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Protein

Kallikrein 1-related peptidase-like b4

Gene

Klk1b4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per 7S complex. The Zn2+ ions are bound at the alpha-gamma interfaces.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi77Zinc1
Metal bindingi84Zinc1

GO - Molecular functioni

GO - Biological processi

  • positive regulation of phosphatidylinositol 3-kinase activity Source: MGI
  • small GTPase mediated signal transduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Serine protease homolog

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Protein family/group databases

MEROPSiS01.931.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase-like b4
Alternative name(s):
7S nerve growth factor alpha chain
Alpha-NGF
Gene namesi
Name:Klk1b4
Synonyms:Klk-4, Klk4, Ngfa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97320. Klk1b4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 171 PublicationAdd BLAST17
ChainiPRO_000002797218 – 256Kallikrein 1-related peptidase-like b4Add BLAST239

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi45 ↔ 61PROSITE-ProRule annotation
Disulfide bondi147 ↔ 214PROSITE-ProRule annotation
Disulfide bondi179 ↔ 193PROSITE-ProRule annotation
Disulfide bondi204 ↔ 229PROSITE-ProRule annotation

Post-translational modificationi

The presence of Gln-24 prevents cleavage of the activation peptide, which remains attached at the amino end of the mature alpha chain.

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP00757.
PaxDbiP00757.
PeptideAtlasiP00757.
PRIDEiP00757.

Expressioni

Gene expression databases

BgeeiENSMUSG00000066513.
CleanExiMM_KLK1B4.
GenevisibleiP00757. MM.

Interactioni

Subunit structurei

7S nerve growth factor is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076576.

Structurei

Secondary structure

1256
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 38Combined sources5
Beta strandi45 – 49Combined sources5
Beta strandi51 – 57Combined sources7
Helixi59 – 61Combined sources3
Beta strandi67 – 70Combined sources4
Beta strandi83 – 92Combined sources10
Helixi98 – 100Combined sources3
Beta strandi117 – 123Combined sources7
Beta strandi146 – 149Combined sources4
Beta strandi169 – 174Combined sources6
Helixi177 – 180Combined sources4
Beta strandi184 – 186Combined sources3
Beta strandi191 – 196Combined sources6
Beta strandi198 – 203Combined sources6
Beta strandi211 – 214Combined sources4
Beta strandi217 – 222Combined sources6
Beta strandi236 – 241Combined sources6
Helixi242 – 244Combined sources3
Helixi245 – 253Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SGFX-ray3.15A/X17-256[»]
ProteinModelPortaliP00757.
SMRiP00757.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00757.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 253Peptidase S1PROSITE-ProRule annotationAdd BLAST236

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni18 – 24Activation peptide homolog7

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00757.
KOiK05451.
OMAiSSWIRET.
OrthoDBiEOG091G0DF7.
PhylomeDBiP00757.
TreeFamiTF331065.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00757-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFLILFLAL SLGGIDAAPP VQSQVDCENS QPWHVAVYRF NKYQCGGVLL
60 70 80 90 100
DRNWVLTAAH CYNDKYQVWL GKNNFLEDEP SDQHRLVSKA IPHPDFNMSL
110 120 130 140 150
LNEHTPQPED DYSNDLMLLR LSKPADITDV VKPITLPTEE PKLGSTCLAS
160 170 180 190 200
GWGSTTPIKF KYPDDLQCVN LKLLPNEDCD KAHEMKVTDA MLCAGEMDGG
210 220 230 240 250
SYTCEHDSGG PLICDGILQG ITSWGPEPCG EPTEPSVYTK LIKFSSWIRE

TMANNP
Length:256
Mass (Da):28,548
Last modified:January 1, 1988 - v1
Checksum:i9F6690E21982C3DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44Q → L AA sequence (PubMed:6712944).Curated1
Sequence conflicti140E → C AA sequence (PubMed:6712944).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti184E → K.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01800 Genomic DNA. Translation: CAA25931.1.
X01801 Genomic DNA. Translation: CAA25932.1.
M11434 mRNA. Translation: AAA39819.1.
BC034518 mRNA. Translation: AAH34518.1.
CCDSiCCDS21200.1.
PIRiB91005. NGMSA.
RefSeqiNP_035045.2. NM_010915.4.
UniGeneiMm.440886.

Genome annotation databases

EnsembliENSMUST00000077354; ENSMUSP00000076576; ENSMUSG00000066513.
GeneIDi18048.
KEGGimmu:18048.
UCSCiuc009gom.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01800 Genomic DNA. Translation: CAA25931.1.
X01801 Genomic DNA. Translation: CAA25932.1.
M11434 mRNA. Translation: AAA39819.1.
BC034518 mRNA. Translation: AAH34518.1.
CCDSiCCDS21200.1.
PIRiB91005. NGMSA.
RefSeqiNP_035045.2. NM_010915.4.
UniGeneiMm.440886.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SGFX-ray3.15A/X17-256[»]
ProteinModelPortaliP00757.
SMRiP00757.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076576.

Protein family/group databases

MEROPSiS01.931.

Proteomic databases

MaxQBiP00757.
PaxDbiP00757.
PeptideAtlasiP00757.
PRIDEiP00757.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077354; ENSMUSP00000076576; ENSMUSG00000066513.
GeneIDi18048.
KEGGimmu:18048.
UCSCiuc009gom.2. mouse.

Organism-specific databases

CTDi18048.
MGIiMGI:97320. Klk1b4.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00757.
KOiK05451.
OMAiSSWIRET.
OrthoDBiEOG091G0DF7.
PhylomeDBiP00757.
TreeFamiTF331065.

Enzyme and pathway databases

ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

EvolutionaryTraceiP00757.
PROiP00757.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000066513.
CleanExiMM_KLK1B4.
GenevisibleiP00757. MM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiK1KB4_MOUSE
AccessioniPrimary (citable) accession number: P00757
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: November 30, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Has no demonstrable enzymatic activity. This may be due to several critical changes in its sequence, relative to those of related proteases.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.