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P00756 (K1KB3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kallikrein 1-related peptidase b3

EC=3.4.21.35
Alternative name(s):
7S nerve growth factor gamma chain
Gamma-NGF
Glandular kallikrein K3
Short name=mGK-3
Tissue kallikrein-3
Gene names
Name:Klk1b3
Synonyms:Klk-3, Klk3, Ngfg
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

7S NGF alpha chain stabilizes the 7S complex. The beta dimer promotes neurite growth. The gamma chain is an arginine-specific protease; it may also have plasminogen activator activity, as well as mitogenic activity for chick embryo fibroblasts.

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Cofactor

Binds 2 zinc ions per 7S complex. The zinc ions are bound at the alpha-gamma interfaces.

Subunit structure

7S nerve growth factor is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains.

Miscellaneous

This precursor is cleaved into segments to produce the active form of the gamma chain, which occurs naturally as combinations of either two or three segments held together by disulfide bonds: B1 and A, or B1, C and B2.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Probable
Propeptide19 – 246Activation peptide
PRO_0000027968
Chain25 – 261237Kallikrein 1-related peptidase b3
PRO_0000027969
Chain25 – 10783Nerve growth factor gamma chain 1
PRO_0000027970
Chain112 – 261150Nerve growth factor gamma chain 2
PRO_0000027971

Regions

Domain25 – 258234Peptidase S1
Region25 – 10783Segment B1
Region112 – 261150Segment A
Region112 – 16453Segment C
Region165 – 26197Segment B2

Sites

Active site651Charge relay system
Active site1201Charge relay system
Active site2131Charge relay system
Metal binding2311Zinc
Metal binding2361Zinc

Amino acid modifications

Glycosylation1021N-linked (GlcNAc...) Ref.4
Disulfide bond31 ↔ 173
Disulfide bond50 ↔ 66
Disulfide bond152 ↔ 219
Disulfide bond184 ↔ 198
Disulfide bond209 ↔ 234

Experimental info

Sequence conflict108 – 1114Missing Ref.2

Secondary structure

....................................... 261
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00756 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 4870748E174AF7C8

FASTA26128,998
        10         20         30         40         50         60 
MWFLILFLAL SLGGIDAAPP VQSRIVGGFK CEKNSQPWHV AVYRYTQYLC GGVLLDPNWV 

        70         80         90        100        110        120 
LTAAHCYDDN YKVWLGKNNL FKDEPSAQHR FVSKAIPHPG FNMSLMRKHI RFLEYDYSND 

       130        140        150        160        170        180 
LMLLRLSKPA DITDTVKPIT LPTEEPKLGS TCLASGWGSI TPTKFQFTDD LYCVNLKLLP 

       190        200        210        220        230        240 
NEDCAKAHIE KVTDAMLCAG EMDGGKDTCK GDSGGPLICD GVLQGITSWG HTPCGEPDMP 

       250        260 
GVYTKLNKFT SWIKDTMAKN P 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of a cDNA clone coding for the gamma-subunit of mouse nerve growth factor using a high-stringency selection procedure."
Ullrich A., Gray A., Wood W.I., Hayflick J., Seeburg P.H.
DNA 3:387-392(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Genes for the alpha and gamma subunits of mouse nerve growth factor are contiguous."
Evans B.A., Richards R.I.
EMBO J. 4:133-138(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"The amino acid sequence of the gamma-subunit of mouse submaxillary gland 7 S nerve growth factor."
Thomas K.A., Baglan N.C., Bradshaw R.A.
J. Biol. Chem. 256:9156-9166(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-261.
Tissue: Submandibular gland.
[5]"Structure of mouse 7S NGF: a complex of nerve growth factor with four binding proteins."
Bax B., Blundell T.L., Murray-Rust J., McDonald N.Q.
Structure 5:1275-1285(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) OF 7S COMPLEX.
Strain: Swiss Webster.
Tissue: Submandibular gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X01389 mRNA. Translation: CAA25645.1.
X01798 Genomic DNA. Translation: CAA25928.1.
X01799 Genomic DNA. Translation: CAA25930.1.
BC132657 mRNA. Translation: AAI32658.1.
BC132659 mRNA. Translation: AAI32660.1.
CCDSCCDS21199.1.
PIRNGMSG. A91005.
RefSeqNP_032719.1. NM_008693.2.
UniGeneMm.439740.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1SGFX-ray3.15G/Z25-261[»]
ProteinModelPortalP00756.
SMRP00756. Positions 25-261.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.170.

Proteomic databases

MaxQBP00756.
PaxDbP00756.
PRIDEP00756.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000085450; ENSMUSP00000082577; ENSMUSG00000066515.
GeneID18050.
KEGGmmu:18050.
UCSCuc009gol.2. mouse.

Organism-specific databases

CTD18050.
MGIMGI:97322. Klk1b3.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00750000117405.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidA2RTW1.
KOK01325.
OMANDECDKA.
OrthoDBEOG75B84T.
PhylomeDBP00756.
TreeFamTF331065.

Enzyme and pathway databases

BRENDA3.4.21.77. 3474.

Gene expression databases

ArrayExpressP00756.
BgeeP00756.
CleanExMM_KLK1B3.
GenevestigatorP00756.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00756.
NextBio293179.
PROP00756.
SOURCESearch...

Entry information

Entry nameK1KB3_MOUSE
AccessionPrimary (citable) accession number: P00756
Secondary accession number(s): A2RTW1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot