ID   BGAL_KLULA              Reviewed;        1025 AA.
AC   P00723;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 2.
DT   27-MAR-2024, entry version 153.
DE   RecName: Full=Beta-galactosidase;
DE            Short=Beta-gal;
DE            EC=3.2.1.23;
DE   AltName: Full=Lactase;
GN   Name=LAC4; OrderedLocusNames=KLLA0B14883g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1511885; DOI=10.1016/0378-1119(92)90248-n;
RA   Poch O., L'Hote H., Dallery V., Debeaux F., Fleer R., Sodoyer R.;
RT   "Sequence of the Kluyveromyces lactis beta-galactosidase: comparison with
RT   prokaryotic enzymes and secondary structure analysis.";
RL   Gene 118:55-63(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 /
RC   WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-119.
RX   PubMed=6324114; DOI=10.1093/nar/12.5.2327;
RA   Breunig K.D., Dahlems U., Das S., Hollenberg C.P.;
RT   "Analysis of a eukaryotic beta-galactosidase gene: the N-terminal end of
RT   the yeast Kluyveromyces lactis protein shows homology to the Escherichia
RT   coli lacZ gene product.";
RL   Nucleic Acids Res. 12:2327-2341(1984).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. {ECO:0000305}.
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DR   EMBL; M84410; AAA35265.1; -; Genomic_DNA.
DR   EMBL; CR382122; CAH02587.1; -; Genomic_DNA.
DR   EMBL; X00430; CAA25128.1; -; Genomic_DNA.
DR   PIR; JC1266; JC1266.
DR   RefSeq; XP_452194.1; XM_452194.1.
DR   PDB; 3OB8; X-ray; 2.80 A; A/B/C/D=2-1025.
DR   PDB; 3OBA; X-ray; 2.75 A; A/B/C/D=2-1025.
DR   PDBsum; 3OB8; -.
DR   PDBsum; 3OBA; -.
DR   AlphaFoldDB; P00723; -.
DR   SMR; P00723; -.
DR   STRING; 284590.P00723; -.
DR   ChEMBL; CHEMBL3309040; -.
DR   CAZy; GH2; Glycoside Hydrolase Family 2.
DR   PaxDb; 284590-P00723; -.
DR   GeneID; 2897170; -.
DR   KEGG; kla:KLLA0_B14883g; -.
DR   eggNOG; KOG2024; Eukaryota.
DR   HOGENOM; CLU_002346_0_0_1; -.
DR   InParanoid; P00723; -.
DR   OMA; HYEADIY; -.
DR   BRENDA; 3.2.1.23; 2825.
DR   SABIO-RK; P00723; -.
DR   EvolutionaryTrace; P00723; -.
DR   Proteomes; UP000000598; Chromosome B.
DR   GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR   Gene3D; 2.70.98.10; -; 1.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR   InterPro; IPR004199; B-gal_small/dom_5.
DR   InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR014718; GH-type_carb-bd.
DR   InterPro; IPR006101; Glyco_hydro_2.
DR   InterPro; IPR023232; Glyco_hydro_2_AS.
DR   InterPro; IPR006103; Glyco_hydro_2_cat.
DR   InterPro; IPR023230; Glyco_hydro_2_CS.
DR   InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR   InterPro; IPR006104; Glyco_hydro_2_N.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR032312; LacZ_4.
DR   PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR   PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR   Pfam; PF02929; Bgal_small_N; 1.
DR   Pfam; PF00703; Glyco_hydro_2; 1.
DR   Pfam; PF02836; Glyco_hydro_2_C; 1.
DR   Pfam; PF02837; Glyco_hydro_2_N; 1.
DR   Pfam; PF16353; LacZ_4; 1.
DR   PRINTS; PR00132; GLHYDRLASE2.
DR   SMART; SM01038; Bgal_small_N; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR   SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR   PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
DR   PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Glycosidase; Hydrolase; Reference proteome.
FT   CHAIN           1..1025
FT                   /note="Beta-galactosidase"
FT                   /id="PRO_0000057663"
FT   ACT_SITE        482
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        551
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   HELIX           7..9
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          16..18
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   TURN            29..31
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          32..34
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          37..46
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           47..49
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           53..55
FT                   /evidence="ECO:0007829|PDB:3OB8"
FT   HELIX           58..61
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          66..70
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           77..79
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          85..90
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          106..115
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           117..122
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          123..130
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          132..140
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          143..149
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          155..158
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   TURN            160..162
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          165..176
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           179..183
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          187..190
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          198..220
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          225..237
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          241..247
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           258..262
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          275..280
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          287..295
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          301..304
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          306..314
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          320..328
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          333..336
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          339..342
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          349..353
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   TURN            359..361
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           367..379
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          384..386
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           396..403
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          406..410
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           416..419
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           420..425
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   TURN            433..436
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           437..440
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   TURN            441..443
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           444..446
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   TURN            447..449
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           451..453
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           454..468
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          474..478
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           487..499
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          503..506
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          517..523
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           526..536
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          543..545
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          547..553
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           563..572
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          576..582
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          589..594
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   TURN            596..598
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          599..606
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   TURN            608..611
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           618..621
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           634..642
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          645..651
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          654..659
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          669..673
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   TURN            674..676
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          690..694
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          698..706
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          716..721
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          741..744
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          746..753
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          756..761
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   TURN            762..765
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          766..772
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          775..779
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          785..787
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   TURN            794..799
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           800..806
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           809..811
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          812..823
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          829..840
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          846..855
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          857..872
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          878..886
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          890..898
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          913..917
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           918..920
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          935..945
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   TURN            946..948
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          949..958
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          960..968
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   TURN            970..972
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           976..978
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          980..993
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           999..1001
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   HELIX           1007..1009
FT                   /evidence="ECO:0007829|PDB:3OBA"
FT   STRAND          1016..1024
FT                   /evidence="ECO:0007829|PDB:3OBA"
SQ   SEQUENCE   1025 AA;  117619 MW;  AF3136235DB5A464 CRC64;
     MSCLIPENLR NPKKVHENRL PTRAYYYDQD IFESLNGPWA FALFDAPLDA PDAKNLDWET
     AKKWSTISVP SHWELQEDWK YGKPIYTNVQ YPIPIDIPNP PTVNPTGVYA RTFELDSKSI
     ESFEHRLRFE GVDNCYELYV NGQYVGFNKG SRNGAEFDIQ KYVSEGENLV VVKVFKWSDS
     TYIEDQDQWW LSGIYRDVSL LKLPKKAHIE DVRVTTTFVD SQYQDAELSV KVDVQGSSYD
     HINFTLYEPE DGSKVYDASS LLNEENGNTT FSTKEFISFS TKKNEETAFK INVKAPEHWT
     AENPTLYKYQ LDLIGSDGSV IQSIKHHVGF RQVELKDGNI TVNGKDILFR GVNRHDHHPR
     FGRAVPLDFV VRDLILMKKF NINAVRNSHY PNHPKVYDLF DKLGFWVIDE ADLETHGVQE
     PFNRHTNLEA EYPDTKNKLY DVNAHYLSDN PEYEVAYLDR ASQLVLRDVN HPSIIIWSLG
     NEACYGRNHK AMYKLIKQLD PTRLVHYEGD LNALSADIFS FMYPTFEIME RWRKNHTDEN
     GKFEKPLILC EYGHAMGNGP GSLKEYQELF YKEKFYQGGF IWEWANHGIE FEDVSTADGK
     LHKAYAYGGD FKEEVHDGVF IMDGLCNSEH NPTPGLVEYK KVIEPVHIKI AHGSVTITNK
     HDFITTDHLL FIDKDTGKTI DVPSLKPEES VTIPSDTTYV VAVLKDDAGV LKAGHEIAWG
     QAELPLKVPD FVTETAEKAA KINDGKRYVS VESSGLHFIL DKLLGKIESL KVKGKEISSK
     FEGSSITFWR PPTNNDEPRD FKNWKKYNID LMKQNIHGVS VEKGSNGSLA VVTVNSRISP
     VVFYYGFETV QKYTIFANKI NLNTSMKLTG EYQPPDFPRV GYEFWLGDSY ESFEWLGRGP
     GESYPDKKES QRFGLYDSKD VEEFVYDYPQ ENGNHTDTHF LNIKFEGAGK LSIFQKEKPF
     NFKISDEYGV DEAAHACDVK RYGRHYLRLD HAIHGVGSEA CGPAVLDQYR LKAQDFNFEF
     DLAFE
//