Reviewed, UniProtKB/Swiss-Prot P00724 (INV2_YEAST)

Names and origin

Protein names

Recommended name:
    Invertase 2
    EC=3.2.1.26
Alternative name(s):
    Beta-fructofuranosidase 2
    Saccharase

Gene names

Name:	SUC2
Ordered Locus Names:	YIL162W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Post-translational modification	The enzyme exists in two states: a glycosylated secreted form, and a non-glycosylated intracellular form. Ref.8
Miscellaneous	Present with 1780 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Coding sequence diversity	Alternative initiation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	sucrose catabolic process Inferred from direct assay. Source: SGD
Cellular component	extracellular region Ref.4 Inferred from direct assay. Source: SGD mitochondrion Inferred from direct assay. Source: SGD
Molecular function	beta-fructofuranosidase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Secreted (identifier: P00724-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Cytoplasmic (identifier: P00724-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.7
	Chain	20 – 532	513	Invertase 2		PRO_0000033399
Regions
	Region	39 – 42	4	Substrate binding By similarity
	Region	102 – 103	2	Substrate binding By similarity
	Region	170 – 171	2	Substrate binding By similarity
Sites
	Active site	42	1	Ref.7
	Active site	42	1	By similarity
	Binding site	60	1	Substrate By similarity
	Binding site	223	1	Substrate By similarity
	Binding site	311	1	Substrate By similarity
Amino acid modifications
	Glycosylation	23	1	N-linked (GlcNAc...); in isoform Secreted Ref.8
	Glycosylation	64	1	N-linked (GlcNAc...); in isoform Secreted; partial Ref.8
	Glycosylation	97	1	N-linked (GlcNAc...); in isoform Secreted Ref.8
	Glycosylation	111	1	N-linked (GlcNAc...); in isoform Secreted Ref.8
	Glycosylation	118	1	N-linked (GlcNAc...); in isoform Secreted Ref.8
	Glycosylation	165	1	N-linked (GlcNAc...); in isoform Secreted; partial Ref.8
	Glycosylation	266	1	N-linked (GlcNAc...); in isoform Secreted; partial Ref.8
	Glycosylation	275	1	N-linked (GlcNAc...); in isoform Secreted; partial Ref.8
	Glycosylation	356	1	N-linked (GlcNAc...); in isoform Secreted Ref.8
	Glycosylation	369	1	N-linked (GlcNAc...); in isoform Secreted Ref.8
	Glycosylation	384	1	N-linked (GlcNAc...); in isoform Secreted Ref.8
	Glycosylation	398	1	N-linked (GlcNAc...); in isoform Secreted Ref.8
	Glycosylation	512	1	N-linked (GlcNAc...); in isoform Secreted; partial Ref.8
Natural variations
	Alternative sequence	1 – 20	20	Missing in isoform Cytoplasmic.		VSP_019611
Experimental info
	Mutagenesis	42	1	D → N: Loss of activity. Ref.7
	Sequence conflict	51	1	K → P in AAA73474. Ref.3
	Sequence conflict	409	1	A → P AA sequence Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Isoform Secreted [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 7D8AB33E6772B775	FASTA	532	60,639
	10 20 30 40 50 60 MLLQAFLFLL AGFAAKISAS MTNETSDRPL VHFTPNKGWM NDPNGLWYDE KDAKWHLYFQ 70 80 90 100 110 120 YNPNDTVWGT PLFWGHATSD DLTNWEDQPI AIAPKRNDSG AFSGSMVVDY NNTSGFFNDT 130 140 150 160 170 180 IDPRQRCVAI WTYNTPESEE QYISYSLDGG YTFTEYQKNP VLAANSTQFR DPKVFWYEPS 190 200 210 220 230 240 QKWIMTAAKS QDYKIEIYSS DDLKSWKLES AFANEGFLGY QYECPGLIEV PTEQDPSKSY 250 260 270 280 290 300 WVMFISINPG APAGGSFNQY FVGSFNGTHF EAFDNQSRVV DFGKDYYALQ TFFNTDPTYG 310 320 330 340 350 360 SALGIAWASN WEYSAFVPTN PWRSSMSLVR KFSLNTEYQA NPETELINLK AEPILNISNA 370 380 390 400 410 420 GPWSRFATNT TLTKANSYNV DLSNSTGTLE FELVYAVNTT QTISKSVFAD LSLWFKGLED 430 440 450 460 470 480 PEEYLRMGFE VSASSFFLDR GNSKVKFVKE NPYFTNRMSV NNQPFKSEND LSYYKVYGLL 490 500 510 520 530 DQNILELYFN DGDVVSTNTY FMTTGNALGS VNMTTGVDNL FYIDKFQVRE VK
	Isoform Cytoplasmic. Checksum: EC78825171A0E8D2	FASTA	512	58,545
	10 20 30 40 50 60 MTNETSDRPL VHFTPNKGWM NDPNGLWYDE KDAKWHLYFQ YNPNDTVWGT PLFWGHATSD 70 80 90 100 110 120 DLTNWEDQPI AIAPKRNDSG AFSGSMVVDY NNTSGFFNDT IDPRQRCVAI WTYNTPESEE 130 140 150 160 170 180 QYISYSLDGG YTFTEYQKNP VLAANSTQFR DPKVFWYEPS QKWIMTAAKS QDYKIEIYSS 190 200 210 220 230 240 DDLKSWKLES AFANEGFLGY QYECPGLIEV PTEQDPSKSY WVMFISINPG APAGGSFNQY 250 260 270 280 290 300 FVGSFNGTHF EAFDNQSRVV DFGKDYYALQ TFFNTDPTYG SALGIAWASN WEYSAFVPTN 310 320 330 340 350 360 PWRSSMSLVR KFSLNTEYQA NPETELINLK AEPILNISNA GPWSRFATNT TLTKANSYNV 370 380 390 400 410 420 DLSNSTGTLE FELVYAVNTT QTISKSVFAD LSLWFKGLED PEEYLRMGFE VSASSFFLDR 430 440 450 460 470 480 GNSKVKFVKE NPYFTNRMSV NNQPFKSEND LSYYKVYGLL DQNILELYFN DGDVVSTNTY 490 500 510 FMTTGNALGS VNMTTGVDNL FYIDKFQVRE VK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the yeast SUC2 gene for invertase." Taussig R., Carlson M. Nucleic Acids Res. 11:1943-1954(1983) [PubMed: 6300785] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"[The effects of upstream region of SUC2 gene on its expression]." Xie K.W., Feng B., Li Y.Y. Yi Chuan Xue Bao 18:175-184(1991) [PubMed: 1888528] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-67. Strain: 33.
[4]	"The secreted form of invertase in Saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence." Carlson M., Taussig R., Kustu S., Botstein D. Mol. Cell. Biol. 3:439-447(1983) [PubMed: 6341817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-54.
[5]	"Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccharomyces cerevisiae." Sarokin L., Carlson M. Mol. Cell. Biol. 4:2750-2757(1984) [PubMed: 6396505] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
[6]	"Secretion-defective mutations in the signal sequence for Saccharomyces cerevisiae invertase." Kaiser C.A., Botstein D. Mol. Cell. Biol. 6:2382-2391(1986) [PubMed: 3537718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
[7]	"Identification of an active-site residue in yeast invertase by affinity labeling and site-directed mutagenesis." Reddy V.A., Maley F. J. Biol. Chem. 265:10817-10820(1990) [PubMed: 2113524] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-27 AND 38-45, ACTIVE SITE ASP-42, MUTAGENESIS OF ASP-42.
[8]	"Characterization of the glycosylation sites in yeast external invertase. I. N-linked oligosaccharide content of the individual sequons." Reddy V.A., Johnson R.S., Biemann K., Williams R.S., Ziegler F.D., Trimble R.B., Maley F. J. Biol. Chem. 263:6978-6985(1988) [PubMed: 3284881] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-532, GLYCOSYLATION AT ASN-23; ASN-64; ASN-97; ASN-111; ASN-118; ASN-165; ASN-266; ASN-275; ASN-356; ASN-369; ASN-384; ASN-398 AND ASN-512.
[9]	"Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase." Carlson M., Botstein D. Cell 28:145-154(1982) [PubMed: 7039847] [Abstract] Cited for: IDENTIFICATION OF ISOFORMS, SUBCELLULAR LOCATION.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46921 Genomic DNA. Translation: CAA87030.1. U19781 Genomic DNA. Translation: AAA73474.1. V01311 Genomic DNA. Translation: CAA24618.1. K03294 Genomic DNA. Translation: AAA35127.1. M13627 Genomic DNA. Translation: AAA35129.1.
PIR	IFBY. A00899.
RefSeq	NP_012104.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5542N.
IntAct	P00724. 6 interactions.
STRING	P00724.
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
PTM databases
GlycoSuiteDB	P00724.
2-D gel databases
COMPLUYEAST-2DPAGE	P00724.
Genome annotation databases
Ensembl	YIL162W; YIL162W; YIL162W; Saccharomyces cerevisiae. [Genome view]
GeneID	854644.
GenomeReviews	Gene locus YIL162W in contig Z47047_GR.
KEGG	sce:YIL162W.
NMPDR	fig|4932.3.peg.1624.
Organism-specific databases
CYGD	YIL162w.
SGD	S000001424. SUC2.
Phylogenomic databases
HOGENOM	P00724.
OMA	QDGLEVT.
Enzyme and pathway databases
BRENDA	3.2.1.26. 250.
Gene expression databases
ArrayExpress	P00724.
Genevestigator	P00724.
GermOnline	YIL162W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977181.

Entry information

Entry name

INV2_YEAST

Accession

Primary (citable) accession number: P00724
Secondary accession number(s): Q12671

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 3, 2009
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)