA00950. TRDFS.

Cross-referencesi

Sequence databases

PIRi A00950. TRDFS.

3D structure databases

ProteinModelPortali P00764.
SMRi P00764. Positions 8-227.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG013304.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: PROTEIN SEQUENCE OF 8-229.
  2. "Comparison of the amino terminal sequences of bovine, dogfish, and lungfish trypsinogens."
    Hermodson M.A., Tye R.W., Reeck G.R., Neurath H., Walsh K.A.
    FEBS Lett. 14:222-224(1971) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-21.

Entry informationi

Entry nameiTRYP_SQUAC
AccessioniPrimary (citable) accession number: P00764
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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