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P00758

- KLK1_RAT

UniProt

P00758 - KLK1_RAT

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Protein
Kallikrein-1
Gene
Ngfg, Klk-1, Klk1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei120 – 1201Charge relay system
Active sitei213 – 2131Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    BRENDAi3.4.21.35. 5301.

    Protein family/group databases

    MEROPSiS01.405.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kallikrein-1 (EC:3.4.21.35)
    Alternative name(s):
    Gamma-NGF
    Nerve growth factor gamma chain
    PS kallikrein
    Pancreatic kallikrein
    RGK-1
    Short name:
    rK-1
    Tissue kallikrein
    True tissue kallikrein
    Short name:
    True kallikrein
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Ngfg
    Synonyms:Klk-1, Klk1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi3175. Ngfg.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818 Inferred
    Add
    BLAST
    Propeptidei19 – 246Activation peptide Inferred
    PRO_0000027999
    Chaini25 – 261237Kallikrein-1
    PRO_0000028000Add
    BLAST
    Chaini25 – 11187Nerve growth factor gamma chain 1
    PRO_0000028001Add
    BLAST
    Chaini112 – 261150Nerve growth factor gamma chain 2
    PRO_0000028002Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 173 By similarity
    Disulfide bondi50 ↔ 66 By similarity
    Glycosylationi108 – 1081N-linked (GlcNAc...) Inferred
    Disulfide bondi152 ↔ 219 By similarity
    Disulfide bondi184 ↔ 198 By similarity
    Disulfide bondi209 ↔ 234 By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP00758.
    PRIDEiP00758.

    Expressioni

    Tissue specificityi

    High levels in pancreas, submaxillary and parotid glands, spleen, and kidney.1 Publication

    Gene expression databases

    GenevestigatoriP00758.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000025831.

    Structurei

    3D structure databases

    ProteinModelPortaliP00758.
    SMRiP00758. Positions 25-261.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 258234Peptidase S1
    Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00750000117405.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP00758.
    KOiK01325.
    OrthoDBiEOG75B84T.
    PhylomeDBiP00758.
    TreeFamiTF331065.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00758-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWFLILFLAL SLGRNDAAPP VQSRVVGGYN CEMNSQPWQV AVYYFGEYLC    50
    GGVLIDPSWV ITAAHCATDN YQVWLGRNNL YEDEPFAQHR LVSQSFPHPG 100
    FNQDLIWNHT RQPGDDYSND LMLLHLSQPA DITDGVKVID LPIEEPKVGS 150
    TCLASGWGSI TPDGLELSDD LQCVNIDLLS NEKCVEAHKE EVTDLMLCAG 200
    EMDGGKDTCK GDSGGPLICN GVLQGITSWG FNPCGEPKKP GIYTKLIKFT 250
    PWIKEVMKEN P 261
    Length:261
    Mass (Da):28,852
    Last modified:June 1, 1994 - v2
    Checksum:iF2F99C0227A7882B
    GO

    Sequence cautioni

    The sequence AAA41464.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAH78784.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAA00346.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00758 mRNA. No translation available.
    M11563 mRNA. Translation: AAA41464.1. Different initiation.
    D00448 Genomic DNA. Translation: BAA00346.1. Different initiation.
    M23876, M23874, M23875 Genomic DNA. Translation: AAA41462.1.
    BC078784 mRNA. Translation: AAH78784.1. Different initiation.
    X03560 mRNA. Translation: CAA27247.1.
    PIRiA00944. KQRTP.
    RefSeqiNP_113711.1. NM_031523.1.
    UniGeneiRn.11331.

    Genome annotation databases

    EnsembliENSRNOT00000025831; ENSRNOP00000025831; ENSRNOG00000029221.
    GeneIDi24594.
    KEGGirno:24594.
    UCSCiRGD:3175. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00758 mRNA. No translation available.
    M11563 mRNA. Translation: AAA41464.1 . Different initiation.
    D00448 Genomic DNA. Translation: BAA00346.1 . Different initiation.
    M23876 , M23874 , M23875 Genomic DNA. Translation: AAA41462.1 .
    BC078784 mRNA. Translation: AAH78784.1 . Different initiation.
    X03560 mRNA. Translation: CAA27247.1 .
    PIRi A00944. KQRTP.
    RefSeqi NP_113711.1. NM_031523.1.
    UniGenei Rn.11331.

    3D structure databases

    ProteinModelPortali P00758.
    SMRi P00758. Positions 25-261.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000025831.

    Protein family/group databases

    MEROPSi S01.405.

    Proteomic databases

    PaxDbi P00758.
    PRIDEi P00758.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000025831 ; ENSRNOP00000025831 ; ENSRNOG00000029221 .
    GeneIDi 24594.
    KEGGi rno:24594.
    UCSCi RGD:3175. rat.

    Organism-specific databases

    CTDi 3816.
    RGDi 3175. Ngfg.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00750000117405.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P00758.
    KOi K01325.
    OrthoDBi EOG75B84T.
    PhylomeDBi P00758.
    TreeFami TF331065.

    Enzyme and pathway databases

    BRENDAi 3.4.21.35. 5301.

    Miscellaneous databases

    NextBioi 603788.

    Gene expression databases

    Genevestigatori P00758.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Rat pancreatic kallikrein mRNA: nucleotide sequence and amino acid sequence of the encoded preproenzyme."
      Swift G.H., Dagorn J.-C., Ashley P.L., Cummings S.W., MacDonald R.J.
      Proc. Natl. Acad. Sci. U.S.A. 79:7263-7267(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Pancreas.
    2. "Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin."
      Ashley P.L., MacDonald R.J.
      Biochemistry 24:4512-4520(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Submandibular gland.
    3. "Identification and structure of the rat true tissue kallikrein gene expressed in the kidney."
      Inoue H., Fukui K., Miyake Y.
      J. Biochem. 105:834-840(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Kidney.
    4. "Organization and expression of the rat kallikrein gene family."
      Wines D.R., Brady J.M., Pritchett D.B., Roberts J.L., MacDonald R.J.
      J. Biol. Chem. 264:7653-7662(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    6. "Immunological identification of rat tissue kallikrein cDNA and characterization of the kallikrein gene family."
      Gerald W.L., Chao J., Chao L.
      Biochim. Biophys. Acta 866:1-14(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-261.
      Tissue: Submandibular gland.

    Entry informationi

    Entry nameiKLK1_RAT
    AccessioniPrimary (citable) accession number: P00758
    Secondary accession number(s): Q68G17
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: June 1, 1994
    Last modified: May 14, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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