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Protein

Kallikrein-1

Gene

Ngfg

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei120 – 1201Charge relay system
Active sitei213 – 2131Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.35. 5301.

Protein family/group databases

MEROPSiS01.405.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein-1 (EC:3.4.21.35)
Alternative name(s):
Gamma-NGF
Nerve growth factor gamma chain
PS kallikrein
Pancreatic kallikrein
RGK-1
Short name:
rK-1
Tissue kallikrein
True tissue kallikrein
Short name:
True kallikrein
Cleaved into the following 2 chains:
Gene namesi
Name:Ngfg
Synonyms:Klk-1, Klk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi3175. Ngfg.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818CuratedAdd
BLAST
Propeptidei19 – 246Activation peptideCuratedPRO_0000027999
Chaini25 – 261237Kallikrein-1PRO_0000028000Add
BLAST
Chaini25 – 11187Nerve growth factor gamma chain 1PRO_0000028001Add
BLAST
Chaini112 – 261150Nerve growth factor gamma chain 2PRO_0000028002Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 173PROSITE-ProRule annotation
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Glycosylationi108 – 1081N-linked (GlcNAc...)Curated
Disulfide bondi152 ↔ 219PROSITE-ProRule annotation
Disulfide bondi184 ↔ 198PROSITE-ProRule annotation
Disulfide bondi209 ↔ 234PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP00758.
PRIDEiP00758.

Expressioni

Tissue specificityi

High levels in pancreas, submaxillary and parotid glands, spleen, and kidney.1 Publication

Gene expression databases

GenevestigatoriP00758.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025831.

Structurei

3D structure databases

ProteinModelPortaliP00758.
SMRiP00758. Positions 25-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 258234Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00758.
KOiK01325.
OrthoDBiEOG75B84T.
PhylomeDBiP00758.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00758-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFLILFLAL SLGRNDAAPP VQSRVVGGYN CEMNSQPWQV AVYYFGEYLC
60 70 80 90 100
GGVLIDPSWV ITAAHCATDN YQVWLGRNNL YEDEPFAQHR LVSQSFPHPG
110 120 130 140 150
FNQDLIWNHT RQPGDDYSND LMLLHLSQPA DITDGVKVID LPIEEPKVGS
160 170 180 190 200
TCLASGWGSI TPDGLELSDD LQCVNIDLLS NEKCVEAHKE EVTDLMLCAG
210 220 230 240 250
EMDGGKDTCK GDSGGPLICN GVLQGITSWG FNPCGEPKKP GIYTKLIKFT
260
PWIKEVMKEN P
Length:261
Mass (Da):28,852
Last modified:May 31, 1994 - v2
Checksum:iF2F99C0227A7882B
GO

Sequence cautioni

The sequence AAA41464.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH78784.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAA00346.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00758 mRNA. No translation available.
M11563 mRNA. Translation: AAA41464.1. Different initiation.
D00448 Genomic DNA. Translation: BAA00346.1. Different initiation.
M23876, M23874, M23875 Genomic DNA. Translation: AAA41462.1.
BC078784 mRNA. Translation: AAH78784.1. Different initiation.
X03560 mRNA. Translation: CAA27247.1.
PIRiA00944. KQRTP.
RefSeqiNP_113711.1. NM_031523.1.
UniGeneiRn.11331.

Genome annotation databases

GeneIDi24594.
KEGGirno:24594.
UCSCiRGD:3175. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00758 mRNA. No translation available.
M11563 mRNA. Translation: AAA41464.1. Different initiation.
D00448 Genomic DNA. Translation: BAA00346.1. Different initiation.
M23876, M23874, M23875 Genomic DNA. Translation: AAA41462.1.
BC078784 mRNA. Translation: AAH78784.1. Different initiation.
X03560 mRNA. Translation: CAA27247.1.
PIRiA00944. KQRTP.
RefSeqiNP_113711.1. NM_031523.1.
UniGeneiRn.11331.

3D structure databases

ProteinModelPortaliP00758.
SMRiP00758. Positions 25-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025831.

Protein family/group databases

MEROPSiS01.405.

Proteomic databases

PaxDbiP00758.
PRIDEiP00758.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24594.
KEGGirno:24594.
UCSCiRGD:3175. rat.

Organism-specific databases

CTDi3816.
RGDi3175. Ngfg.

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00758.
KOiK01325.
OrthoDBiEOG75B84T.
PhylomeDBiP00758.
TreeFamiTF331065.

Enzyme and pathway databases

BRENDAi3.4.21.35. 5301.

Miscellaneous databases

NextBioi603788.

Gene expression databases

GenevestigatoriP00758.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rat pancreatic kallikrein mRNA: nucleotide sequence and amino acid sequence of the encoded preproenzyme."
    Swift G.H., Dagorn J.-C., Ashley P.L., Cummings S.W., MacDonald R.J.
    Proc. Natl. Acad. Sci. U.S.A. 79:7263-7267(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Pancreas.
  2. "Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin."
    Ashley P.L., MacDonald R.J.
    Biochemistry 24:4512-4520(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Submandibular gland.
  3. "Identification and structure of the rat true tissue kallikrein gene expressed in the kidney."
    Inoue H., Fukui K., Miyake Y.
    J. Biochem. 105:834-840(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Kidney.
  4. "Organization and expression of the rat kallikrein gene family."
    Wines D.R., Brady J.M., Pritchett D.B., Roberts J.L., MacDonald R.J.
    J. Biol. Chem. 264:7653-7662(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  6. "Immunological identification of rat tissue kallikrein cDNA and characterization of the kallikrein gene family."
    Gerald W.L., Chao J., Chao L.
    Biochim. Biophys. Acta 866:1-14(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-261.
    Tissue: Submandibular gland.

Entry informationi

Entry nameiKLK1_RAT
AccessioniPrimary (citable) accession number: P00758
Secondary accession number(s): Q68G17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 20, 1986
Last sequence update: May 31, 1994
Last modified: March 3, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.