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Protein

Anionic trypsin-1

Gene

Prss1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Ca2+Note: Binds 1 Ca2+ ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei63 – 631Charge relay system
Metal bindingi75 – 751Calcium
Metal bindingi77 – 771Calcium; via carbonyl oxygen
Metal bindingi80 – 801Calcium; via carbonyl oxygen
Metal bindingi85 – 851Calcium
Active sitei107 – 1071Charge relay system
Sitei194 – 1941Required for specificity
Active sitei200 – 2001Charge relay system

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: RGD
  2. response to caffeine Source: RGD
  3. response to nicotine Source: RGD
  4. response to nutrient Source: RGD
  5. response to organic substance Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198590. Activation of Matrix Metalloproteinases.
REACT_199179. Alpha-defensins.

Protein family/group databases

MEROPSiS01.094.

Names & Taxonomyi

Protein namesi
Recommended name:
Anionic trypsin-1 (EC:3.4.21.4)
Alternative name(s):
Anionic trypsin I
Pretrypsinogen I
Serine protease 1
Gene namesi
Name:Prss1
Synonyms:Try1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi3417. Prss1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515By similarityAdd
BLAST
Propeptidei16 – 238Activation peptidePRO_0000028207
Chaini24 – 246223Anionic trypsin-1PRO_0000028208Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 160PROSITE-ProRule annotation
Disulfide bondi48 ↔ 64PROSITE-ProRule annotation
Disulfide bondi132 ↔ 233PROSITE-ProRule annotation
Disulfide bondi139 ↔ 206PROSITE-ProRule annotation
Disulfide bondi171 ↔ 185PROSITE-ProRule annotation
Disulfide bondi196 ↔ 220PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP00762.
PRIDEiP00762.

PTM databases

PhosphoSiteiP00762.

Expressioni

Gene expression databases

GenevestigatoriP00762.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043068.

Structurei

3D structure databases

ProteinModelPortaliP00762.
SMRiP00762. Positions 24-246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 244221Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00762.
KOiK01312.
OMAiTCPEHSV.
OrthoDBiEOG75B84T.
PhylomeDBiP00762.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00762-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSALLILALV GAAVAFPLED DDKIVGGYTC PEHSVPYQVS LNSGYHFCGG
60 70 80 90 100
SLINDQWVVS AAHCYKSRIQ VRLGEHNINV LEGDEQFINA AKIIKHPNYS
110 120 130 140 150
SWTLNNDIML IKLSSPVKLN ARVAPVALPS ACAPAGTQCL ISGWGNTLSN
160 170 180 190 200
GVNNPDLLQC VDAPVLSQAD CEAAYPGEIT SSMICVGFLE GGKDSCQGDS
210 220 230 240
GGPVVCNGQL QGIVSWGYGC ALPDNPGVYT KVCNFVGWIQ DTIAAN
Length:246
Mass (Da):25,959
Last modified:July 21, 1986 - v1
Checksum:i6AFA0DAD11943FB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01273 mRNA. Translation: CAA24580.1.
J00778 Genomic DNA. Translation: AAA98518.1.
PIRiB22657. TRRT1.
RefSeqiNP_036767.1. NM_012635.1.
UniGeneiRn.34435.

Genome annotation databases

EnsembliENSRNOT00000040403; ENSRNOP00000043068; ENSRNOG00000032156.
GeneIDi24691.
KEGGirno:24691.
UCSCiRGD:3417. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01273 mRNA. Translation: CAA24580.1.
J00778 Genomic DNA. Translation: AAA98518.1.
PIRiB22657. TRRT1.
RefSeqiNP_036767.1. NM_012635.1.
UniGeneiRn.34435.

3D structure databases

ProteinModelPortaliP00762.
SMRiP00762. Positions 24-246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043068.

Chemistry

BindingDBiP00762.

Protein family/group databases

MEROPSiS01.094.

PTM databases

PhosphoSiteiP00762.

Proteomic databases

PaxDbiP00762.
PRIDEiP00762.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000040403; ENSRNOP00000043068; ENSRNOG00000032156.
GeneIDi24691.
KEGGirno:24691.
UCSCiRGD:3417. rat.

Organism-specific databases

CTDi5644.
RGDi3417. Prss1.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00762.
KOiK01312.
OMAiTCPEHSV.
OrthoDBiEOG75B84T.
PhylomeDBiP00762.
TreeFamiTF331065.

Enzyme and pathway databases

ReactomeiREACT_198590. Activation of Matrix Metalloproteinases.
REACT_199179. Alpha-defensins.

Miscellaneous databases

NextBioi604133.
PROiP00762.

Gene expression databases

GenevestigatoriP00762.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Two similar but nonallelic rat pancreatic trypsinogens. Nucleotide sequences of the cloned cDNAs."
    MacDonald R.J., Stary S.J., Swift G.H.
    J. Biol. Chem. 257:9724-9732(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  2. "Structure of two related rat pancreatic trypsin genes."
    Craik C.S., Choo Q.L., Swift G.H., Quinto C., MacDonald R.J., Rutter W.J.
    J. Biol. Chem. 259:14255-14264(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Lubec G., Kang S.U., Lubec S., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 73-92 AND 96-112, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Hippocampus.

Entry informationi

Entry nameiTRY1_RAT
AccessioniPrimary (citable) accession number: P00762
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: January 7, 2015
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This sequence represents the precursor of the major form of trypsin produced by the adult pancreas.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.