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P00762

- TRY1_RAT

UniProt

P00762 - TRY1_RAT

Protein

Anionic trypsin-1

Gene

Prss1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Cofactori

    Binds 1 calcium ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei63 – 631Charge relay system
    Metal bindingi75 – 751Calcium
    Metal bindingi77 – 771Calcium; via carbonyl oxygen
    Metal bindingi80 – 801Calcium; via carbonyl oxygen
    Metal bindingi85 – 851Calcium
    Active sitei107 – 1071Charge relay system
    Sitei194 – 1941Required for specificity
    Active sitei200 – 2001Charge relay system

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: RGD
    2. response to caffeine Source: RGD
    3. response to nicotine Source: RGD
    4. response to nutrient Source: RGD
    5. response to organic substance Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198590. Activation of Matrix Metalloproteinases.
    REACT_199179. Alpha-defensins.

    Protein family/group databases

    MEROPSiS01.094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anionic trypsin-1 (EC:3.4.21.4)
    Alternative name(s):
    Anionic trypsin I
    Pretrypsinogen I
    Serine protease 1
    Gene namesi
    Name:Prss1
    Synonyms:Try1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi3417. Prss1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: RGD

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1515By similarityAdd
    BLAST
    Propeptidei16 – 238Activation peptidePRO_0000028207
    Chaini24 – 246223Anionic trypsin-1PRO_0000028208Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi30 ↔ 160PROSITE-ProRule annotation
    Disulfide bondi48 ↔ 64PROSITE-ProRule annotation
    Disulfide bondi132 ↔ 233PROSITE-ProRule annotation
    Disulfide bondi139 ↔ 206PROSITE-ProRule annotation
    Disulfide bondi171 ↔ 185PROSITE-ProRule annotation
    Disulfide bondi196 ↔ 220PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Proteomic databases

    PaxDbiP00762.
    PRIDEiP00762.

    PTM databases

    PhosphoSiteiP00762.

    Expressioni

    Gene expression databases

    GenevestigatoriP00762.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000043068.

    Structurei

    3D structure databases

    ProteinModelPortaliP00762.
    SMRiP00762. Positions 24-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 244221Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00740000115192.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP00762.
    KOiK01312.
    OMAiNWIEETI.
    OrthoDBiEOG75B84T.
    PhylomeDBiP00762.
    TreeFamiTF331065.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00762-1 [UniParc]FASTAAdd to Basket

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    MSALLILALV GAAVAFPLED DDKIVGGYTC PEHSVPYQVS LNSGYHFCGG    50
    SLINDQWVVS AAHCYKSRIQ VRLGEHNINV LEGDEQFINA AKIIKHPNYS 100
    SWTLNNDIML IKLSSPVKLN ARVAPVALPS ACAPAGTQCL ISGWGNTLSN 150
    GVNNPDLLQC VDAPVLSQAD CEAAYPGEIT SSMICVGFLE GGKDSCQGDS 200
    GGPVVCNGQL QGIVSWGYGC ALPDNPGVYT KVCNFVGWIQ DTIAAN 246
    Length:246
    Mass (Da):25,959
    Last modified:July 21, 1986 - v1
    Checksum:i6AFA0DAD11943FB5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01273 mRNA. Translation: CAA24580.1.
    J00778 Genomic DNA. Translation: AAA98518.1.
    PIRiB22657. TRRT1.
    RefSeqiNP_036767.1. NM_012635.1.
    UniGeneiRn.34435.

    Genome annotation databases

    EnsembliENSRNOT00000040403; ENSRNOP00000043068; ENSRNOG00000032156.
    GeneIDi24691.
    KEGGirno:24691.
    UCSCiRGD:3417. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01273 mRNA. Translation: CAA24580.1 .
    J00778 Genomic DNA. Translation: AAA98518.1 .
    PIRi B22657. TRRT1.
    RefSeqi NP_036767.1. NM_012635.1.
    UniGenei Rn.34435.

    3D structure databases

    ProteinModelPortali P00762.
    SMRi P00762. Positions 24-246.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000043068.

    Protein family/group databases

    MEROPSi S01.094.

    PTM databases

    PhosphoSitei P00762.

    Proteomic databases

    PaxDbi P00762.
    PRIDEi P00762.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000040403 ; ENSRNOP00000043068 ; ENSRNOG00000032156 .
    GeneIDi 24691.
    KEGGi rno:24691.
    UCSCi RGD:3417. rat.

    Organism-specific databases

    CTDi 5644.
    RGDi 3417. Prss1.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00740000115192.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P00762.
    KOi K01312.
    OMAi NWIEETI.
    OrthoDBi EOG75B84T.
    PhylomeDBi P00762.
    TreeFami TF331065.

    Enzyme and pathway databases

    Reactomei REACT_198590. Activation of Matrix Metalloproteinases.
    REACT_199179. Alpha-defensins.

    Miscellaneous databases

    NextBioi 604133.
    PROi P00762.

    Gene expression databases

    Genevestigatori P00762.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two similar but nonallelic rat pancreatic trypsinogens. Nucleotide sequences of the cloned cDNAs."
      MacDonald R.J., Stary S.J., Swift G.H.
      J. Biol. Chem. 257:9724-9732(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
    2. "Structure of two related rat pancreatic trypsin genes."
      Craik C.S., Choo Q.L., Swift G.H., Quinto C., MacDonald R.J., Rutter W.J.
      J. Biol. Chem. 259:14255-14264(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Lubec G., Kang S.U., Lubec S., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 73-92 AND 96-112, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain and Hippocampus.

    Entry informationi

    Entry nameiTRY1_RAT
    AccessioniPrimary (citable) accession number: P00762
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This sequence represents the precursor of the major form of trypsin produced by the adult pancreas.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3