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P00763

- TRY2_RAT

UniProt

P00763 - TRY2_RAT

Protein

Anionic trypsin-2

Gene

Prss2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Cofactori

    Binds 1 calcium ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei63 – 631Charge relay system
    Metal bindingi75 – 751Calcium
    Metal bindingi77 – 771Calcium; via carbonyl oxygen
    Metal bindingi80 – 801Calcium; via carbonyl oxygen
    Metal bindingi85 – 851Calcium
    Active sitei107 – 1071Charge relay system
    Sitei194 – 1941Required for specificityBy similarity
    Active sitei200 – 2001Charge relay system

    GO - Molecular functioni

    1. calcium ion binding Source: UniProtKB
    2. protein binding Source: IntAct
    3. serine-type endopeptidase activity Source: UniProtKB

    GO - Biological processi

    1. collagen catabolic process Source: UniProtKB
    2. digestion Source: RGD
    3. proteolysis Source: UniProtKB
    4. response to nutrient Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198590. Activation of Matrix Metalloproteinases.
    REACT_199179. Alpha-defensins.
    SABIO-RKP00763.

    Protein family/group databases

    MEROPSiS01.119.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anionic trypsin-2 (EC:3.4.21.4)
    Alternative name(s):
    Anionic trypsin II
    Pretrypsinogen II
    Serine protease 2
    Gene namesi
    Name:Prss2
    Synonyms:Try2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi3418. Prss2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB
    2. extracellular space Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1515Add
    BLAST
    Propeptidei16 – 238Activation peptidePRO_0000028209
    Chaini24 – 246223Anionic trypsin-2PRO_0000028210Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi30 ↔ 160
    Disulfide bondi48 ↔ 64
    Disulfide bondi132 ↔ 233
    Disulfide bondi139 ↔ 206
    Disulfide bondi171 ↔ 185
    Disulfide bondi196 ↔ 220

    Keywords - PTMi

    Disulfide bond, Zymogen

    Proteomic databases

    PaxDbiP00763.
    PRIDEiP00763.

    PTM databases

    PhosphoSiteiP00763.

    Expressioni

    Gene expression databases

    GenevestigatoriP00763.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ecoP238272EBI-1029166,EBI-1029159From a different organism.

    Protein-protein interaction databases

    DIPiDIP-6112N.
    IntActiP00763. 2 interactions.
    MINTiMINT-202469.
    STRINGi10116.ENSRNOP00000062302.

    Structurei

    Secondary structure

    1
    246
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni32 – 343
    Beta strandi38 – 5417
    Beta strandi57 – 604
    Helixi62 – 643
    Beta strandi70 – 745
    Beta strandi76 – 805
    Beta strandi86 – 9510
    Turni101 – 1033
    Beta strandi109 – 1157
    Beta strandi120 – 1234
    Beta strandi138 – 1458
    Beta strandi149 – 1513
    Beta strandi159 – 1657
    Helixi168 – 1747
    Turni176 – 1783
    Beta strandi183 – 1875
    Beta strandi192 – 1943
    Turni197 – 2015
    Beta strandi203 – 2064
    Beta strandi209 – 2168
    Beta strandi218 – 2214
    Beta strandi227 – 2315
    Helixi232 – 2343
    Helixi236 – 24510

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AMHX-ray2.50A/B24-246[»]
    1ANBX-ray2.80A24-246[»]
    1ANCX-ray2.20A24-246[»]
    1ANDX-ray2.30A24-246[»]
    1ANEX-ray2.20A24-246[»]
    1BRAX-ray2.20A24-246[»]
    1BRBX-ray2.10E24-246[»]
    1BRCX-ray2.50E24-246[»]
    1CO7X-ray1.90E2-246[»]
    1DPOX-ray1.59A24-246[»]
    1EZSX-ray2.30C/D24-246[»]
    1EZUX-ray2.40C/D24-246[»]
    1F5RX-ray1.65A15-246[»]
    1F7ZX-ray1.55A15-246[»]
    1FY8X-ray1.70E15-246[»]
    1J14X-ray2.40A24-246[»]
    1J15X-ray2.00A24-246[»]
    1J16X-ray1.60A24-246[»]
    1J17X-ray2.00T24-246[»]
    1K9OX-ray2.30E24-246[»]
    1QL9X-ray2.30A24-246[»]
    1SLUX-ray1.80B24-246[»]
    1SLVX-ray2.30B24-246[»]
    1SLWX-ray2.00B24-246[»]
    1SLXX-ray2.20B24-246[»]
    1TRMX-ray2.30A/B24-246[»]
    1YKTX-ray1.70A24-246[»]
    1YLCX-ray1.70A24-246[»]
    1YLDX-ray1.70A24-239[»]
    2TRMX-ray2.80A24-246[»]
    3FP6X-ray1.49E24-246[»]
    3FP7X-ray1.46E24-246[»]
    3FP8X-ray1.46E24-246[»]
    3TGIX-ray1.80E24-246[»]
    3TGJX-ray2.20E15-246[»]
    3TGKX-ray1.70E15-237[»]
    ProteinModelPortaliP00763.
    SMRiP00763. Positions 24-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00763.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 244221Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00740000115192.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP00763.
    KOiK01312.
    OMAiGHCYKSA.
    OrthoDBiEOG75B84T.
    PhylomeDBiP00763.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00763-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRALLFLALV GAAVAFPVDD DDKIVGGYTC QENSVPYQVS LNSGYHFCGG    50
    SLINDQWVVS AAHCYKSRIQ VRLGEHNINV LEGNEQFVNA AKIIKHPNFD 100
    RKTLNNDIML IKLSSPVKLN ARVATVALPS SCAPAGTQCL ISGWGNTLSS 150
    GVNEPDLLQC LDAPLLPQAD CEASYPGKIT DNMVCVGFLE GGKDSCQGDS 200
    GGPVVCNGEL QGIVSWGYGC ALPDNPGVYT KVCNYVDWIQ DTIAAN 246
    Length:246
    Mass (Da):26,228
    Last modified:July 15, 1998 - v2
    Checksum:iA8D3630809AEE606
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti84 – 841N → D(PubMed:6094547)Curated
    Sequence conflicti88 – 881V → I(PubMed:6094547)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01274 mRNA. Translation: CAA24581.1.
    L00131, L00130 Genomic DNA. Translation: AAA98517.1.
    PIRiA22657. TRRT2.
    RefSeqiNP_036861.1. NM_012729.1.
    XP_003749781.1. XM_003749733.1.
    UniGeneiRn.1584.

    Genome annotation databases

    EnsembliENSRNOT00000018852; ENSRNOP00000018853; ENSRNOG00000032916.
    ENSRNOT00000066789; ENSRNOP00000060784; ENSRNOG00000049576.
    GeneIDi100912089.
    25052.
    KEGGirno:100912089.
    rno:25052.
    UCSCiRGD:3418. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01274 mRNA. Translation: CAA24581.1 .
    L00131 , L00130 Genomic DNA. Translation: AAA98517.1 .
    PIRi A22657. TRRT2.
    RefSeqi NP_036861.1. NM_012729.1.
    XP_003749781.1. XM_003749733.1.
    UniGenei Rn.1584.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AMH X-ray 2.50 A/B 24-246 [» ]
    1ANB X-ray 2.80 A 24-246 [» ]
    1ANC X-ray 2.20 A 24-246 [» ]
    1AND X-ray 2.30 A 24-246 [» ]
    1ANE X-ray 2.20 A 24-246 [» ]
    1BRA X-ray 2.20 A 24-246 [» ]
    1BRB X-ray 2.10 E 24-246 [» ]
    1BRC X-ray 2.50 E 24-246 [» ]
    1CO7 X-ray 1.90 E 2-246 [» ]
    1DPO X-ray 1.59 A 24-246 [» ]
    1EZS X-ray 2.30 C/D 24-246 [» ]
    1EZU X-ray 2.40 C/D 24-246 [» ]
    1F5R X-ray 1.65 A 15-246 [» ]
    1F7Z X-ray 1.55 A 15-246 [» ]
    1FY8 X-ray 1.70 E 15-246 [» ]
    1J14 X-ray 2.40 A 24-246 [» ]
    1J15 X-ray 2.00 A 24-246 [» ]
    1J16 X-ray 1.60 A 24-246 [» ]
    1J17 X-ray 2.00 T 24-246 [» ]
    1K9O X-ray 2.30 E 24-246 [» ]
    1QL9 X-ray 2.30 A 24-246 [» ]
    1SLU X-ray 1.80 B 24-246 [» ]
    1SLV X-ray 2.30 B 24-246 [» ]
    1SLW X-ray 2.00 B 24-246 [» ]
    1SLX X-ray 2.20 B 24-246 [» ]
    1TRM X-ray 2.30 A/B 24-246 [» ]
    1YKT X-ray 1.70 A 24-246 [» ]
    1YLC X-ray 1.70 A 24-246 [» ]
    1YLD X-ray 1.70 A 24-239 [» ]
    2TRM X-ray 2.80 A 24-246 [» ]
    3FP6 X-ray 1.49 E 24-246 [» ]
    3FP7 X-ray 1.46 E 24-246 [» ]
    3FP8 X-ray 1.46 E 24-246 [» ]
    3TGI X-ray 1.80 E 24-246 [» ]
    3TGJ X-ray 2.20 E 15-246 [» ]
    3TGK X-ray 1.70 E 15-237 [» ]
    ProteinModelPortali P00763.
    SMRi P00763. Positions 24-246.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6112N.
    IntActi P00763. 2 interactions.
    MINTi MINT-202469.
    STRINGi 10116.ENSRNOP00000062302.

    Chemistry

    BindingDBi P00763.
    ChEMBLi CHEMBL4290.

    Protein family/group databases

    MEROPSi S01.119.

    PTM databases

    PhosphoSitei P00763.

    Proteomic databases

    PaxDbi P00763.
    PRIDEi P00763.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000018852 ; ENSRNOP00000018853 ; ENSRNOG00000032916 .
    ENSRNOT00000066789 ; ENSRNOP00000060784 ; ENSRNOG00000049576 .
    GeneIDi 100912089.
    25052.
    KEGGi rno:100912089.
    rno:25052.
    UCSCi RGD:3418. rat.

    Organism-specific databases

    CTDi 5645.
    RGDi 3418. Prss2.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00740000115192.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P00763.
    KOi K01312.
    OMAi GHCYKSA.
    OrthoDBi EOG75B84T.
    PhylomeDBi P00763.

    Enzyme and pathway databases

    Reactomei REACT_198590. Activation of Matrix Metalloproteinases.
    REACT_199179. Alpha-defensins.
    SABIO-RK P00763.

    Miscellaneous databases

    EvolutionaryTracei P00763.
    NextBioi 605250.

    Gene expression databases

    Genevestigatori P00763.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of two related rat pancreatic trypsin genes."
      Craik C.S., Choo Q.L., Swift G.H., Quinto C., MacDonald R.J., Rutter W.J.
      J. Biol. Chem. 259:14255-14264(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Two similar but nonallelic rat pancreatic trypsinogens. Nucleotide sequences of the cloned cDNAs."
      MacDonald R.J., Stary S.J., Swift G.H.
      J. Biol. Chem. 257:9724-9732(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 9-246.
      Strain: Sprague-Dawley.
      Tissue: Pancreas.
    3. "The three-dimensional structure of Asn102 mutant of trypsin: role of Asp102 in serine protease catalysis."
      Sprang S., Standing T., Fletterick R.J., Stroud R.M., Finer-Moore J., Xuong N.-H., Hamlin R., Rutter W.J., Craik C.S.
      Science 237:905-909(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    4. "1.59-A structure of trypsin at 120 K: comparison of low temperature and room temperature structures."
      Earnest T., Fauman E., Craik C.S., Stroud R.
      Proteins 10:171-187(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.59 ANGSTROMS).
    5. "X-ray structures of a designed binding site in trypsin show metal-dependent geometry."
      Brinen L.S., Willett W.S., Craik C.S., Fletterick R.J.
      Biochemistry 35:5999-6009(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

    Entry informationi

    Entry nameiTRY2_RAT
    AccessioniPrimary (citable) accession number: P00763
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 147 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3