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Protein

Glandular kallikrein

Gene
N/A
Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei48Charge relay system1
Active sitei103Charge relay system1
Active sitei198Charge relay system1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Glandular kallikrein (EC:3.4.21.35)
Alternative name(s):
Tissue kallikrein
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3243909.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000279641 – 72 Publications7
ChainiPRO_00000279658 – 246Glandular kallikreinAdd BLAST239

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi14 ↔ 158
Disulfide bondi33 ↔ 49
Glycosylationi85N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi135 ↔ 204
Disulfide bondi169 ↔ 183
Disulfide bondi194 ↔ 219
Glycosylationi239N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP00752.
PRIDEiP00752.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000027121.

Chemistry databases

BindingDBiP00752.

Structurei

Secondary structure

1246
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 27Combined sources6
Beta strandi30 – 39Combined sources10
Beta strandi42 – 45Combined sources4
Helixi47 – 49Combined sources3
Beta strandi55 – 59Combined sources5
Beta strandi61 – 65Combined sources5
Beta strandi71 – 80Combined sources10
Turni82 – 85Combined sources4
Beta strandi105 – 111Combined sources7
Beta strandi134 – 141Combined sources8
Beta strandi157 – 164Combined sources8
Helixi166 – 170Combined sources5
Beta strandi181 – 185Combined sources5
Beta strandi201 – 204Combined sources4
Beta strandi207 – 212Combined sources6
Beta strandi226 – 230Combined sources5
Helixi231 – 234Combined sources4
Helixi235 – 243Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HIAX-ray2.40A/X8-87[»]
B/Y95-246[»]
2KAIX-ray2.50A8-87[»]
B95-246[»]
2PKAX-ray2.05A/X8-87[»]
B/Y95-246[»]
ProteinModelPortaliP00752.
SMRiP00752.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00752.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 243Peptidase S1PROSITE-ProRule annotationAdd BLAST236

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni85 – 104Kallikrein (autolysis) loopAdd BLAST20

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOVERGENiHBG013304.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00752-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
APPIQSRIIG GRECEKNSHP WQVAIYHYSS FQCGGVLVNP KWVLTAAHCK
60 70 80 90 100
NDNYEVWLGR HNLFENENTA QFFGVTADFP HPGFNLSLLK XHTKADGKDY
110 120 130 140 150
SHDLMLLRLQ SPAKITDAVK VLELPTQEPE LGSTCEASGW GSIEPGPDBF
160 170 180 190 200
EFPDEIQCVQ LTLLQNTFCA BAHPBKVTES MLCAGYLPGG KDTCMGDSGG
210 220 230 240
PLICNGMWQG ITSWGHTPCG SANKPSIYTK LIFYLDWIND TITENP
Length:246
Mass (Da):27,172
Last modified:March 27, 2002 - v4
Checksum:i5991CEDE406A19A1
GO

Sequence databases

PIRiA00938. KQPG.

Cross-referencesi

Sequence databases

PIRiA00938. KQPG.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HIAX-ray2.40A/X8-87[»]
B/Y95-246[»]
2KAIX-ray2.50A8-87[»]
B95-246[»]
2PKAX-ray2.05A/X8-87[»]
B/Y95-246[»]
ProteinModelPortaliP00752.
SMRiP00752.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000027121.

Chemistry databases

BindingDBiP00752.
ChEMBLiCHEMBL3243909.

Proteomic databases

PaxDbiP00752.
PRIDEiP00752.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOVERGENiHBG013304.

Miscellaneous databases

EvolutionaryTraceiP00752.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKLK_PIG
AccessioniPrimary (citable) accession number: P00752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 27, 2002
Last modified: November 2, 2016
This is version 122 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Native porcine kallikrein is a monomer. Chains of the pancreatic beta-kallikrein are heterogeneous artifacts of proteolytic degradation during isolation.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.