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P00755 (K1KB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kallikrein 1-related peptidase b1

EC=3.4.21.35
Alternative name(s):
Glandular kallikrein K1
Short name=mGK-1
Tissue kallikrein-1
Gene names
Name:Klk1b1
Synonyms:Klk-1, Klk1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Probable
Propeptide19 – 246Activation peptide Probable
PRO_0000027966
Chain25 – 261237Kallikrein 1-related peptidase b1
PRO_0000027967

Regions

Domain25 – 258234Peptidase S1

Sites

Active site651Charge relay system
Active site1201Charge relay system
Active site2131Charge relay system

Amino acid modifications

Glycosylation1021N-linked (GlcNAc...) Probable
Disulfide bond31 ↔ 173 By similarity
Disulfide bond50 ↔ 66 By similarity
Disulfide bond152 ↔ 219 By similarity
Disulfide bond184 ↔ 198 By similarity
Disulfide bond209 ↔ 234 By similarity

Sequences

Sequence LengthMass (Da)Tools
P00755 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 15DC946600AF1604

FASTA26129,022
        10         20         30         40         50         60 
MWFLILFLAL SLGGIDAAPP VQSRIVGGFK CEKNSQPWHV AVYRYKEYIC GGVLLDANWV 

        70         80         90        100        110        120 
LTAAHCYYEK NNVWLGKNNL YQDEPSAQHR LVSKSFLHPC YNMSLHRNRI QNPQDDYSYD 

       130        140        150        160        170        180 
LMLLRLSKPA DITDVVKPIA LPTEEPKLGS TCLASGWGSI IPVKFQYAKD LQCVNLKLLP 

       190        200        210        220        230        240 
NEDCDKAYVQ KVTDVMLCAG VKGGGKDTCK GDSGGPLICD GVLQGLTSWG YNPCGEPKKP 

       250        260 
GVYTKLIKFT SWIKDTLAQN P 

« Hide

References

« Hide 'large scale' references
[1]"Structure of mouse kallikrein gene family suggests a role in specific processing of biologically active peptides."
Mason A.J., Evans B.A., Cox D.R., Shine J., Richards R.I.
Nature 303:300-307(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Quakenbush inbred.
[2]"Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."
Evans B.A., Drinkwater C.C., Richards R.I.
J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Salivary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V00829 Genomic DNA. Translation: CAA24213.1.
J00390 Genomic DNA. Translation: AAA39349.1.
BC026378 mRNA. Translation: AAH26378.1.
CCDSCCDS21190.1.
PIRKQMS1. A00941.
RefSeqNP_034775.1. NM_010645.2.
UniGeneMm.447819.

3D structure databases

ProteinModelPortalP00755.
SMRP00755. Positions 25-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000077879.

Protein family/group databases

MEROPSS01.164.

PTM databases

PhosphoSiteP00755.

Proteomic databases

MaxQBP00755.
PaxDbP00755.
PRIDEP00755.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000078835; ENSMUSP00000077879; ENSMUSG00000063133.
GeneID16623.
KEGGmmu:16623.
UCSCuc009goc.1. mouse.

Organism-specific databases

CTD16623.
MGIMGI:892019. Klk1b1.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00750000117405.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP00755.
KOK01325.
OrthoDBEOG75B84T.
PhylomeDBP00755.
TreeFamTF331065.

Enzyme and pathway databases

BRENDA3.4.21.35. 3474.

Gene expression databases

BgeeP00755.
CleanExMM_KLK1.
MM_KLK1B1.
GenevestigatorP00755.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio290235.
PROP00755.
SOURCESearch...

Entry information

Entry nameK1KB1_MOUSE
AccessionPrimary (citable) accession number: P00755
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot