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P00759

- KLK2_RAT

UniProt

P00759 - KLK2_RAT

Protein

Tonin

Gene

Klk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    This protein has both trypsin- and chymotrypsin-like activities, being able to release angiotensin II from angiotensin I or angiotensinogen.

    Catalytic activityi

    Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

    Cofactori

    Binds 1 zinc ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei63 – 631Charge relay system
    Metal bindingi63 – 631Zinc
    Metal bindingi113 – 1131Zinc
    Metal bindingi115 – 1151Zinc
    Active sitei118 – 1181Charge relay system
    Active sitei211 – 2111Charge relay system

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. serine-type endopeptidase activity Source: InterPro
    3. serine-type peptidase activity Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiS01.172.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tonin (EC:3.4.21.35)
    Alternative name(s):
    Esterase 1
    Glandular kallikrein-2
    Short name:
    rGK-2
    RSKG-5
    S2 kallikrein
    Gene namesi
    Name:Klk2
    Synonyms:Klk-2, Ton
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi3888. Ton.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Add
    BLAST
    Propeptidei19 – 246Activation peptide4 PublicationsPRO_0000028003
    Chaini25 – 259235ToninPRO_0000028004Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 171
    Disulfide bondi48 ↔ 64
    Glycosylationi106 – 1061N-linked (GlcNAc...)1 Publication
    Disulfide bondi150 ↔ 217
    Disulfide bondi182 ↔ 196
    Glycosylationi189 – 1891N-linked (GlcNAc...)1 Publication
    Disulfide bondi207 ↔ 232

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP00759.
    PRIDEiP00759.

    Expressioni

    Tissue specificityi

    Found in submaxillary gland.

    Gene expression databases

    GenevestigatoriP00759.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000025701.

    Structurei

    Secondary structure

    1
    259
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi39 – 5416
    Beta strandi57 – 604
    Helixi62 – 643
    Beta strandi70 – 745
    Beta strandi86 – 883
    Beta strandi90 – 956
    Beta strandi120 – 1267
    Beta strandi149 – 1568
    Beta strandi158 – 1625
    Beta strandi170 – 1778
    Helixi179 – 1813
    Helixi183 – 1864
    Helixi190 – 1934
    Beta strandi194 – 1985
    Beta strandi214 – 2174
    Beta strandi220 – 2256
    Beta strandi239 – 2435
    Helixi244 – 2474
    Helixi248 – 25710

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1TONX-ray1.80A25-259[»]
    ProteinModelPortaliP00759.
    SMRiP00759. Positions 25-259.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00759.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 256232Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00750000117405.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP00759.
    KOiK01325.
    OMAiVINEYLC.
    OrthoDBiEOG75B84T.
    PhylomeDBiP00759.
    TreeFamiTF331065.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00759-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWLQILSLVL SVGRIDAAPP GQSRIVGGYK CEKNSQPWQV AVINEYLCGG    50
    VLIDPSWVIT AAHCYSNNYQ VLLGRNNLFK DEPFAQRRLV RQSFRHPDYI 100
    PLIVTNDTEQ PVHDHSNDLM LLHLSEPADI TGGVKVIDLP TKEPKVGSTC 150
    LASGWGSTNP SEMVVSHDLQ CVNIHLLSNE KCIETYKDNV TDVMLCAGEM 200
    EGGKDTCAGD SGGPLICDGV LQGITSGGAT PCAKPKTPAI YAKLIKFTSW 250
    IKKVMKENP 259
    Length:259
    Mass (Da):28,248
    Last modified:April 1, 1988 - v1
    Checksum:i3D6E60D011F926B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11565 mRNA. Translation: AAA41466.1.
    M23878, M23877 Genomic DNA. Translation: AAA42259.1.
    M26533 Genomic DNA. Translation: AAA42081.1.
    PIRiB33359. KQRTTN.
    RefSeqiNP_036809.1. NM_012677.1.
    UniGeneiRn.9882.

    Genome annotation databases

    EnsembliENSRNOT00000025701; ENSRNOP00000025701; ENSRNOG00000029237.
    GeneIDi24841.
    KEGGirno:24841.
    UCSCiRGD:3888. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11565 mRNA. Translation: AAA41466.1 .
    M23878 , M23877 Genomic DNA. Translation: AAA42259.1 .
    M26533 Genomic DNA. Translation: AAA42081.1 .
    PIRi B33359. KQRTTN.
    RefSeqi NP_036809.1. NM_012677.1.
    UniGenei Rn.9882.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1TON X-ray 1.80 A 25-259 [» ]
    ProteinModelPortali P00759.
    SMRi P00759. Positions 25-259.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000025701.

    Protein family/group databases

    MEROPSi S01.172.

    Proteomic databases

    PaxDbi P00759.
    PRIDEi P00759.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000025701 ; ENSRNOP00000025701 ; ENSRNOG00000029237 .
    GeneIDi 24841.
    KEGGi rno:24841.
    UCSCi RGD:3888. rat.

    Organism-specific databases

    CTDi 24841.
    RGDi 3888. Ton.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00750000117405.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P00759.
    KOi K01325.
    OMAi VINEYLC.
    OrthoDBi EOG75B84T.
    PhylomeDBi P00759.
    TreeFami TF331065.

    Miscellaneous databases

    EvolutionaryTracei P00759.
    NextBioi 604592.

    Gene expression databases

    Genevestigatori P00759.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin."
      Ashley P.L., MacDonald R.J.
      Biochemistry 24:4512-4520(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Organization and expression of the rat kallikrein gene family."
      Wines D.R., Brady J.M., Pritchett D.B., Roberts J.L., MacDonald R.J.
      J. Biol. Chem. 264:7653-7662(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Characterization of genes encoding rat tonin and a kallikrein-like serine protease."
      Shai S.Y., Woodley-Miller C., Chao J., Chao L.
      Biochemistry 28:5334-5343(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The complete amino acid sequence of rat submaxillary gland tonin does contain the aspartic acid at the active site: confirmation by protein sequence analysis."
      Lazure C., Leduc R., Seidah N.G., Thibault G., Genest J., Chretien M.
      Biochem. Cell Biol. 65:321-337(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-259.
    5. "Amino acid sequence of rat submaxillary tonin reveals similarities to serine proteases."
      Lazure C., Leduc R., Seidah N.G., Thibault G., Genest J., Chretien M.
      Nature 307:555-558(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-103 AND 120-259.
    6. "Observation of tissue prokallikrein activation by some serine proteases, arginine esterases in rat submandibular gland."
      Kamada M., Furuhata N., Yamaguchi T., Ikekita M., Kizuki K., Moriya H.
      Biochem. Biophys. Res. Commun. 166:231-237(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-34.
    7. "Protein products of the rat kallikrein gene family. Substrate specificities of kallikrein rK2 (tonin) and kallikrein rK9."
      Moreau T., Brillard-Bourdet M., Bouhnik J., Gauthier F.
      J. Biol. Chem. 267:10045-10051(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-50, CHARACTERIZATION.
    8. "Rat submaxillary gland serine protease, tonin. Structure solution and refinement at 1.8-A resolution."
      Fujinaga M., James M.N.G.
      J. Mol. Biol. 195:373-396(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

    Entry informationi

    Entry nameiKLK2_RAT
    AccessioniPrimary (citable) accession number: P00759
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3