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P00758 (KLK1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kallikrein-1

EC=3.4.21.35
Alternative name(s):
Gamma-NGF
Nerve growth factor gamma chain
PS kallikrein
Pancreatic kallikrein
RGK-1
Short name=rK-1
Tissue kallikrein
True tissue kallikrein
Short name=True kallikrein
Gene names
Name:Ngfg
Synonyms:Klk-1, Klk1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Tissue specificity

High levels in pancreas, submaxillary and parotid glands, spleen, and kidney. Ref.1

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Sequence caution

The sequence AAA41464.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH78784.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA00346.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Probable
Propeptide19 – 246Activation peptide Probable
PRO_0000027999
Chain25 – 261237Kallikrein-1
PRO_0000028000
Chain25 – 11187Nerve growth factor gamma chain 1
PRO_0000028001
Chain112 – 261150Nerve growth factor gamma chain 2
PRO_0000028002

Regions

Domain25 – 258234Peptidase S1

Sites

Active site651Charge relay system
Active site1201Charge relay system
Active site2131Charge relay system

Amino acid modifications

Glycosylation1081N-linked (GlcNAc...) Probable
Disulfide bond31 ↔ 173 By similarity
Disulfide bond50 ↔ 66 By similarity
Disulfide bond152 ↔ 219 By similarity
Disulfide bond184 ↔ 198 By similarity
Disulfide bond209 ↔ 234 By similarity

Sequences

Sequence LengthMass (Da)Tools
P00758 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: F2F99C0227A7882B

FASTA26128,852
        10         20         30         40         50         60 
MWFLILFLAL SLGRNDAAPP VQSRVVGGYN CEMNSQPWQV AVYYFGEYLC GGVLIDPSWV 

        70         80         90        100        110        120 
ITAAHCATDN YQVWLGRNNL YEDEPFAQHR LVSQSFPHPG FNQDLIWNHT RQPGDDYSND 

       130        140        150        160        170        180 
LMLLHLSQPA DITDGVKVID LPIEEPKVGS TCLASGWGSI TPDGLELSDD LQCVNIDLLS 

       190        200        210        220        230        240 
NEKCVEAHKE EVTDLMLCAG EMDGGKDTCK GDSGGPLICN GVLQGITSWG FNPCGEPKKP 

       250        260 
GIYTKLIKFT PWIKEVMKEN P 

« Hide

References

« Hide 'large scale' references
[1]"Rat pancreatic kallikrein mRNA: nucleotide sequence and amino acid sequence of the encoded preproenzyme."
Swift G.H., Dagorn J.-C., Ashley P.L., Cummings S.W., MacDonald R.J.
Proc. Natl. Acad. Sci. U.S.A. 79:7263-7267(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Pancreas.
[2]"Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin."
Ashley P.L., MacDonald R.J.
Biochemistry 24:4512-4520(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Submandibular gland.
[3]"Identification and structure of the rat true tissue kallikrein gene expressed in the kidney."
Inoue H., Fukui K., Miyake Y.
J. Biochem. 105:834-840(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Kidney.
[4]"Organization and expression of the rat kallikrein gene family."
Wines D.R., Brady J.M., Pritchett D.B., Roberts J.L., MacDonald R.J.
J. Biol. Chem. 264:7653-7662(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[6]"Immunological identification of rat tissue kallikrein cDNA and characterization of the kallikrein gene family."
Gerald W.L., Chao J., Chao L.
Biochim. Biophys. Acta 866:1-14(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-261.
Tissue: Submandibular gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J00758 mRNA. No translation available.
M11563 mRNA. Translation: AAA41464.1. Different initiation.
D00448 Genomic DNA. Translation: BAA00346.1. Different initiation.
M23876, M23874, M23875 Genomic DNA. Translation: AAA41462.1.
BC078784 mRNA. Translation: AAH78784.1. Different initiation.
X03560 mRNA. Translation: CAA27247.1.
PIRKQRTP. A00944.
RefSeqNP_113711.1. NM_031523.1.
UniGeneRn.11331.

3D structure databases

ProteinModelPortalP00758.
SMRP00758. Positions 25-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000025831.

Protein family/group databases

MEROPSS01.405.

Proteomic databases

PaxDbP00758.
PRIDEP00758.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000025831; ENSRNOP00000025831; ENSRNOG00000029221.
GeneID24594.
KEGGrno:24594.
UCSCRGD:3175. rat.

Organism-specific databases

CTD3816.
RGD3175. Ngfg.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00750000117405.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP00758.
KOK01325.
OrthoDBEOG75B84T.
PhylomeDBP00758.
TreeFamTF331065.

Enzyme and pathway databases

BRENDA3.4.21.35. 5301.

Gene expression databases

GenevestigatorP00758.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio603788.

Entry information

Entry nameKLK1_RAT
AccessionPrimary (citable) accession number: P00758
Secondary accession number(s): Q68G17
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries