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Protein

Kallikrein 1-related peptidase b3

Gene

Klk1b3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

7S NGF alpha chain stabilizes the 7S complex. The beta dimer promotes neurite growth. The gamma chain is an arginine-specific protease; it may also have plasminogen activator activity, as well as mitogenic activity for chick embryo fibroblasts.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per 7S complex. The Zn2+ ions are bound at the alpha-gamma interfaces.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay system1
Active sitei120Charge relay system1
Active sitei213Charge relay system1
Metal bindingi231Zinc1
Metal bindingi236Zinc1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Hydrolase, Protease, Serine protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.21.77. 3474.
ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Protein family/group databases

MEROPSiS01.170.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b3 (EC:3.4.21.35)
Alternative name(s):
7S nerve growth factor gamma chain
Gamma-NGF
Glandular kallikrein K3
Short name:
mGK-3
Tissue kallikrein-3
Cleaved into the following 2 chains:
Gene namesi
Name:Klk1b3
Synonyms:Klk-3, Klk3, Ngfg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97322. Klk1b3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18CuratedAdd BLAST18
PropeptideiPRO_000002796819 – 24Activation peptide1 Publication6
ChainiPRO_000002796925 – 261Kallikrein 1-related peptidase b3Add BLAST237
ChainiPRO_000002797025 – 107Nerve growth factor gamma chain 1Add BLAST83
ChainiPRO_0000027971112 – 261Nerve growth factor gamma chain 2Add BLAST150

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 173
Disulfide bondi50 ↔ 66
Glycosylationi102N-linked (GlcNAc...)1 Publication1
Disulfide bondi152 ↔ 219
Disulfide bondi184 ↔ 198
Disulfide bondi209 ↔ 234

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP00756.
PaxDbiP00756.
PeptideAtlasiP00756.
PRIDEiP00756.

Expressioni

Gene expression databases

BgeeiENSMUSG00000066515.
CleanExiMM_KLK1B3.
GenevisibleiP00756. MM.

Interactioni

Subunit structurei

7S nerve growth factor is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000082577.

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi33 – 35Combined sources3
Beta strandi39 – 44Combined sources6
Beta strandi47 – 56Combined sources10
Beta strandi59 – 62Combined sources4
Helixi64 – 66Combined sources3
Beta strandi72 – 76Combined sources5
Beta strandi88 – 97Combined sources10
Helixi103 – 105Combined sources3
Beta strandi122 – 128Combined sources7
Beta strandi133 – 135Combined sources3
Beta strandi151 – 164Combined sources14
Beta strandi172 – 179Combined sources8
Helixi181 – 187Combined sources7
Beta strandi196 – 200Combined sources5
Beta strandi202 – 205Combined sources4
Beta strandi216 – 219Combined sources4
Beta strandi222 – 229Combined sources8
Beta strandi241 – 245Combined sources5
Helixi246 – 249Combined sources4
Helixi250 – 258Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SGFX-ray3.15G/Z25-261[»]
ProteinModelPortaliP00756.
SMRiP00756.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00756.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 258Peptidase S1PROSITE-ProRule annotationAdd BLAST234

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 107Segment B1Add BLAST83
Regioni112 – 261Segment AAdd BLAST150
Regioni112 – 164Segment CAdd BLAST53
Regioni165 – 261Segment B2Add BLAST97

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00756.
KOiK01325.
OMAiYNCEMNS.
OrthoDBiEOG091G0DF7.
PhylomeDBiP00756.
TreeFamiTF331065.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00756-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFLILFLAL SLGGIDAAPP VQSRIVGGFK CEKNSQPWHV AVYRYTQYLC
60 70 80 90 100
GGVLLDPNWV LTAAHCYDDN YKVWLGKNNL FKDEPSAQHR FVSKAIPHPG
110 120 130 140 150
FNMSLMRKHI RFLEYDYSND LMLLRLSKPA DITDTVKPIT LPTEEPKLGS
160 170 180 190 200
TCLASGWGSI TPTKFQFTDD LYCVNLKLLP NEDCAKAHIE KVTDAMLCAG
210 220 230 240 250
EMDGGKDTCK GDSGGPLICD GVLQGITSWG HTPCGEPDMP GVYTKLNKFT
260
SWIKDTMAKN P
Length:261
Mass (Da):28,998
Last modified:July 21, 1986 - v1
Checksum:i4870748E174AF7C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti108 – 111Missing (PubMed:3848399).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01389 mRNA. Translation: CAA25645.1.
X01798 Genomic DNA. Translation: CAA25928.1.
X01799 Genomic DNA. Translation: CAA25930.1.
BC132657 mRNA. Translation: AAI32658.1.
BC132659 mRNA. Translation: AAI32660.1.
CCDSiCCDS21199.1.
PIRiA91005. NGMSG.
RefSeqiNP_032719.1. NM_008693.2.
UniGeneiMm.439740.

Genome annotation databases

EnsembliENSMUST00000085450; ENSMUSP00000082577; ENSMUSG00000066515.
GeneIDi18050.
KEGGimmu:18050.
UCSCiuc009gol.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01389 mRNA. Translation: CAA25645.1.
X01798 Genomic DNA. Translation: CAA25928.1.
X01799 Genomic DNA. Translation: CAA25930.1.
BC132657 mRNA. Translation: AAI32658.1.
BC132659 mRNA. Translation: AAI32660.1.
CCDSiCCDS21199.1.
PIRiA91005. NGMSG.
RefSeqiNP_032719.1. NM_008693.2.
UniGeneiMm.439740.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SGFX-ray3.15G/Z25-261[»]
ProteinModelPortaliP00756.
SMRiP00756.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000082577.

Protein family/group databases

MEROPSiS01.170.

Proteomic databases

MaxQBiP00756.
PaxDbiP00756.
PeptideAtlasiP00756.
PRIDEiP00756.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000085450; ENSMUSP00000082577; ENSMUSG00000066515.
GeneIDi18050.
KEGGimmu:18050.
UCSCiuc009gol.2. mouse.

Organism-specific databases

CTDi18050.
MGIiMGI:97322. Klk1b3.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP00756.
KOiK01325.
OMAiYNCEMNS.
OrthoDBiEOG091G0DF7.
PhylomeDBiP00756.
TreeFamiTF331065.

Enzyme and pathway databases

BRENDAi3.4.21.77. 3474.
ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

EvolutionaryTraceiP00756.
PROiP00756.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000066515.
CleanExiMM_KLK1B3.
GenevisibleiP00756. MM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiK1KB3_MOUSE
AccessioniPrimary (citable) accession number: P00756
Secondary accession number(s): A2RTW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 161 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This precursor is cleaved into segments to produce the active form of the gamma chain, which occurs naturally as combinations of either two or three segments held together by disulfide bonds: B1 and A, or B1, C and B2.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.