P00758 (KLK1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kallikrein-1
EC=3.4.21.35

Alternative name(s):
Gamma-NGF
Nerve growth factor gamma chain
PS kallikrein
Pancreatic kallikrein
RGK-1
Short name=rK-1
Tissue kallikrein
True tissue kallikrein
Short name=True kallikrein

Cleaved into the following 2 chains:

1. Nerve growth factor gamma chain 1
2. Nerve growth factor gamma chain 2

Gene names

Name:	Ngfg
Synonyms:	Klk-1, Klk1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Tissue specificity	High levels in pancreas, submaxillary and parotid glands, spleen, and kidney. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.
Sequence caution	The sequence AAA41464.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH78784.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA00346.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 24	6	Activation peptide Probable		PRO_0000027999
	Chain	25 – 261	237	Kallikrein-1		PRO_0000028000
	Chain	25 – 111	87	Nerve growth factor gamma chain 1		PRO_0000028001
	Chain	112 – 261	150	Nerve growth factor gamma chain 2		PRO_0000028002
Regions
	Domain	25 – 258	234	Peptidase S1
Sites
	Active site	65	1	Charge relay system
	Active site	120	1	Charge relay system
	Active site	213	1	Charge relay system
Amino acid modifications
	Glycosylation	108	1	N-linked (GlcNAc...) Probable
	Disulfide bond	31 ↔ 173		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	152 ↔ 219		By similarity
	Disulfide bond	184 ↔ 198		By similarity
	Disulfide bond	209 ↔ 234		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00758 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: F2F99C0227A7882B	FASTA	261	28,852
	10 20 30 40 50 60 MWFLILFLAL SLGRNDAAPP VQSRVVGGYN CEMNSQPWQV AVYYFGEYLC GGVLIDPSWV 70 80 90 100 110 120 ITAAHCATDN YQVWLGRNNL YEDEPFAQHR LVSQSFPHPG FNQDLIWNHT RQPGDDYSND 130 140 150 160 170 180 LMLLHLSQPA DITDGVKVID LPIEEPKVGS TCLASGWGSI TPDGLELSDD LQCVNIDLLS 190 200 210 220 230 240 NEKCVEAHKE EVTDLMLCAG EMDGGKDTCK GDSGGPLICN GVLQGITSWG FNPCGEPKKP 250 260 GIYTKLIKFT PWIKEVMKEN P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat pancreatic kallikrein mRNA: nucleotide sequence and amino acid sequence of the encoded preproenzyme." Swift G.H., Dagorn J.-C., Ashley P.L., Cummings S.W., MacDonald R.J. Proc. Natl. Acad. Sci. U.S.A. 79:7263-7267(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Pancreas.
[2]	"Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin." Ashley P.L., MacDonald R.J. Biochemistry 24:4512-4520(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Submandibular gland.
[3]	"Identification and structure of the rat true tissue kallikrein gene expressed in the kidney." Inoue H., Fukui K., Miyake Y. J. Biochem. 105:834-840(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Kidney.
[4]	"Organization and expression of the rat kallikrein gene family." Wines D.R., Brady J.M., Pritchett D.B., Roberts J.L., MacDonald R.J. J. Biol. Chem. 264:7653-7662(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[6]	"Immunological identification of rat tissue kallikrein cDNA and characterization of the kallikrein gene family." Gerald W.L., Chao J., Chao L. Biochim. Biophys. Acta 866:1-14(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-261. Tissue: Submandibular gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J00758 mRNA. No translation available. M11563 mRNA. Translation: AAA41464.1. Different initiation. D00448 Genomic DNA. Translation: BAA00346.1. Different initiation. M23876, M23874, M23875 Genomic DNA. Translation: AAA41462.1. BC078784 mRNA. Translation: AAH78784.1. Different initiation. X03560 mRNA. Translation: CAA27247.1.
IPI	IPI00231193.
PIR	KQRTP. A00944.
RefSeq	NP_113711.1. NM_031523.1.
UniGene	Rn.11331.
3D structure databases
ProteinModelPortal	P00758.
SMR	P00758. Positions 25-261.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000025831.
Protein family/group databases
MEROPS	S01.405.
Proteomic databases
PaxDb	P00758.
PRIDE	P00758.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025831; ENSRNOP00000025831; ENSRNOG00000029221.
GeneID	24594.
KEGG	rno:24594.
UCSC	RGD:3175. rat.
Organism-specific databases
CTD	3816.
RGD	3175. Ngfg.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00690000101721.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P00758.
KO	K01325.
OrthoDB	EOG4DZ1VW.
Enzyme and pathway databases
BRENDA	3.4.21.35. 5301.
Gene expression databases
Genevestigator	P00758.
GermOnline	ENSRNOG00000029221. Rattus norvegicus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603788.

Entry information

Entry name

KLK1_RAT

Accession

Primary (citable) accession number: P00758
Secondary accession number(s): Q68G17

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program