SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P00756

- K1KB3_MOUSE

UniProt

P00756 - K1KB3_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Kallikrein 1-related peptidase b3
Gene
Klk1b3, Klk-3, Klk3, Ngfg
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

7S NGF alpha chain stabilizes the 7S complex. The beta dimer promotes neurite growth. The gamma chain is an arginine-specific protease; it may also have plasminogen activator activity, as well as mitogenic activity for chick embryo fibroblasts.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Cofactori

Binds 2 zinc ions per 7S complex. The zinc ions are bound at the alpha-gamma interfaces.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei120 – 1201Charge relay system
Active sitei213 – 2131Charge relay system
Metal bindingi231 – 2311Zinc
Metal bindingi236 – 2361Zinc

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. receptor signaling protein activity Source: MGI
  3. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. intracellular signal transduction Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Hydrolase, Protease, Serine protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.21.77. 3474.
ReactomeiREACT_199000. Activation of Matrix Metalloproteinases.

Protein family/group databases

MEROPSiS01.170.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b3 (EC:3.4.21.35)
Alternative name(s):
7S nerve growth factor gamma chain
Gamma-NGF
Glandular kallikrein K3
Short name:
mGK-3
Tissue kallikrein-3
Cleaved into the following 2 chains:
Gene namesi
Name:Klk1b3
Synonyms:Klk-3, Klk3, Ngfg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:97322. Klk1b3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Inferred
Add
BLAST
Propeptidei19 – 246Activation peptide
PRO_0000027968
Chaini25 – 261237Kallikrein 1-related peptidase b3
PRO_0000027969Add
BLAST
Chaini25 – 10783Nerve growth factor gamma chain 1
PRO_0000027970Add
BLAST
Chaini112 – 261150Nerve growth factor gamma chain 2
PRO_0000027971Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 173
Disulfide bondi50 ↔ 66
Glycosylationi102 – 1021N-linked (GlcNAc...)1 Publication
Disulfide bondi152 ↔ 219
Disulfide bondi184 ↔ 198
Disulfide bondi209 ↔ 234

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP00756.
PaxDbiP00756.
PRIDEiP00756.

Expressioni

Gene expression databases

ArrayExpressiP00756.
BgeeiP00756.
CleanExiMM_KLK1B3.
GenevestigatoriP00756.

Interactioni

Subunit structurei

7S nerve growth factor is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi33 – 353
Beta strandi39 – 446
Beta strandi47 – 5610
Beta strandi59 – 624
Helixi64 – 663
Beta strandi72 – 765
Beta strandi88 – 9710
Helixi103 – 1053
Beta strandi122 – 1287
Beta strandi133 – 1353
Beta strandi151 – 16414
Beta strandi172 – 1798
Helixi181 – 1877
Beta strandi196 – 2005
Beta strandi202 – 2054
Beta strandi216 – 2194
Beta strandi222 – 2298
Beta strandi241 – 2455
Helixi246 – 2494
Helixi250 – 2589

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SGFX-ray3.15G/Z25-261[»]
ProteinModelPortaliP00756.
SMRiP00756. Positions 25-261.

Miscellaneous databases

EvolutionaryTraceiP00756.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 258234Peptidase S1
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 10783Segment B1
Add
BLAST
Regioni112 – 261150Segment A
Add
BLAST
Regioni112 – 16453Segment C
Add
BLAST
Regioni165 – 26197Segment B2
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00750000117405.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiA2RTW1.
KOiK01325.
OMAiNDECDKA.
OrthoDBiEOG75B84T.
PhylomeDBiP00756.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00756-1 [UniParc]FASTAAdd to Basket

« Hide

MWFLILFLAL SLGGIDAAPP VQSRIVGGFK CEKNSQPWHV AVYRYTQYLC    50
GGVLLDPNWV LTAAHCYDDN YKVWLGKNNL FKDEPSAQHR FVSKAIPHPG 100
FNMSLMRKHI RFLEYDYSND LMLLRLSKPA DITDTVKPIT LPTEEPKLGS 150
TCLASGWGSI TPTKFQFTDD LYCVNLKLLP NEDCAKAHIE KVTDAMLCAG 200
EMDGGKDTCK GDSGGPLICD GVLQGITSWG HTPCGEPDMP GVYTKLNKFT 250
SWIKDTMAKN P 261
Length:261
Mass (Da):28,998
Last modified:July 21, 1986 - v1
Checksum:i4870748E174AF7C8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1114Missing1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01389 mRNA. Translation: CAA25645.1.
X01798 Genomic DNA. Translation: CAA25928.1.
X01799 Genomic DNA. Translation: CAA25930.1.
BC132657 mRNA. Translation: AAI32658.1.
BC132659 mRNA. Translation: AAI32660.1.
CCDSiCCDS21199.1.
PIRiA91005. NGMSG.
RefSeqiNP_032719.1. NM_008693.2.
UniGeneiMm.439740.

Genome annotation databases

EnsembliENSMUST00000085450; ENSMUSP00000082577; ENSMUSG00000066515.
GeneIDi18050.
KEGGimmu:18050.
UCSCiuc009gol.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01389 mRNA. Translation: CAA25645.1 .
X01798 Genomic DNA. Translation: CAA25928.1 .
X01799 Genomic DNA. Translation: CAA25930.1 .
BC132657 mRNA. Translation: AAI32658.1 .
BC132659 mRNA. Translation: AAI32660.1 .
CCDSi CCDS21199.1.
PIRi A91005. NGMSG.
RefSeqi NP_032719.1. NM_008693.2.
UniGenei Mm.439740.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1SGF X-ray 3.15 G/Z 25-261 [» ]
ProteinModelPortali P00756.
SMRi P00756. Positions 25-261.
ModBasei Search...

Protein family/group databases

MEROPSi S01.170.

Proteomic databases

MaxQBi P00756.
PaxDbi P00756.
PRIDEi P00756.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000085450 ; ENSMUSP00000082577 ; ENSMUSG00000066515 .
GeneIDi 18050.
KEGGi mmu:18050.
UCSCi uc009gol.2. mouse.

Organism-specific databases

CTDi 18050.
MGIi MGI:97322. Klk1b3.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00750000117405.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi A2RTW1.
KOi K01325.
OMAi NDECDKA.
OrthoDBi EOG75B84T.
PhylomeDBi P00756.
TreeFami TF331065.

Enzyme and pathway databases

BRENDAi 3.4.21.77. 3474.
Reactomei REACT_199000. Activation of Matrix Metalloproteinases.

Miscellaneous databases

EvolutionaryTracei P00756.
NextBioi 293179.
PROi P00756.
SOURCEi Search...

Gene expression databases

ArrayExpressi P00756.
Bgeei P00756.
CleanExi MM_KLK1B3.
Genevestigatori P00756.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a cDNA clone coding for the gamma-subunit of mouse nerve growth factor using a high-stringency selection procedure."
    Ullrich A., Gray A., Wood W.I., Hayflick J., Seeburg P.H.
    DNA 3:387-392(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genes for the alpha and gamma subunits of mouse nerve growth factor are contiguous."
    Evans B.A., Richards R.I.
    EMBO J. 4:133-138(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "The amino acid sequence of the gamma-subunit of mouse submaxillary gland 7 S nerve growth factor."
    Thomas K.A., Baglan N.C., Bradshaw R.A.
    J. Biol. Chem. 256:9156-9166(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-261.
    Tissue: Submandibular gland.
  5. "Structure of mouse 7S NGF: a complex of nerve growth factor with four binding proteins."
    Bax B., Blundell T.L., Murray-Rust J., McDonald N.Q.
    Structure 5:1275-1285(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) OF 7S COMPLEX.
    Strain: Swiss Webster.
    Tissue: Submandibular gland.

Entry informationi

Entry nameiK1KB3_MOUSE
AccessioniPrimary (citable) accession number: P00756
Secondary accession number(s): A2RTW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 3, 2014
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This precursor is cleaved into segments to produce the active form of the gamma chain, which occurs naturally as combinations of either two or three segments held together by disulfide bonds: B1 and A, or B1, C and B2.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi