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Protein

Kallikrein 1-related peptidase-like b4

Gene

Klk1b4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Caution

Has no demonstrable enzymatic activity. This may be due to several critical changes in its sequence, relative to those of related proteases.Curated

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per 7S complex. The Zn2+ ions are bound at the alpha-gamma interfaces.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi77Zinc1
Metal bindingi84Zinc1

GO - Molecular functioni

GO - Biological processi

  • positive regulation of phosphatidylinositol 3-kinase activity Source: MGI
  • small GTPase mediated signal transduction Source: MGI

Keywordsi

Molecular functionGrowth factor, Serine protease homolog
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiS01.931

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase-like b4
Alternative name(s):
7S nerve growth factor alpha chain
Alpha-NGF
Gene namesi
Name:Klk1b4
Synonyms:Klk-4, Klk4, Ngfa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97320 Klk1b4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 171 PublicationAdd BLAST17
ChainiPRO_000002797218 – 256Kallikrein 1-related peptidase-like b4Add BLAST239

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi45 ↔ 61PROSITE-ProRule annotation
Disulfide bondi147 ↔ 214PROSITE-ProRule annotation
Disulfide bondi179 ↔ 193PROSITE-ProRule annotation
Disulfide bondi204 ↔ 229PROSITE-ProRule annotation

Post-translational modificationi

The presence of Gln-24 prevents cleavage of the activation peptide, which remains attached at the amino end of the mature alpha chain.

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP00757
PaxDbiP00757
PeptideAtlasiP00757
PRIDEiP00757

Expressioni

Gene expression databases

BgeeiENSMUSG00000066513
CleanExiMM_KLK1B4
ExpressionAtlasiP00757 baseline and differential
GenevisibleiP00757 MM

Interactioni

Subunit structurei

7S nerve growth factor is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076576

Structurei

Secondary structure

1256
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 38Combined sources5
Beta strandi45 – 49Combined sources5
Beta strandi51 – 57Combined sources7
Helixi59 – 61Combined sources3
Beta strandi67 – 70Combined sources4
Beta strandi83 – 92Combined sources10
Helixi98 – 100Combined sources3
Beta strandi117 – 123Combined sources7
Beta strandi146 – 149Combined sources4
Beta strandi169 – 174Combined sources6
Helixi177 – 180Combined sources4
Beta strandi184 – 186Combined sources3
Beta strandi191 – 196Combined sources6
Beta strandi198 – 203Combined sources6
Beta strandi211 – 214Combined sources4
Beta strandi217 – 222Combined sources6
Beta strandi236 – 241Combined sources6
Helixi242 – 244Combined sources3
Helixi245 – 253Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SGFX-ray3.15A/X17-256[»]
ProteinModelPortaliP00757
SMRiP00757
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00757

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 253Peptidase S1PROSITE-ProRule annotationAdd BLAST236

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni18 – 24Activation peptide homolog7

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00910000144067
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP00757
KOiK05451
OMAiSSWIRET
OrthoDBiEOG091G0DF7
PhylomeDBiP00757
TreeFamiTF331065

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00757-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFLILFLAL SLGGIDAAPP VQSQVDCENS QPWHVAVYRF NKYQCGGVLL
60 70 80 90 100
DRNWVLTAAH CYNDKYQVWL GKNNFLEDEP SDQHRLVSKA IPHPDFNMSL
110 120 130 140 150
LNEHTPQPED DYSNDLMLLR LSKPADITDV VKPITLPTEE PKLGSTCLAS
160 170 180 190 200
GWGSTTPIKF KYPDDLQCVN LKLLPNEDCD KAHEMKVTDA MLCAGEMDGG
210 220 230 240 250
SYTCEHDSGG PLICDGILQG ITSWGPEPCG EPTEPSVYTK LIKFSSWIRE

TMANNP
Length:256
Mass (Da):28,548
Last modified:January 1, 1988 - v1
Checksum:i9F6690E21982C3DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44Q → L AA sequence (PubMed:6712944).Curated1
Sequence conflicti140E → C AA sequence (PubMed:6712944).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti184E → K. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01800 Genomic DNA Translation: CAA25931.1
X01801 Genomic DNA Translation: CAA25932.1
M11434 mRNA Translation: AAA39819.1
BC034518 mRNA Translation: AAH34518.1
CCDSiCCDS21200.1
PIRiB91005 NGMSA
RefSeqiNP_035045.2, NM_010915.4
UniGeneiMm.440886

Genome annotation databases

EnsembliENSMUST00000077354; ENSMUSP00000076576; ENSMUSG00000066513
GeneIDi18048
KEGGimmu:18048
UCSCiuc009gom.2 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiK1KB4_MOUSE
AccessioniPrimary (citable) accession number: P00757
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: April 25, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health