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Protein

Tonin

Gene

Klk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein has both trypsin- and chymotrypsin-like activities, being able to release angiotensin II from angiotensin I or angiotensinogen.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63Charge relay system1
Metal bindingi63Zinc1
Metal bindingi113Zinc1
Metal bindingi115Zinc1
Active sitei118Charge relay system1
Active sitei211Charge relay system1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiS01.172.

Names & Taxonomyi

Protein namesi
Recommended name:
Tonin (EC:3.4.21.35)
Alternative name(s):
Esterase 1
Glandular kallikrein-2
Short name:
rGK-2
RSKG-5
S2 kallikrein
Gene namesi
Name:Klk2
Synonyms:Klk-2, Ton
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi3888. Ton.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Add BLAST18
PropeptideiPRO_000002800319 – 24Activation peptide4 Publications6
ChainiPRO_000002800425 – 259ToninAdd BLAST235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 171
Disulfide bondi48 ↔ 64
Glycosylationi106N-linked (GlcNAc...)1 Publication1
Disulfide bondi150 ↔ 217
Disulfide bondi182 ↔ 196
Glycosylationi189N-linked (GlcNAc...)1 Publication1
Disulfide bondi207 ↔ 232

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP00759.
PRIDEiP00759.

Expressioni

Tissue specificityi

Found in submaxillary gland.

Gene expression databases

GenevisibleiP00759. RN.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025701.

Structurei

Secondary structure

1259
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi39 – 54Combined sources16
Beta strandi57 – 60Combined sources4
Helixi62 – 64Combined sources3
Beta strandi70 – 74Combined sources5
Beta strandi86 – 88Combined sources3
Beta strandi90 – 95Combined sources6
Beta strandi120 – 126Combined sources7
Beta strandi149 – 156Combined sources8
Beta strandi158 – 162Combined sources5
Beta strandi170 – 177Combined sources8
Helixi179 – 181Combined sources3
Helixi183 – 186Combined sources4
Helixi190 – 193Combined sources4
Beta strandi194 – 198Combined sources5
Beta strandi214 – 217Combined sources4
Beta strandi220 – 225Combined sources6
Beta strandi239 – 243Combined sources5
Helixi244 – 247Combined sources4
Helixi248 – 257Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TONX-ray1.80A25-259[»]
ProteinModelPortaliP00759.
SMRiP00759.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00759.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 256Peptidase S1PROSITE-ProRule annotationAdd BLAST232

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00759.
KOiK01325.
OMAiVINEYLC.
OrthoDBiEOG091G0DF7.
PhylomeDBiP00759.
TreeFamiTF331065.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00759-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLQILSLVL SVGRIDAAPP GQSRIVGGYK CEKNSQPWQV AVINEYLCGG
60 70 80 90 100
VLIDPSWVIT AAHCYSNNYQ VLLGRNNLFK DEPFAQRRLV RQSFRHPDYI
110 120 130 140 150
PLIVTNDTEQ PVHDHSNDLM LLHLSEPADI TGGVKVIDLP TKEPKVGSTC
160 170 180 190 200
LASGWGSTNP SEMVVSHDLQ CVNIHLLSNE KCIETYKDNV TDVMLCAGEM
210 220 230 240 250
EGGKDTCAGD SGGPLICDGV LQGITSGGAT PCAKPKTPAI YAKLIKFTSW

IKKVMKENP
Length:259
Mass (Da):28,248
Last modified:April 1, 1988 - v1
Checksum:i3D6E60D011F926B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11565 mRNA. Translation: AAA41466.1.
M23878, M23877 Genomic DNA. Translation: AAA42259.1.
M26533 Genomic DNA. Translation: AAA42081.1.
PIRiB33359. KQRTTN.
RefSeqiNP_036809.1. NM_012677.1.
UniGeneiRn.9882.

Genome annotation databases

EnsembliENSRNOT00000025701; ENSRNOP00000025701; ENSRNOG00000029237.
GeneIDi24841.
KEGGirno:24841.
UCSCiRGD:3888. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11565 mRNA. Translation: AAA41466.1.
M23878, M23877 Genomic DNA. Translation: AAA42259.1.
M26533 Genomic DNA. Translation: AAA42081.1.
PIRiB33359. KQRTTN.
RefSeqiNP_036809.1. NM_012677.1.
UniGeneiRn.9882.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TONX-ray1.80A25-259[»]
ProteinModelPortaliP00759.
SMRiP00759.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025701.

Protein family/group databases

MEROPSiS01.172.

Proteomic databases

PaxDbiP00759.
PRIDEiP00759.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025701; ENSRNOP00000025701; ENSRNOG00000029237.
GeneIDi24841.
KEGGirno:24841.
UCSCiRGD:3888. rat.

Organism-specific databases

CTDi24841.
RGDi3888. Ton.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00759.
KOiK01325.
OMAiVINEYLC.
OrthoDBiEOG091G0DF7.
PhylomeDBiP00759.
TreeFamiTF331065.

Miscellaneous databases

EvolutionaryTraceiP00759.
PROiP00759.

Gene expression databases

GenevisibleiP00759. RN.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKLK2_RAT
AccessioniPrimary (citable) accession number: P00759
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.