P00755 (K1KB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kallikrein 1-related peptidase b1
EC=3.4.21.35

Alternative name(s):
Glandular kallikrein K1
Short name=mGK-1
Tissue kallikrein-1

Gene names

Name:	Klk1b1
Synonyms:	Klk-1, Klk1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bradykinin biosynthetic process Inferred from mutant phenotype PubMed 11226291. Source: MGI cardiac muscle contraction Inferred from mutant phenotype PubMed 11226291. Source: MGI left ventricular cardiac muscle tissue morphogenesis Inferred from mutant phenotype PubMed 11226291. Source: MGI proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of systemic arterial blood pressure Inferred from mutant phenotype PubMed 11226291. Source: MGI tissue kallikrein-kinin cascade Inferred from mutant phenotype PubMed 11226291PubMed 11282893. Source: MGI vasodilation Inferred from mutant phenotype PubMed 11226291PubMed 11282893. Source: MGI
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 24	6	Activation peptide Probable		PRO_0000027966
	Chain	25 – 261	237	Kallikrein 1-related peptidase b1		PRO_0000027967
Regions
	Domain	25 – 258	234	Peptidase S1
Sites
	Active site	65	1	Charge relay system
	Active site	120	1	Charge relay system
	Active site	213	1	Charge relay system
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Probable
	Disulfide bond	31 ↔ 173		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	152 ↔ 219		By similarity
	Disulfide bond	184 ↔ 198		By similarity
	Disulfide bond	209 ↔ 234		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00755 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 15DC946600AF1604	FASTA	261	29,022
	10 20 30 40 50 60 MWFLILFLAL SLGGIDAAPP VQSRIVGGFK CEKNSQPWHV AVYRYKEYIC GGVLLDANWV 70 80 90 100 110 120 LTAAHCYYEK NNVWLGKNNL YQDEPSAQHR LVSKSFLHPC YNMSLHRNRI QNPQDDYSYD 130 140 150 160 170 180 LMLLRLSKPA DITDVVKPIA LPTEEPKLGS TCLASGWGSI IPVKFQYAKD LQCVNLKLLP 190 200 210 220 230 240 NEDCDKAYVQ KVTDVMLCAG VKGGGKDTCK GDSGGPLICD GVLQGLTSWG YNPCGEPKKP 250 260 GVYTKLIKFT SWIKDTLAQN P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of mouse kallikrein gene family suggests a role in specific processing of biologically active peptides." Mason A.J., Evans B.A., Cox D.R., Shine J., Richards R.I. Nature 303:300-307(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Quakenbush inbred.
[2]	"Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus." Evans B.A., Drinkwater C.C., Richards R.I. J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Salivary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V00829 Genomic DNA. Translation: CAA24213.1. J00390 Genomic DNA. Translation: AAA39349.1. BC026378 mRNA. Translation: AAH26378.1.
IPI	IPI00134580.
PIR	KQMS1. A00941.
RefSeq	NP_034775.1. NM_010645.2.
UniGene	Mm.447819.
3D structure databases
ProteinModelPortal	P00755.
SMR	P00755. Positions 25-261.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000077879.
Protein family/group databases
MEROPS	S01.164.
PTM databases
PhosphoSite	P00755.
Proteomic databases
PaxDb	P00755.
PRIDE	P00755.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000078835; ENSMUSP00000077879; ENSMUSG00000063133.
GeneID	16623.
KEGG	mmu:16623.
UCSC	uc009goc.1. mouse.
Organism-specific databases
CTD	16623.
MGI	MGI:892019. Klk1b1.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00690000101721.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P00755.
KO	K01325.
OrthoDB	EOG415GF4.
Enzyme and pathway databases
BRENDA	3.4.21.35. 3474.
Gene expression databases
Bgee	P00755.
CleanEx	MM_KLK1. MM_KLK1B1.
Genevestigator	P00755.
GermOnline	ENSMUSG00000063133. Mus musculus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	290235.
SOURCE	Search...

Entry information

Entry name

K1KB1_MOUSE

Accession

Primary (citable) accession number: P00755

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program