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Protein

Kallikrein 1-related peptidase-like b4

Gene

Klk1b4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per 7S complex. The Zn2+ ions are bound at the alpha-gamma interfaces.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771Zinc
Metal bindingi84 – 841Zinc

GO - Molecular functioni

GO - Biological processi

  • positive regulation of phosphatidylinositol 3-kinase activity Source: MGI
  • small GTPase mediated signal transduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Serine protease homolog

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Protein family/group databases

MEROPSiS01.931.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase-like b4
Alternative name(s):
7S nerve growth factor alpha chain
Alpha-NGF
Gene namesi
Name:Klk1b4
Synonyms:Klk-4, Klk4, Ngfa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97320. Klk1b4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17171 PublicationAdd
BLAST
Chaini18 – 256239Kallikrein 1-related peptidase-like b4PRO_0000027972Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi45 ↔ 61PROSITE-ProRule annotation
Disulfide bondi147 ↔ 214PROSITE-ProRule annotation
Disulfide bondi179 ↔ 193PROSITE-ProRule annotation
Disulfide bondi204 ↔ 229PROSITE-ProRule annotation

Post-translational modificationi

The presence of Gln-24 prevents cleavage of the activation peptide, which remains attached at the amino end of the mature alpha chain.

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP00757.
PaxDbiP00757.
PeptideAtlasiP00757.
PRIDEiP00757.

Expressioni

Gene expression databases

BgeeiENSMUSG00000066513.
CleanExiMM_KLK1B4.
GenevisibleiP00757. MM.

Interactioni

Subunit structurei

7S nerve growth factor is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076576.

Structurei

Secondary structure

1
256
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi34 – 385Combined sources
Beta strandi45 – 495Combined sources
Beta strandi51 – 577Combined sources
Helixi59 – 613Combined sources
Beta strandi67 – 704Combined sources
Beta strandi83 – 9210Combined sources
Helixi98 – 1003Combined sources
Beta strandi117 – 1237Combined sources
Beta strandi146 – 1494Combined sources
Beta strandi169 – 1746Combined sources
Helixi177 – 1804Combined sources
Beta strandi184 – 1863Combined sources
Beta strandi191 – 1966Combined sources
Beta strandi198 – 2036Combined sources
Beta strandi211 – 2144Combined sources
Beta strandi217 – 2226Combined sources
Beta strandi236 – 2416Combined sources
Helixi242 – 2443Combined sources
Helixi245 – 2539Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SGFX-ray3.15A/X17-256[»]
ProteinModelPortaliP00757.
SMRiP00757. Positions 30-254.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00757.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 253236Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni18 – 247Activation peptide homolog

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00757.
KOiK05451.
OMAiSSWIRET.
OrthoDBiEOG091G0DF7.
PhylomeDBiP00757.
TreeFamiTF331065.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00757-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFLILFLAL SLGGIDAAPP VQSQVDCENS QPWHVAVYRF NKYQCGGVLL
60 70 80 90 100
DRNWVLTAAH CYNDKYQVWL GKNNFLEDEP SDQHRLVSKA IPHPDFNMSL
110 120 130 140 150
LNEHTPQPED DYSNDLMLLR LSKPADITDV VKPITLPTEE PKLGSTCLAS
160 170 180 190 200
GWGSTTPIKF KYPDDLQCVN LKLLPNEDCD KAHEMKVTDA MLCAGEMDGG
210 220 230 240 250
SYTCEHDSGG PLICDGILQG ITSWGPEPCG EPTEPSVYTK LIKFSSWIRE

TMANNP
Length:256
Mass (Da):28,548
Last modified:January 1, 1988 - v1
Checksum:i9F6690E21982C3DC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441Q → L AA sequence (PubMed:6712944).Curated
Sequence conflicti140 – 1401E → C AA sequence (PubMed:6712944).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti184 – 1841E → K.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01800 Genomic DNA. Translation: CAA25931.1.
X01801 Genomic DNA. Translation: CAA25932.1.
M11434 mRNA. Translation: AAA39819.1.
BC034518 mRNA. Translation: AAH34518.1.
CCDSiCCDS21200.1.
PIRiB91005. NGMSA.
RefSeqiNP_035045.2. NM_010915.4.
UniGeneiMm.440886.

Genome annotation databases

EnsembliENSMUST00000077354; ENSMUSP00000076576; ENSMUSG00000066513.
GeneIDi18048.
KEGGimmu:18048.
UCSCiuc009gom.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01800 Genomic DNA. Translation: CAA25931.1.
X01801 Genomic DNA. Translation: CAA25932.1.
M11434 mRNA. Translation: AAA39819.1.
BC034518 mRNA. Translation: AAH34518.1.
CCDSiCCDS21200.1.
PIRiB91005. NGMSA.
RefSeqiNP_035045.2. NM_010915.4.
UniGeneiMm.440886.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SGFX-ray3.15A/X17-256[»]
ProteinModelPortaliP00757.
SMRiP00757. Positions 30-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076576.

Protein family/group databases

MEROPSiS01.931.

Proteomic databases

MaxQBiP00757.
PaxDbiP00757.
PeptideAtlasiP00757.
PRIDEiP00757.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077354; ENSMUSP00000076576; ENSMUSG00000066513.
GeneIDi18048.
KEGGimmu:18048.
UCSCiuc009gom.2. mouse.

Organism-specific databases

CTDi18048.
MGIiMGI:97320. Klk1b4.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00757.
KOiK05451.
OMAiSSWIRET.
OrthoDBiEOG091G0DF7.
PhylomeDBiP00757.
TreeFamiTF331065.

Enzyme and pathway databases

ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

EvolutionaryTraceiP00757.
PROiP00757.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000066513.
CleanExiMM_KLK1B4.
GenevisibleiP00757. MM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiK1KB4_MOUSE
AccessioniPrimary (citable) accession number: P00757
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: September 7, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Has no demonstrable enzymatic activity. This may be due to several critical changes in its sequence, relative to those of related proteases.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.