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Protein

Kallikrein 1-related peptidase b3

Gene

Klk1b3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

7S NGF alpha chain stabilizes the 7S complex. The beta dimer promotes neurite growth. The gamma chain is an arginine-specific protease; it may also have plasminogen activator activity, as well as mitogenic activity for chick embryo fibroblasts.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Cofactori

Zn2+Note: Binds 2 Zn(2+) ions per 7S complex. The Zn2+ ions are bound at the alpha-gamma interfaces.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei120 – 1201Charge relay system
Active sitei213 – 2131Charge relay system
Metal bindingi231 – 2311Zinc
Metal bindingi236 – 2361Zinc

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. receptor signaling protein activity Source: MGI
  3. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. intracellular signal transduction Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Hydrolase, Protease, Serine protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.21.77. 3474.
ReactomeiREACT_331346. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
REACT_332323. Activation of Matrix Metalloproteinases.

Protein family/group databases

MEROPSiS01.170.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b3 (EC:3.4.21.35)
Alternative name(s):
7S nerve growth factor gamma chain
Gamma-NGF
Glandular kallikrein K3
Short name:
mGK-3
Tissue kallikrein-3
Cleaved into the following 2 chains:
Gene namesi
Name:Klk1b3
Synonyms:Klk-3, Klk3, Ngfg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97322. Klk1b3.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818CuratedAdd
BLAST
Propeptidei19 – 246Activation peptide1 PublicationPRO_0000027968
Chaini25 – 261237Kallikrein 1-related peptidase b3PRO_0000027969Add
BLAST
Chaini25 – 10783Nerve growth factor gamma chain 1PRO_0000027970Add
BLAST
Chaini112 – 261150Nerve growth factor gamma chain 2PRO_0000027971Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 173
Disulfide bondi50 ↔ 66
Glycosylationi102 – 1021N-linked (GlcNAc...)1 Publication
Disulfide bondi152 ↔ 219
Disulfide bondi184 ↔ 198
Disulfide bondi209 ↔ 234

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP00756.
PaxDbiP00756.
PRIDEiP00756.

Expressioni

Gene expression databases

BgeeiP00756.
CleanExiMM_KLK1B3.
ExpressionAtlasiP00756. baseline and differential.
GenevestigatoriP00756.

Interactioni

Subunit structurei

7S nerve growth factor is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains.

Structurei

Secondary structure

1
261
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi33 – 353Combined sources
Beta strandi39 – 446Combined sources
Beta strandi47 – 5610Combined sources
Beta strandi59 – 624Combined sources
Helixi64 – 663Combined sources
Beta strandi72 – 765Combined sources
Beta strandi88 – 9710Combined sources
Helixi103 – 1053Combined sources
Beta strandi122 – 1287Combined sources
Beta strandi133 – 1353Combined sources
Beta strandi151 – 16414Combined sources
Beta strandi172 – 1798Combined sources
Helixi181 – 1877Combined sources
Beta strandi196 – 2005Combined sources
Beta strandi202 – 2054Combined sources
Beta strandi216 – 2194Combined sources
Beta strandi222 – 2298Combined sources
Beta strandi241 – 2455Combined sources
Helixi246 – 2494Combined sources
Helixi250 – 2589Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SGFX-ray3.15G/Z25-261[»]
ProteinModelPortaliP00756.
SMRiP00756. Positions 25-261.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00756.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 258234Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 10783Segment B1Add
BLAST
Regioni112 – 261150Segment AAdd
BLAST
Regioni112 – 16453Segment CAdd
BLAST
Regioni165 – 26197Segment B2Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00756.
KOiK01325.
OMAiCAGHIEG.
OrthoDBiEOG75B84T.
PhylomeDBiP00756.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00756-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFLILFLAL SLGGIDAAPP VQSRIVGGFK CEKNSQPWHV AVYRYTQYLC
60 70 80 90 100
GGVLLDPNWV LTAAHCYDDN YKVWLGKNNL FKDEPSAQHR FVSKAIPHPG
110 120 130 140 150
FNMSLMRKHI RFLEYDYSND LMLLRLSKPA DITDTVKPIT LPTEEPKLGS
160 170 180 190 200
TCLASGWGSI TPTKFQFTDD LYCVNLKLLP NEDCAKAHIE KVTDAMLCAG
210 220 230 240 250
EMDGGKDTCK GDSGGPLICD GVLQGITSWG HTPCGEPDMP GVYTKLNKFT
260
SWIKDTMAKN P
Length:261
Mass (Da):28,998
Last modified:July 20, 1986 - v1
Checksum:i4870748E174AF7C8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1114Missing (PubMed:3848399).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01389 mRNA. Translation: CAA25645.1.
X01798 Genomic DNA. Translation: CAA25928.1.
X01799 Genomic DNA. Translation: CAA25930.1.
BC132657 mRNA. Translation: AAI32658.1.
BC132659 mRNA. Translation: AAI32660.1.
CCDSiCCDS21199.1.
PIRiA91005. NGMSG.
RefSeqiNP_032719.1. NM_008693.2.
UniGeneiMm.439740.

Genome annotation databases

EnsembliENSMUST00000085450; ENSMUSP00000082577; ENSMUSG00000066515.
GeneIDi18050.
KEGGimmu:18050.
UCSCiuc009gol.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01389 mRNA. Translation: CAA25645.1.
X01798 Genomic DNA. Translation: CAA25928.1.
X01799 Genomic DNA. Translation: CAA25930.1.
BC132657 mRNA. Translation: AAI32658.1.
BC132659 mRNA. Translation: AAI32660.1.
CCDSiCCDS21199.1.
PIRiA91005. NGMSG.
RefSeqiNP_032719.1. NM_008693.2.
UniGeneiMm.439740.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SGFX-ray3.15G/Z25-261[»]
ProteinModelPortaliP00756.
SMRiP00756. Positions 25-261.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.170.

Proteomic databases

MaxQBiP00756.
PaxDbiP00756.
PRIDEiP00756.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000085450; ENSMUSP00000082577; ENSMUSG00000066515.
GeneIDi18050.
KEGGimmu:18050.
UCSCiuc009gol.2. mouse.

Organism-specific databases

CTDi18050.
MGIiMGI:97322. Klk1b3.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00756.
KOiK01325.
OMAiCAGHIEG.
OrthoDBiEOG75B84T.
PhylomeDBiP00756.
TreeFamiTF331065.

Enzyme and pathway databases

BRENDAi3.4.21.77. 3474.
ReactomeiREACT_331346. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
REACT_332323. Activation of Matrix Metalloproteinases.

Miscellaneous databases

EvolutionaryTraceiP00756.
NextBioi293179.
PROiP00756.
SOURCEiSearch...

Gene expression databases

BgeeiP00756.
CleanExiMM_KLK1B3.
ExpressionAtlasiP00756. baseline and differential.
GenevestigatoriP00756.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a cDNA clone coding for the gamma-subunit of mouse nerve growth factor using a high-stringency selection procedure."
    Ullrich A., Gray A., Wood W.I., Hayflick J., Seeburg P.H.
    DNA 3:387-392(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genes for the alpha and gamma subunits of mouse nerve growth factor are contiguous."
    Evans B.A., Richards R.I.
    EMBO J. 4:133-138(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "The amino acid sequence of the gamma-subunit of mouse submaxillary gland 7 S nerve growth factor."
    Thomas K.A., Baglan N.C., Bradshaw R.A.
    J. Biol. Chem. 256:9156-9166(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-261.
    Tissue: Submandibular gland.
  5. "Structure of mouse 7S NGF: a complex of nerve growth factor with four binding proteins."
    Bax B., Blundell T.L., Murray-Rust J., McDonald N.Q.
    Structure 5:1275-1285(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) OF 7S COMPLEX.
    Strain: Swiss Webster.
    Tissue: Submandibular gland.

Entry informationi

Entry nameiK1KB3_MOUSE
AccessioniPrimary (citable) accession number: P00756
Secondary accession number(s): A2RTW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 20, 1986
Last sequence update: July 20, 1986
Last modified: March 31, 2015
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This precursor is cleaved into segments to produce the active form of the gamma chain, which occurs naturally as combinations of either two or three segments held together by disulfide bonds: B1 and A, or B1, C and B2.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.