P00724 (INV2_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 121.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Invertase 2 EC=3.2.1.26 Alternative name(s): Beta-fructofuranosidase 2 Saccharase | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome] | ||||
| Taxonomic identifier | 559292 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › ![]() |
Protein attributes
| Sequence length | 532 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides. |
| Subcellular location | Isoform Intracellular: Cytoplasm Ref.10. |
| Post-translational modification | Isoform Secreted is glycosylated. Isoform Intracellular is not glycosylated. Ref.9 |
| Miscellaneous | Present with 1780 molecules/cell in log phase SD medium. |
| Sequence similarities | Belongs to the glycosyl hydrolase 32 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Secreted |
| Coding sequence diversity | Alternative initiation |
| Domain | Signal |
| Molecular function | Glycosidase Hydrolase |
| PTM | Glycoprotein |
| Technical term | 3D-structure Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | sucrose catabolic process Inferred from direct assay PubMed 363706PubMed 3902817. Source: SGD |
| Cellular_component | extracellular region Inferred from direct assay Ref.5. Source: SGD mitochondrionInferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD |
| Molecular_function | beta-fructofuranosidase activity Inferred from direct assay PubMed 363706PubMed 3902817. Source: SGD sucrose alpha-glucosidase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative initiation. [Align] [Select] | ||||||
| Isoform Secreted (identifier: P00724-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Intracellular (identifier: P00724-2) The sequence of this isoform differs from the canonical sequence as follows: 1-20: Missing. | ||||||
| Note: Produced by alternative initiation at Met-21 of isoform Secreted. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 19 | 19 | Ref.8 | ||||||
| Chain | 20 – 532 | 513 | Invertase 2 | PRO_0000033399 | |||||
Regions | |||||||||
| Region | 39 – 42 | 4 | Substrate binding By similarity | ||||||
| Region | 102 – 103 | 2 | Substrate binding By similarity | ||||||
| Region | 170 – 171 | 2 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 42 | 1 | Ref.8 | ||||||
| Active site | 42 | 1 | By similarity | ||||||
| Binding site | 60 | 1 | Substrate By similarity | ||||||
| Binding site | 223 | 1 | Substrate By similarity | ||||||
| Binding site | 311 | 1 | Substrate By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 23 | 1 | N-linked (GlcNAc...); in isoform Secreted Ref.9 | ||||||
| Glycosylation | 64 | 1 | N-linked (GlcNAc...); in isoform Secreted; partial Ref.9 | ||||||
| Glycosylation | 97 | 1 | N-linked (GlcNAc...); in isoform Secreted Ref.9 | ||||||
| Glycosylation | 111 | 1 | N-linked (GlcNAc...); in isoform Secreted Ref.9 | ||||||
| Glycosylation | 118 | 1 | N-linked (GlcNAc...); in isoform Secreted Ref.9 | ||||||
| Glycosylation | 165 | 1 | N-linked (GlcNAc...); in isoform Secreted; partial Ref.9 | ||||||
| Glycosylation | 266 | 1 | N-linked (GlcNAc...); in isoform Secreted; partial Ref.9 | ||||||
| Glycosylation | 275 | 1 | N-linked (GlcNAc...); in isoform Secreted; partial Ref.9 | ||||||
| Glycosylation | 356 | 1 | N-linked (GlcNAc...); in isoform Secreted Ref.9 | ||||||
| Glycosylation | 369 | 1 | N-linked (GlcNAc...); in isoform Secreted Ref.9 | ||||||
| Glycosylation | 384 | 1 | N-linked (GlcNAc...); in isoform Secreted Ref.9 | ||||||
| Glycosylation | 398 | 1 | N-linked (GlcNAc...); in isoform Secreted Ref.9 | ||||||
| Glycosylation | 512 | 1 | N-linked (GlcNAc...); in isoform Secreted; partial Ref.9 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 20 | 20 | Missing in isoform Intracellular. | VSP_019611 | |||||
Experimental info | |||||||||
| Mutagenesis | 42 | 1 | D → N: Loss of activity. Ref.8 | ||||||
| Sequence conflict | 51 | 1 | K → P in AAA73474. Ref.4 | ||||||
| Sequence conflict | 409 | 1 | A → P AA sequence Ref.9 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequence of the yeast SUC2 gene for invertase." Taussig R., Carlson M. Nucleic Acids Res. 11:1943-1954(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. Barrell B.G.Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [3] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [4] | "[The effects of upstream region of SUC2 gene on its expression]." Xie K.W., Feng B., Li Y.Y. Yi Chuan Xue Bao 18:175-184(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-67. Strain: 33. |
| [5] | "The secreted form of invertase in Saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence." Carlson M., Taussig R., Kustu S., Botstein D. Mol. Cell. Biol. 3:439-447(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-54. |
| [6] | "Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccharomyces cerevisiae." Sarokin L., Carlson M. Mol. Cell. Biol. 4:2750-2757(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30. |
| [7] | "Secretion-defective mutations in the signal sequence for Saccharomyces cerevisiae invertase." Kaiser C.A., Botstein D. Mol. Cell. Biol. 6:2382-2391(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21. |
| [8] | "Identification of an active-site residue in yeast invertase by affinity labeling and site-directed mutagenesis." Reddy V.A., Maley F. J. Biol. Chem. 265:10817-10820(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-27 AND 38-45, ACTIVE SITE ASP-42, MUTAGENESIS OF ASP-42. |
| [9] | "Characterization of the glycosylation sites in yeast external invertase. I. N-linked oligosaccharide content of the individual sequons." Reddy V.A., Johnson R.S., Biemann K., Williams R.S., Ziegler F.D., Trimble R.B., Maley F. J. Biol. Chem. 263:6978-6985(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-532, GLYCOSYLATION AT ASN-23; ASN-64; ASN-97; ASN-111; ASN-118; ASN-165; ASN-266; ASN-275; ASN-356; ASN-369; ASN-384; ASN-398 AND ASN-512. |
| [10] | "Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase." Carlson M., Botstein D. Cell 28:145-154(1982) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF ISOFORMS, SUBCELLULAR LOCATION. |
| [11] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | Z46921 Genomic DNA. Translation: CAA87030.1. U19781 Genomic DNA. Translation: AAA73474.1. V01311 Genomic DNA. Translation: CAA24618.1. K03294 Genomic DNA. Translation: AAA35127.1. M13627 Genomic DNA. Translation: AAA35129.1. BK006942 Genomic DNA. Translation: DAA08390.1. | ||||||||||||
| PIR | IFBY. A00899. | ||||||||||||
| RefSeq | NP_012104.1. NM_001179510.1. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | P00724. | ||||||||||||
| SMR | P00724. Positions 20-531. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| DIP | DIP-5542N. | ||||||||||||
| IntAct | P00724. 2 interactions. | ||||||||||||
| MINT | MINT-477961. | ||||||||||||
Protein family/group databases | |||||||||||||
| CAZy | GH32. Glycoside Hydrolase Family 32. | ||||||||||||
| mycoCLAP | SUC32B_YEAST. | ||||||||||||
PTM databases | |||||||||||||
| GlycoSuiteDB | P00724. | ||||||||||||
2D gel databases | |||||||||||||
| COMPLUYEAST-2DPAGE | P00724. | ||||||||||||
Protocols and materials databases | |||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| EnsemblFungi | YIL162W; YIL162W; YIL162W. | ||||||||||||
| GeneID | 854644. | ||||||||||||
| KEGG | sce:YIL162W. | ||||||||||||
Organism-specific databases | |||||||||||||
| CYGD | YIL162w. | ||||||||||||
| SGD | S000001424. SUC2. | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | HOG000181424. | ||||||||||||
| KO | K01193. | ||||||||||||
| OMA | NPWRSSM. | ||||||||||||
| OrthoDB | EOG4V1B86. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | P00724. | ||||||||||||
| Genevestigator | P00724. | ||||||||||||
| GermOnline | YIL162W. Saccharomyces cerevisiae. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR008985. ConA-like_lec_gl_sf. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_beta-prop. [Graphical view] | ||||||||||||
| Pfam | PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view] | ||||||||||||
| SMART | SM00640. Glyco_32. 1 hit. [Graphical view] | ||||||||||||
| SUPFAM | SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit. | ||||||||||||
| PROSITE | PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other | |||||||||||||
| NextBio | 977181. | ||||||||||||
Entry information
| Entry name | INV2_YEAST | ||||||||
| Accession | Primary (citable) accession number: P00724 Secondary accession number(s): D6VVC4, Q12671 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome IX Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names |
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with
