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P00762 (TRY1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anionic trypsin-1

EC=3.4.21.4
Alternative name(s):
Anionic trypsin I
Pretrypsinogen I
Serine protease 1
Gene names
Name:Prss1
Synonyms:Try1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactor

Binds 1 calcium ion per subunit.

Subcellular location

Secretedextracellular space.

Miscellaneous

This sequence represents the precursor of the major form of trypsin produced by the adult pancreas.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515 By similarity
Propeptide16 – 238Activation peptide
PRO_0000028207
Chain24 – 246223Anionic trypsin-1
PRO_0000028208

Regions

Domain24 – 244221Peptidase S1

Sites

Active site631Charge relay system
Active site1071Charge relay system
Active site2001Charge relay system
Metal binding751Calcium
Metal binding771Calcium; via carbonyl oxygen
Metal binding801Calcium; via carbonyl oxygen
Metal binding851Calcium
Site1941Required for specificity

Amino acid modifications

Disulfide bond30 ↔ 160 By similarity
Disulfide bond48 ↔ 64 By similarity
Disulfide bond132 ↔ 233 By similarity
Disulfide bond139 ↔ 206 By similarity
Disulfide bond171 ↔ 185 By similarity
Disulfide bond196 ↔ 220 By similarity

Sequences

Sequence LengthMass (Da)Tools
P00762 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 6AFA0DAD11943FB5

FASTA24625,959
        10         20         30         40         50         60 
MSALLILALV GAAVAFPLED DDKIVGGYTC PEHSVPYQVS LNSGYHFCGG SLINDQWVVS 

        70         80         90        100        110        120 
AAHCYKSRIQ VRLGEHNINV LEGDEQFINA AKIIKHPNYS SWTLNNDIML IKLSSPVKLN 

       130        140        150        160        170        180 
ARVAPVALPS ACAPAGTQCL ISGWGNTLSN GVNNPDLLQC VDAPVLSQAD CEAAYPGEIT 

       190        200        210        220        230        240 
SSMICVGFLE GGKDSCQGDS GGPVVCNGQL QGIVSWGYGC ALPDNPGVYT KVCNFVGWIQ 


DTIAAN 

« Hide

References

[1]"Two similar but nonallelic rat pancreatic trypsinogens. Nucleotide sequences of the cloned cDNAs."
MacDonald R.J., Stary S.J., Swift G.H.
J. Biol. Chem. 257:9724-9732(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
[2]"Structure of two related rat pancreatic trypsin genes."
Craik C.S., Choo Q.L., Swift G.H., Quinto C., MacDonald R.J., Rutter W.J.
J. Biol. Chem. 259:14255-14264(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]Lubec G., Kang S.U., Lubec S., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 73-92 AND 96-112, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain and Hippocampus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V01273 mRNA. Translation: CAA24580.1.
J00778 Genomic DNA. Translation: AAA98518.1.
PIRTRRT1. B22657.
RefSeqNP_036767.1. NM_012635.1.
UniGeneRn.34435.

3D structure databases

ProteinModelPortalP00762.
SMRP00762. Positions 24-246.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000043068.

Protein family/group databases

MEROPSS01.094.

PTM databases

PhosphoSiteP00762.

Proteomic databases

PaxDbP00762.
PRIDEP00762.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000040403; ENSRNOP00000043068; ENSRNOG00000032156.
GeneID24691.
KEGGrno:24691.
UCSCRGD:3417. rat.

Organism-specific databases

CTD5644.
RGD3417. Prss1.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00740000115192.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP00762.
KOK01312.
OMANWIEETI.
OrthoDBEOG75B84T.
PhylomeDBP00762.
TreeFamTF331065.

Gene expression databases

GenevestigatorP00762.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio604133.
PROP00762.

Entry information

Entry nameTRY1_RAT
AccessionPrimary (citable) accession number: P00762
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries