Reviewed, UniProtKB/Swiss-Prot P00758 (KLK1_RAT)

Names and origin

Protein names

Recommended name:
    Kallikrein-1
    EC=3.4.21.35
Alternative name(s):
    Gamma-NGF
    Tissue kallikrein
    True tissue kallikrein
      Short name=True kallikrein
    PS kallikrein
    RGK-1
      Short name=rK-1
    Pancreatic kallikrein
    Nerve growth factor gamma chain
Cleaved into the following 2 chains:
    1- Recommended name:
            Nerve growth factor gamma chain 1
    2- Recommended name:
            Nerve growth factor gamma chain 2

Gene names

Name:	Ngfg
Synonyms:	Klk-1, Klk1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Tissue specificity	High levels in pancreas, submaxillary and parotid glands, spleen, and kidney. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 24	6	Activation peptide Probable		PRO_0000027999
	Chain	25 – 261	237	Kallikrein-1		PRO_0000028000
	Chain	25 – 111	87	Nerve growth factor gamma chain 1		PRO_0000028001
	Chain	112 – 261	150	Nerve growth factor gamma chain 2		PRO_0000028002
Regions
	Domain	25 – 258	234	Peptidase S1
Sites
	Active site	65	1	Charge relay system
	Active site	120	1	Charge relay system
	Active site	213	1	Charge relay system
Amino acid modifications
	Glycosylation	108	1	N-linked (GlcNAc...) Probable
	Disulfide bond	31 ↔ 173		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	152 ↔ 219		By similarity
	Disulfide bond	184 ↔ 198		By similarity
	Disulfide bond	209 ↔ 234		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00758-1 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: F2F99C0227A7882B	FASTA	261	28,852
	10 20 30 40 50 60 MWFLILFLAL SLGRNDAAPP VQSRVVGGYN CEMNSQPWQV AVYYFGEYLC GGVLIDPSWV 70 80 90 100 110 120 ITAAHCATDN YQVWLGRNNL YEDEPFAQHR LVSQSFPHPG FNQDLIWNHT RQPGDDYSND 130 140 150 160 170 180 LMLLHLSQPA DITDGVKVID LPIEEPKVGS TCLASGWGSI TPDGLELSDD LQCVNIDLLS 190 200 210 220 230 240 NEKCVEAHKE EVTDLMLCAG EMDGGKDTCK GDSGGPLICN GVLQGITSWG FNPCGEPKKP 250 260 GIYTKLIKFT PWIKEVMKEN P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat pancreatic kallikrein mRNA: nucleotide sequence and amino acid sequence of the encoded preproenzyme." Swift G.H., Dagorn J.-C., Ashley P.L., Cummings S.W., MacDonald R.J. Proc. Natl. Acad. Sci. U.S.A. 79:7263-7267(1982) [PubMed: 6961406] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Pancreas.
[2]	"Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin." Ashley P.L., MacDonald R.J. Biochemistry 24:4512-4520(1985) [PubMed: 2998455] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Submandibular gland.
[3]	"Identification and structure of the rat true tissue kallikrein gene expressed in the kidney." Inoue H., Fukui K., Miyake Y. J. Biochem. 105:834-840(1989) [PubMed: 2753879] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Kidney.
[4]	"Organization and expression of the rat kallikrein gene family." Wines D.R., Brady J.M., Pritchett D.B., Roberts J.L., MacDonald R.J. J. Biol. Chem. 264:7653-7662(1989) [PubMed: 2708383] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[6]	"Immunological identification of rat tissue kallikrein cDNA and characterization of the kallikrein gene family." Gerald W.L., Chao J., Chao L. Biochim. Biophys. Acta 866:1-14(1986) [PubMed: 3004582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-261. Tissue: Submandibular gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J00758 mRNA. No translation available. M11563 mRNA. Translation: AAA41464.1. Different initiation. D00448 Genomic DNA. Translation: BAA00346.1. Different initiation. M23876, M23874, M23875 Genomic DNA. Translation: AAA41462.1. BC078784 mRNA. Translation: AAH78784.1. Different initiation. X03560 mRNA. Translation: CAA27247.1.
IPI	IPI00231193.
PIR	KQRTP. A00944.
RefSeq	NP_113711.1.
UniGene	Rn.11331
3D structure databases
HSSP	HSSP built from PDB template 1SGF based on UniProtKB P00757.
SMR	P00758. Positions 25-261.
ModBase	Search...
Protein family/group databases
MEROPS	S01.405.
Genome annotation databases
Ensembl	ENSRNOG00000029221. Rattus norvegicus. [Contig view]
GeneID	24594.
KEGG	rno:24594.
Organism-specific databases
RGD	3175. Ngfg.
Phylogenomic databases
HOVERGEN	P00758.
Enzyme and pathway databases
BRENDA	3.4.21.35. 248.
Gene expression databases
ArrayExpress	P00758.
GermOnline	ENSRNOG00000029221. Rattus norvegicus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	603788.

Entry information

Entry name

KLK1_RAT

Accession

Primary (citable) accession number: P00758
Secondary accession number(s): Q68G17

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 1, 1994
Last modified:	May 5, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)