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Protein

Carboxypeptidase A1

Gene

Cpa1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.

Catalytic activityi

Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi179Zinc; catalyticBy similarity1
Metal bindingi182Zinc; catalyticBy similarity1
Binding sitei237SubstrateBy similarity1
Metal bindingi306Zinc; catalyticBy similarity1
Binding sitei358SubstrateBy similarity1
Active sitei380Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • exopeptidase activity Source: RGD
  • metallocarboxypeptidase activity Source: GO_Central
  • zinc ion binding Source: InterPro

GO - Biological processi

  • proteolysis Source: RGD
  • proteolysis involved in cellular protein catabolic process Source: RGD

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.1. 5301.
SABIO-RKiP00731.

Protein family/group databases

MEROPSiM14.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase A1 (EC:3.4.17.1)
Gene namesi
Name:Cpa1
Synonyms:Cpa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2388. Cpa1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Add BLAST16
PropeptideiPRO_000000435117 – 110Activation peptideAdd BLAST94
ChainiPRO_0000004352111 – 419Carboxypeptidase A1Add BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi248 ↔ 271By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP00731.
PRIDEiP00731.

PTM databases

PhosphoSitePlusiP00731.

Expressioni

Gene expression databases

BgeeiENSRNOG00000010725.
GenevisibleiP00731. RN.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014465.

Structurei

3D structure databases

ProteinModelPortaliP00731.
SMRiP00731.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni179 – 182Substrate bindingBy similarity4
Regioni254 – 255Substrate bindingBy similarity2
Regioni307 – 308Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252967.
HOVERGENiHBG050815.
InParanoidiP00731.
KOiK08779.
OMAiNYAAYHT.
OrthoDBiEOG091G0HUI.
PhylomeDBiP00731.
TreeFamiTF317197.

Family and domain databases

CDDicd03870. M14_CPA. 1 hit.
Gene3Di3.30.70.340. 1 hit.
InterProiView protein in InterPro
IPR034248. CPA_M14_CPD.
IPR003146. M14A_act_pep.
IPR009020. Peptidase/Inhibitor_I9.
IPR000834. Peptidase_M14.
PfamiView protein in Pfam
PF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiView protein in SMART
SM00631. Zn_pept. 1 hit.
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiView protein in PROSITE
PS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00731-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRLLILSLL LEAVCGNENF VGHQVLRISA ADEAQVQKVK ELEDLEHLQL
60 70 80 90 100
DFWRDAARAG IPIDVRVPFP SIQSVKAFLE YHGISYEIMI EDVQLLLDEE
110 120 130 140 150
KQQMSAFQAR ALSTDSFNYA TYHTLDEIYE FMDLLVAEHP QLVSKIQIGN
160 170 180 190 200
TFEGRPIHVL KFSTGGTNRP AIWIDTGIHS REWVTQASGV WFAKKITKDY
210 220 230 240 250
GQDPTFTAVL DNMDIFLEIV TNPDGFAYTH KTNRMWRKTR SHTQGSLCVG
260 270 280 290 300
VDPNRNWDAG FGMAGASSNP CSETYRGKFP NSEVEVKSIV DFVTSHGNIK
310 320 330 340 350
AFISIHSYSQ LLLYPYGYTS EPAPDQAELD QLAKSAVTAL TSLHGTKFKY
360 370 380 390 400
GSIIDTIYQA SGSTIDWTYS QGIKYSFTFE LRDTGLRGFL LPASQIIPTA
410
EETWLALLTI MDHTVKHPY
Length:419
Mass (Da):47,197
Last modified:February 1, 1996 - v2
Checksum:iBB002D1CB99B7491
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti196I → V in CAA24542 (PubMed:6275388).Curated1
Sequence conflicti196I → V in AAA40893 (PubMed:6275388).Curated1
Sequence conflicti261 – 263FGM → LGK in CAA24542 (PubMed:6275388).Curated3
Sequence conflicti261 – 263FGM → LGK in AAA40893 (PubMed:6275388).Curated3
Sequence conflicti347K → E in CAA24542 (PubMed:6275388).Curated1
Sequence conflicti347K → E in AAA40893 (PubMed:6275388).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01232 mRNA. Translation: CAA24542.1.
J00713 mRNA. Translation: AAA40893.1.
M23990
, M23960, M23985, M23986, M23987, M23988, M23989 Genomic DNA. Translation: AAA40955.1.
PIRiA00911. CPRTA.
RefSeqiNP_058694.2. NM_016998.3.
UniGeneiRn.9576.

Genome annotation databases

EnsembliENSRNOT00000014465; ENSRNOP00000014465; ENSRNOG00000010725.
GeneIDi24269.
KEGGirno:24269.
UCSCiRGD:2388. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCBPA1_RAT
AccessioniPrimary (citable) accession number: P00731
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1996
Last modified: May 10, 2017
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families