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P00759 (KLK2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tonin

EC=3.4.21.35
Alternative name(s):
Esterase 1
Glandular kallikrein-2
Short name=rGK-2
RSKG-5
S2 kallikrein
Gene names
Name:Klk2
Synonyms:Klk-2, Ton
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein has both trypsin- and chymotrypsin-like activities, being able to release angiotensin II from angiotensin I or angiotensinogen.

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Cofactor

Binds 1 zinc ion per subunit.

Subunit structure

Monomer.

Tissue specificity

Found in submaxillary gland.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818
Propeptide19 – 246Activation peptide
PRO_0000028003
Chain25 – 259235Tonin
PRO_0000028004

Regions

Domain25 – 256232Peptidase S1

Sites

Active site631Charge relay system
Active site1181Charge relay system
Active site2111Charge relay system
Metal binding631Zinc
Metal binding1131Zinc
Metal binding1151Zinc

Amino acid modifications

Glycosylation1061N-linked (GlcNAc...) Ref.4
Glycosylation1891N-linked (GlcNAc...) Ref.4
Disulfide bond31 ↔ 171
Disulfide bond48 ↔ 64
Disulfide bond150 ↔ 217
Disulfide bond182 ↔ 196
Disulfide bond207 ↔ 232

Secondary structure

.................................... 259
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00759 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 3D6E60D011F926B4

FASTA25928,248
        10         20         30         40         50         60 
MWLQILSLVL SVGRIDAAPP GQSRIVGGYK CEKNSQPWQV AVINEYLCGG VLIDPSWVIT 

        70         80         90        100        110        120 
AAHCYSNNYQ VLLGRNNLFK DEPFAQRRLV RQSFRHPDYI PLIVTNDTEQ PVHDHSNDLM 

       130        140        150        160        170        180 
LLHLSEPADI TGGVKVIDLP TKEPKVGSTC LASGWGSTNP SEMVVSHDLQ CVNIHLLSNE 

       190        200        210        220        230        240 
KCIETYKDNV TDVMLCAGEM EGGKDTCAGD SGGPLICDGV LQGITSGGAT PCAKPKTPAI 

       250 
YAKLIKFTSW IKKVMKENP 

« Hide

References

[1]"Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin."
Ashley P.L., MacDonald R.J.
Biochemistry 24:4512-4520(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Organization and expression of the rat kallikrein gene family."
Wines D.R., Brady J.M., Pritchett D.B., Roberts J.L., MacDonald R.J.
J. Biol. Chem. 264:7653-7662(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Characterization of genes encoding rat tonin and a kallikrein-like serine protease."
Shai S.Y., Woodley-Miller C., Chao J., Chao L.
Biochemistry 28:5334-5343(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The complete amino acid sequence of rat submaxillary gland tonin does contain the aspartic acid at the active site: confirmation by protein sequence analysis."
Lazure C., Leduc R., Seidah N.G., Thibault G., Genest J., Chretien M.
Biochem. Cell Biol. 65:321-337(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-259.
[5]"Amino acid sequence of rat submaxillary tonin reveals similarities to serine proteases."
Lazure C., Leduc R., Seidah N.G., Thibault G., Genest J., Chretien M.
Nature 307:555-558(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-103 AND 120-259.
[6]"Observation of tissue prokallikrein activation by some serine proteases, arginine esterases in rat submandibular gland."
Kamada M., Furuhata N., Yamaguchi T., Ikekita M., Kizuki K., Moriya H.
Biochem. Biophys. Res. Commun. 166:231-237(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-34.
[7]"Protein products of the rat kallikrein gene family. Substrate specificities of kallikrein rK2 (tonin) and kallikrein rK9."
Moreau T., Brillard-Bourdet M., Bouhnik J., Gauthier F.
J. Biol. Chem. 267:10045-10051(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-50, CHARACTERIZATION.
[8]"Rat submaxillary gland serine protease, tonin. Structure solution and refinement at 1.8-A resolution."
Fujinaga M., James M.N.G.
J. Mol. Biol. 195:373-396(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M11565 mRNA. Translation: AAA41466.1.
M23878, M23877 Genomic DNA. Translation: AAA42259.1.
M26533 Genomic DNA. Translation: AAA42081.1.
PIRKQRTTN. B33359.
RefSeqNP_036809.1. NM_012677.1.
UniGeneRn.9882.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1TONX-ray1.80A25-259[»]
ProteinModelPortalP00759.
SMRP00759. Positions 25-259.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000025701.

Protein family/group databases

MEROPSS01.172.

Proteomic databases

PaxDbP00759.
PRIDEP00759.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000025701; ENSRNOP00000025701; ENSRNOG00000029237.
GeneID24841.
KEGGrno:24841.
UCSCRGD:3888. rat.

Organism-specific databases

CTD24841.
RGD3888. Ton.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00750000117405.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP00759.
KOK01325.
OMAVINEYLC.
OrthoDBEOG75B84T.
PhylomeDBP00759.
TreeFamTF331065.

Gene expression databases

GenevestigatorP00759.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00759.
NextBio604592.

Entry information

Entry nameKLK2_RAT
AccessionPrimary (citable) accession number: P00759
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references