P00756 (K1KB3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kallikrein 1-related peptidase b3
EC=3.4.21.35

Alternative name(s):
7S nerve growth factor gamma chain
Gamma-NGF
Glandular kallikrein K3
Short name=mGK-3
Tissue kallikrein-3

Cleaved into the following 2 chains:

1. Nerve growth factor gamma chain 1
2. Nerve growth factor gamma chain 2

Gene names

Name:	Klk1b3
Synonyms:	Klk-3, Klk3, Ngfg

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	7S NGF alpha chain stabilizes the 7S complex. The beta dimer promotes neurite growth. The gamma chain is an arginine-specific protease; it may also have plasminogen activator activity, as well as mitogenic activity for chick embryo fibroblasts.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Cofactor	Binds 2 zinc ions per 7S complex. The zinc ions are bound at the alpha-gamma interfaces.
Subunit structure	7S nerve growth factor is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains.
Miscellaneous	This precursor is cleaved into segments to produce the active form of the gamma chain, which occurs naturally as combinations of either two or three segments held together by disulfide bonds: B1 and A, or B1, C and B2.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Growth factor Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW receptor signaling protein activity Inferred from direct assay. Source: MGI serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

Probable

Propeptide

19 – 24

6

Activation peptide

PRO_0000027968

Chain

25 – 261

237

Kallikrein 1-related peptidase b3

PRO_0000027969

Chain

25 – 107

83

Nerve growth factor gamma chain 1

PRO_0000027970

Chain

112 – 261

150

Nerve growth factor gamma chain 2

PRO_0000027971

Regions

Domain

25 – 258

234

Peptidase S1

Region

25 – 107

83

Segment B1

Region

112 – 261

150

Segment A

Region

112 – 164

53

Segment C

Region

165 – 261

97

Segment B2

Sites

Active site

65

1

Charge relay system

Active site

120

1

Charge relay system

Active site

213

1

Charge relay system

Metal binding

231

1

Zinc

Metal binding

236

1

Zinc

Amino acid modifications

Glycosylation

102

1

N-linked (GlcNAc...) Ref.4

Disulfide bond

31 ↔ 173

Disulfide bond

50 ↔ 66

Disulfide bond

152 ↔ 219

Disulfide bond

184 ↔ 198

Disulfide bond

209 ↔ 234

Experimental info

Sequence conflict

108 – 111

4

Missing Ref.2

Secondary structure

1

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261

Helix Strand Turn

Details...

Beta strand

39 – 44

6

Beta strand

47 – 54

8

Beta strand

56 – 62

7

Helix

64 – 66

3

Beta strand

72 – 76

5

Beta strand

88 – 97

10

Turn

103 – 105

3

Beta strand

122 – 128

7

Beta strand

151 – 158

8

Beta strand

160 – 164

5

Beta strand

172 – 179

8

Helix

181 – 187

7

Beta strand

196 – 200

5

Beta strand

202 – 205

4

Beta strand

216 – 219

4

Beta strand

222 – 229

8

Beta strand

241 – 245

5

Helix

246 – 248

3

Helix

250 – 258

9

Sequences

	Sequence		Length	Mass (Da)
	P00756 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 4870748E174AF7C8	FASTA	261	28,998
	10 20 30 40 50 60 MWFLILFLAL SLGGIDAAPP VQSRIVGGFK CEKNSQPWHV AVYRYTQYLC GGVLLDPNWV 70 80 90 100 110 120 LTAAHCYDDN YKVWLGKNNL FKDEPSAQHR FVSKAIPHPG FNMSLMRKHI RFLEYDYSND 130 140 150 160 170 180 LMLLRLSKPA DITDTVKPIT LPTEEPKLGS TCLASGWGSI TPTKFQFTDD LYCVNLKLLP 190 200 210 220 230 240 NEDCAKAHIE KVTDAMLCAG EMDGGKDTCK GDSGGPLICD GVLQGITSWG HTPCGEPDMP 250 260 GVYTKLNKFT SWIKDTMAKN P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a cDNA clone coding for the gamma-subunit of mouse nerve growth factor using a high-stringency selection procedure." Ullrich A., Gray A., Wood W.I., Hayflick J., Seeburg P.H. DNA 3:387-392(1984) [PubMed: 6548955] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Genes for the alpha and gamma subunits of mouse nerve growth factor are contiguous." Evans B.A., Richards R.I. EMBO J. 4:133-138(1985) [PubMed: 3848399] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"The amino acid sequence of the gamma-subunit of mouse submaxillary gland 7 S nerve growth factor." Thomas K.A., Baglan N.C., Bradshaw R.A. J. Biol. Chem. 256:9156-9166(1981) [PubMed: 7263706] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-261. Tissue: Submandibular gland.
[5]	"Structure of mouse 7S NGF: a complex of nerve growth factor with four binding proteins." Bax B., Blundell T.L., Murray-Rust J., McDonald N.Q. Structure 5:1275-1285(1997) [PubMed: 9351801] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) OF 7S COMPLEX. Strain: Swiss Webster. Tissue: Submandibular gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X01389 mRNA. Translation: CAA25645.1.
X01798 Genomic DNA. Translation: CAA25928.1.
X01799 Genomic DNA. Translation: CAA25930.1.
BC132657 mRNA. Translation: AAI32658.1.
BC132659 mRNA. Translation: AAI32660.1.

IPI

IPI00134582.

PIR

NGMSG. A91005.

RefSeq

NP_032719.1. NM_008693.2.

UniGene

Mm.439740.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SGF	X-ray	3.15	G/Z	25-261	[»]

ProteinModelPortal

P00756.

SMR

P00756. Positions 25-261.

ModBase

Search...

Protein-protein interaction databases

STRING

P00756.

Protein family/group databases

MEROPS

S01.170.

Proteomic databases

PRIDE

P00756.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000085450; ENSMUSP00000082577; ENSMUSG00000066515.

GeneID

18050.

KEGG

mmu:18050.

UCSC

uc009gol.2. mouse.

Organism-specific databases

CTD

18050.

MGI

MGI:97322. Klk1b3.

Phylogenomic databases

HOGENOM

HBG755338.

HOVERGEN

HBG013304.

InParanoid

P00756.

OMA

CINDHYQ.

OrthoDB

EOG4DZ1VW.

PhylomeDB

P00756.

Enzyme and pathway databases

BRENDA

3.4.21.77. 3474.

Gene expression databases

ArrayExpress

P00756.

Bgee

P00756.

CleanEx

MM_KLK1B3.

Genevestigator

P00756.

GermOnline

ENSMUSG00000066515. Mus musculus.

Family and domain databases

InterPro

IPR009003. Pept_cys/ser_Trypsin-like.
IPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
[Graphical view]

KO

K01325.

Pfam

PF00089. Trypsin. 1 hit.
[Graphical view]

PRINTS

PR00722. CHYMOTRYPSIN.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

SUPFAM

SSF50494. Pept_Ser_Cys. 1 hit.

PROSITE

PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

293179.

SOURCE

Search...

Entry information

Entry name

K1KB3_MOUSE

Accession

Primary (citable) accession number: P00756
Secondary accession number(s): A2RTW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 16, 2011
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program