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Protein

Anionic trypsin-1

Gene

Prss1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

This sequence represents the precursor of the major form of trypsin produced by the adult pancreas.

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Ca2+Note: Binds 1 Ca2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63Charge relay system1
Metal bindingi75Calcium1
Metal bindingi77Calcium; via carbonyl oxygen1
Metal bindingi80Calcium; via carbonyl oxygen1
Metal bindingi85Calcium1
Active sitei107Charge relay system1
Sitei194Required for specificity1
Active sitei200Charge relay system1

GO - Molecular functioni

GO - Biological processi

  • digestion Source: RGD
  • proteolysis Source: GO_Central
  • response to caffeine Source: RGD
  • response to nicotine Source: RGD
  • response to nutrient Source: RGD
  • response to organic substance Source: RGD

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processDigestion
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-1442490 Collagen degradation
R-RNO-1592389 Activation of Matrix Metalloproteinases
R-RNO-196741 Cobalamin (Cbl, vitamin B12) transport and metabolism
R-RNO-6798695 Neutrophil degranulation
R-RNO-6803157 Antimicrobial peptides

Protein family/group databases

MEROPSiS01.094

Names & Taxonomyi

Protein namesi
Recommended name:
Anionic trypsin-1 (EC:3.4.21.4)
Alternative name(s):
Anionic trypsin I
Pretrypsinogen I
Serine protease 1
Gene namesi
Name:Prss1
Synonyms:Try1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi3417 Prss1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3638328

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15By similarityAdd BLAST15
PropeptideiPRO_000002820716 – 23Activation peptide8
ChainiPRO_000002820824 – 246Anionic trypsin-1Add BLAST223

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 160PROSITE-ProRule annotation
Disulfide bondi48 ↔ 64PROSITE-ProRule annotation
Disulfide bondi132 ↔ 233PROSITE-ProRule annotation
Disulfide bondi139 ↔ 206PROSITE-ProRule annotation
Disulfide bondi171 ↔ 185PROSITE-ProRule annotation
Disulfide bondi196 ↔ 220PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP00762
PRIDEiP00762

PTM databases

iPTMnetiP00762
PhosphoSitePlusiP00762

Expressioni

Gene expression databases

BgeeiENSRNOG00000032156
GenevisibleiP00762 RN

Interactioni

Protein-protein interaction databases

BioGridi246823, 1 interactor
STRINGi10116.ENSRNOP00000043068

Chemistry databases

BindingDBiP00762

Structurei

3D structure databases

ProteinModelPortaliP00762
SMRiP00762
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 244Peptidase S1PROSITE-ProRule annotationAdd BLAST221

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118862
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP00762
KOiK01312
OMAiPEHSVPY
OrthoDBiEOG091G0DF7
PhylomeDBiP00762
TreeFamiTF331065

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00762-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALLILALV GAAVAFPLED DDKIVGGYTC PEHSVPYQVS LNSGYHFCGG
60 70 80 90 100
SLINDQWVVS AAHCYKSRIQ VRLGEHNINV LEGDEQFINA AKIIKHPNYS
110 120 130 140 150
SWTLNNDIML IKLSSPVKLN ARVAPVALPS ACAPAGTQCL ISGWGNTLSN
160 170 180 190 200
GVNNPDLLQC VDAPVLSQAD CEAAYPGEIT SSMICVGFLE GGKDSCQGDS
210 220 230 240
GGPVVCNGQL QGIVSWGYGC ALPDNPGVYT KVCNFVGWIQ DTIAAN
Length:246
Mass (Da):25,959
Last modified:July 21, 1986 - v1
Checksum:i6AFA0DAD11943FB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01273 mRNA Translation: CAA24580.1
J00778 Genomic DNA Translation: AAA98518.1
PIRiB22657 TRRT1
RefSeqiNP_036767.1, NM_012635.1
UniGeneiRn.34435

Genome annotation databases

EnsembliENSRNOT00000040403; ENSRNOP00000043068; ENSRNOG00000032156
GeneIDi24691
KEGGirno:24691
UCSCiRGD:3417 rat

Similar proteinsi

Entry informationi

Entry nameiTRY1_RAT
AccessioniPrimary (citable) accession number: P00762
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 23, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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