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P00758

- KLK1_RAT

UniProt

P00758 - KLK1_RAT

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Protein

Kallikrein-1

Gene
Ngfg, Klk-1, Klk1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei120 – 1201Charge relay system
Active sitei213 – 2131Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.35. 5301.

Protein family/group databases

MEROPSiS01.405.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein-1 (EC:3.4.21.35)
Alternative name(s):
Gamma-NGF
Nerve growth factor gamma chain
PS kallikrein
Pancreatic kallikrein
RGK-1
Short name:
rK-1
Tissue kallikrein
True tissue kallikrein
Short name:
True kallikrein
Cleaved into the following 2 chains:
Gene namesi
Name:Ngfg
Synonyms:Klk-1, Klk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi3175. Ngfg.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 InferredAdd
BLAST
Propeptidei19 – 246Activation peptide InferredPRO_0000027999
Chaini25 – 261237Kallikrein-1PRO_0000028000Add
BLAST
Chaini25 – 11187Nerve growth factor gamma chain 1PRO_0000028001Add
BLAST
Chaini112 – 261150Nerve growth factor gamma chain 2PRO_0000028002Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 173 By similarity
Disulfide bondi50 ↔ 66 By similarity
Glycosylationi108 – 1081N-linked (GlcNAc...) Inferred
Disulfide bondi152 ↔ 219 By similarity
Disulfide bondi184 ↔ 198 By similarity
Disulfide bondi209 ↔ 234 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP00758.
PRIDEiP00758.

Expressioni

Tissue specificityi

High levels in pancreas, submaxillary and parotid glands, spleen, and kidney.1 Publication

Gene expression databases

GenevestigatoriP00758.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025831.

Structurei

3D structure databases

ProteinModelPortaliP00758.
SMRiP00758. Positions 25-261.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 258234Peptidase S1Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00750000117405.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00758.
KOiK01325.
OrthoDBiEOG75B84T.
PhylomeDBiP00758.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00758-1 [UniParc]FASTAAdd to Basket

« Hide

MWFLILFLAL SLGRNDAAPP VQSRVVGGYN CEMNSQPWQV AVYYFGEYLC    50
GGVLIDPSWV ITAAHCATDN YQVWLGRNNL YEDEPFAQHR LVSQSFPHPG 100
FNQDLIWNHT RQPGDDYSND LMLLHLSQPA DITDGVKVID LPIEEPKVGS 150
TCLASGWGSI TPDGLELSDD LQCVNIDLLS NEKCVEAHKE EVTDLMLCAG 200
EMDGGKDTCK GDSGGPLICN GVLQGITSWG FNPCGEPKKP GIYTKLIKFT 250
PWIKEVMKEN P 261
Length:261
Mass (Da):28,852
Last modified:June 1, 1994 - v2
Checksum:iF2F99C0227A7882B
GO

Sequence cautioni

The sequence AAA41464.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH78784.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAA00346.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J00758 mRNA. No translation available.
M11563 mRNA. Translation: AAA41464.1. Different initiation.
D00448 Genomic DNA. Translation: BAA00346.1. Different initiation.
M23876, M23874, M23875 Genomic DNA. Translation: AAA41462.1.
BC078784 mRNA. Translation: AAH78784.1. Different initiation.
X03560 mRNA. Translation: CAA27247.1.
PIRiA00944. KQRTP.
RefSeqiNP_113711.1. NM_031523.1.
UniGeneiRn.11331.

Genome annotation databases

EnsembliENSRNOT00000025831; ENSRNOP00000025831; ENSRNOG00000029221.
GeneIDi24594.
KEGGirno:24594.
UCSCiRGD:3175. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J00758 mRNA. No translation available.
M11563 mRNA. Translation: AAA41464.1 . Different initiation.
D00448 Genomic DNA. Translation: BAA00346.1 . Different initiation.
M23876 , M23874 , M23875 Genomic DNA. Translation: AAA41462.1 .
BC078784 mRNA. Translation: AAH78784.1 . Different initiation.
X03560 mRNA. Translation: CAA27247.1 .
PIRi A00944. KQRTP.
RefSeqi NP_113711.1. NM_031523.1.
UniGenei Rn.11331.

3D structure databases

ProteinModelPortali P00758.
SMRi P00758. Positions 25-261.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000025831.

Protein family/group databases

MEROPSi S01.405.

Proteomic databases

PaxDbi P00758.
PRIDEi P00758.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000025831 ; ENSRNOP00000025831 ; ENSRNOG00000029221 .
GeneIDi 24594.
KEGGi rno:24594.
UCSCi RGD:3175. rat.

Organism-specific databases

CTDi 3816.
RGDi 3175. Ngfg.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00750000117405.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi P00758.
KOi K01325.
OrthoDBi EOG75B84T.
PhylomeDBi P00758.
TreeFami TF331065.

Enzyme and pathway databases

BRENDAi 3.4.21.35. 5301.

Miscellaneous databases

NextBioi 603788.

Gene expression databases

Genevestigatori P00758.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rat pancreatic kallikrein mRNA: nucleotide sequence and amino acid sequence of the encoded preproenzyme."
    Swift G.H., Dagorn J.-C., Ashley P.L., Cummings S.W., MacDonald R.J.
    Proc. Natl. Acad. Sci. U.S.A. 79:7263-7267(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Pancreas.
  2. "Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin."
    Ashley P.L., MacDonald R.J.
    Biochemistry 24:4512-4520(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Submandibular gland.
  3. "Identification and structure of the rat true tissue kallikrein gene expressed in the kidney."
    Inoue H., Fukui K., Miyake Y.
    J. Biochem. 105:834-840(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Kidney.
  4. "Organization and expression of the rat kallikrein gene family."
    Wines D.R., Brady J.M., Pritchett D.B., Roberts J.L., MacDonald R.J.
    J. Biol. Chem. 264:7653-7662(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  6. "Immunological identification of rat tissue kallikrein cDNA and characterization of the kallikrein gene family."
    Gerald W.L., Chao J., Chao L.
    Biochim. Biophys. Acta 866:1-14(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-261.
    Tissue: Submandibular gland.

Entry informationi

Entry nameiKLK1_RAT
AccessioniPrimary (citable) accession number: P00758
Secondary accession number(s): Q68G17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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