P00762 (TRY1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anionic trypsin-1
EC=3.4.21.4

Alternative name(s):
Anionic trypsin I
Pretrypsinogen I
Serine protease 1

Gene names

Name:	Prss1
Synonyms:	Try1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit.
Subcellular location	Secreted › extracellular space.
Miscellaneous	This sequence represents the precursor of the major form of trypsin produced by the adult pancreas.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	digestion Traceable author statement Ref.1. Source: RGD proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to caffeine Inferred from direct assay PubMed 2726780. Source: RGD response to nicotine Inferred from direct assay PubMed 2460970. Source: RGD response to nutrient Inferred from expression pattern PubMed 2363685. Source: RGD
Cellular_component	extracellular space Inferred from direct assay PubMed 3833185. Source: RGD
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	By similarity
	Propeptide	16 – 23	8	Activation peptide		PRO_0000028207
	Chain	24 – 246	223	Anionic trypsin-1		PRO_0000028208
Regions
	Domain	24 – 244	221	Peptidase S1
Sites
	Active site	63	1	Charge relay system
	Active site	107	1	Charge relay system
	Active site	200	1	Charge relay system
	Metal binding	75	1	Calcium
	Metal binding	77	1	Calcium; via carbonyl oxygen
	Metal binding	80	1	Calcium; via carbonyl oxygen
	Metal binding	85	1	Calcium
	Site	194	1	Required for specificity
Amino acid modifications
	Disulfide bond	30 ↔ 160		By similarity
	Disulfide bond	48 ↔ 64		By similarity
	Disulfide bond	132 ↔ 233		By similarity
	Disulfide bond	139 ↔ 206		By similarity
	Disulfide bond	171 ↔ 185		By similarity
	Disulfide bond	196 ↔ 220		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00762 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 6AFA0DAD11943FB5	FASTA	246	25,959
	10 20 30 40 50 60 MSALLILALV GAAVAFPLED DDKIVGGYTC PEHSVPYQVS LNSGYHFCGG SLINDQWVVS 70 80 90 100 110 120 AAHCYKSRIQ VRLGEHNINV LEGDEQFINA AKIIKHPNYS SWTLNNDIML IKLSSPVKLN 130 140 150 160 170 180 ARVAPVALPS ACAPAGTQCL ISGWGNTLSN GVNNPDLLQC VDAPVLSQAD CEAAYPGEIT 190 200 210 220 230 240 SSMICVGFLE GGKDSCQGDS GGPVVCNGQL QGIVSWGYGC ALPDNPGVYT KVCNFVGWIQ DTIAAN

References

[1]	"Two similar but nonallelic rat pancreatic trypsinogens. Nucleotide sequences of the cloned cDNAs." MacDonald R.J., Stary S.J., Swift G.H. J. Biol. Chem. 257:9724-9732(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley.
[2]	"Structure of two related rat pancreatic trypsin genes." Craik C.S., Choo Q.L., Swift G.H., Quinto C., MacDonald R.J., Rutter W.J. J. Biol. Chem. 259:14255-14264(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Lubec G., Kang S.U., Lubec S., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 73-92 AND 96-112, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V01273 mRNA. Translation: CAA24580.1. J00778 Genomic DNA. Translation: AAA98518.1.
IPI	IPI00212767.
PIR	TRRT1. B22657.
RefSeq	NP_036767.1. NM_012635.1.
UniGene	Rn.34435.
3D structure databases
ProteinModelPortal	P00762.
SMR	P00762. Positions 24-246.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000043068.
Protein family/group databases
MEROPS	S01.094.
PTM databases
PhosphoSite	P00762.
Proteomic databases
PaxDb	P00762.
PRIDE	P00762.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000040403; ENSRNOP00000043068; ENSRNOG00000032156.
GeneID	24691.
KEGG	rno:24691.
UCSC	RGD:3417. rat.
Organism-specific databases
CTD	5644.
RGD	3417. Prss1.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00680000099557.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P00762.
KO	K01312.
OMA	DLLQCVD.
OrthoDB	EOG4SJ5FV.
Gene expression databases
Genevestigator	P00762.
GermOnline	ENSRNOG00000032156. Rattus norvegicus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	604133.

Entry information

Entry name

TRY1_RAT

Accession

Primary (citable) accession number: P00762

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program