Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kallikrein 1-related peptidase b3

Gene

Klk1b3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

7S NGF alpha chain stabilizes the 7S complex. The beta dimer promotes neurite growth. The gamma chain is an arginine-specific protease; it may also have plasminogen activator activity, as well as mitogenic activity for chick embryo fibroblasts.

Miscellaneous

This precursor is cleaved into segments to produce the active form of the gamma chain, which occurs naturally as combinations of either two or three segments held together by disulfide bonds: B1 and A, or B1, C and B2.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per 7S complex. The Zn2+ ions are bound at the alpha-gamma interfaces.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay system1
Active sitei120Charge relay system1
Active sitei213Charge relay system1
Metal bindingi231Zinc1
Metal bindingi236Zinc1

GO - Molecular functioni

Keywordsi

Molecular functionGrowth factor, Hydrolase, Protease, Serine protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.21.77 3474

Protein family/group databases

MEROPSiS01.170

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b3 (EC:3.4.21.35)
Alternative name(s):
7S nerve growth factor gamma chain
Gamma-NGF
Glandular kallikrein K3
Short name:
mGK-3
Tissue kallikrein-3
Cleaved into the following 2 chains:
Gene namesi
Name:Klk1b3
Synonyms:Klk-3, Klk3, Ngfg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:97322 Klk1b3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18CuratedAdd BLAST18
PropeptideiPRO_000002796819 – 24Activation peptide1 Publication6
ChainiPRO_000002796925 – 261Kallikrein 1-related peptidase b3Add BLAST237
ChainiPRO_000002797025 – 107Nerve growth factor gamma chain 1Add BLAST83
ChainiPRO_0000027971112 – 261Nerve growth factor gamma chain 2Add BLAST150

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 173
Disulfide bondi50 ↔ 66
Glycosylationi102N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi152 ↔ 219
Disulfide bondi184 ↔ 198
Disulfide bondi209 ↔ 234

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP00756
PaxDbiP00756
PeptideAtlasiP00756
PRIDEiP00756

PTM databases

iPTMnetiP00756

Expressioni

Gene expression databases

BgeeiENSMUSG00000066515
CleanExiMM_KLK1B3
ExpressionAtlasiP00756 baseline and differential
GenevisibleiP00756 MM

Interactioni

Subunit structurei

7S nerve growth factor is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000082577

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi33 – 35Combined sources3
Beta strandi39 – 44Combined sources6
Beta strandi47 – 56Combined sources10
Beta strandi59 – 62Combined sources4
Helixi64 – 66Combined sources3
Beta strandi72 – 76Combined sources5
Beta strandi88 – 97Combined sources10
Helixi103 – 105Combined sources3
Beta strandi122 – 128Combined sources7
Beta strandi133 – 135Combined sources3
Beta strandi151 – 164Combined sources14
Beta strandi172 – 179Combined sources8
Helixi181 – 187Combined sources7
Beta strandi196 – 200Combined sources5
Beta strandi202 – 205Combined sources4
Beta strandi216 – 219Combined sources4
Beta strandi222 – 229Combined sources8
Beta strandi241 – 245Combined sources5
Helixi246 – 249Combined sources4
Helixi250 – 258Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SGFX-ray3.15G/Z25-261[»]
ProteinModelPortaliP00756
SMRiP00756
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00756

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 258Peptidase S1PROSITE-ProRule annotationAdd BLAST234

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 107Segment B1Add BLAST83
Regioni112 – 261Segment AAdd BLAST150
Regioni112 – 164Segment CAdd BLAST53
Regioni165 – 261Segment B2Add BLAST97

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00910000144067
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP00756
KOiK01325
OMAiCMDLKLL
OrthoDBiEOG091G0DF7
PhylomeDBiP00756
TreeFamiTF331065

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00756-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFLILFLAL SLGGIDAAPP VQSRIVGGFK CEKNSQPWHV AVYRYTQYLC
60 70 80 90 100
GGVLLDPNWV LTAAHCYDDN YKVWLGKNNL FKDEPSAQHR FVSKAIPHPG
110 120 130 140 150
FNMSLMRKHI RFLEYDYSND LMLLRLSKPA DITDTVKPIT LPTEEPKLGS
160 170 180 190 200
TCLASGWGSI TPTKFQFTDD LYCVNLKLLP NEDCAKAHIE KVTDAMLCAG
210 220 230 240 250
EMDGGKDTCK GDSGGPLICD GVLQGITSWG HTPCGEPDMP GVYTKLNKFT
260
SWIKDTMAKN P
Length:261
Mass (Da):28,998
Last modified:July 21, 1986 - v1
Checksum:i4870748E174AF7C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti108 – 111Missing (PubMed:3848399).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01389 mRNA Translation: CAA25645.1
X01798 Genomic DNA Translation: CAA25928.1
X01799 Genomic DNA Translation: CAA25930.1
BC132657 mRNA Translation: AAI32658.1
BC132659 mRNA Translation: AAI32660.1
CCDSiCCDS21199.1
PIRiA91005 NGMSG
RefSeqiNP_032719.1, NM_008693.2
UniGeneiMm.439740

Genome annotation databases

EnsembliENSMUST00000085450; ENSMUSP00000082577; ENSMUSG00000066515
GeneIDi18050
KEGGimmu:18050
UCSCiuc009gol.2 mouse

Similar proteinsi

Entry informationi

Entry nameiK1KB3_MOUSE
AccessioniPrimary (citable) accession number: P00756
Secondary accession number(s): A2RTW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 25, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health