Reviewed,
UniProtKB/Swiss-Prot P00762 (TRY1_RAT)
Last modified
January 19, 2010.
Version 101.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Anionic trypsin-1 EC=3.4.21.4 Alternative name(s): Anionic trypsin I Pretrypsinogen I Serine protease 1 | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 246 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. |
| Cofactor | Binds 1 calcium ion per subunit. |
| Subcellular location | |
| Miscellaneous | This sequence represents the precursor of the major form of trypsin produced by the adult pancreas. |
| Sequence similarities | Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Digestion |
| Cellular component | Secreted |
| Domain | Signal |
| Ligand | Calcium Metal-binding |
| Molecular function | Hydrolase Protease Serine protease |
| PTM | Disulfide bond Zymogen |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | digestion Ref.1 Traceable author statement. Source: RGD proteolysisInferred from electronic annotation. Source: InterPro response to caffeineInferred from direct assay. Source: RGD response to nicotineInferred from direct assay. Source: RGD response to nutrientInferred from expression pattern. Source: RGD |
| Cellular component | extracellular space Inferred from direct assay. Source: RGD |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein bindingInferred from direct assay. Source: RGD serine-type endopeptidase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 15 | 15 | By similarity | ||||||||
| Propeptide | 16 – 23 | 8 | Activation peptide | PRO_0000028207 | |||||||
| Chain | 24 – 246 | 223 | Anionic trypsin-1 | PRO_0000028208 | |||||||
Regions | |||||||||||
| Domain | 24 – 244 | 221 | Peptidase S1 | ||||||||
Sites | |||||||||||
| Active site | 63 | 1 | Charge relay system | ||||||||
| Active site | 107 | 1 | Charge relay system | ||||||||
| Active site | 200 | 1 | Charge relay system | ||||||||
| Metal binding | 75 | 1 | Calcium | ||||||||
| Metal binding | 77 | 1 | Calcium; via carbonyl oxygen | ||||||||
| Metal binding | 80 | 1 | Calcium; via carbonyl oxygen | ||||||||
| Metal binding | 85 | 1 | Calcium | ||||||||
| Site | 194 | 1 | Required for specificity | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 30 ↔ 160 | By similarity | |||||||||
| Disulfide bond | 48 ↔ 64 | By similarity | |||||||||
| Disulfide bond | 132 ↔ 233 | By similarity | |||||||||
| Disulfide bond | 139 ↔ 206 | By similarity | |||||||||
| Disulfide bond | 171 ↔ 185 | By similarity | |||||||||
| Disulfide bond | 196 ↔ 220 | By similarity | |||||||||
Sequences
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References
| [1] | "Two similar but nonallelic rat pancreatic trypsinogens. Nucleotide sequences of the cloned cDNAs." MacDonald R.J., Stary S.J., Swift G.H. J. Biol. Chem. 257:9724-9732(1982) [PubMed: 6896710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. |
| [2] | "Structure of two related rat pancreatic trypsin genes." Craik C.S., Choo Q.L., Swift G.H., Quinto C., MacDonald R.J., Rutter W.J. J. Biol. Chem. 259:14255-14264(1984) [PubMed: 6094547] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | Lubec G., Kang S.U., Lubec S., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 73-92 AND 96-112, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Hippocampus. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | V01273 mRNA. Translation: CAA24580.1. J00778 Genomic DNA. Translation: AAA98518.1. |
| IPI | IPI00212767. |
| PIR | TRRT1. B22657. |
| RefSeq | NP_036767.1. |
| UniGene | Rn.34435 |
3D structure databases | |
| SMR | P00762. Positions 24-246. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P00762. |
Protein family/group databases | |
| MEROPS | S01.094. |
Proteomic databases | |
| PRIDE | P00762. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000040403; ENSRNOP00000043068; ENSRNOG00000032156; Rattus norvegicus. [Genome view] |
| GeneID | 24691. |
| KEGG | rno:24691. |
| UCSC | NM_012635. rat. |
Organism-specific databases | |
| CTD | 24691. |
| RGD | 3417. Prss1. |
Phylogenomic databases | |
| eggNOG | roNOG11199. |
| HOVERGEN | P00762. |
| InParanoid | P00762. |
| OMA | GENNIEL. |
| OrthoDB | EOG91K208. |
| PhylomeDB | P00762. |
Enzyme and pathway databases | |
| BRENDA | 3.4.21.4. 248. |
Gene expression databases | |
| ArrayExpress | P00762. |
| Genevestigator | P00762. |
| GermOnline | ENSRNOG00000032156. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view] |
| Pfam | PF00089. Trypsin. 1 hit. [Graphical view] |
| PRINTS | PR00722. CHYMOTRYPSIN. |
| SMART | SM00020. Tryp_SPc. 1 hit. [Graphical view] |
| PROSITE | PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 604133. |
Entry information
| Entry name | TRY1_RAT | ||||||||
| Accession | Primary (citable) accession number: P00762 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


